Restriction map for
pFN21AA0010
(Circular) (Six-base) MAPSORT of: pFN21AA0010.seq Check: 1844 from: 1 to: 7954
pFN21AA0010 7954 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5162 5162
Size: 7954
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5764 278
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5297 1937
Size: 3360 4594
Acc65I G'GTAC_C
Cuts at: 3322 3430 5275 3322
Size: 108 1845 6001
Fragments arranged by size:
6001 1845 108
AclI AA'CG_TT
Cuts at: 1563 2341 6081 6454 1563
Size: 778 3740 373 3063
Fragments arranged by size:
3740 3063 778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 3425 4367 4643 5060 5189 6017 7401
Size: 1580 213 942 276 417 129 828 1384
Cuts at: 7401 7669 1632
Size: 268 1917
Fragments arranged by size:
1917 1580 1384 942 828 417 276 268
213 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7757
AflIII A'CryG_T
Cuts at: 1203 3949 6853 7830 1203
Size: 2746 2904 977 1327
Fragments arranged by size:
2904 2746 1327 977
AgeI A'CCGG_T
Cuts at: 6785 6785
Size: 7954
AhdI GACnn_n'nnGTC
Cuts at: 6683 6683
Size: 7954
AleI CACnn'nnGTG
Cuts at: 1166 4670 1166
Size: 3504 4450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 5180 1184
Size: 1299 2697 3958
Fragments arranged by size:
3958 2697 1299
AlwNI CAG_nnn'CTG
Cuts at: 2308 5154 5697 7269 2308
Size: 2846 543 1572 2993
Fragments arranged by size:
2993 2846 1572 543
ApaI G_GGCC'C
Cuts at: 3001 3001
Size: 7954
ApaLI G'TGCA_C
Cuts at: 4265 4902 6011 7167 4265
Size: 637 1109 1156 5052
Fragments arranged by size:
5052 1156 1109 637
ApoI r'AATT_y
Cuts at: 1493 2595 2709 3557 3862 4609 4712 4881 5263
Size: 1102 114 848 305 747 103 169 382
Cuts at: 5263 5489 5641 7750 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1102 848 747 382 305 226
169 152 114 103
AseI AT'TA_AT
Cuts at: 160 1032 4173 4892 6508 160
Size: 872 3141 719 1616 1606
Fragments arranged by size:
3141 1616 1606 872 719
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
AvaI C'yCGr_G
Cuts at: 1870 1943 2691 4850 5177 5279 1870
Size: 73 748 2159 327 102 4545
Fragments arranged by size:
4545 2159 748 327 102 73
BamHI G'GATC_C
Cuts at: 4257 5284 5651 4257
Size: 1027 367 6560
Fragments arranged by size:
6560 1027 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 3430 5275 6730 618
Size: 325 217 627 1535 108 1845 1455 1842
Fragments arranged by size:
1845 1842 1535 1455 627 325 217 108
BanII G_rGCy'C
Cuts at: 1391 2892 3001 5273 1391
Size: 1501 109 2272 4072
Fragments arranged by size:
4072 2272 1501 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 3259 4641 4981 928
Size: 971 438 922 1382 340 3901
Fragments arranged by size:
3901 1382 971 922 438 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3642 3676 6145 6179 1903
Size: 34 1705 34 2469 34 3678
Fragments arranged by size:
3678 2469 1705 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5103 5846 7056 5103
Size: 743 1210 6001
Fragments arranged by size:
6001 1210 743
BclI T'GATC_A
Cuts at: 1548 3414 4870 1548
Size: 1866 1456 4632
Fragments arranged by size:
4632 1866 1456
BfrBI ATG'CAT
Cuts at: 3659 3774 3659
Size: 115 7839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6565 136
Size: 107 122 71 631 605 4893 1525
Fragments arranged by size:
4893 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4104 7949 4104
Size: 3845 4109
BlpI GC'TnA_GC
Cuts at: 5249 5371 5249
Size: 122 7832
Bme1580I G_kGCm'C
Cuts at: 1790 3001 4269 4906 6015 7171 1790
Size: 1211 1268 637 1109 1156 2573
Fragments arranged by size:
2573 1268 1211 1156 1109 637
BmgBI CAC'GTC
Cuts at: 1318 4006 4564 1318
Size: 2688 558 4708
Fragments arranged by size:
4708 2688 558
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5685 6643 442
Size: 416 53 83 399 24 4268 958 1753
Fragments arranged by size:
4268 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2571 1056
Size: 1515 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4212 5014 6614 1239
Size: 2973 802 1600 2579
Fragments arranged by size:
2973 2579 1600 802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3124 5150 6079 6944 7242 3124
Size: 2026 929 865 298 3836
Fragments arranged by size:
3836 2026 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2637 6617 882
Size: 617 1138 3980 2219
Fragments arranged by size:
3980 2219 1138 617
BsaAI yAC'GTr
Cuts at: 493 1204 5745 493
Size: 711 4541 2702
Fragments arranged by size:
4541 2702 711
BsaBI GATnn'nnATC
Cuts at: 7948 7948
Size: 7954
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5761 6143 6778 275
Size: 53 83 186 936 4228 382 635 1451
Fragments arranged by size:
4228 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2761 4749 6387 6785 7059 7206 7617 2761
Size: 1988 1638 398 274 147 411 3098
Fragments arranged by size:
3098 1988 1638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4568 4598 5105 5135 1178
Size: 30 165 30 3165 30 507 30 3997
Fragments arranged by size:
3997 3165 507 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2816 3125 3128 3275 3871 4661 1186
Size: 1630 309 3 147 596 790 4479
Fragments arranged by size:
4479 1630 790 596 309 147 3
BseYI C'CCAG_C
Cuts at: 7157 7653 7157
Size: 496 7458
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3326 1163
Size: 573 1590 5791
Fragments arranged by size:
5791 1590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5404 6165 7193 1540
Size: 275 186 3403 761 1028 2301
Fragments arranged by size:
3403 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2950 4269 4906 6015 6100 7171 2950
Size: 1319 637 1109 85 1071 3733
Fragments arranged by size:
3733 1319 1109 1071 637 85
BsmI GAATG_Cn'
Cuts at: 2520 2905 3191 4479 5465 5558 2520
Size: 385 286 1288 986 93 4916
Fragments arranged by size:
4916 1288 986 385 286 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2892 2950 3001 4269 4906 5273 6015
Size: 399 1102 58 51 1268 637 367 742
Cuts at: 6015 6100 7171 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1268 1102 1071 742 637 399 367
85 58 51
BspEI T'CCGG_A
Cuts at: 2761 7617 2761
Size: 4856 3098
BspHI T'CATG_A
Cuts at: 5843 7573 5843
Size: 1730 6224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2451 4687 5162 5172 5309 844
Size: 1607 2236 475 10 137 3489
Fragments arranged by size:
3489 2236 1607 475 137 10
BsrBI CCG'CTC
Cuts at: 1394 1823 5134 5841 1394
Size: 429 3311 707 3507
Fragments arranged by size:
3507 3311 707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 2735 6449 6623 65
Size: 1183 1017 470 3714 174 1396
Fragments arranged by size:
3714 1396 1183 1017 470 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6598 6785 1700
Size: 110 4788 187 2869
Fragments arranged by size:
4788 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7954
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7954
BssSI C'ACGA_G
Cuts at: 3853 6014 7026 3853
Size: 2161 1012 4781
Fragments arranged by size:
4781 2161 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7954
BstBI TT'CG_AA
Cuts at: 2016 6758 2016
Size: 4742 3212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7954
BstXI CCAn_nnnn'nTGG
Cuts at: 3882 3882
Size: 7954
BstYI r'GATC_y
Cuts at: 1972 2405 4104 4257 4797 5284 5651 6036 6053
Size: 433 1699 153 540 487 367 385 17
Cuts at: 6053 7505 7591 7603 7949 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1699 1452 540 487 433 385 367
346 153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4596 4596
Size: 7954
BtgI C'CryG_G
Cuts at: 513 1065 3276 4282 513
Size: 552 2211 1006 4185
Fragments arranged by size:
4185 2211 1006 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7215
Fragments arranged by size:
7215 683 56
BtsI GCAGTG_nn'
Cuts at: 4807 5470 6264 6284 4807
Size: 663 794 20 6477
Fragments arranged by size:
6477 794 663 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7954
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7919
DraI TTT'AAA
Cuts at: 4335 5258 4335
Size: 923 7031
DraIII CAC_nnn'GTG
Cuts at: 4771 4771
Size: 7954
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4733 6961 809
Size: 507 3417 2228 1802
Fragments arranged by size:
3417 2228 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5401 6290 7919
Size: 54 1425 50 275 18 3571 889 1629
Cuts at: 7919 8
Size: 43
Fragments arranged by size:
3571 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5401 1537
Size: 275 3589 4090
Fragments arranged by size:
4090 3589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3618 4619 5884 1353
Size: 2265 1001 1265 3423
Fragments arranged by size:
3423 2265 1265 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3982 4790 4811 6541 6915 7061
Size: 1064 138 2393 808 21 1730 374 146
Cuts at: 7061 387
Size: 1280
Fragments arranged by size:
2393 1730 1280 1064 808 374 146 138
21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3212 3425 4212 4367 4643 5014 5060
Size: 393 1580 213 787 155 276 371 46
Cuts at: 5060 5189 6017 6614 7401 7669 1239
Size: 129 828 597 787 268 1524
Fragments arranged by size:
1580 1524 828 787 787 597 393 371
276 268 213 155 129 46
EcoNI CCTnn'n_nnAGG
Cuts at: 3005 4688 3005
Size: 1683 6271
EcoO109I rG'GnC_Cy
Cuts at: 2997 2998 4828 4927 2997
Size: 1 1830 99 6024
Fragments arranged by size:
6024 1830 99 1
EcoRI G'AATT_C
Cuts at: 3557 5263 5641 3557
Size: 1706 378 5870
Fragments arranged by size:
5870 1706 378
EcoRV GAT'ATC
Cuts at: 3450 3450
Size: 7954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3353 3353
Size: 7954
FspI TGC'GCA
Cuts at: 2868 6460 2868
Size: 3592 4362
HaeII r_GCGC'y
Cuts at: 1678 2259 2858 4746 7101 1678
Size: 581 599 1888 2355 2531
Fragments arranged by size:
2531 2355 1888 599 581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 4089 4121 4919
Size: 32 135 32 1550 32 935 32 798
Cuts at: 4919 4951 5055 5087 6595 6627 6669 6701 1373
Size: 32 104 32 1508 32 42 32 2626
Fragments arranged by size:
2626 1550 1508 935 798 135 104 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 5298 5544 669
Size: 988 281 1467 1893 246 3079
Fragments arranged by size:
3079 1893 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2657 748
Size: 1909 6045
HpaI GTT'AAC
Cuts at: 5544 5544
Size: 7954
KpnI G_GTAC'C
Cuts at: 3326 3434 5279 3326
Size: 108 1845 6001
Fragments arranged by size:
6001 1845 108
MfeI C'AATT_G
Cuts at: 5553 5553
Size: 7954
MluI A'CGCG_T
Cuts at: 7830 7830
Size: 7954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 5148 7068 7252 1305
Size: 1074 2769 1920 184 2007
Fragments arranged by size:
2769 2007 1920 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6132
Fragments arranged by size:
6132 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3299 3779 4505 4670 5156 5912
Size: 648 2109 24 480 726 165 486 756
Cuts at: 5912 6271 6430 518
Size: 359 159 2042
Fragments arranged by size:
2109 2042 756 726 648 486 480 359
165 159 24
MspA1I CmG'CkG
Cuts at: 1606 3988 4027 5237 5370 6047 7195 7440 1606
Size: 2382 39 1210 133 677 1148 245 2120
Fragments arranged by size:
2382 2120 1210 1148 677 245 133 39
NcoI C'CATG_G
Cuts at: 513 1065 3276 4282 513
Size: 552 2211 1006 4185
Fragments arranged by size:
4185 2211 1006 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7954
NheI G'CTAG_C
Cuts at: 1052 2567 1052
Size: 1515 6439
NotI GC'GGCC_GC
Cuts at: 5401 5401
Size: 7954
NsiI A_TGCA'T
Cuts at: 3661 3776 3661
Size: 115 7839
NspI r_CATG'y
Cuts at: 1132 1229 2424 3663 3953 4504 5312 6857 1132
Size: 97 1195 1239 290 551 808 1545 2229
Fragments arranged by size:
2229 1545 1239 1195 808 551 290 97
PacI TTA_AT'TAA
Cuts at: 2370 2370
Size: 7954
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7954
PciI A'CATG_T
Cuts at: 3949 6853 3949
Size: 2904 5050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4288 4771 1108
Size: 3180 483 4291
Fragments arranged by size:
4291 3180 483
PmeI GTTT'AAAC
Cuts at: 5258 5258
Size: 7954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2943 6023 6055 7536 7568 2911
Size: 32 3080 32 1481 32 3297
Fragments arranged by size:
3297 3080 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4927 4927
Size: 7954
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7954
PsiI TTA'TAA
Cuts at: 5524 5524
Size: 7954
PspOMI G'GGCC_C
Cuts at: 2997 2997
Size: 7954
PspXI vC'TCGA_Gb
Cuts at: 4850 4850
Size: 7954
PstI C_TGCA'G
Cuts at: 830 3042 3993 4969 5306 830
Size: 2212 951 976 337 3478
Fragments arranged by size:
3478 2212 976 951 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7954
SalI G'TCGA_C
Cuts at: 1936 5296 1936
Size: 3360 4594
SapI GCTCTTCn'nnn_
Cuts at: 3618 4619 3618
Size: 1001 6953
SbfI CC_TGCA'GG
Cuts at: 5306 5306
Size: 7954
ScaI AGT'ACT
Cuts at: 6202 7914 6202
Size: 1712 6242
SexAI A'CCwGG_T
Cuts at: 3234 3234
Size: 7954
SfcI C'TryA_G
Cuts at: 826 1045 2179 2700 3038 3343 3989 4780 4965
Size: 219 1134 521 338 305 646 791 185
Cuts at: 4965 5302 6437 7118 7309 826
Size: 337 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 791 681 646 521 338
337 305 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7954
SmaI CCC'GGG
Cuts at: 1872 5281 1872
Size: 3409 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2691 3139 4850 5129 6058 6959
Size: 197 926 748 448 1711 279 929 901
Cuts at: 6959 7221 820
Size: 262 1553
Fragments arranged by size:
1711 1553 929 926 901 748 448 279
262 197
SnaBI TAC'GTA
Cuts at: 493 5745 493
Size: 5252 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7954
SphI G_CATG'C
Cuts at: 1132 3663 5312 1132
Size: 2531 1649 3774
Fragments arranged by size:
3774 2531 1649
SspI AAT'ATT
Cuts at: 5 52 2201 2678 5878 5
Size: 47 2149 477 3200 2081
Fragments arranged by size:
3200 2149 2081 477 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7954
StyI C'CwwG_G
Cuts at: 513 1065 2040 2050 3276 4282 5393 513
Size: 552 975 10 1226 1006 1111 3074
Fragments arranged by size:
3074 1226 1111 1006 975 552 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5981 5998 6151 6336 6781 325
Size: 1255 4401 17 153 185 445 1498
Fragments arranged by size:
4401 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6200 7912 96
Size: 274 80 33 51 1443 4223 1712 138
Fragments arranged by size:
4223 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 7914 7946 4568
Size: 32 3314 32 4576
Fragments arranged by size:
4576 3314 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7954
XbaI T'CTAG_A
Cuts at: 5290 5290
Size: 7954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4003 4561 4003
Size: 558 7396
XhoI C'TCGA_G
Cuts at: 1943 2691 4850 1943
Size: 748 2159 5047
Fragments arranged by size:
5047 2159 748
XmaI C'CCGG_G
Cuts at: 1870 5279 1870
Size: 3409 4545
XmnI GAAnn'nnTTC
Cuts at: 4398 6083 4398
Size: 1685 6269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5762 276
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PfoI PmlI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI