Restriction map for
pFN21AA0092
(Circular) (Six-base) MAPSORT of: pFN21AA0092.seq Check: 9415 from: 1 to: 6127
pFN21AA0092 6127 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3344 4190 5574 5842 1632
Size: 1712 846 1384 268 1917
Fragments arranged by size:
1917 1712 1384 846 268
AflII C'TTAA_G
Cuts at: 820 1017 2222 820
Size: 197 1205 4725
Fragments arranged by size:
4725 1205 197
AflIII A'CryG_T
Cuts at: 1203 2545 5026 6003 1203
Size: 1342 2481 977 1327
Fragments arranged by size:
2481 1342 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2634 1184
Size: 1450 4677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2105 2073
Size: 32 6095
AlwNI CAG_nnn'CTG
Cuts at: 3870 5442 3870
Size: 1572 4555
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2353 3035 3391 3436 3662 3814 5923 1493
Size: 860 682 356 45 226 152 2109 1697
Fragments arranged by size:
2109 1697 860 682 356 226 152 45
AseI AT'TA_AT
Cuts at: 160 1032 2994 4681 160
Size: 872 1962 1687 1606
Fragments arranged by size:
1962 1687 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3446 1391
Size: 2055 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 2536 3055 1548
Size: 988 519 4620
Fragments arranged by size:
4620 988 519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4738 136
Size: 107 122 71 631 605 337 2729 1525
Fragments arranged by size:
2729 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2061 3544 2061
Size: 1483 4644
Bme1580I G_kGCm'C
Cuts at: 1790 4188 5344 1790
Size: 2398 1156 2573
Fragments arranged by size:
2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3221 3357 4787 1239
Size: 1982 136 1430 2579
Fragments arranged by size:
2579 1982 1430 136
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 4252 5117 5415 2279
Size: 1973 865 298 2991
Fragments arranged by size:
2991 1973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3325 4790 882
Size: 617 1826 1465 2219
Fragments arranged by size:
2219 1826 1465 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3934 4316 4951
Size: 53 83 186 936 482 1919 382 635
Cuts at: 4951 275
Size: 1451
Fragments arranged by size:
1919 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2971 3014 1186
Size: 1785 43 4299
Fragments arranged by size:
4299 1785 43
BseYI C'CCAG_C
Cuts at: 3195 5330 5826 3195
Size: 2135 496 3496
Fragments arranged by size:
3496 2135 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4188 4273 5344 4188
Size: 85 1071 4971
Fragments arranged by size:
4971 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3638 3731 2095
Size: 1543 93 4491
Fragments arranged by size:
4491 1543 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3446 4188 4273 5344 1391
Size: 399 1656 742 85 1071 2174
Fragments arranged by size:
2174 1656 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2899 4622 4796 65
Size: 1183 1651 1723 174 1396
Fragments arranged by size:
1723 1651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 2940 4187 5199 2940
Size: 1247 1012 3868
Fragments arranged by size:
3868 1247 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 3181 4931 3181
Size: 1750 4377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2572 3457 3824 4209 4226 5678 5764 5776
Size: 600 885 367 385 17 1452 86 12
Cuts at: 5776 6122 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 885 600 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2832 513
Size: 552 939 828 3808
Fragments arranged by size:
3808 939 828 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DraIII CAC_nnn'GTG
Cuts at: 2945 2945
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092
Size: 54 1425 50 275 18 1744 889 1629
Cuts at: 6092 8
Size: 43
Fragments arranged by size:
1744 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3221 3344 3357 4190 4787 5574 5842
Size: 393 1589 123 13 833 597 787 268
Cuts at: 5842 1239
Size: 1524
Fragments arranged by size:
1589 1524 833 787 597 393 268 123
13
EcoO109I rG'GnC_Cy
Cuts at: 2614 2614
Size: 6127
EcoRI G'AATT_C
Cuts at: 2353 3391 3436 3814 2353
Size: 1038 45 378 4666
Fragments arranged by size:
4666 1038 378 45
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2990 3022 3040
Size: 32 135 32 902 32 484 32 18
Cuts at: 3040 3072 3340 3372 4768 4800 4842 4874 1373
Size: 32 268 32 1396 32 42 32 2626
Fragments arranged by size:
2626 1396 902 484 268 135 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2883 748
Size: 2135 3992
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 2307 2441 3726 2307
Size: 134 1285 4708
Fragments arranged by size:
4708 1285 134
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 3009 3096 5241 5425 1305
Size: 1317 387 87 2145 184 2007
Fragments arranged by size:
2145 2007 1317 387 184 87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4085 4444 4603 518
Size: 648 2919 359 159 2042
Fragments arranged by size:
2919 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2699 3199 3543 4220 5368 5613 1606
Size: 1093 500 344 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 677 500 344 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4636
Fragments arranged by size:
4636 939 552
NdeI CA'TA_TG
Cuts at: 387 2110 387
Size: 1723 4404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2455 2474 2549 2686 3379 3485 5030
Size: 97 1226 19 75 137 693 106 1545
Cuts at: 5030 1132
Size: 2229
Fragments arranged by size:
2229 1545 1226 693 137 106 97 75
19
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 2545 5026 2545
Size: 2481 3646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 1108
Size: 1725 4402
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2492 1613
Size: 879 5248
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2829 2861 2829
Size: 32 6095
PstI C_TGCA'G
Cuts at: 830 2413 3479 830
Size: 1583 1066 3478
Fragments arranged by size:
3478 1583 1066
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2699 3199 2699
Size: 500 5627
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 2325 4375 6087 2325
Size: 2050 1712 2365
Fragments arranged by size:
2365 2050 1712
SfcI C'TryA_G
Cuts at: 826 1045 2409 3475 4610 5291 5482 826
Size: 219 1364 1066 1135 681 191 1471
Fragments arranged by size:
1471 1364 1135 1066 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2258 4231 5132 5394 820
Size: 197 926 279 36 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 279 262 197 36
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 2474 2686 3485 1132
Size: 1342 212 799 3774
Fragments arranged by size:
3774 1342 799 212
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 3566 513
Size: 552 939 1562 3074
Fragments arranged by size:
3074 1562 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2323 4373 6085
Size: 274 80 33 51 1443 346 2050 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
2050 1712 1443 346 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 2297 2716 3463 2297
Size: 419 747 4961
Fragments arranged by size:
4961 747 419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI