Restriction map for
pFN21AA0042
(Circular) (Six-base) MAPSORT of: pFN21AA0042.seq Check: 2877 from: 1 to: 9649
pFN21AA0042 9649 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7459 278
Size: 53 83 186 936 5923 2468
Fragments arranged by size:
5923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6992 1937
Size: 5055 4594
Acc65I G'GTAC_C
Cuts at: 6970 6970
Size: 9649
AclI AA'CG_TT
Cuts at: 1563 7776 8149 1563
Size: 6213 373 3063
Fragments arranged by size:
6213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2706 2936 5532 6305 6634 6777 7712
Size: 626 448 230 2596 773 329 143 935
Cuts at: 7712 9096 9364 1632
Size: 1384 268 1917
Fragments arranged by size:
2596 1917 1384 935 773 626 448 329
268 230 143
AfeI AGC'GCT
Cuts at: 3830 3830
Size: 9649
AflII C'TTAA_G
Cuts at: 820 1017 2111 2639 2891 4255 820
Size: 197 1094 528 252 1364 6214
Fragments arranged by size:
6214 1364 1094 528 252 197
AflIII A'CryG_T
Cuts at: 1203 2354 3094 8548 9525 1203
Size: 1151 740 5454 977 1327
Fragments arranged by size:
5454 1327 1151 977 740
AgeI A'CCGG_T
Cuts at: 8480 8480
Size: 9649
AhdI GACnn_n'nnGTC
Cuts at: 8378 8378
Size: 9649
AleI CACnn'nnGTG
Cuts at: 1166 3731 1166
Size: 2565 7084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5202 1184
Size: 4018 5631
AlwNI CAG_nnn'CTG
Cuts at: 2390 4233 7392 8964 2390
Size: 1843 3159 1572 3075
Fragments arranged by size:
3159 3075 1843 1572
ApaLI G'TGCA_C
Cuts at: 7706 8862 7706
Size: 1156 8493
ApoI r'AATT_y
Cuts at: 1493 2810 3507 3755 4009 4564 4778 5151 5202
Size: 1317 697 248 254 555 214 373 51
Cuts at: 5202 5598 5641 5698 5724 5823 6239 6509 6958
Size: 396 43 57 26 99 416 270 449
Cuts at: 6958 7184 7336 9445 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1317 697 555 449 416 396
373 270 254 248 226 214 152 99
57 51 43 26
AseI AT'TA_AT
Cuts at: 160 1032 3882 5808 8203 160
Size: 872 2850 1926 2395 1606
Fragments arranged by size:
2850 2395 1926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9649
AvaI C'yCGr_G
Cuts at: 1870 1943 6974 1870
Size: 73 5031 4545
Fragments arranged by size:
5031 4545 73
BamHI G'GATC_C
Cuts at: 6979 7346 6979
Size: 367 9282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6970 8425 618
Size: 325 217 627 5183 1455 1842
Fragments arranged by size:
5183 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 5264 6888 6914 6968 1391
Size: 1509 2364 1624 26 54 4072
Fragments arranged by size:
4072 2364 1624 1509 54 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 3139 5133 928
Size: 971 435 805 1994 5444
Fragments arranged by size:
5444 1994 971 805 435
BbvCI CC'TCA_GC
Cuts at: 2886 2886
Size: 9649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7840 7874 1903
Size: 34 5903 34 3678
Fragments arranged by size:
5903 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7541 8751 7541
Size: 1210 8439
BclI T'GATC_A
Cuts at: 1548 4546 1548
Size: 2998 6651
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8260 136
Size: 107 122 71 631 605 6588 1525
Fragments arranged by size:
6588 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2168 3421 3811 6398 9644 2168
Size: 1253 390 2587 3246 2173
Fragments arranged by size:
3246 2587 2173 1253 390
BlpI GC'TnA_GC
Cuts at: 4899 7066 4899
Size: 2167 7482
Bme1580I G_kGCm'C
Cuts at: 1790 7710 8866 1790
Size: 5920 1156 2573
Fragments arranged by size:
5920 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2357 1318
Size: 1039 8610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2607 4586 6056
Size: 416 53 83 399 24 1190 1979 1470
Cuts at: 6056 6924 7380 8338 442
Size: 868 456 958 1753
Fragments arranged by size:
1979 1753 1470 1190 958 868 456 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3261 3287 1056
Size: 2205 26 7418
Fragments arranged by size:
7418 2205 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 3850 2919
Size: 931 8718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5437 6489 6581 8309 1239
Size: 4198 1052 92 1728 2579
Fragments arranged by size:
4198 2579 1728 1052 92
Bpu10I CC'TnA_GC
Cuts at: 2886 6271 2886
Size: 3385 6264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 3417 4489 6035 7774 8639 8937 2632
Size: 785 1072 1546 1739 865 298 3344
Fragments arranged by size:
3344 1739 1546 1072 865 785 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8312 882
Size: 617 6813 2219
Fragments arranged by size:
6813 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7440 493
Size: 711 6236 2702
Fragments arranged by size:
6236 2702 711
BsaBI GATnn'nnATC
Cuts at: 2553 6588 9643 2553
Size: 4035 3055 2559
Fragments arranged by size:
4035 3055 2559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7456 7838 8473 275
Size: 53 83 186 936 5923 382 635 1451
Fragments arranged by size:
5923 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8082 8480 8754 8901 9312 8082
Size: 398 274 147 411 8419
Fragments arranged by size:
8419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5563 5593 1178
Size: 30 165 30 4160 30 5234
Fragments arranged by size:
5234 4160 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 5584 6728 1186
Size: 1107 3291 1144 4107
Fragments arranged by size:
4107 3291 1144 1107
BseYI C'CCAG_C
Cuts at: 2899 6901 8852 9348 2899
Size: 4002 1951 496 3200
Fragments arranged by size:
4002 3200 1951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2896 3903 5267 6546 1163
Size: 573 1160 1007 1364 1279 4266
Fragments arranged by size:
4266 1364 1279 1160 1007 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7099 7860 8888 1540
Size: 275 186 5098 761 1028 2301
Fragments arranged by size:
5098 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5264 6505 6888 6914 7710 7795 8866 5264
Size: 1241 383 26 796 85 1071 6047
Fragments arranged by size:
6047 1241 1071 796 383 85 26
BsmI GAATG_Cn'
Cuts at: 2092 5647 6127 6926 7160 7253 2092
Size: 3555 480 799 234 93 4488
Fragments arranged by size:
4488 3555 799 480 234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2900 5264 6505 6888 6914 6968 7710
Size: 399 1110 2364 1241 383 26 54 742
Cuts at: 7710 7795 8866 1391
Size: 85 1071 2174
Fragments arranged by size:
2364 2174 1241 1110 1071 742 399 383
85 54 26
BspEI T'CCGG_A
Cuts at: 9312 9312
Size: 9649
BspHI T'CATG_A
Cuts at: 3150 5857 7538 9268 3150
Size: 2707 1681 1730 3531
Fragments arranged by size:
3531 2707 1730 1681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2165 7004 844
Size: 1321 4839 3489
Fragments arranged by size:
4839 3489 1321
BsrBI CCG'CTC
Cuts at: 1394 1823 7536 1394
Size: 429 5713 3507
Fragments arranged by size:
5713 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 4029 4113 5457 6526 8144 8318
Size: 1183 1416 1365 84 1344 1069 1618 174
Cuts at: 8318 65
Size: 1396
Fragments arranged by size:
1618 1416 1396 1365 1344 1183 1069 174
84
BsrFI r'CCGG_y
Cuts at: 1700 1810 8293 8480 1700
Size: 110 6483 187 2869
Fragments arranged by size:
6483 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2846 6136 6344 96
Size: 2750 3290 208 3401
Fragments arranged by size:
3401 3290 2750 208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9649
BssSI C'ACGA_G
Cuts at: 7709 8721 7709
Size: 1012 8637
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4028 4172 794
Size: 3234 144 6271
Fragments arranged by size:
6271 3234 144
BstBI TT'CG_AA
Cuts at: 3312 8453 3312
Size: 5141 4508
BstEII G'GTnAC_C
Cuts at: 1184 6358 1184
Size: 5174 4475
BstYI r'GATC_y
Cuts at: 1972 2168 2554 2987 3421 3811 4429 6398 6979
Size: 196 386 433 434 390 618 1969 581
Cuts at: 6979 7346 7731 7748 9200 9286 9298 9644 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1969 1452 618 581 434 433 390
386 385 367 346 196 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 6384 6889 513
Size: 552 5319 505 3273
Fragments arranged by size:
5319 3273 552 505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8910
Fragments arranged by size:
8910 683 56
BtsI GCAGTG_nn'
Cuts at: 3829 6347 6912 7165 7959 7979 3829
Size: 2518 565 253 794 20 5499
Fragments arranged by size:
5499 2518 794 565 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4289 4324 529
Size: 35 3725 35 5854
Fragments arranged by size:
5854 3725 35 35
DraI TTT'AAA
Cuts at: 6953 6953
Size: 9649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5624 8656 809
Size: 507 4308 3032 1802
Fragments arranged by size:
4308 3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6627 7096 7985
Size: 54 1425 50 275 18 4797 469 889
Cuts at: 7985 9614 8
Size: 1629 43
Fragments arranged by size:
4797 1629 1425 889 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7096 1537
Size: 275 5284 4090
Fragments arranged by size:
5284 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2539 4735 4965 7579 1353
Size: 1186 2196 230 2614 3423
Fragments arranged by size:
3423 2614 2196 1186 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5129 8236 8610 8756 387
Size: 1064 138 3540 3107 374 146 1280
Fragments arranged by size:
3540 3107 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5262 6886 6912 5262
Size: 1624 26 7999
Fragments arranged by size:
7999 1624 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2706 2936 5437 5532 6305 6489
Size: 393 626 448 230 2501 95 773 184
Cuts at: 6489 6581 6634 6777 7712 8309 9096 9364 1239
Size: 92 53 143 935 597 787 268 1524
Fragments arranged by size:
2501 1524 935 787 773 626 597 448
393 268 230 184 143 95 92 53
EcoRI G'AATT_C
Cuts at: 5151 6239 6509 6958 7336 5151
Size: 1088 270 449 378 7464
Fragments arranged by size:
7464 1088 449 378 270
EcoRV GAT'ATC
Cuts at: 2740 3637 2740
Size: 897 8752
FspI TGC'GCA
Cuts at: 8155 8155
Size: 9649
HaeII r_GCGC'y
Cuts at: 1678 3832 8796 1678
Size: 2154 4964 2531
Fragments arranged by size:
4964 2531 2154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3559 3591 4186 4215 4218
Size: 32 135 32 1987 32 595 29 3
Cuts at: 4218 4247 6080 6112 6385 6417 6481 6513 8290
Size: 29 1833 32 273 32 64 32 1777
Cuts at: 8290 8322 8364 8396 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1987 1833 1777 595 273 135 64
42 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4111 5077 6993 7239 669
Size: 988 281 2173 966 1916 246 3079
Fragments arranged by size:
3079 2173 1916 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 5160 748
Size: 4412 5237
HpaI GTT'AAC
Cuts at: 7239 7239
Size: 9649
KpnI G_GTAC'C
Cuts at: 6974 6974
Size: 9649
MfeI C'AATT_G
Cuts at: 7248 7248
Size: 9649
MluI A'CGCG_T
Cuts at: 3094 9525 3094
Size: 6431 3218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2109 2304 2715 4184 4465 8763 8947 1305
Size: 804 195 411 1469 281 4298 184 2007
Fragments arranged by size:
4298 2007 1469 804 411 281 195 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6629 10
Size: 54 1425 343 4797 3030
Fragments arranged by size:
4797 3030 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3476 3731 3849 7607 7966 8125
Size: 648 1404 906 255 118 3758 359 159
Cuts at: 8125 518
Size: 2042
Fragments arranged by size:
3758 2042 1404 906 648 359 255 159
118
MspA1I CmG'CkG
Cuts at: 1606 2040 2411 4046 7065 7742 8890 9135 1606
Size: 434 371 1635 3019 677 1148 245 2120
Fragments arranged by size:
3019 2120 1635 1148 677 434 371 245
NcoI C'CATG_G
Cuts at: 513 1065 6384 6889 513
Size: 552 5319 505 3273
Fragments arranged by size:
5319 3273 552 505
NdeI CA'TA_TG
Cuts at: 387 3592 4612 4930 387
Size: 3205 1020 318 5106
Fragments arranged by size:
5106 3205 1020 318
NheI G'CTAG_C
Cuts at: 1052 3257 3283 1052
Size: 2205 26 7418
Fragments arranged by size:
7418 2205 26
NotI GC'GGCC_GC
Cuts at: 7096 7096
Size: 9649
NspI r_CATG'y
Cuts at: 1132 1229 2239 5940 7007 8552 1132
Size: 97 1010 3701 1067 1545 2229
Fragments arranged by size:
3701 2229 1545 1067 1010 97
PasI CC'CwG_GG
Cuts at: 1348 6783 1348
Size: 5435 4214
PciI A'CATG_T
Cuts at: 8548 8548
Size: 9649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9649
PfoI T'CCnGG_A
Cuts at: 5719 6231 5719
Size: 512 9137
PmeI GTTT'AAAC
Cuts at: 6953 6953
Size: 9649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7718 7750 9231 9263 7718
Size: 32 1481 32 8104
Fragments arranged by size:
8104 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3269 1613
Size: 1656 7993
PsiI TTA'TAA
Cuts at: 3201 5829 6795 7219 3201
Size: 2628 966 424 5631
Fragments arranged by size:
5631 2628 966 424
PstI C_TGCA'G
Cuts at: 830 2176 2395 3046 7001 830
Size: 1346 219 651 3955 3478
Fragments arranged by size:
3955 3478 1346 651 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9649
PvuII CAG'CTG
Cuts at: 2411 2411
Size: 9649
SacI G_AGCT'C
Cuts at: 5264 6888 6914 5264
Size: 1624 26 7999
Fragments arranged by size:
7999 1624 26
SalI G'TCGA_C
Cuts at: 1936 6991 1936
Size: 5055 4594
SbfI CC_TGCA'GG
Cuts at: 7001 7001
Size: 9649
ScaI AGT'ACT
Cuts at: 2020 2645 7897 9609 2020
Size: 625 5252 1712 2060
Fragments arranged by size:
5252 2060 1712 625
SfcI C'TryA_G
Cuts at: 826 1045 2172 2256 2391 3024 3042 4479 5207
Size: 219 1127 84 135 633 18 1437 728
Cuts at: 5207 5790 6997 8132 8813 9004 826
Size: 583 1207 1135 681 191 1471
Fragments arranged by size:
1471 1437 1207 1135 1127 728 681 633
583 219 191 135 84 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9649
SmaI CCC'GGG
Cuts at: 1872 6976 1872
Size: 5104 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2639 2647 2891 3432 4255
Size: 197 926 168 528 8 244 541 823
Cuts at: 4255 4504 6014 7753 8654 8916 820
Size: 249 1510 1739 901 262 1553
Fragments arranged by size:
1739 1553 1510 926 901 823 541 528
262 249 244 197 168 8
SnaBI TAC'GTA
Cuts at: 493 7440 493
Size: 6947 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9649
SphI G_CATG'C
Cuts at: 1132 7007 1132
Size: 5875 3774
SspI AAT'ATT
Cuts at: 5 52 4210 7573 5
Size: 47 4158 3363 2081
Fragments arranged by size:
4158 3363 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9649
StyI C'CwwG_G
Cuts at: 513 1065 5850 6384 6889 7088 513
Size: 552 4785 534 505 199 3074
Fragments arranged by size:
4785 3074 552 534 505 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2677 3992 6934 6943 7676 7693
Size: 1255 531 566 1315 2942 9 733 17
Cuts at: 7693 7846 8031 8476 325
Size: 153 185 445 1498
Fragments arranged by size:
2942 1498 1315 1255 733 566 531 445
185 153 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2018 2643 2846
Size: 274 80 33 51 1443 41 625 203
Cuts at: 2846 4553 5197 6136 6344 6451 7895 9607 96
Size: 1707 644 939 208 107 1444 1712 138
Fragments arranged by size:
1712 1707 1444 1443 939 644 625 274
208 203 138 107 80 51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9609 9641 9609
Size: 32 9617
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9649
XbaI T'CTAG_A
Cuts at: 5561 6199 6985 5561
Size: 638 786 8225
Fragments arranged by size:
8225 786 638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9649
XmaI C'CCGG_G
Cuts at: 1870 6974 1870
Size: 5104 4545
XmnI GAAnn'nnTTC
Cuts at: 3507 3972 5599 6615 7778 3507
Size: 465 1627 1016 1163 5378
Fragments arranged by size:
5378 1627 1163 1016 465
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7457 276
Size: 53 83 186 936 5923 2468
Fragments arranged by size:
5923 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI