Restriction map for
pFN21AA0029
(Circular) (Six-base) MAPSORT of: pFN21AA0029.seq Check: 3980 from: 1 to: 7618
pFN21AA0029 7618 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5428 278
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4236 4961 1937
Size: 2299 725 4594
Fragments arranged by size:
4594 2299 725
Acc65I G'GTAC_C
Cuts at: 4939 4939
Size: 7618
AclI AA'CG_TT
Cuts at: 1563 2629 5745 6118 1563
Size: 1066 3116 373 3063
Fragments arranged by size:
3116 3063 1066 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2865 3001 3329 3789 3963 4847 5681 7065
Size: 1233 136 328 460 174 884 834 1384
Cuts at: 7065 7333 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1233 884 834 460 328 268
174 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7421
AflIII A'CryG_T
Cuts at: 1203 6517 7494 1203
Size: 5314 977 1327
Fragments arranged by size:
5314 1327 977
AgeI A'CCGG_T
Cuts at: 6449 6449
Size: 7618
AhdI GACnn_n'nnGTC
Cuts at: 4382 4684 6347 4382
Size: 302 1663 5653
Fragments arranged by size:
5653 1663 302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7618
AlwNI CAG_nnn'CTG
Cuts at: 4429 5361 6933 4429
Size: 932 1572 5114
Fragments arranged by size:
5114 1572 932
ApaLI G'TGCA_C
Cuts at: 5675 6831 5675
Size: 1156 6462
ApoI r'AATT_y
Cuts at: 1493 2181 2336 2403 2444 2582 3849 4223 4927
Size: 688 155 67 41 138 1267 374 704
Cuts at: 4927 5153 5305 7414 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1267 704 688 374 226 155
152 138 67 41
AseI AT'TA_AT
Cuts at: 160 1032 6172 160
Size: 872 5140 1606
Fragments arranged by size:
5140 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
AvaI C'yCGr_G
Cuts at: 1870 1943 4388 4943 1870
Size: 73 2445 555 4545
Fragments arranged by size:
4545 2445 555 73
AvrII C'CTAG_G
Cuts at: 3250 3250
Size: 7618
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3578 3611 3578
Size: 33 7585
BamHI G'GATC_C
Cuts at: 4948 5315 4948
Size: 367 7251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2850 4655 4939 6394 618
Size: 325 217 627 1063 1805 284 1455 1842
Fragments arranged by size:
1842 1805 1455 1063 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 4937 1391
Size: 3546 4072
BbeI G_GCGC'C
Cuts at: 2854 4659 2854
Size: 1805 5813
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2622 928
Size: 971 723 5924
Fragments arranged by size:
5924 971 723
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5809 5843 1903
Size: 34 3872 34 3678
Fragments arranged by size:
3872 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4419 5510 6720 4419
Size: 1091 1210 5317
Fragments arranged by size:
5317 1210 1091
BclI T'GATC_A
Cuts at: 1548 2521 2575 4195 1548
Size: 973 54 1620 4971
Fragments arranged by size:
4971 1620 973 54
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6229 136
Size: 107 122 71 631 605 4557 1525
Fragments arranged by size:
4557 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2326 2888 7613 2326
Size: 562 4725 2331
Fragments arranged by size:
4725 2331 562
BlpI GC'TnA_GC
Cuts at: 4308 5035 4308
Size: 727 6891
Bme1580I G_kGCm'C
Cuts at: 1790 4156 5679 6835 1790
Size: 2366 1523 1156 2573
Fragments arranged by size:
2573 2366 1523 1156
BmgBI CAC'GTC
Cuts at: 1318 4697 1318
Size: 3379 4239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3596 4678 4752
Size: 416 53 83 399 24 2179 1082 74
Cuts at: 4752 5349 6307 442
Size: 597 958 1753
Fragments arranged by size:
2179 1753 1082 958 597 416 399 83
74 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7618
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 3297 2242
Size: 1055 6563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2971 3491 3549 3565 6278 1239
Size: 1732 520 58 16 2713 2579
Fragments arranged by size:
2713 2579 1732 520 58 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 3819 3828 5743 6608 6906 2624
Size: 1195 9 1915 865 298 3336
Fragments arranged by size:
3336 1915 1195 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2748 3261 6281 882
Size: 617 1249 513 3020 2219
Fragments arranged by size:
3020 2219 1249 617 513
BsaAI yAC'GTr
Cuts at: 493 1204 2438 5409 493
Size: 711 1234 2971 2702
Fragments arranged by size:
2971 2702 1234 711
BsaBI GATnn'nnATC
Cuts at: 7612 7612
Size: 7618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2851 4656 5425 5807
Size: 53 83 186 936 1318 1805 769 382
Cuts at: 5807 6442 275
Size: 635 1451
Fragments arranged by size:
1805 1451 1318 936 769 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4665 6051 6449 6723 6870 7281 4665
Size: 1386 398 274 147 411 5002
Fragments arranged by size:
5002 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3563 3593 4548 4578 4754
Size: 30 165 30 2160 30 955 30 176
Cuts at: 4754 4784 1178
Size: 30 4012
Fragments arranged by size:
4012 2160 955 176 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3390 3414 3417 3420 1186
Size: 2204 24 3 3 5384
Fragments arranged by size:
5384 2204 24 3 3
BseYI C'CCAG_C
Cuts at: 2841 4050 6821 7317 2841
Size: 1209 2771 496 3142
Fragments arranged by size:
3142 2771 1209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4439 4797 1163
Size: 573 2703 358 3984
Fragments arranged by size:
3984 2703 573 358
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5068 5829 6857 1540
Size: 275 186 3067 761 1028 2301
Fragments arranged by size:
3067 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3507 5679 5764 6835 3507
Size: 2172 85 1071 4290
Fragments arranged by size:
4290 2172 1071 85
BsmI GAATG_Cn'
Cuts at: 3930 4825 5129 5222 3930
Size: 895 304 93 6326
Fragments arranged by size:
6326 895 304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3507 4156 4937 5679 5764 6835 1391
Size: 399 1717 649 781 742 85 1071 2174
Fragments arranged by size:
2174 1717 1071 781 742 649 399 85
BspEI T'CCGG_A
Cuts at: 7281 7281
Size: 7618
BspHI T'CATG_A
Cuts at: 3372 5507 7237 3372
Size: 2135 1730 3753
Fragments arranged by size:
3753 2135 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3001 3661 3999 4973 844
Size: 2157 660 338 974 3489
Fragments arranged by size:
3489 2157 974 660 338
BsrBI CCG'CTC
Cuts at: 1394 1823 5505 1394
Size: 429 3682 3507
Fragments arranged by size:
3682 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6113 6287 65
Size: 1183 4865 174 1396
Fragments arranged by size:
4865 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6262 6449 1700
Size: 110 4452 187 2869
Fragments arranged by size:
4452 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4073 96
Size: 3977 3641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7618
BssSI C'ACGA_G
Cuts at: 5678 6690 5678
Size: 1012 6606
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4820 794
Size: 4026 3592
BstBI TT'CG_AA
Cuts at: 2980 6422 2980
Size: 3442 4176
BstEII G'GTnAC_C
Cuts at: 1184 3986 1184
Size: 2802 4816
BstXI CCAn_nnnn'nTGG
Cuts at: 2949 3173 4765 2949
Size: 224 1592 5802
Fragments arranged by size:
5802 1592 224
BstYI r'GATC_y
Cuts at: 1972 2187 2326 2888 3161 3905 4662 4948 5315
Size: 215 139 562 273 744 757 286 367
Cuts at: 5315 5700 5717 7169 7255 7267 7613 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 757 744 562 385 367 346
286 273 215 139 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7066
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6879
Fragments arranged by size:
6879 683 56
BtsI GCAGTG_nn'
Cuts at: 3641 3827 4175 4237 4427 4726 4731 5134 5928
Size: 186 348 62 190 299 5 403 794
Cuts at: 5928 5948 3641
Size: 20 5311
Fragments arranged by size:
5311 794 403 348 299 190 186 62
20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4528 4563 529
Size: 35 3964 35 3584
Fragments arranged by size:
3964 3584 35 35
DraI TTT'AAA
Cuts at: 4648 4922 4648
Size: 274 7344
DraIII CAC_nnn'GTG
Cuts at: 4100 4100
Size: 7618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4244 6625 809
Size: 507 2928 2381 1802
Fragments arranged by size:
2928 2381 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3751 4420 5065
Size: 54 1425 50 275 18 1921 669 645
Cuts at: 5065 5954 7583 8
Size: 889 1629 43
Fragments arranged by size:
1921 1629 1425 889 669 645 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5065 1537
Size: 275 3253 4090
Fragments arranged by size:
4090 3253 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 2730 2982 3982 5548 1353
Size: 858 519 252 1000 1566 3423
Fragments arranged by size:
3423 1566 1000 858 519 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6205 6579 6725 387
Size: 1064 138 4616 374 146 1280
Fragments arranged by size:
4616 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2865 2971 3001 3329 3491 3549 3565
Size: 393 1233 106 30 328 162 58 16
Cuts at: 3565 3789 3963 4847 5681 6278 7065 7333 1239
Size: 224 174 884 834 597 787 268 1524
Fragments arranged by size:
1524 1233 884 834 787 597 393 328
268 224 174 162 106 58 30 16
EcoO109I rG'GnC_Cy
Cuts at: 4678 4678
Size: 7618
EcoRI G'AATT_C
Cuts at: 3849 4927 5305 3849
Size: 1078 378 6162
Fragments arranged by size:
6162 1078 378
EcoRV GAT'ATC
Cuts at: 3470 3470
Size: 7618
FspI TGC'GCA
Cuts at: 3657 6124 3657
Size: 2467 5151
HaeII r_GCGC'y
Cuts at: 1678 2854 4659 6765 1678
Size: 1176 1805 2106 2531
Fragments arranged by size:
2531 2106 1805 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3655 3687 3777 3809 4368
Size: 32 135 32 2083 32 90 32 559
Cuts at: 4368 4400 4670 4702 4754 4786 6259 6291 6333
Size: 32 270 32 52 32 1473 32 42
Cuts at: 6333 6365 1373
Size: 32 2626
Fragments arranged by size:
2626 2083 1473 559 270 135 90 52
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2196 3959 4849 4962 5208 669
Size: 988 281 258 1763 890 113 246 3079
Fragments arranged by size:
3079 1763 988 890 281 258 246 113
HindIII A'AGCT_T
Cuts at: 748 4628 748
Size: 3880 3738
HpaI GTT'AAC
Cuts at: 2196 4849 5208 2196
Size: 2653 359 4606
Fragments arranged by size:
4606 2653 359
KasI G'GCGC_C
Cuts at: 2850 4655 2850
Size: 1805 5813
KpnI G_GTAC'C
Cuts at: 4943 4943
Size: 7618
MfeI C'AATT_G
Cuts at: 5217 5217
Size: 7618
MluI A'CGCG_T
Cuts at: 7494 7494
Size: 7618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3694 6732 6916 1305
Size: 2389 3038 184 2007
Fragments arranged by size:
3038 2389 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3753 4422 10
Size: 54 1425 343 1921 669 3206
Fragments arranged by size:
3206 1921 1425 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 5576 5935 6094 518
Size: 648 843 3567 359 159 2042
Fragments arranged by size:
3567 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4079 4312 4811 5034 5711 6859 7104 1606
Size: 2473 233 499 223 677 1148 245 2120
Fragments arranged by size:
2473 2120 1148 677 499 245 233 223
NarI GG'CG_CC
Cuts at: 2851 4656 2851
Size: 1805 5813
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7066
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7618
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7618
NotI GC'GGCC_GC
Cuts at: 5065 5065
Size: 7618
NspI r_CATG'y
Cuts at: 1132 1229 4976 6521 1132
Size: 97 3747 1545 2229
Fragments arranged by size:
3747 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2367 1348
Size: 1019 6599
PciI A'CATG_T
Cuts at: 6517 6517
Size: 7618
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4100 4418 1108
Size: 2992 318 4308
Fragments arranged by size:
4308 2992 318
PfoI T'CCnGG_A
Cuts at: 4000 4674 4000
Size: 674 6944
PmeI GTTT'AAAC
Cuts at: 4922 4922
Size: 7618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5687 5719 7200 7232 5687
Size: 32 1481 32 6073
Fragments arranged by size:
6073 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4678 4678
Size: 7618
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7618
PsiI TTA'TAA
Cuts at: 5188 5188
Size: 7618
PstI C_TGCA'G
Cuts at: 830 4434 4970 830
Size: 3604 536 3478
Fragments arranged by size:
3604 3478 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7618
PvuII CAG'CTG
Cuts at: 4079 4312 4811 4079
Size: 233 499 6886
Fragments arranged by size:
6886 499 233
SalI G'TCGA_C
Cuts at: 1936 4960 1936
Size: 3024 4594
SanDI GG'GwC_CC
Cuts at: 4678 4678
Size: 7618
SapI GCTCTTCn'nnn_
Cuts at: 3982 3982
Size: 7618
SbfI CC_TGCA'GG
Cuts at: 4970 4970
Size: 7618
ScaI AGT'ACT
Cuts at: 2933 5866 7578 2933
Size: 2933 1712 2973
Fragments arranged by size:
2973 2933 1712
SexAI A'CCwGG_T
Cuts at: 2676 3952 4096 2676
Size: 1276 144 6198
Fragments arranged by size:
6198 1276 144
SfcI C'TryA_G
Cuts at: 826 1045 2718 3937 4430 4966 6101 6782 6973
Size: 219 1673 1219 493 536 1135 681 191
Cuts at: 6973 826
Size: 1471
Fragments arranged by size:
1673 1471 1219 1135 681 536 493 219
191
SfoI GGC'GCC
Cuts at: 2852 4657 2852
Size: 1805 5813
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7618
SmaI CCC'GGG
Cuts at: 1872 4390 4945 1872
Size: 2518 555 4545
Fragments arranged by size:
4545 2518 555
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3834 3843 5722 6623 6885
Size: 197 926 696 1195 9 1879 901 262
Cuts at: 6885 820
Size: 1553
Fragments arranged by size:
1879 1553 1195 926 901 696 262 197
9
SnaBI TAC'GTA
Cuts at: 493 5409 493
Size: 4916 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7618
SphI G_CATG'C
Cuts at: 1132 4976 1132
Size: 3844 3774
SspI AAT'ATT
Cuts at: 5 52 2763 4187 4243 5542 5
Size: 47 2711 1424 56 1299 2081
Fragments arranged by size:
2711 2081 1424 1299 56 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7618
StyI C'CwwG_G
Cuts at: 513 1065 3250 3703 4546 5057 513
Size: 552 2185 453 843 511 3074
Fragments arranged by size:
3074 2185 843 552 511 453
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2772 5645 5662 5815 6000 6445 325
Size: 1255 1192 2873 17 153 185 445 1498
Fragments arranged by size:
2873 1498 1255 1192 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 4015 4073
Size: 274 80 33 51 1443 954 1084 58
Cuts at: 4073 4434 5864 7576 96
Size: 361 1430 1712 138
Fragments arranged by size:
1712 1443 1430 1084 954 361 274 138
80 58 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 7578 7610 3563
Size: 32 3983 32 3571
Fragments arranged by size:
3983 3571 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7618
XbaI T'CTAG_A
Cuts at: 4373 4954 4373
Size: 581 7037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3710 4076 3710
Size: 366 7252
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7618
XmaI C'CCGG_G
Cuts at: 1870 4388 4943 1870
Size: 2518 555 4545
Fragments arranged by size:
4545 2518 555
XmnI GAAnn'nnTTC
Cuts at: 2619 2764 4033 5747 2619
Size: 145 1269 1714 4490
Fragments arranged by size:
4490 1714 1269 145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5426 276
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI