Restriction map for
pFN21AA0041
(Circular) (Six-base) MAPSORT of: pFN21AA0041.seq Check: 7614 from: 1 to: 7039
pFN21AA0041 7039 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4849 278
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4382 1937
Size: 2445 4594
Acc65I G'GTAC_C
Cuts at: 4360 4360
Size: 7039
AclI AA'CG_TT
Cuts at: 1563 5166 5539 1563
Size: 3603 373 3063
Fragments arranged by size:
3603 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2057 2315 2513 3194 3627 3789 3812
Size: 406 19 258 198 681 433 162 23
Cuts at: 3812 5102 6486 6754 1632
Size: 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 681 433 406 268 258
198 162 23 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6842
AflIII A'CryG_T
Cuts at: 1203 3971 5938 6915 1203
Size: 2768 1967 977 1327
Fragments arranged by size:
2768 1967 1327 977
AgeI A'CCGG_T
Cuts at: 5870 5870
Size: 7039
AhdI GACnn_n'nnGTC
Cuts at: 5768 5768
Size: 7039
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7039
AlwNI CAG_nnn'CTG
Cuts at: 2865 4782 6354 2865
Size: 1917 1572 3550
Fragments arranged by size:
3550 1917 1572
ApaLI G'TGCA_C
Cuts at: 2667 5096 6252 2667
Size: 2429 1156 3454
Fragments arranged by size:
3454 2429 1156
ApoI r'AATT_y
Cuts at: 1493 2278 2360 2906 3321 3536 3632 3696 4334
Size: 785 82 546 415 215 96 64 638
Cuts at: 4334 4348 4574 4726 6835 1493
Size: 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 785 638 546 415 226 215
152 96 82 64 14
AseI AT'TA_AT
Cuts at: 160 1032 2535 5593 160
Size: 872 1503 3058 1606
Fragments arranged by size:
3058 1606 1503 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
AvaI C'yCGr_G
Cuts at: 1870 1943 4364 1870
Size: 73 2421 4545
Fragments arranged by size:
4545 2421 73
BamHI G'GATC_C
Cuts at: 4369 4736 4369
Size: 367 6672
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4094 4360 5815 618
Size: 325 217 627 2307 266 1455 1842
Fragments arranged by size:
2307 1842 1455 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 3710 3962 4358 1391
Size: 2319 252 396 4072
Fragments arranged by size:
4072 2319 396 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 2943 2976 928
Size: 971 356 688 33 4991
Fragments arranged by size:
4991 971 688 356 33
BbvCI CC'TCA_GC
Cuts at: 3873 3873
Size: 7039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2970 3004 5230 5264 1903
Size: 34 1033 34 2226 34 3678
Fragments arranged by size:
3678 2226 1033 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4931 6141 3236
Size: 1695 1210 4134
Fragments arranged by size:
4134 1695 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7039
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2603 5650 136
Size: 107 122 71 631 605 931 3047 1525
Fragments arranged by size:
3047 1525 931 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2350 7034 2350
Size: 4684 2355
BlpI GC'TnA_GC
Cuts at: 3620 4456 3620
Size: 836 6203
Bme1580I G_kGCm'C
Cuts at: 1790 2671 4062 5100 6256 1790
Size: 881 1391 1038 1156 2573
Fragments arranged by size:
2573 1391 1156 1038 881
BmgBI CAC'GTC
Cuts at: 1318 3898 3972 1318
Size: 2580 74 4385
Fragments arranged by size:
4385 2580 74
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3913 4770 5728
Size: 416 53 83 399 24 2496 857 958
Cuts at: 5728 442
Size: 1753
Fragments arranged by size:
2496 1753 958 857 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 7039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2740 3011 5699 1239
Size: 1501 271 2688 2579
Fragments arranged by size:
2688 2579 1501 271
Bpu10I CC'TnA_GC
Cuts at: 3573 3873 3573
Size: 300 6739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5164 6029 6327 5164
Size: 865 298 5876
Fragments arranged by size:
5876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2190 3007 5702 882
Size: 617 691 817 2695 2219
Fragments arranged by size:
2695 2219 817 691 617
BsaAI yAC'GTr
Cuts at: 493 1204 4830 493
Size: 711 3626 2702
Fragments arranged by size:
3626 2702 711
BsaBI GATnn'nnATC
Cuts at: 7033 7033
Size: 7039
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3842 4846 5228 5863
Size: 53 83 186 936 2309 1004 382 635
Cuts at: 5863 275
Size: 1451
Fragments arranged by size:
2309 1451 1004 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3317 3327 5472 5870 6144 6291 6702 3317
Size: 10 2145 398 274 147 411 3654
Fragments arranged by size:
3654 2145 411 398 274 147 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6814
Fragments arranged by size:
6814 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 1186
Size: 856 6183
BseYI C'CCAG_C
Cuts at: 6242 6738 6242
Size: 496 6543
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3912 1163
Size: 573 2176 4290
Fragments arranged by size:
4290 2176 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4489 5250 6278 1540
Size: 275 186 2488 761 1028 2301
Fragments arranged by size:
2488 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2671 3317 3710 5100 5185 6256 2671
Size: 646 393 1390 85 1071 3454
Fragments arranged by size:
3454 1390 1071 646 393 85
BsmI GAATG_Cn'
Cuts at: 4550 4643 4550
Size: 93 6946
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 3317 3710 3962 4062 4358 5100
Size: 399 881 646 393 252 100 296 742
Cuts at: 5100 5185 6256 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 646 399 393 296
252 100 85
BspEI T'CCGG_A
Cuts at: 3317 6702 3317
Size: 3385 3654
BspHI T'CATG_A
Cuts at: 2181 4928 6658 2181
Size: 2747 1730 2562
Fragments arranged by size:
2747 2562 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4060 4394 844
Size: 3216 334 3489
Fragments arranged by size:
3489 3216 334
BsrBI CCG'CTC
Cuts at: 1394 1823 4926 1394
Size: 429 3103 3507
Fragments arranged by size:
3507 3103 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 2845 3241 4034 5534 5708 65
Size: 1183 894 703 396 793 1500 174 1396
Fragments arranged by size:
1500 1396 1183 894 793 703 396 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5683 5870 1700
Size: 110 3873 187 2869
Fragments arranged by size:
3873 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7039
BssSI C'ACGA_G
Cuts at: 5099 6111 5099
Size: 1012 6027
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4160 794
Size: 3366 3673
BstBI TT'CG_AA
Cuts at: 2385 5843 2385
Size: 3458 3581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7039
BstYI r'GATC_y
Cuts at: 1972 2350 2659 3265 4369 4736 5121 5138 6590
Size: 378 309 606 1104 367 385 17 1452
Cuts at: 6590 6676 6688 7034 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1104 606 385 378 367 346
309 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2942 2942
Size: 7039
BtgI C'CryG_G
Cuts at: 513 1065 3269 3738 513
Size: 552 2204 469 3814
Fragments arranged by size:
3814 2204 552 469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6300
Fragments arranged by size:
6300 683 56
BtsI GCAGTG_nn'
Cuts at: 4555 5349 5369 4555
Size: 794 20 6225
Fragments arranged by size:
6225 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7039
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3507 3542 529
Size: 35 2943 35 4026
Fragments arranged by size:
4026 2943 35 35
DraI TTT'AAA
Cuts at: 3396 4343 3396
Size: 947 6092
DraIII CAC_nnn'GTG
Cuts at: 3303 3303
Size: 7039
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6046 809
Size: 507 4730 1802
Fragments arranged by size:
4730 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4486 5375 7004
Size: 54 1425 50 275 18 2656 889 1629
Cuts at: 7004 8
Size: 43
Fragments arranged by size:
2656 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4486 1537
Size: 275 2674 4090
Fragments arranged by size:
4090 2674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 4197 4969 1353
Size: 2362 482 772 3423
Fragments arranged by size:
3423 2362 772 482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5626 6000 6146 387
Size: 1064 138 4037 374 146 1280
Fragments arranged by size:
4037 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3708 3708
Size: 7039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2057 2315 2513 2740 3011 3194
Size: 393 406 19 258 198 227 271 183
Cuts at: 3194 3627 3789 3812 5102 5699 6486 6754 1239
Size: 433 162 23 1290 597 787 268 1524
Fragments arranged by size:
1524 1290 787 597 433 406 393 271
268 258 227 198 183 162 23 19
EcoRI G'AATT_C
Cuts at: 3321 3696 4348 4726 3321
Size: 375 652 378 5634
Fragments arranged by size:
5634 652 378 375
EcoRV GAT'ATC
Cuts at: 2819 2819
Size: 7039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2369 3744 2369
Size: 1375 5664
FspI TGC'GCA
Cuts at: 5545 5545
Size: 7039
HaeII r_GCGC'y
Cuts at: 1678 2866 6186 1678
Size: 1188 3320 2531
Fragments arranged by size:
3320 2531 1188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 3119 3151 5680
Size: 32 135 32 978 32 537 32 2529
Cuts at: 5680 5712 5754 5786 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2529 978 537 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4006 4383 4629 669
Size: 988 281 2068 377 246 3079
Fragments arranged by size:
3079 2068 988 377 281 246
HindIII A'AGCT_T
Cuts at: 748 2114 3398 748
Size: 1366 1284 4389
Fragments arranged by size:
4389 1366 1284
HpaI GTT'AAC
Cuts at: 4629 4629
Size: 7039
KpnI G_GTAC'C
Cuts at: 4364 4364
Size: 7039
MfeI C'AATT_G
Cuts at: 4638 4638
Size: 7039
MluI A'CGCG_T
Cuts at: 6915 6915
Size: 7039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2527 2655 2945 6153 6337 1305
Size: 1222 128 290 3208 184 2007
Fragments arranged by size:
3208 2007 1222 290 184 128
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5217
Fragments arranged by size:
5217 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2807 4145 4997 5356 5515 518
Size: 648 843 798 1338 852 359 159 2042
Fragments arranged by size:
2042 1338 852 843 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3214 3243 4455 5132 6280 6525 1606
Size: 1608 29 1212 677 1148 245 2120
Fragments arranged by size:
2120 1608 1212 1148 677 245 29
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6487
NdeI CA'TA_TG
Cuts at: 387 3847 387
Size: 3460 3579
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7039
NotI GC'GGCC_GC
Cuts at: 4486 4486
Size: 7039
NspI r_CATG'y
Cuts at: 1132 1229 3025 4109 4397 5942 1132
Size: 97 1796 1084 288 1545 2229
Fragments arranged by size:
2229 1796 1545 1084 288 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7039
PciI A'CATG_T
Cuts at: 5938 5938
Size: 7039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7039
PfoI T'CCnGG_A
Cuts at: 3820 3820
Size: 7039
PmeI GTTT'AAAC
Cuts at: 4343 4343
Size: 7039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5108 5140 6621 6653 5108
Size: 32 1481 32 5494
Fragments arranged by size:
5494 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7039
PsiI TTA'TAA
Cuts at: 3428 4609 3428
Size: 1181 5858
PstI C_TGCA'G
Cuts at: 830 4391 830
Size: 3561 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7039
PvuII CAG'CTG
Cuts at: 3243 3243
Size: 7039
SacI G_AGCT'C
Cuts at: 3710 3710
Size: 7039
SalI G'TCGA_C
Cuts at: 1936 4381 1936
Size: 2445 4594
SbfI CC_TGCA'GG
Cuts at: 4391 4391
Size: 7039
ScaI AGT'ACT
Cuts at: 5287 6999 5287
Size: 1712 5327
SfcI C'TryA_G
Cuts at: 826 1045 3986 4387 5522 6203 6394 826
Size: 219 2941 401 1135 681 191 1471
Fragments arranged by size:
2941 1471 1135 681 401 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7039
SmaI CCC'GGG
Cuts at: 1872 4366 1872
Size: 2494 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5143 6044 6306 820
Size: 197 926 3200 901 262 1553
Fragments arranged by size:
3200 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4830 493
Size: 4337 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7039
SphI G_CATG'C
Cuts at: 1132 3025 4397 1132
Size: 1893 1372 3774
Fragments arranged by size:
3774 1893 1372
SspI AAT'ATT
Cuts at: 5 52 3551 4963 5
Size: 47 3499 1412 2081
Fragments arranged by size:
3499 2081 1412 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7039
StyI C'CwwG_G
Cuts at: 513 1065 3334 4021 4478 513
Size: 552 2269 687 457 3074
Fragments arranged by size:
3074 2269 687 552 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5066 5083 5236 5421 5866 325
Size: 1255 3486 17 153 185 445 1498
Fragments arranged by size:
3486 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2434 5285 6997
Size: 274 80 33 51 1443 457 2851 1712
Cuts at: 6997 96
Size: 138
Fragments arranged by size:
2851 1712 1443 457 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6999 7031 6999
Size: 32 7007
Tth111I GACn'n_nGTC
Cuts at: 1316 3650 1316
Size: 2334 4705
XbaI T'CTAG_A
Cuts at: 4375 4375
Size: 7039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3227 3286 3227
Size: 59 6980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7039
XmaI C'CCGG_G
Cuts at: 1870 4364 1870
Size: 2494 4545
XmnI GAAnn'nnTTC
Cuts at: 2381 5168 2381
Size: 2787 4252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4847 276
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI