Restriction map for
pFN21AA0015
(Circular) (Six-base) MAPSORT of: pFN21AA0015.seq Check: 4121 from: 1 to: 6067
pFN21AA0015 6067 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2422
Size: 6067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3099 3877 278
Size: 53 83 186 936 1563 778 2468
Fragments arranged by size:
2468 1563 936 778 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4130 5514 5782 1632
Size: 2498 1384 268 1917
Fragments arranged by size:
2498 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 2523 3012 4966 5943 1203
Size: 1320 489 1954 977 1327
Fragments arranged by size:
1954 1327 1320 977 489
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2774 1166
Size: 1608 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2470 2684 3341 3810 5382 2470
Size: 214 657 469 1572 3155
Fragments arranged by size:
3155 1572 657 469 214
ApaLI G'TGCA_C
Cuts at: 2642 4124 5280 2642
Size: 1482 1156 3429
Fragments arranged by size:
3429 1482 1156
ApoI r'AATT_y
Cuts at: 1493 2285 3376 3602 3754 5863 1493
Size: 792 1091 226 152 2109 1697
Fragments arranged by size:
2109 1697 1091 792 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2448 2819 3039 3195 3392 1870
Size: 73 505 371 220 156 197 4545
Fragments arranged by size:
4545 505 371 220 197 156 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2434 2765 3388 4843 618
Size: 325 217 627 647 331 623 1455 1842
Fragments arranged by size:
1842 1455 647 627 623 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2695 3056 3165 3209 3386 1391
Size: 629 675 361 109 44 177 4072
Fragments arranged by size:
4072 675 629 361 177 109 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2989 3229 928
Size: 971 1090 240 3766
Fragments arranged by size:
3766 1090 971 240
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 2150 2181 3484 2150
Size: 31 1303 4733
Fragments arranged by size:
4733 1303 31
Bme1580I G_kGCm'C
Cuts at: 1790 2646 4128 5284 1790
Size: 856 1482 1156 2573
Fragments arranged by size:
2573 1482 1156 856
BmgBI CAC'GTC
Cuts at: 1318 2809 1318
Size: 1491 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2962 2971 3284 3310 4727 1239
Size: 795 928 9 313 26 1417 2579
Fragments arranged by size:
2579 1417 928 795 313 26 9
Bpu10I CC'TnA_GC
Cuts at: 2052 2052
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2479 3338 4730 882
Size: 617 552 428 859 1392 2219
Fragments arranged by size:
2219 1392 859 617 552 428
BsaAI yAC'GTr
Cuts at: 493 1204 2524 2641 3013 3858 493
Size: 711 1320 117 372 845 2702
Fragments arranged by size:
2702 1320 845 711 372 117
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3096 3874 4256 4891
Size: 53 83 186 936 1563 778 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1563 1451 936 778 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2490 4500 4898 5172 5319 5730 2490
Size: 2010 398 274 147 411 2827
Fragments arranged by size:
2827 2010 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3308 3337 3338 3367 1178
Size: 30 165 30 1905 29 1 29 3878
Fragments arranged by size:
3878 1905 165 30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2174 2333 2336 2351 3191 3322 1186
Size: 883 105 159 3 15 840 131 3931
Fragments arranged by size:
3931 883 840 159 131 105 15 3
BseYI C'CCAG_C
Cuts at: 2095 5270 5766 2095
Size: 3175 496 2396
Fragments arranged by size:
3175 2396 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2789 1163
Size: 573 1053 4441
Fragments arranged by size:
4441 1053 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2646 4128 4213 5284 2151
Size: 495 1482 85 1071 2934
Fragments arranged by size:
2934 1482 1071 495 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2980 2980
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2151 2646 2695 3056 3165 3209
Size: 399 230 131 495 49 361 109 44
Cuts at: 3209 3386 4128 4213 5284 1391
Size: 177 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 361 230 177
131 109 85 49 44
BspEI T'CCGG_A
Cuts at: 2490 5730 2490
Size: 3240 2827
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 3075 3422 844
Size: 1578 653 347 3489
Fragments arranged by size:
3489 1578 653 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2752 2763 3202 3954 1394
Size: 429 929 11 439 752 3507
Fragments arranged by size:
3507 929 752 439 429 11
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4562 4736 65
Size: 1183 1539 1775 174 1396
Fragments arranged by size:
1775 1539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2414 2517 4711 4898 1700
Size: 110 604 103 2194 187 2869
Fragments arranged by size:
2869 2194 604 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 2906 1925
Size: 981 5086
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3319 794
Size: 2525 3542
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062
Size: 1425 367 385 17 1452 86 12 346
Cuts at: 6062 1972
Size: 1977
Fragments arranged by size:
1977 1452 1425 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2685 3242 2685
Size: 557 5510
BtgI C'CryG_G
Cuts at: 513 1065 2126 2190 513
Size: 552 1061 64 4390
Fragments arranged by size:
4390 1061 552 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 3583 4377 4397 2471
Size: 1112 794 20 4141
Fragments arranged by size:
4141 1112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2187 2416
Size: 54 1425 50 275 18 210 147 229
Cuts at: 2416 2970 3514 4403 6032 8
Size: 554 544 889 1629 43
Fragments arranged by size:
1629 1425 889 554 544 275 229 210
147 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2310 2555 3997 1353
Size: 668 289 245 1442 3423
Fragments arranged by size:
3423 1442 668 289 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2962 2971 3284 3310 4130 4727
Size: 393 402 928 9 313 26 820 597
Cuts at: 4727 5514 5782 1239
Size: 787 268 1524
Fragments arranged by size:
1524 928 820 787 597 402 393 313
268 26 9
EcoO109I rG'GnC_Cy
Cuts at: 2116 2134 2215 2347 2964 3247 3289 2116
Size: 18 81 132 617 283 42 4894
Fragments arranged by size:
4894 617 283 132 81 42 18
EcoRI G'AATT_C
Cuts at: 2285 3376 3754 2285
Size: 1091 378 4598
Fragments arranged by size:
4598 1091 378
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 3047 5214 1678
Size: 1369 2167 2531
Fragments arranged by size:
2531 2167 1369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3308 3337 3340 3369 4708
Size: 32 135 32 1736 29 3 29 1339
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1736 1339 135 42 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2959 3411 3657 669
Size: 988 281 1021 452 246 3079
Fragments arranged by size:
3079 1021 988 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5181 5365 1305
Size: 3876 184 2007
Fragments arranged by size:
3876 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2189 10
Size: 54 1425 343 210 147 3888
Fragments arranged by size:
3888 1425 343 210 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2774 3227 4025 4384 4543 518
Size: 648 1608 453 798 359 159 2042
Fragments arranged by size:
2042 1608 798 648 453 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2461 2633 3159 3483 4160 5308 5553
Size: 657 198 172 526 324 677 1148 245
Cuts at: 5553 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 657 526 324 245 198
172
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2126 2190 513
Size: 552 1061 64 4390
Fragments arranged by size:
4390 1061 552 64
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2138 2301 1348
Size: 790 163 5114
Fragments arranged by size:
5114 790 163
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 1108
Size: 1269 4798
PfoI T'CCnGG_A
Cuts at: 2070 2070
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PmlI CAC'GTG
Cuts at: 2524 2641 2524
Size: 117 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2964 3247 3289 2116
Size: 848 283 42 4894
Fragments arranged by size:
4894 848 283 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 6035
PstI C_TGCA'G
Cuts at: 830 2760 3419 830
Size: 1930 659 3478
Fragments arranged by size:
3478 1930 659
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2263 2263
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SanDI GG'GwC_CC
Cuts at: 2964 3247 3289 2964
Size: 283 42 5742
Fragments arranged by size:
5742 283 42
SapI GCTCTTCn'nnn_
Cuts at: 2021 2555 2021
Size: 534 5533
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2267 2756 3415 4550 5231 5422 826
Size: 219 1222 489 659 1135 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 659 489 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 2450 3041 3197 3394 1872
Size: 578 591 156 197 4545
Fragments arranged by size:
4545 591 578 197 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4171 5072 5334 820
Size: 197 926 2228 901 262 1553
Fragments arranged by size:
2228 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SrfI GCCC'GGGC
Cuts at: 2450 2450
Size: 6067
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2126 2190 3506 513
Size: 552 1061 64 1316 3074
Fragments arranged by size:
3074 1316 1061 552 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 4094 4111 4264 4449 4894 325
Size: 1255 1332 1182 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 1182 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4313 6025 96
Size: 274 80 33 51 1443 2336 1712 138
Fragments arranged by size:
2336 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 6027 6059 2844
Size: 32 3151 32 2852
Fragments arranged by size:
3151 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 3116 2197
Size: 919 5148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 2448 3039 3195 3392 1870
Size: 578 591 156 197 4545
Fragments arranged by size:
4545 591 578 197 156
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3097 3875 276
Size: 53 83 186 936 1563 778 2468
Fragments arranged by size:
2468 1563 936 778 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI RsrII SacI SacII SexAI SfiI SfoI SwaI