Restriction map for
pFN21AA0012
(Circular) (Six-base) MAPSORT of: pFN21AA0012.seq Check: 8677 from: 1 to: 7063
pFN21AA0012 7063 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4067 4067
Size: 7063
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4873 278
Size: 53 83 186 936 3337 2468
Fragments arranged by size:
3337 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4406 1937
Size: 2469 4594
Acc65I G'GTAC_C
Cuts at: 4384 4384
Size: 7063
AclI AA'CG_TT
Cuts at: 1563 5190 5563 1563
Size: 3627 373 3063
Fragments arranged by size:
3627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2091 2854 5126 6510 6778 1632
Size: 401 58 763 2272 1384 268 1917
Fragments arranged by size:
2272 1917 1384 763 401 268 58
AflII C'TTAA_G
Cuts at: 820 1017 4318 820
Size: 197 3301 3565
Fragments arranged by size:
3565 3301 197
AflIII A'CryG_T
Cuts at: 1203 3717 5962 6939 1203
Size: 2514 2245 977 1327
Fragments arranged by size:
2514 2245 1327 977
AgeI A'CCGG_T
Cuts at: 5894 5894
Size: 7063
AhdI GACnn_n'nnGTC
Cuts at: 5792 5792
Size: 7063
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 1184
Size: 1338 5725
AlwNI CAG_nnn'CTG
Cuts at: 3005 4806 6378 3005
Size: 1801 1572 3690
Fragments arranged by size:
3690 1801 1572
ApaLI G'TGCA_C
Cuts at: 5120 6276 5120
Size: 1156 5907
ApoI r'AATT_y
Cuts at: 1493 2081 2273 2429 2606 2779 2992 3226 3605
Size: 588 192 156 177 173 213 234 379
Cuts at: 3605 4372 4598 4750 6859 1493
Size: 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 588 379 234 226 213
192 177 173 156 152
AseI AT'TA_AT
Cuts at: 160 1032 4332 5617 160
Size: 872 3300 1285 1606
Fragments arranged by size:
3300 1606 1285 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
AvaI C'yCGr_G
Cuts at: 1870 1943 4388 1870
Size: 73 2445 4545
Fragments arranged by size:
4545 2445 73
BamHI G'GATC_C
Cuts at: 2793 4393 4760 2793
Size: 1600 367 5096
Fragments arranged by size:
5096 1600 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3111 4122 4384 5839 618
Size: 325 217 627 1324 1011 262 1455 1842
Fragments arranged by size:
1842 1455 1324 1011 627 325 262 217
BanII G_rGCy'C
Cuts at: 1391 4382 1391
Size: 2991 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2547 3288 928
Size: 971 648 741 4703
Fragments arranged by size:
4703 971 741 648
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5254 5288 1903
Size: 34 3317 34 3678
Fragments arranged by size:
3678 3317 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4955 6165 4955
Size: 1210 5853
BclI T'GATC_A
Cuts at: 1548 3489 3496 1548
Size: 1941 7 5115
Fragments arranged by size:
5115 1941 7
BfrBI ATG'CAT
Cuts at: 2378 3920 2378
Size: 1542 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5674 136
Size: 107 122 71 631 605 4002 1525
Fragments arranged by size:
4002 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7058 7058
Size: 7063
BlpI GC'TnA_GC
Cuts at: 4480 4480
Size: 7063
Bme1580I G_kGCm'C
Cuts at: 1790 3114 3942 5124 6280 1790
Size: 1324 828 1182 1156 2573
Fragments arranged by size:
2573 1324 1182 1156 828
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4794 5752 442
Size: 416 53 83 399 24 3377 958 1753
Fragments arranged by size:
3377 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 3893 5723 1239
Size: 1544 1110 1830 2579
Fragments arranged by size:
2579 1830 1544 1110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 5188 6053 6351 2336
Size: 2852 865 298 3048
Fragments arranged by size:
3048 2852 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 5726 882
Size: 617 615 3612 2219
Fragments arranged by size:
3612 2219 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 4854 493
Size: 711 3650 2702
Fragments arranged by size:
3650 2702 711
BsaBI GATnn'nnATC
Cuts at: 7057 7057
Size: 7063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4870 5252 5887 275
Size: 53 83 186 936 3337 382 635 1451
Fragments arranged by size:
3337 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5496 5894 6168 6315 6726 5496
Size: 398 274 147 411 5833
Fragments arranged by size:
5833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3594 3624 1178
Size: 30 165 30 2191 30 4617
Fragments arranged by size:
4617 2191 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7063
BseYI C'CCAG_C
Cuts at: 6266 6762 6266
Size: 496 6567
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 1163
Size: 573 828 5662
Fragments arranged by size:
5662 828 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4513 5274 6302 1540
Size: 275 186 2512 761 1028 2301
Fragments arranged by size:
2512 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 5124 5209 6280 2447
Size: 2677 85 1071 3230
Fragments arranged by size:
3230 2677 1071 85
BsmI GAATG_Cn'
Cuts at: 3381 4574 4667 3381
Size: 1193 93 5777
Fragments arranged by size:
5777 1193 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 3114 3942 4382 5124 5209 6280
Size: 399 657 667 828 440 742 85 1071
Cuts at: 6280 1391
Size: 2174
Fragments arranged by size:
2174 1071 828 742 667 657 440 399
85
BspEI T'CCGG_A
Cuts at: 6726 6726
Size: 7063
BspHI T'CATG_A
Cuts at: 4009 4162 4952 6682 4009
Size: 153 790 1730 4390
Fragments arranged by size:
4390 1730 790 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4067 4418 844
Size: 3223 351 3489
Fragments arranged by size:
3489 3223 351
BsrBI CCG'CTC
Cuts at: 1394 1823 4950 1394
Size: 429 3127 3507
Fragments arranged by size:
3507 3127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5558 5732 65
Size: 1183 4310 174 1396
Fragments arranged by size:
4310 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5707 5894 1700
Size: 110 3897 187 2869
Fragments arranged by size:
3897 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7063
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7063
BssSI C'ACGA_G
Cuts at: 5123 6135 5123
Size: 1012 6051
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2384 794
Size: 1590 5473
BstBI TT'CG_AA
Cuts at: 2975 5867 2975
Size: 2892 4171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7063
BstXI CCAn_nnnn'nTGG
Cuts at: 3775 3775
Size: 7063
BstYI r'GATC_y
Cuts at: 1972 2793 3817 4169 4393 4760 5145 5162 6614
Size: 821 1024 352 224 367 385 17 1452
Cuts at: 6614 6700 6712 7058 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1024 821 385 367 352 346
224 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2547 2547
Size: 7063
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2946 487
Size: 683 56 1720 4604
Fragments arranged by size:
4604 1720 683 56
BtsI GCAGTG_nn'
Cuts at: 4579 5373 5393 4579
Size: 794 20 6249
Fragments arranged by size:
6249 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7063
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3011 3046 529
Size: 35 2447 35 4546
Fragments arranged by size:
4546 2447 35 35
DraI TTT'AAA
Cuts at: 3288 4367 3288
Size: 1079 5984
DraIII CAC_nnn'GTG
Cuts at: 2587 2587
Size: 7063
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6070 809
Size: 507 4754 1802
Fragments arranged by size:
4754 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4254 4510 5399
Size: 54 1425 50 275 18 2424 256 889
Cuts at: 5399 7028 8
Size: 1629 43
Fragments arranged by size:
2424 1629 1425 889 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4510 1537
Size: 275 2698 4090
Fragments arranged by size:
4090 2698 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4064 4993 1353
Size: 2711 929 3423
Fragments arranged by size:
3423 2711 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5650 6024 6170 387
Size: 1064 138 4061 374 146 1280
Fragments arranged by size:
4061 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2091 2783 2854 3893 5126 5723
Size: 393 401 58 692 71 1039 1233 597
Cuts at: 5723 6510 6778 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1233 1039 787 692 597 401 393
268 71 58
EcoNI CCTnn'n_nnAGG
Cuts at: 3704 3704
Size: 7063
EcoO109I rG'GnC_Cy
Cuts at: 2551 2551
Size: 7063
EcoRI G'AATT_C
Cuts at: 2606 2779 3226 4372 4750 2606
Size: 173 447 1146 378 4919
Fragments arranged by size:
4919 1146 447 378 173
EcoRV GAT'ATC
Cuts at: 2260 2260
Size: 7063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4102 4102
Size: 7063
FspI TGC'GCA
Cuts at: 5569 5569
Size: 7063
HaeII r_GCGC'y
Cuts at: 1678 6210 1678
Size: 4532 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2250 3184 3216
Size: 32 135 32 614 32 32 934 32
Cuts at: 3216 3481 3513 3594 3614 3626 3646 5704 5736
Size: 265 32 81 20 12 20 2058 32
Cuts at: 5736 5778 5810 1373
Size: 42 32 2626
Fragments arranged by size:
2626 2058 934 614 265 135 81 42
32 32 32 32 32 32 32 32
20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4407 4653 669
Size: 988 281 2469 246 3079
Fragments arranged by size:
3079 2469 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3408 748
Size: 2660 4403
HpaI GTT'AAC
Cuts at: 4653 4653
Size: 7063
KpnI G_GTAC'C
Cuts at: 4388 4388
Size: 7063
MfeI C'AATT_G
Cuts at: 2478 3209 4662 2478
Size: 731 1453 4879
Fragments arranged by size:
4879 1453 731
MluI A'CGCG_T
Cuts at: 6939 6939
Size: 7063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 6177 6361 1305
Size: 1800 3072 184 2007
Fragments arranged by size:
3072 2007 1800 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4256 10
Size: 54 1425 343 2424 2817
Fragments arranged by size:
2817 2424 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3583 3776 5021 5380 5539 518
Size: 648 2417 193 1245 359 159 2042
Fragments arranged by size:
2417 2042 1245 648 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 2803 2863 4479 5156 6304 6549 1606
Size: 1197 60 1616 677 1148 245 2120
Fragments arranged by size:
2120 1616 1197 1148 677 245 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6511
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7063
NotI GC'GGCC_GC
Cuts at: 4510 4510
Size: 7063
NsiI A_TGCA'T
Cuts at: 2380 3922 2380
Size: 1542 5521
NspI r_CATG'y
Cuts at: 1132 1229 3028 3721 4421 5966 1132
Size: 97 1799 693 700 1545 2229
Fragments arranged by size:
2229 1799 1545 700 693 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7063
PciI A'CATG_T
Cuts at: 3717 5962 3717
Size: 2245 4818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 1108
Size: 1179 5884
PfoI T'CCnGG_A
Cuts at: 3454 3454
Size: 7063
PmeI GTTT'AAAC
Cuts at: 4367 4367
Size: 7063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5132 5164 6645 6677 5132
Size: 32 1481 32 5518
Fragments arranged by size:
5518 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7063
PsiI TTA'TAA
Cuts at: 3666 4633 3666
Size: 967 6096
PstI C_TGCA'G
Cuts at: 830 3809 4415 830
Size: 2979 606 3478
Fragments arranged by size:
3478 2979 606
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7063
PvuII CAG'CTG
Cuts at: 2803 2863 2803
Size: 60 7003
SalI G'TCGA_C
Cuts at: 1936 4405 1936
Size: 2469 4594
SbfI CC_TGCA'GG
Cuts at: 4415 4415
Size: 7063
ScaI AGT'ACT
Cuts at: 4214 5311 7023 4214
Size: 1097 1712 4254
Fragments arranged by size:
4254 1712 1097
SfcI C'TryA_G
Cuts at: 826 1045 2640 2852 3805 4411 5546 6227 6418
Size: 219 1595 212 953 606 1135 681 191
Cuts at: 6418 826
Size: 1471
Fragments arranged by size:
1595 1471 1135 953 681 606 219 212
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7063
SmaI CCC'GGG
Cuts at: 1872 4390 1872
Size: 2518 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 4318 5167 6068 6330 820
Size: 197 926 408 1967 849 901 262 1553
Fragments arranged by size:
1967 1553 926 901 849 408 262 197
SnaBI TAC'GTA
Cuts at: 493 4854 493
Size: 4361 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7063
SphI G_CATG'C
Cuts at: 1132 4421 1132
Size: 3289 3774
SspI AAT'ATT
Cuts at: 5 52 4336 4987 5
Size: 47 4284 651 2081
Fragments arranged by size:
4284 2081 651 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 5674
StyI C'CwwG_G
Cuts at: 513 1065 4281 4502 513
Size: 552 3216 221 3074
Fragments arranged by size:
3216 3074 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3531 3943 5090 5107 5260 5445 5890
Size: 1255 1951 412 1147 17 153 185 445
Cuts at: 5890 325
Size: 1498
Fragments arranged by size:
1951 1498 1255 1147 445 412 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4212 5309 7021
Size: 274 80 33 51 1443 2235 1097 1712
Cuts at: 7021 96
Size: 138
Fragments arranged by size:
2235 1712 1443 1097 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3003 3035 7023 7055 3003
Size: 32 3988 32 3011
Fragments arranged by size:
3988 3011 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7063
XbaI T'CTAG_A
Cuts at: 4399 4399
Size: 7063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3778 3778
Size: 7063
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7063
XmaI C'CCGG_G
Cuts at: 1870 4388 1870
Size: 2518 4545
XmnI GAAnn'nnTTC
Cuts at: 2783 4203 5192 2783
Size: 1420 989 4654
Fragments arranged by size:
4654 1420 989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4871 276
Size: 53 83 186 936 3337 2468
Fragments arranged by size:
3337 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI