Restriction map for
pFN21AA0051
(Circular) (Six-base) MAPSORT of: pFN21AA0051.seq Check: 6607 from: 1 to: 9676
pFN21AA0051 9676 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4352 4635 6562 4352
Size: 283 1927 7466
Fragments arranged by size:
7466 1927 283
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2113 7486 278
Size: 53 83 186 936 577 5373 2468
Fragments arranged by size:
5373 2468 936 577 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 7019 1937
Size: 295 4787 4594
Fragments arranged by size:
4787 4594 295
Acc65I G'GTAC_C
Cuts at: 6486 6997 6486
Size: 511 9165
AclI AA'CG_TT
Cuts at: 1563 6172 7803 8176 1563
Size: 4609 1631 373 3063
Fragments arranged by size:
4609 3063 1631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3536 4032 4340 5525 5576 5684 5813 7739
Size: 1904 496 308 1185 51 108 129 1926
Cuts at: 7739 9123 9391 1632
Size: 1384 268 1917
Fragments arranged by size:
1926 1917 1904 1384 1185 496 308 268
129 108 51
AfeI AGC'GCT
Cuts at: 4475 4475
Size: 9676
AflII C'TTAA_G
Cuts at: 820 1017 5746 820
Size: 197 4729 4750
Fragments arranged by size:
4750 4729 197
AflIII A'CryG_T
Cuts at: 1203 5040 8575 9552 1203
Size: 3837 3535 977 1327
Fragments arranged by size:
3837 3535 1327 977
AgeI A'CCGG_T
Cuts at: 8507 8507
Size: 9676
AhdI GACnn_n'nnGTC
Cuts at: 8405 8405
Size: 9676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2606 6300 1184
Size: 1422 3694 4560
Fragments arranged by size:
4560 3694 1422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4269 4301 4269
Size: 32 9644
AlwNI CAG_nnn'CTG
Cuts at: 3055 3455 4447 4720 4927 6445 6868 7419 8991
Size: 400 992 273 207 1518 423 551 1572
Cuts at: 8991 3055
Size: 3740
Fragments arranged by size:
3740 1572 1518 992 551 423 400 273
207
ApaLI G'TGCA_C
Cuts at: 7733 8889 7733
Size: 1156 8520
ApoI r'AATT_y
Cuts at: 1493 2697 2889 3545 3819 4138 4150 4295 4312
Size: 1204 192 656 274 319 12 145 17
Cuts at: 4312 4616 5786 5837 6821 6985 7211 7363 9472
Size: 304 1170 51 984 164 226 152 2109
Cuts at: 9472 1493
Size: 1697
Fragments arranged by size:
2109 1697 1204 1170 984 656 319 304
274 226 192 164 152 145 51 17
12
AseI AT'TA_AT
Cuts at: 160 1032 2676 3189 3639 3894 5757 8230 160
Size: 872 1644 513 450 255 1863 2473 1606
Fragments arranged by size:
2473 1863 1644 1606 872 513 450 255
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9676
AvaI C'yCGr_G
Cuts at: 1870 1943 4167 4557 7001 1870
Size: 73 2224 390 2444 4545
Fragments arranged by size:
4545 2444 2224 390 73
AvrII C'CTAG_G
Cuts at: 2176 6647 2176
Size: 4471 5205
BamHI G'GATC_C
Cuts at: 4591 7006 7373 4591
Size: 2415 367 6894
Fragments arranged by size:
6894 2415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5186 6004 6486 6997 8452
Size: 325 217 627 3399 818 482 511 1455
Cuts at: 8452 618
Size: 1842
Fragments arranged by size:
3399 1842 1455 818 627 511 482 325
217
BanII G_rGCy'C
Cuts at: 1391 2763 2986 3354 5684 6995 1391
Size: 1372 223 368 2330 1311 4072
Fragments arranged by size:
4072 2330 1372 1311 368 223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2567 3227 5393 6348 928
Size: 971 668 660 2166 955 4256
Fragments arranged by size:
4256 2166 971 955 668 660
BbvCI CC'TCA_GC
Cuts at: 4587 4587
Size: 9676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2696 2722 2730 2756 4148 4182 4448
Size: 34 759 26 8 26 1392 34 266
Cuts at: 4448 4482 7867 7901 1903
Size: 34 3385 34 3678
Fragments arranged by size:
3678 3385 1392 759 266 34 34 34
34 26 26 8
BciVI GTATCCnnnnn_n'
Cuts at: 2468 3279 4271 4361 7568 8778 2468
Size: 811 992 90 3207 1210 3366
Fragments arranged by size:
3366 3207 1210 992 811 90
BclI T'GATC_A
Cuts at: 1548 3390 4248 6447 1548
Size: 1842 858 2199 4777
Fragments arranged by size:
4777 2199 1842 858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8287 136
Size: 107 122 71 631 605 6615 1525
Fragments arranged by size:
6615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2185 5214 9671 2185
Size: 3029 4457 2190
Fragments arranged by size:
4457 3029 2190
BlpI GC'TnA_GC
Cuts at: 2799 7093 2799
Size: 4294 5382
Bme1580I G_kGCm'C
Cuts at: 1790 3940 5191 6009 7737 8893 1790
Size: 2150 1251 818 1728 1156 2573
Fragments arranged by size:
2573 2150 1728 1251 1156 818
BmgBI CAC'GTC
Cuts at: 1318 6115 6284 1318
Size: 4797 169 4710
Fragments arranged by size:
4797 4710 169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 4078 5219
Size: 416 53 83 399 24 838 1823 1141
Cuts at: 5219 5767 7407 8365 442
Size: 548 1640 958 1753
Fragments arranged by size:
1823 1753 1640 1141 958 838 548 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5372 1056
Size: 4316 5360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 3144 4124 4742 6141 8336 1239
Size: 989 916 980 618 1399 2195 2579
Fragments arranged by size:
2579 2195 1399 989 980 916 618
Bpu10I CC'TnA_GC
Cuts at: 2219 3522 4587 5358 2219
Size: 1303 1065 771 6537
Fragments arranged by size:
6537 1303 1065 771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3728 4862 7801 8666 8964 3728
Size: 1134 2939 865 298 4440
Fragments arranged by size:
4440 2939 1134 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 4880 6203 8339 882
Size: 617 2601 780 1323 2136 2219
Fragments arranged by size:
2601 2219 2136 1323 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 7467 493
Size: 711 6263 2702
Fragments arranged by size:
6263 2702 711
BsaBI GATnn'nnATC
Cuts at: 3288 9670 3288
Size: 6382 3294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2110 7483 7865 8500
Size: 53 83 186 936 577 5373 382 635
Cuts at: 8500 275
Size: 1451
Fragments arranged by size:
5373 1451 936 635 577 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5956 6192 8109 8507 8781 8928 9339 5956
Size: 236 1917 398 274 147 411 6293
Fragments arranged by size:
6293 1917 411 398 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3104 3134 3359 3389 4397
Size: 30 165 30 1701 30 225 30 1008
Cuts at: 4397 4427 6882 6912 1178
Size: 30 2455 30 3942
Fragments arranged by size:
3942 2455 1701 1008 225 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2225 2882 3125 4187 4673 5072 6511
Size: 919 120 657 243 1062 486 399 1439
Cuts at: 6511 1186
Size: 4351
Fragments arranged by size:
4351 1439 1062 919 657 486 399 243
120
BseYI C'CCAG_C
Cuts at: 2032 3150 3252 3732 8879 9375 2032
Size: 1118 102 480 5147 496 2333
Fragments arranged by size:
5147 2333 1118 496 480 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 9103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 7126 7887 8915 1540
Size: 275 186 47 5078 761 1028 2301
Fragments arranged by size:
5078 2301 1028 761 275 186 47
BsiHKAI G_wGCw'C
Cuts at: 3354 5964 7737 7822 8893 3354
Size: 2610 1773 85 1071 4137
Fragments arranged by size:
4137 2610 1773 1071 85
BsmI GAATG_Cn'
Cuts at: 2371 2740 4347 5206 6136 7187 7280 2371
Size: 369 1607 859 930 1051 93 4767
Fragments arranged by size:
4767 1607 1051 930 859 369 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2101 6274 2101
Size: 4173 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2763 2986 3354 3940 5191 5684 5964
Size: 399 973 223 368 586 1251 493 280
Cuts at: 5964 6009 6995 7737 7822 8893 1391
Size: 45 986 742 85 1071 2174
Fragments arranged by size:
2174 1251 1071 986 973 742 586 493
399 368 280 223 85 45
BspEI T'CCGG_A
Cuts at: 5956 9339 5956
Size: 3383 6293
BspHI T'CATG_A
Cuts at: 5374 6900 7565 9295 5374
Size: 1526 665 1730 5755
Fragments arranged by size:
5755 1730 1526 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4352 4416 4635 5073 6562 6611 6750 6876
Size: 3508 64 219 438 1489 49 139 126
Cuts at: 6876 7031 844
Size: 155 3489
Fragments arranged by size:
3508 3489 1489 438 219 155 139 126
64 49
BsrBI CCG'CTC
Cuts at: 1394 1823 7563 1394
Size: 429 5740 3507
Fragments arranged by size:
5740 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5490 5685 5949 6456 8171 8345 65
Size: 1183 4242 195 264 507 1715 174 1396
Fragments arranged by size:
4242 1715 1396 1183 507 264 195 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8320 8507 1700
Size: 110 6510 187 2869
Fragments arranged by size:
6510 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9676
BssSI C'ACGA_G
Cuts at: 7736 8748 7736
Size: 1012 8664
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2657 3143 794
Size: 1863 486 7327
Fragments arranged by size:
7327 1863 486
BstBI TT'CG_AA
Cuts at: 6807 8480 6807
Size: 1673 8003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9676
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 4960 5008 6826 2206
Size: 2754 48 1818 5056
Fragments arranged by size:
5056 2754 1818 48
BstYI r'GATC_y
Cuts at: 1972 2185 4176 4591 5214 6378 7006 7373 7758
Size: 213 1991 415 623 1164 628 367 385
Cuts at: 7758 7775 9227 9313 9325 9671 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1991 1977 1452 1164 628 623 415 385
367 346 213 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3877 3877
Size: 9676
BtgI C'CryG_G
Cuts at: 513 1065 2370 4273 5182 5191 6417 513
Size: 552 1305 1903 909 9 1226 3772
Fragments arranged by size:
3772 1903 1305 1226 909 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8937
Fragments arranged by size:
8937 683 56
BtsI GCAGTG_nn'
Cuts at: 3369 7192 7986 8006 3369
Size: 3823 794 20 5039
Fragments arranged by size:
5039 3823 794 20
ClaI AT'CG_AT
Cuts at: 1554 5584 1554
Size: 4030 5646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9641
DraI TTT'AAA
Cuts at: 6048 6393 6760 6980 6048
Size: 345 367 220 8744
Fragments arranged by size:
8744 367 345 220
DraIII CAC_nnn'GTG
Cuts at: 3935 3935
Size: 9676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3084 6085 8683 809
Size: 507 1768 3001 2598 1802
Fragments arranged by size:
3001 2598 1802 1768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 4233 4962
Size: 54 1425 50 275 18 215 2188 729
Cuts at: 4962 5194 7123 8012 9641 8
Size: 232 1929 889 1629 43
Fragments arranged by size:
2188 1929 1629 1425 889 729 275 232
215 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 7123 1537
Size: 275 233 5078 4090
Fragments arranged by size:
5078 4090 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2751 3068 3758 4680 4892 6363 7606
Size: 797 601 317 690 922 212 1471 1243
Cuts at: 7606 1353
Size: 3423
Fragments arranged by size:
3423 1471 1243 922 797 690 601 317
212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 1999 8263 8637 8783 387
Size: 1064 138 410 6264 374 146 1280
Fragments arranged by size:
6264 1280 1064 410 374 146 138
EcoICRI GAG'CTC
Cuts at: 3352 3352
Size: 9676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 3144 3536 4032 4124 4340 4742
Size: 393 596 916 392 496 92 216 402
Cuts at: 4742 5525 5576 5684 5813 6141 7739 8336 9123
Size: 783 51 108 129 328 1598 597 787
Cuts at: 9123 9391 1239
Size: 268 1524
Fragments arranged by size:
1598 1524 916 787 783 597 596 496
402 393 392 328 268 216 129 108
92 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2977 3104 5999 2977
Size: 127 2895 6654
Fragments arranged by size:
6654 2895 127
EcoO109I rG'GnC_Cy
Cuts at: 3682 6739 6865 3682
Size: 3057 126 6493
Fragments arranged by size:
6493 3057 126
EcoRI G'AATT_C
Cuts at: 2697 3545 4295 6985 7363 2697
Size: 848 750 2690 378 5010
Fragments arranged by size:
5010 2690 848 750 378
EcoRV GAT'ATC
Cuts at: 2419 3565 4747 2419
Size: 1146 1182 7348
Fragments arranged by size:
7348 1182 1146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 3892 6574 6740 2548
Size: 1344 2682 166 5484
Fragments arranged by size:
5484 2682 1344 166
FspI TGC'GCA
Cuts at: 8182 8182
Size: 9676
HaeII r_GCGC'y
Cuts at: 1678 4477 5369 8823 1678
Size: 2799 892 3454 2531
Fragments arranged by size:
3454 2799 2531 892
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3104 3136 3359 3391 5131
Size: 32 135 32 1532 32 223 32 1740
Cuts at: 5131 5163 5237 5269 5299 5331 5840 5872 6273
Size: 32 74 32 30 32 509 32 401
Cuts at: 6273 6305 6882 6914 8317 8349 8391 8423 1373
Size: 32 577 32 1403 32 42 32 2626
Fragments arranged by size:
2626 1740 1532 1403 577 509 401 223
135 74 42 32 32 32 32 32
32 32 32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 2026 2530 6934 7020 7266 669
Size: 988 281 88 504 4404 86 246 3079
Fragments arranged by size:
4404 3079 988 504 281 246 88 86
HindIII A'AGCT_T
Cuts at: 748 3570 4914 748
Size: 2822 1344 5510
Fragments arranged by size:
5510 2822 1344
HpaI GTT'AAC
Cuts at: 7266 7266
Size: 9676
KpnI G_GTAC'C
Cuts at: 6490 7001 6490
Size: 511 9165
MfeI C'AATT_G
Cuts at: 3437 7275 3437
Size: 3838 5838
MluI A'CGCG_T
Cuts at: 5040 9552 5040
Size: 4512 5164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 3792 3867 4890 5070 5913 6778 6809
Size: 1245 1242 75 1023 180 843 865 31
Cuts at: 6809 8790 8974 1305
Size: 1981 184 2007
Fragments arranged by size:
2007 1981 1245 1242 1023 865 843 184
180 75 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4235 4964 5196 10
Size: 54 1425 343 2403 729 232 4490
Fragments arranged by size:
4490 2403 1425 729 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2588 4422 4512 5943 7634 7993 8152
Size: 648 1422 1834 90 1431 1691 359 159
Cuts at: 8152 518
Size: 2042
Fragments arranged by size:
2042 1834 1691 1431 1422 648 359 159
90
MspA1I CmG'CkG
Cuts at: 1606 3256 3800 4225 4720 6481 7092 7769 8917
Size: 1650 544 425 495 1761 611 677 1148
Cuts at: 8917 9162 1606
Size: 245 2120
Fragments arranged by size:
2120 1761 1650 1148 677 611 544 495
425 245
NcoI C'CATG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 7819
Fragments arranged by size:
7819 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9676
NheI G'CTAG_C
Cuts at: 1052 5368 1052
Size: 4316 5360
NotI GC'GGCC_GC
Cuts at: 7123 7123
Size: 9676
NspI r_CATG'y
Cuts at: 1132 1229 2175 2440 2660 3533 5419 5708 7034
Size: 97 946 265 220 873 1886 289 1326
Cuts at: 7034 8579 1132
Size: 1545 2229
Fragments arranged by size:
2229 1886 1545 1326 946 873 289 265
220 97
PacI TTA_AT'TAA
Cuts at: 3639 3639
Size: 9676
PasI CC'CwG_GG
Cuts at: 1348 3001 1348
Size: 1653 8023
PciI A'CATG_T
Cuts at: 8575 8575
Size: 9676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4102 4630 4720 1108
Size: 2994 528 90 6064
Fragments arranged by size:
6064 2994 528 90
PfoI T'CCnGG_A
Cuts at: 2064 3786 4304 4500 2064
Size: 1722 518 196 7240
Fragments arranged by size:
7240 1722 518 196
PmeI GTTT'AAAC
Cuts at: 6980 6980
Size: 9676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 3321 3353 7745 7777 9258 9290 2243
Size: 32 1046 32 4392 32 1481 32 2629
Fragments arranged by size:
4392 2629 1481 1046 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6739 6865 6739
Size: 126 9550
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9676
PsiI TTA'TAA
Cuts at: 7246 7246
Size: 9676
PstI C_TGCA'G
Cuts at: 830 2089 2991 3021 3105 3120 3315 3681 3700
Size: 1259 902 30 84 15 195 366 19
Cuts at: 3700 4263 4494 6876 7028 830
Size: 563 231 2382 152 3478
Fragments arranged by size:
3478 2382 1259 902 563 366 231 195
152 84 30 19 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9676
PvuII CAG'CTG
Cuts at: 3256 3800 4225 4720 3256
Size: 544 425 495 8212
Fragments arranged by size:
8212 544 495 425
SacI G_AGCT'C
Cuts at: 3354 3354
Size: 9676
SalI G'TCGA_C
Cuts at: 1936 7018 1936
Size: 5082 4594
SapI GCTCTTCn'nnn_
Cuts at: 2751 2751
Size: 9676
SbfI CC_TGCA'GG
Cuts at: 7028 7028
Size: 9676
ScaI AGT'ACT
Cuts at: 4496 5855 7924 9636 4496
Size: 1359 2069 1712 4536
Fragments arranged by size:
4536 2069 1712 1359
SexAI A'CCwGG_T
Cuts at: 3591 3591
Size: 9676
SfcI C'TryA_G
Cuts at: 826 1045 2085 2412 2987 3017 3101 3116 3311
Size: 219 1040 327 575 30 84 15 195
Cuts at: 3311 3677 3686 3696 4259 4490 6872 6878 7024
Size: 366 9 10 563 231 2382 6 146
Cuts at: 7024 8159 8840 9031 826
Size: 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 1040 681 575 563 366
327 231 219 195 191 146 84 30
15 10 9 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9676
SmaI CCC'GGG
Cuts at: 1872 7003 1872
Size: 5131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3707 4877 5746 7780 8681 8943
Size: 197 926 1764 1170 869 2034 901 262
Cuts at: 8943 820
Size: 1553
Fragments arranged by size:
2034 1764 1553 1170 926 901 869 262
197
SnaBI TAC'GTA
Cuts at: 493 7467 493
Size: 6974 2702
SpeI A'CTAG_T
Cuts at: 152 6511 152
Size: 6359 3317
SphI G_CATG'C
Cuts at: 1132 2175 5708 7034 1132
Size: 1043 3533 1326 3774
Fragments arranged by size:
3774 3533 1326 1043
SspI AAT'ATT
Cuts at: 5 52 3469 7600 5
Size: 47 3417 4131 2081
Fragments arranged by size:
4131 3417 2081 47
StuI AGG'CCT
Cuts at: 1517 2493 4245 1517
Size: 976 1752 6948
Fragments arranged by size:
6948 1752 976
StyI C'CwwG_G
Cuts at: 513 1065 2176 2370 2763 6647 7115 513
Size: 552 1111 194 393 3884 468 3074
Fragments arranged by size:
3884 3074 1111 552 468 393 194
SwaI ATTT'AAAT
Cuts at: 6048 6048
Size: 9676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3360 3869 4048 5933 7703 7720
Size: 1255 1776 4 509 179 1885 1770 17
Cuts at: 7720 7873 8058 8503 325
Size: 153 185 445 1498
Fragments arranged by size:
1885 1776 1770 1498 1255 509 445 185
179 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4494 5853 7922
Size: 274 80 33 51 1443 2517 1359 2069
Cuts at: 7922 9634 96
Size: 1712 138
Fragments arranged by size:
2517 2069 1712 1443 1359 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4749 4781 9636 9668 4749
Size: 32 4855 32 4757
Fragments arranged by size:
4855 4757 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3321 1316
Size: 2005 7671
XbaI T'CTAG_A
Cuts at: 7012 7012
Size: 9676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 5005 2203
Size: 2802 6874
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9676
XmaI C'CCGG_G
Cuts at: 1870 7001 1870
Size: 5131 4545
XmnI GAAnn'nnTTC
Cuts at: 2255 7805 2255
Size: 5550 4126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2111 7484 276
Size: 53 83 186 936 577 5373 2468
Fragments arranged by size:
5373 2468 936 577 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI