Restriction map for
pFN21AA0006
(Circular) (Six-base) MAPSORT of: pFN21AA0006.seq Check: 4821 from: 1 to: 7033
pFN21AA0006 7033 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2962 3784 4376 1937
Size: 1025 822 592 4594
Fragments arranged by size:
4594 1025 822 592
Acc65I G'GTAC_C
Cuts at: 3411 4354 3411
Size: 943 6090
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 2768 3006 4208 5096 6480 6748 1632
Size: 579 557 238 1202 888 1384 268 1917
Fragments arranged by size:
1917 1384 1202 888 579 557 268 238
AflII C'TTAA_G
Cuts at: 820 1017 2806 820
Size: 197 1789 5047
Fragments arranged by size:
5047 1789 197
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AlwNI CAG_nnn'CTG
Cuts at: 3298 4776 6348 3298
Size: 1478 1572 3983
Fragments arranged by size:
3983 1572 1478
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 3887 4015 4342 4568 4720 6829 1493
Size: 2394 128 327 226 152 2109 1697
Fragments arranged by size:
2394 2109 1697 327 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 3732 4358 1870
Size: 73 1789 626 4545
Fragments arranged by size:
4545 1789 626 73
BamHI G'GATC_C
Cuts at: 2179 4363 4730 2179
Size: 2184 367 4482
Fragments arranged by size:
4482 2184 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3411 4354 5809 618
Size: 325 217 627 1624 943 1455 1842
Fragments arranged by size:
1842 1624 1455 943 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3095 4352 1391
Size: 1704 1257 4072
Fragments arranged by size:
4072 1704 1257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3911 928
Size: 971 2012 4050
Fragments arranged by size:
4050 2012 971
BbvCI CC'TCA_GC
Cuts at: 3122 3122
Size: 7033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3110 3184 4925 6135 3110
Size: 74 1741 1210 4008
Fragments arranged by size:
4008 1741 1210 74
BclI T'GATC_A
Cuts at: 1548 2136 3642 1548
Size: 588 1506 4939
Fragments arranged by size:
4939 1506 588
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3262 7028 3262
Size: 3766 3267
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 5094 6250 1790
Size: 3304 1156 2573
Fragments arranged by size:
3304 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 2870 4764
Size: 416 53 83 399 24 1211 242 1894
Cuts at: 4764 5722 442
Size: 958 1753
Fragments arranged by size:
1894 1753 1211 958 416 399 242 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3164 3305 5693 1239
Size: 1925 141 2388 2579
Fragments arranged by size:
2579 2388 1925 141
Bpu10I CC'TnA_GC
Cuts at: 3122 3122
Size: 7033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 5158 6023 6321 4021
Size: 1137 865 298 4733
Fragments arranged by size:
4733 1137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2662 5696 882
Size: 617 1163 3034 2219
Fragments arranged by size:
3034 2219 1163 617
BsaAI yAC'GTr
Cuts at: 493 1204 4824 493
Size: 711 3620 2702
Fragments arranged by size:
3620 2702 711
BsaBI GATnn'nnATC
Cuts at: 3195 7027 3195
Size: 3832 3201
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4840 5222 5857 275
Size: 53 83 186 936 3307 382 635 1451
Fragments arranged by size:
3307 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2083 3722 5466 5864 6138 6285 6696 2083
Size: 1639 1744 398 274 147 411 2420
Fragments arranged by size:
2420 1744 1639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3763 3793 1178
Size: 30 165 30 2360 30 4418
Fragments arranged by size:
4418 2360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 3419 3741 3941 1186
Size: 916 1317 322 200 4278
Fragments arranged by size:
4278 1317 916 322 200
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3398 3529 3777 3988 1163
Size: 573 1662 131 248 211 4208
Fragments arranged by size:
4208 1662 573 248 211 131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 5244 6272 1540
Size: 275 186 2482 761 1028 2301
Fragments arranged by size:
2482 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3039 3703 5094 5179 6250 3039
Size: 664 1391 85 1071 3822
Fragments arranged by size:
3822 1391 1071 664 85
BsmI GAATG_Cn'
Cuts at: 3233 4544 4637 3233
Size: 1311 93 5629
Fragments arranged by size:
5629 1311 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3039 3095 3703 4352 5094 5179 6250
Size: 399 1249 56 608 649 742 85 1071
Cuts at: 6250 1391
Size: 2174
Fragments arranged by size:
2174 1249 1071 742 649 608 399 85
56
BspEI T'CCGG_A
Cuts at: 2083 6696 2083
Size: 4613 2420
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3661 3799 4388 844
Size: 2817 138 589 3489
Fragments arranged by size:
3489 2817 589 138
BsrBI CCG'CTC
Cuts at: 1394 1823 3409 4920 1394
Size: 429 1586 1511 3507
Fragments arranged by size:
3507 1586 1511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3260 4220 5528 5702 65
Size: 1183 2012 960 1308 174 1396
Fragments arranged by size:
2012 1396 1308 1183 960 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 2574 5093 6105 2574
Size: 2519 1012 3502
Fragments arranged by size:
3502 2519 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7033
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 2179 3262 4363 4730 5115 5132 6584 6670
Size: 207 1083 1101 367 385 17 1452 86
Cuts at: 6670 6682 7028 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1101 1083 385 367 346 207
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4077 4077
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 3767 4030 513
Size: 552 2702 263 3516
Fragments arranged by size:
3516 2702 552 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 4549 5343 5363 4549
Size: 794 20 6219
Fragments arranged by size:
6219 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2442 2477 529
Size: 35 1878 35 5085
Fragments arranged by size:
5085 1878 35 35
DraI TTT'AAA
Cuts at: 2097 2986 4337 2097
Size: 889 1351 4793
Fragments arranged by size:
4793 1351 889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4117 4480 5369
Size: 54 1425 50 275 18 2287 363 889
Cuts at: 5369 6998 8
Size: 1629 43
Fragments arranged by size:
2287 1629 1425 889 363 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2377 2796 3266 3960 4077 4963 1353
Size: 1024 419 470 694 117 886 3423
Fragments arranged by size:
3423 1024 886 694 470 419 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2768 3006 3164 3305 4208 5096
Size: 393 579 557 238 158 141 903 888
Cuts at: 5096 5693 6480 6748 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 903 888 787 597 579 557 393
268 238 158 141
EcoO109I rG'GnC_Cy
Cuts at: 3650 3737 4246 3650
Size: 87 509 6437
Fragments arranged by size:
6437 509 87
EcoRI G'AATT_C
Cuts at: 4342 4720 4342
Size: 378 6655
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 2525 2557 2837
Size: 32 135 32 758 32 163 32 280
Cuts at: 2837 2869 3824 3856 5674 5706 5748 5780 1373
Size: 32 955 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 955 758 280 163 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2323 4377 4623 669
Size: 988 281 385 2054 246 3079
Fragments arranged by size:
3079 2054 988 385 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 3415 4358 3415
Size: 943 6090
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3039 3603 6147 6331 1305
Size: 1734 564 2544 184 2007
Fragments arranged by size:
2544 2007 1734 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 4991 5350 5509 518
Size: 648 1885 1940 359 159 2042
Fragments arranged by size:
2042 1940 1885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3026 3283 3631 3956 4449 5126 6274 6519
Size: 1420 257 348 325 493 677 1148 245
Cuts at: 6519 1606
Size: 2120
Fragments arranged by size:
2120 1420 1148 677 493 348 325 257
245
NcoI C'CATG_G
Cuts at: 513 1065 4030 513
Size: 552 2965 3516
Fragments arranged by size:
3516 2965 552
NdeI CA'TA_TG
Cuts at: 387 3183 387
Size: 2796 4237
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 3393 4391 5936 1132
Size: 97 2164 998 1545 2229
Fragments arranged by size:
2229 2164 1545 998 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2911 1108
Size: 1803 5230
PfoI T'CCnGG_A
Cuts at: 2751 3612 2751
Size: 861 6172
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3650 3737 4246 3650
Size: 87 509 6437
Fragments arranged by size:
6437 509 87
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 3761 3828 4603 3761
Size: 67 775 6191
Fragments arranged by size:
6191 775 67
PstI C_TGCA'G
Cuts at: 830 2820 4385 830
Size: 1990 1565 3478
Fragments arranged by size:
3478 1990 1565
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 3026 3283 3631 3956 3026
Size: 257 348 325 6103
Fragments arranged by size:
6103 348 325 257
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 2308 2839 4042 5281 6993 2308
Size: 531 1203 1239 1712 2348
Fragments arranged by size:
2348 1712 1239 1203 531
SfcI C'TryA_G
Cuts at: 826 1045 2816 4381 5516 6197 6388 826
Size: 219 1771 1565 1135 681 191 1471
Fragments arranged by size:
1771 1565 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2806 4036 5137 6038 6300 820
Size: 197 926 863 1230 1101 901 262 1553
Fragments arranged by size:
1553 1230 1101 926 901 863 262 197
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 2256 4957 5
Size: 47 2204 2701 2081
Fragments arranged by size:
2701 2204 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2295 2910 3741 4030 4472 513
Size: 552 1230 615 831 289 442 3074
Fragments arranged by size:
3074 1230 831 615 552 442 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5060 5077 5230 5415 5860 325
Size: 1255 3480 17 153 185 445 1498
Fragments arranged by size:
3480 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2306 2837 3950
Size: 274 80 33 51 1443 329 531 1113
Cuts at: 3950 4040 5279 6991 96
Size: 90 1239 1712 138
Fragments arranged by size:
1712 1443 1239 1113 531 329 274 138
90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3495 3527 6993 7025 3495
Size: 32 3466 32 3503
Fragments arranged by size:
3503 3466 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4175 1316
Size: 2859 4174
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 3309 4051 5162 3309
Size: 742 1111 5180
Fragments arranged by size:
5180 1111 742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI