Restriction map for
pFN21AA0078
(Circular) (Six-base) MAPSORT of: pFN21AA0078.seq Check: 2229 from: 1 to: 6598
pFN21AA0078 6598 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2358 3182 3689 4661 6045 6313 1632
Size: 726 824 507 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 824 726 507 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 2547 5497 6474 1203
Size: 1344 2950 977 1327
Fragments arranged by size:
2950 1344 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AlwNI CAG_nnn'CTG
Cuts at: 2365 3464 4341 5913 2365
Size: 1099 877 1572 3050
Fragments arranged by size:
3050 1572 1099 877
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 2052 2342 3248 3260 3907 4133 4285 6394
Size: 559 290 906 12 647 226 152 2109
Cuts at: 6394 1493
Size: 1697
Fragments arranged by size:
2109 1697 906 647 559 290 226 152
12
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3923 1870
Size: 73 1980 4545
Fragments arranged by size:
4545 1980 73
BamHI G'GATC_C
Cuts at: 2848 3928 4295 2848
Size: 1080 367 5151
Fragments arranged by size:
5151 1080 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 3919 5374 618
Size: 325 217 627 1277 855 1455 1842
Fragments arranged by size:
1842 1455 1277 855 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3022 3333 3917 1391
Size: 1631 311 584 4072
Fragments arranged by size:
4072 1631 584 311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 3695 928
Size: 971 1308 488 3831
Fragments arranged by size:
3831 1308 971 488
BbvCI CC'TCA_GC
Cuts at: 3477 3477
Size: 6598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4490 5700 4490
Size: 1210 5388
BclI T'GATC_A
Cuts at: 1548 2356 2878 1548
Size: 808 522 5268
Fragments arranged by size:
5268 808 522
BfrBI ATG'CAT
Cuts at: 3635 3635
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5209 136
Size: 107 122 71 631 605 3537 1525
Fragments arranged by size:
3537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 3135 4015 3135
Size: 880 5718
Bme1580I G_kGCm'C
Cuts at: 1790 4659 5815 1790
Size: 2869 1156 2573
Fragments arranged by size:
2869 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 4329 5287
Size: 416 53 83 399 24 778 2134 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
2134 1753 958 778 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2063 1056
Size: 1007 5591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3341 3747 5258 1239
Size: 2102 406 1511 2579
Fragments arranged by size:
2579 2102 1511 406
Bpu10I CC'TnA_GC
Cuts at: 3477 3477
Size: 6598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3770 4723 5588 5886 3770
Size: 953 865 298 4482
Fragments arranged by size:
4482 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5261 882
Size: 617 3762 2219
Fragments arranged by size:
3762 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4389 493
Size: 711 3185 2702
Fragments arranged by size:
3185 2702 711
BsaBI GATnn'nnATC
Cuts at: 3651 6592 3651
Size: 2941 3657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5031 5429 5703 5850 6261 5031
Size: 398 274 147 411 5368
Fragments arranged by size:
5368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6373
Fragments arranged by size:
6373 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3120 3240 3465 1186
Size: 1934 120 225 4319
Fragments arranged by size:
4319 1934 225 120
BseYI C'CCAG_C
Cuts at: 2394 5801 6297 2394
Size: 3407 496 2695
Fragments arranged by size:
3407 2695 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2766 1163
Size: 573 1030 4995
Fragments arranged by size:
4995 1030 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2600 3715 4659 4744 5815 2600
Size: 1115 944 85 1071 3383
Fragments arranged by size:
3383 1115 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2246 4109 4202 2246
Size: 1863 93 4642
Fragments arranged by size:
4642 1863 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2600 3022 3333 3715 3917 4659 4744
Size: 399 810 422 311 382 202 742 85
Cuts at: 4744 5815 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 810 742 422 399 382 311
202 85
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 2347 4487 6217 2347
Size: 2140 1730 2728
Fragments arranged by size:
2728 2140 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 3139 3472 3953 844
Size: 1454 841 333 481 3489
Fragments arranged by size:
3489 1454 841 481 333
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5242 5429 1700
Size: 110 3432 187 2869
Fragments arranged by size:
3432 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6598
BstBI TT'CG_AA
Cuts at: 2197 5402 2197
Size: 3205 3393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstYI r'GATC_y
Cuts at: 1972 2848 3058 3928 4295 4680 4697 6149 6235
Size: 876 210 870 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 876 870 385 367 346 210
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2295 3423 2295
Size: 1128 5470
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3850 487
Size: 683 56 2624 3235
Fragments arranged by size:
3235 2624 683 56
BtsI GCAGTG_nn'
Cuts at: 2363 2533 4114 4908 4928 2363
Size: 170 1581 794 20 4033
Fragments arranged by size:
4033 1581 794 170 20
ClaI AT'CG_AT
Cuts at: 1554 2385 3324 1554
Size: 831 939 4828
Fragments arranged by size:
4828 939 831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2273 4045
Size: 54 1425 50 275 18 216 227 1772
Cuts at: 4045 4934 6563 8
Size: 889 1629 43
Fragments arranged by size:
1772 1629 1425 889 275 227 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 2960 3336 3609 3615 4528 1353
Size: 1068 539 376 273 6 913 3423
Fragments arranged by size:
3423 1068 913 539 376 273 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5185 5559 5705 387
Size: 1064 138 3596 374 146 1280
Fragments arranged by size:
3596 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 3182 3341 3689 3747 4661 5258
Size: 393 726 824 159 348 58 914 597
Cuts at: 5258 6045 6313 1239
Size: 787 268 1524
Fragments arranged by size:
1524 914 824 787 726 597 393 348
268 159 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2750 2294
Size: 456 6142
EcoO109I rG'GnC_Cy
Cuts at: 2039 2039
Size: 6598
EcoRI G'AATT_C
Cuts at: 3248 3907 4285 3248
Size: 659 378 5561
Fragments arranged by size:
5561 659 378
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 5745 1678
Size: 4067 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2798 2830 3460 3492 5239
Size: 32 135 32 1226 32 630 32 1747
Cuts at: 5239 5271 5313 5345 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1747 1226 630 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 2983 3942 4188 669
Size: 988 281 349 696 959 246 3079
Fragments arranged by size:
3079 988 959 696 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 5712 5896 1305
Size: 1674 2733 184 2007
Fragments arranged by size:
2733 2007 1674 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 4560
Fragments arranged by size:
4560 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 4556 4915 5074 518
Size: 648 1221 2169 359 159 2042
Fragments arranged by size:
2169 2042 1221 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4014 4691 5839 6084 1606
Size: 2408 677 1148 245 2120
Fragments arranged by size:
2408 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6046
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 2059 1052
Size: 1007 5591
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NsiI A_TGCA'T
Cuts at: 3637 3637
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2551 3956 5501 1132
Size: 97 1322 1405 1545 2229
Fragments arranged by size:
2229 1545 1405 1322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 2547 5497 2547
Size: 2950 3648
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2055 1108
Size: 947 5651
PfoI T'CCnGG_A
Cuts at: 3357 3357
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 2319 4168 2319
Size: 1849 4749
PstI C_TGCA'G
Cuts at: 830 3950 830
Size: 3120 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 3336 3336
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 3507 3796 3946 5081 5762 5953 826
Size: 219 2462 289 150 1135 681 191 1471
Fragments arranged by size:
2462 1471 1135 681 289 219 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3749 4702 5603 5865 820
Size: 197 926 1806 953 901 262 1553
Fragments arranged by size:
1806 1553 953 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 2688 4522 5
Size: 47 2636 1834 2081
Fragments arranged by size:
2636 2081 1834 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 3881 4037 513
Size: 552 2816 156 3074
Fragments arranged by size:
3074 2816 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 4625 4642 4795 4980 5425 325
Size: 1255 1230 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 1230 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4844 6556 96
Size: 274 80 33 51 1443 2867 1712 138
Fragments arranged by size:
2867 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6558 6590 6558
Size: 32 6566
Tth111I GACn'n_nGTC
Cuts at: 1316 2987 1316
Size: 1671 4927
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 2343 4727 2343
Size: 2384 4214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI