Restriction map for
pFN21AA0089
(Circular) (Six-base) MAPSORT of: pFN21AA0089.seq Check: 7755 from: 1 to: 5758
pFN21AA0089 5758 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 3101 1937
Size: 816 348 4594
Fragments arranged by size:
4594 816 348
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2943 3020 3821 5205 5473 1632
Size: 449 862 77 801 1384 268 1917
Fragments arranged by size:
1917 1384 862 801 449 268 77
AfeI AGC'GCT
Cuts at: 2349 2349
Size: 5758
AflII C'TTAA_G
Cuts at: 820 1017 2618 820
Size: 197 1601 3960
Fragments arranged by size:
3960 1601 197
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2934 1184
Size: 1750 4008
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 2038
Size: 32 5726
AlwNI CAG_nnn'CTG
Cuts at: 2313 2597 3501 5073 2313
Size: 284 904 1572 2998
Fragments arranged by size:
2998 1572 904 284
ApaI G_GGCC'C
Cuts at: 2387 2387
Size: 5758
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2102 2563 3067 3293 3445 5554 1493
Size: 609 461 504 226 152 2109 1697
Fragments arranged by size:
2109 1697 609 504 461 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2387 3083 1870
Size: 73 98 346 696 4545
Fragments arranged by size:
4545 696 346 98 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 3079 4534 618
Size: 325 217 627 227 1065 1455 1842
Fragments arranged by size:
1842 1455 1065 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2042 2387 3077 1391
Size: 651 345 690 4072
Fragments arranged by size:
4072 690 651 345
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 5758
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 3026 928
Size: 971 962 165 3660
Fragments arranged by size:
3660 971 962 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 2793 1548
Size: 1245 4513
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2991 5753 2991
Size: 2762 2996
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2387 3819 4975 1790
Size: 550 47 1432 1156 2573
Fragments arranged by size:
2573 1432 1156 550 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 2337 2859
Size: 416 53 83 399 24 642 278 522
Cuts at: 2859 3489 4447 442
Size: 630 958 1753
Fragments arranged by size:
1753 958 642 630 522 416 399 278
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4418 1239
Size: 3179 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4421 882
Size: 617 993 1929 2219
Fragments arranged by size:
2219 1929 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3565 3947 4582
Size: 53 83 186 936 482 1550 382 635
Cuts at: 4582 275
Size: 1451
Fragments arranged by size:
1550 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 2350 2642 4961 5457 2350
Size: 292 2319 496 2651
Fragments arranged by size:
2651 2319 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 4642
Fragments arranged by size:
4642 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2688 3208 3969 4997 1540
Size: 275 186 687 520 761 1028 2301
Fragments arranged by size:
2301 1028 761 687 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2340 2387 3077 3819 3904 4975
Size: 399 252 298 47 690 742 85 1071
Cuts at: 4975 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 690 399 298 252 85
47
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 3113 844
Size: 1436 833 3489
Fragments arranged by size:
3489 1436 833
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2463 4253 4427 65
Size: 1183 1215 1790 174 1396
Fragments arranged by size:
1790 1396 1215 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 2569 4562 2569
Size: 1993 3765
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2730 2991 3088 3455 3840 3857 5309 5395
Size: 758 261 97 367 385 17 1452 86
Cuts at: 5395 5407 5753 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 261 97
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2988 3274 4068 4088 2988
Size: 286 794 20 4658
Fragments arranged by size:
4658 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 2784 2829
Size: 54 1425 50 275 18 855 99 45
Cuts at: 2829 3205 4094 5723 8
Size: 376 889 1629 43
Fragments arranged by size:
1629 1425 889 855 376 275 99 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2685 3205 1537
Size: 275 873 520 4090
Fragments arranged by size:
4090 873 520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2859 3688 1353
Size: 1190 316 829 3423
Fragments arranged by size:
3423 1190 829 316
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2684 2830 4345 4719 4865 387
Size: 1064 138 1095 146 1515 374 146 1280
Fragments arranged by size:
1515 1280 1095 1064 374 146 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2943 3020 3821 4418 5205 5473
Size: 393 449 862 77 801 597 787 268
Cuts at: 5473 1239
Size: 1524
Fragments arranged by size:
1524 862 801 787 597 449 393 268
77
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2383 2384 2383
Size: 1 5757
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2018 2351 4905 1678
Size: 340 333 2554 2531
Fragments arranged by size:
2554 2531 340 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3011 3043 4399 4431 4473
Size: 32 135 32 1439 32 1356 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 1439 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2850 4872 5056 1305
Size: 1545 2022 184 2007
Fragments arranged by size:
2022 2007 1545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2444 2711 3716 4075 4234 518
Size: 648 1021 257 267 1005 359 159 2042
Fragments arranged by size:
2042 1021 1005 648 359 267 257 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2060 2664 3174 3851 4999 5244 1606
Size: 406 48 604 510 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 510 406 245 48
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 5758
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PfoI T'CCnGG_A
Cuts at: 2203 2203
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2383 2383
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2485 2556 2983 3110 830
Size: 1655 71 427 127 3478
Fragments arranged by size:
3478 1655 427 127 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2060 2060
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2481 2552 2754 2979 3106 4241 4922
Size: 219 1436 71 202 225 127 1135 681
Cuts at: 4922 5113 826
Size: 191 1471
Fragments arranged by size:
1471 1436 1135 681 225 219 202 191
127 71
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3862 4763 5025 820
Size: 197 926 675 1244 901 262 1553
Fragments arranged by size:
1553 1244 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 1517
Size: 1320 4438
StyI C'CwwG_G
Cuts at: 513 1065 2004 2902 3197 513
Size: 552 939 898 295 3074
Fragments arranged by size:
3074 939 898 552 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2866 2347
Size: 519 5239
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI