Restriction map for
pFN21AA0094
(Circular) (Six-base) MAPSORT of: pFN21AA0094.seq Check: 5682 from: 1 to: 5863
pFN21AA0094 5863 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2424 2514 3926 5310 5578 1632
Size: 792 90 1412 1384 268 1917
Fragments arranged by size:
1917 1412 1384 792 268 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2554 1184
Size: 1370 4493
AlwNI CAG_nnn'CTG
Cuts at: 3101 3606 5178 3101
Size: 505 1572 3786
Fragments arranged by size:
3786 1572 505
ApaLI G'TGCA_C
Cuts at: 3135 3920 5076 3135
Size: 785 1156 3922
Fragments arranged by size:
3922 1156 785
ApoI r'AATT_y
Cuts at: 1493 3172 3398 3550 5659 1493
Size: 1679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2223 4417 160
Size: 872 1191 2194 1606
Fragments arranged by size:
2194 1606 1191 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 3188 1870
Size: 73 1245 4545
Fragments arranged by size:
4545 1245 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 3182 1391
Size: 711 1080 4072
Fragments arranged by size:
4072 1080 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2190 928
Size: 971 291 4601
Fragments arranged by size:
4601 971 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 2332 1548
Size: 784 5079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2293 4474
Size: 107 122 71 631 605 337 284 2181
Cuts at: 4474 136
Size: 1525
Fragments arranged by size:
2181 1525 631 605 337 284 122 107
71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 3139 3924 5080 1790
Size: 1349 785 1156 2573
Fragments arranged by size:
2573 1349 1156 785
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3594 4552 442
Size: 416 53 83 399 24 2177 958 1753
Fragments arranged by size:
2177 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 4523 1239
Size: 809 2475 2579
Fragments arranged by size:
2579 2475 809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3988 4853 5151 3988
Size: 865 298 4700
Fragments arranged by size:
4700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5863
BseYI C'CCAG_C
Cuts at: 2086 5066 5562 2086
Size: 2980 496 2387
Fragments arranged by size:
2980 2387 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3313 4074 5102 1540
Size: 275 186 12 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2740 3081 3139 3924 4009 5080 2740
Size: 341 58 785 85 1071 3523
Fragments arranged by size:
3523 1071 785 341 85 58
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 3045 2012
Size: 1033 4830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2740 3081 3139 3182 3924 4009
Size: 399 312 638 341 58 43 742 85
Cuts at: 4009 5080 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 638 399 341 312 85
58 43
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 3218 844
Size: 1387 987 3489
Fragments arranged by size:
3489 1387 987
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2686 4358 4532 65
Size: 1183 1438 1672 174 1396
Fragments arranged by size:
1672 1438 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2465 4507 4694 1700
Size: 110 655 2042 187 2869
Fragments arranged by size:
2869 2042 655 187 110
BsrGI T'GTAC_A
Cuts at: 96 2512 96
Size: 2416 3447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2528 794
Size: 1734 4129
BstBI TT'CG_AA
Cuts at: 2869 4667 2869
Size: 1798 4065
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2852 3033 2852
Size: 181 5682
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2341 2341
Size: 5863
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2234 2611 513
Size: 552 939 9 221 377 3765
Fragments arranged by size:
3765 939 552 377 221 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2796 3379 4173 4193 2056
Size: 740 583 794 20 3726
Fragments arranged by size:
3726 794 740 583 20
ClaI AT'CG_AT
Cuts at: 1554 2248 1554
Size: 694 5169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 2128 3167 2128
Size: 1039 4824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3023 3310
Size: 54 1425 50 275 18 180 1013 287
Cuts at: 3310 4199 5828 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1013 889 287 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3310 1537
Size: 275 198 1300 4090
Fragments arranged by size:
4090 1300 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 1353
Size: 2440 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3018 4450 4824 4970 387
Size: 1064 138 1429 1432 374 146 1280
Fragments arranged by size:
1432 1429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2424 2514 3926 4523 5310 5578
Size: 393 416 376 90 1412 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 1412 787 597 416 393 376 268
90
EcoO109I rG'GnC_Cy
Cuts at: 2631 2695 2631
Size: 64 5799
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
EcoRV GAT'ATC
Cuts at: 2665 2665
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373
Size: 32 135 32 2932 32 42 32 2626
Fragments arranged by size:
2932 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2897 748
Size: 2149 3714
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 2979 3462 2979
Size: 483 5380
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2698 2929 3014 4977 5161 1305
Size: 1393 231 85 1963 184 2007
Fragments arranged by size:
2007 1963 1393 231 184 85
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3025 10
Size: 54 1425 343 1193 2848
Fragments arranged by size:
2848 1425 1193 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2764 3821 4180 4339 518
Size: 648 1598 1057 359 159 2042
Fragments arranged by size:
2042 1598 1057 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3279 3956 5104 5349 1606
Size: 1673 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 677 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 5863
NcoI C'CATG_G
Cuts at: 513 1065 2004 2234 2611 513
Size: 552 939 230 377 3765
Fragments arranged by size:
3765 939 552 377 230
NdeI CA'TA_TG
Cuts at: 387 2761 387
Size: 2374 3489
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2526 2843 3221 4766 1132
Size: 97 1297 317 378 1545 2229
Fragments arranged by size:
2229 1545 1297 378 317 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PfoI T'CCnGG_A
Cuts at: 2201 2201
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5863
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 2565 3433 2565
Size: 868 4995
PstI C_TGCA'G
Cuts at: 830 2048 3215 830
Size: 1218 1167 3478
Fragments arranged by size:
3478 1218 1167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SexAI A'CCwGG_T
Cuts at: 2479 2479
Size: 5863
SfcI C'TryA_G
Cuts at: 826 1045 2044 3211 4346 5027 5218 826
Size: 219 999 1167 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 999 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3967 4868 5130 820
Size: 197 926 2024 901 262 1553
Fragments arranged by size:
2024 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 2843 3221 1132
Size: 1711 378 3774
Fragments arranged by size:
3774 1711 378
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2004 2234 2611 3302 513
Size: 552 939 230 377 691 3074
Fragments arranged by size:
3074 939 691 552 377 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3890 3907 4060 4245 4690 325
Size: 1255 2310 17 153 185 445 1498
Fragments arranged by size:
2310 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2512 4109 5821
Size: 274 80 33 51 1443 535 1597 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
1712 1597 1443 535 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI