Restriction map for
pFN21AA0055
(Circular) (Six-base) MAPSORT of: pFN21AA0055.seq Check: 2736 from: 1 to: 8059
pFN21AA0055 8059 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2727 3070 5049 2727
Size: 343 1979 5737
Fragments arranged by size:
5737 1979 343
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5869 278
Size: 53 83 186 936 4333 2468
Fragments arranged by size:
4333 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 5402 1937
Size: 999 2466 4594
Fragments arranged by size:
4594 2466 999
Acc65I G'GTAC_C
Cuts at: 5380 5380
Size: 8059
AclI AA'CG_TT
Cuts at: 1563 5041 6186 6559 1563
Size: 3478 1145 373 3063
Fragments arranged by size:
3478 3063 1145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2004 2147 2373 2691 3279 3552 3945 4163
Size: 372 143 226 318 588 273 393 218
Cuts at: 4163 5283 6122 7506 7774 1632
Size: 1120 839 1384 268 1917
Fragments arranged by size:
1917 1384 1120 839 588 393 372 318
273 268 226 218 143
AfeI AGC'GCT
Cuts at: 4148 4148
Size: 8059
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7862
AflIII A'CryG_T
Cuts at: 1203 4244 4817 6958 7935 1203
Size: 3041 573 2141 977 1327
Fragments arranged by size:
3041 2141 1327 977 573
AgeI A'CCGG_T
Cuts at: 6890 6890
Size: 8059
AhdI GACnn_n'nnGTC
Cuts at: 6788 6788
Size: 8059
AleI CACnn'nnGTG
Cuts at: 1166 5184 1166
Size: 4018 4041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2167 1184
Size: 983 7076
AlwNI CAG_nnn'CTG
Cuts at: 3158 3410 5802 7374 3158
Size: 252 2392 1572 3843
Fragments arranged by size:
3843 2392 1572 252
ApaLI G'TGCA_C
Cuts at: 2114 6116 7272 2114
Size: 4002 1156 2901
Fragments arranged by size:
4002 2901 1156
ApoI r'AATT_y
Cuts at: 1493 3840 4456 4490 5368 5594 5746 7855 1493
Size: 2347 616 34 878 226 152 2109 1697
Fragments arranged by size:
2347 2109 1697 878 616 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 6613 160
Size: 872 5581 1606
Fragments arranged by size:
5581 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8059
AvaI C'yCGr_G
Cuts at: 1870 1943 5384 1870
Size: 73 3441 4545
Fragments arranged by size:
4545 3441 73
BamHI G'GATC_C
Cuts at: 5389 5756 5389
Size: 367 7692
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5380 6835 618
Size: 325 217 627 3593 1455 1842
Fragments arranged by size:
3593 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4975 5378 1391
Size: 3584 403 4072
Fragments arranged by size:
4072 3584 403
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 928
Size: 971 179 6909
Fragments arranged by size:
6909 971 179
BbvCI CC'TCA_GC
Cuts at: 3625 3625
Size: 8059
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6250 6284 1903
Size: 34 50 34 4229 34 3678
Fragments arranged by size:
4229 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3862 4586 5951 7161 3862
Size: 724 1365 1210 4760
Fragments arranged by size:
4760 1365 1210 724
BclI T'GATC_A
Cuts at: 1548 4699 5257 1548
Size: 3151 558 4350
Fragments arranged by size:
4350 3151 558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6670 136
Size: 107 122 71 631 605 4998 1525
Fragments arranged by size:
4998 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2130 4263 4651 8054 2130
Size: 2133 388 3403 2135
Fragments arranged by size:
3403 2135 2133 388
BlpI GC'TnA_GC
Cuts at: 4116 5476 4116
Size: 1360 6699
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2125 3771 6120 7276 1790
Size: 328 7 1646 2349 1156 2573
Fragments arranged by size:
2573 2349 1646 1156 328 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 5790 6748
Size: 416 53 83 399 24 1509 2864 958
Cuts at: 6748 442
Size: 1753
Fragments arranged by size:
2864 1753 1509 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3126 6719 1239
Size: 1887 3593 2579
Fragments arranged by size:
3593 2579 1887
Bpu10I CC'TnA_GC
Cuts at: 2949 3625 2949
Size: 676 7383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 4252 6184 7049 7347 2390
Size: 1862 1932 865 298 3102
Fragments arranged by size:
3102 1932 1862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 4646 6722 882
Size: 617 1026 2121 2076 2219
Fragments arranged by size:
2219 2121 2076 1026 617
BsaAI yAC'GTr
Cuts at: 493 1204 2203 5850 493
Size: 711 999 3647 2702
Fragments arranged by size:
3647 2702 999 711
BsaBI GATnn'nnATC
Cuts at: 5000 8053 5000
Size: 3053 5006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3056 5866 6248
Size: 53 83 186 936 1438 85 2810 382
Cuts at: 6248 6883 275
Size: 635 1451
Fragments arranged by size:
2810 1451 1438 936 635 382 186 85
83 53
BsaWI w'CCGG_w
Cuts at: 2358 2826 4045 6492 6890 7164 7311 7722 2358
Size: 468 1219 2447 398 274 147 411 2695
Fragments arranged by size:
2695 2447 1219 468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4198 4228 1178
Size: 30 165 30 2795 30 5009
Fragments arranged by size:
5009 2795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3297 4147 1186
Size: 2111 850 5098
Fragments arranged by size:
5098 2111 850
BseYI C'CCAG_C
Cuts at: 3966 7262 7758 3966
Size: 3296 496 4267
Fragments arranged by size:
4267 3296 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4050 1163
Size: 573 2314 5172
Fragments arranged by size:
5172 2314 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5509 6270 7298 1540
Size: 275 186 3508 761 1028 2301
Fragments arranged by size:
3508 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3922 4975 6120 6205 7276 2118
Size: 1804 1053 1145 85 1071 2901
Fragments arranged by size:
2901 1804 1145 1071 1053 85
BsmI GAATG_Cn'
Cuts at: 2156 2628 5570 5663 2156
Size: 472 2942 93 4552
Fragments arranged by size:
4552 2942 472 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2125 3771 3922 4975 5378 6120
Size: 399 328 7 1646 151 1053 403 742
Cuts at: 6120 6205 7276 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1646 1071 1053 742 403 399 328
151 85 7
BspEI T'CCGG_A
Cuts at: 2358 2826 7722 2358
Size: 468 4896 2695
Fragments arranged by size:
4896 2695 468
BspHI T'CATG_A
Cuts at: 4503 5260 5948 7678 4503
Size: 757 688 1730 4884
Fragments arranged by size:
4884 1730 757 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 3052 3070 5049 5414 844
Size: 1883 325 18 1979 365 3489
Fragments arranged by size:
3489 1979 1883 365 325 18
BsrBI CCG'CTC
Cuts at: 1394 1823 4120 5946 1394
Size: 429 2297 1826 3507
Fragments arranged by size:
3507 2297 1826 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2512 2884 4963 6554 6728 65
Size: 1183 1264 372 2079 1591 174 1396
Fragments arranged by size:
2079 1591 1396 1264 1183 372 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6703 6890 1700
Size: 110 4893 187 2869
Fragments arranged by size:
4893 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4889 96
Size: 4793 3266
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8059
BssSI C'ACGA_G
Cuts at: 5337 6119 7131 5337
Size: 782 1012 6265
Fragments arranged by size:
6265 1012 782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3410 4730 4965 794
Size: 2616 1320 235 3888
Fragments arranged by size:
3888 2616 1320 235
BstBI TT'CG_AA
Cuts at: 6863 6863
Size: 8059
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8059
BstYI r'GATC_y
Cuts at: 1972 2130 4263 4651 5389 5756 6141 6158 7610
Size: 158 2133 388 738 367 385 17 1452
Cuts at: 7610 7696 7708 8054 1972
Size: 86 12 346 1977
Fragments arranged by size:
2133 1977 1452 738 388 385 367 346
158 86 17 12
BstZ17I GTA'TAC
Cuts at: 2937 2937
Size: 8059
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7320
Fragments arranged by size:
7320 683 56
BtsI GCAGTG_nn'
Cuts at: 2694 4388 5575 6369 6389 2694
Size: 1694 1187 794 20 4364
Fragments arranged by size:
4364 1694 1187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8024
DraI TTT'AAA
Cuts at: 4555 4717 5363 4555
Size: 162 646 7251
Fragments arranged by size:
7251 646 162
DraIII CAC_nnn'GTG
Cuts at: 3926 3926
Size: 8059
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7066 809
Size: 507 5750 1802
Fragments arranged by size:
5750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5506 6395 8024
Size: 54 1425 50 275 18 3676 889 1629
Cuts at: 8024 8
Size: 43
Fragments arranged by size:
3676 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5506 1537
Size: 275 3694 4090
Fragments arranged by size:
4090 3694 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2425 2744 2976 3255 3420 4185 4190 4670
Size: 1072 319 232 279 165 765 5 480
Cuts at: 4670 5989 1353
Size: 1319 3423
Fragments arranged by size:
3423 1319 1072 765 480 319 279 232
165 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6646 7020 7166 387
Size: 1064 138 5057 374 146 1280
Fragments arranged by size:
5057 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4973 4973
Size: 8059
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2147 2373 2691 3126 3279 3552
Size: 393 372 143 226 318 435 153 273
Cuts at: 3552 3945 4163 5283 6122 6719 7506 7774 1239
Size: 393 218 1120 839 597 787 268 1524
Fragments arranged by size:
1524 1120 839 787 597 435 393 393
372 318 273 268 226 218 153 143
EcoO109I rG'GnC_Cy
Cuts at: 2178 2178
Size: 8059
EcoRI G'AATT_C
Cuts at: 3840 5368 5746 3840
Size: 1528 378 6153
Fragments arranged by size:
6153 1528 378
EcoRV GAT'ATC
Cuts at: 5275 5275
Size: 8059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2861 4637 2861
Size: 1776 6283
FspI TGC'GCA
Cuts at: 2876 6565 2876
Size: 3689 4370
HaeII r_GCGC'y
Cuts at: 1678 4150 7206 1678
Size: 2472 3056 2531
Fragments arranged by size:
3056 2531 2472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3088 3120 3287 3319 6700
Size: 32 135 32 1516 32 167 32 3381
Cuts at: 6700 6732 6774 6806 1373
Size: 32 42 32 2626
Fragments arranged by size:
3381 2626 1516 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3756 5403 5649 669
Size: 988 281 1437 381 1647 246 3079
Fragments arranged by size:
3079 1647 1437 988 381 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8059
HpaI GTT'AAC
Cuts at: 3375 5649 3375
Size: 2274 5785
KpnI G_GTAC'C
Cuts at: 5384 5384
Size: 8059
MfeI C'AATT_G
Cuts at: 5658 5658
Size: 8059
MluI A'CGCG_T
Cuts at: 7935 7935
Size: 8059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 3386 3755 4109 4241 7173 7357 1305
Size: 1379 702 369 354 132 2932 184 2007
Fragments arranged by size:
2932 2007 1379 702 369 354 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6237
Fragments arranged by size:
6237 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 4473 4707 5184 6017 6376 6535
Size: 648 1410 1897 234 477 833 359 159
Cuts at: 6535 518
Size: 2042
Fragments arranged by size:
2042 1897 1410 833 648 477 359 234
159
MspA1I CmG'CkG
Cuts at: 1606 2401 5475 6152 7300 7545 1606
Size: 795 3074 677 1148 245 2120
Fragments arranged by size:
3074 2120 1148 795 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7507
NdeI CA'TA_TG
Cuts at: 387 4035 387
Size: 3648 4411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8059
NotI GC'GGCC_GC
Cuts at: 5506 5506
Size: 8059
NspI r_CATG'y
Cuts at: 1132 1229 4248 4733 4821 5417 6962 1132
Size: 97 3019 485 88 596 1545 2229
Fragments arranged by size:
3019 2229 1545 596 485 97 88
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8059
PciI A'CATG_T
Cuts at: 4244 4817 6958 4244
Size: 573 2141 5345
Fragments arranged by size:
5345 2141 573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3836 3992 1108
Size: 2728 156 5175
Fragments arranged by size:
5175 2728 156
PfoI T'CCnGG_A
Cuts at: 2686 2686
Size: 8059
PmeI GTTT'AAAC
Cuts at: 5363 5363
Size: 8059
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2025 2057 6128 6160 7641 7673 2025
Size: 32 4071 32 1481 32 2411
Fragments arranged by size:
4071 2411 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8059
PsiI TTA'TAA
Cuts at: 2214 2606 5629 2214
Size: 392 3023 4644
Fragments arranged by size:
4644 3023 392
PstI C_TGCA'G
Cuts at: 830 4726 4961 5411 830
Size: 3896 235 450 3478
Fragments arranged by size:
3896 3478 450 235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8059
SacI G_AGCT'C
Cuts at: 4975 4975
Size: 8059
SalI G'TCGA_C
Cuts at: 1936 5401 1936
Size: 3465 4594
SapI GCTCTTCn'nnn_
Cuts at: 3255 3420 3255
Size: 165 7894
SbfI CC_TGCA'GG
Cuts at: 5411 5411
Size: 8059
ScaI AGT'ACT
Cuts at: 6307 8019 6307
Size: 1712 6347
SfcI C'TryA_G
Cuts at: 826 1045 3069 3705 4722 4800 4957 5407 6542
Size: 219 2024 636 1017 78 157 450 1135
Cuts at: 6542 7223 7414 826
Size: 681 191 1471
Fragments arranged by size:
2024 1471 1135 1017 681 636 450 219
191 157 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8059
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8059
SmaI CCC'GGG
Cuts at: 1872 5386 1872
Size: 3514 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 4267 6163 7064 7326 820
Size: 197 926 426 1898 1896 901 262 1553
Fragments arranged by size:
1898 1896 1553 926 901 426 262 197
SnaBI TAC'GTA
Cuts at: 493 5850 493
Size: 5357 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8059
SphI G_CATG'C
Cuts at: 1132 5417 1132
Size: 4285 3774
SspI AAT'ATT
Cuts at: 5 52 4377 5983 5
Size: 47 4325 1606 2081
Fragments arranged by size:
4325 2081 1606 47
StuI AGG'CCT
Cuts at: 1517 4847 1517
Size: 3330 4729
StyI C'CwwG_G
Cuts at: 513 1065 5498 513
Size: 552 4433 3074
Fragments arranged by size:
4433 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3196 4033 6086 6103 6256 6441 6886
Size: 1255 1616 837 2053 17 153 185 445
Cuts at: 6886 325
Size: 1498
Fragments arranged by size:
2053 1616 1498 1255 837 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2185 3284 4804
Size: 274 80 33 51 1443 208 1099 1520
Cuts at: 4804 4889 6305 8017 96
Size: 85 1416 1712 138
Fragments arranged by size:
1712 1520 1443 1416 1099 274 208 138
85 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8019 8051 8019
Size: 32 8027
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8059
XbaI T'CTAG_A
Cuts at: 5395 5395
Size: 8059
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2270 2504 2011
Size: 259 234 7566
Fragments arranged by size:
7566 259 234
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8059
XmaI C'CCGG_G
Cuts at: 1870 5384 1870
Size: 3514 4545
XmnI GAAnn'nnTTC
Cuts at: 3030 4620 6188 3030
Size: 1590 1568 4901
Fragments arranged by size:
4901 1590 1568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5867 276
Size: 53 83 186 936 4333 2468
Fragments arranged by size:
4333 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI Bsu36I EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI