Restriction map for
pFN21AA0005
(Circular) (Six-base) MAPSORT of: pFN21AA0005.seq Check: 1256 from: 1 to: 6058
pFN21AA0005 6058 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3149 3228 3342 3360 3366 4121 5505 5773
Size: 1517 79 114 18 6 755 1384 268
Cuts at: 5773 1632
Size: 1917
Fragments arranged by size:
1917 1517 1384 755 268 114 79 18
6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 3253 4957 5934 1203
Size: 2050 1704 977 1327
Fragments arranged by size:
2050 1704 1327 977
AgeI A'CCGG_T
Cuts at: 2228 4889 2228
Size: 2661 3397
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2445 1184
Size: 1261 4797
AlwNI CAG_nnn'CTG
Cuts at: 2048 2708 3801 5373 2048
Size: 660 1093 1572 2733
Fragments arranged by size:
2733 1572 1093 660
ApaI G_GGCC'C
Cuts at: 2026 2026
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2623 3367 3593 3745 5854 1493
Size: 1130 744 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 1870
Size: 73 1440 4545
Fragments arranged by size:
4545 1440 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 3379 4834 618
Size: 325 217 627 227 1365 1455 1842
Fragments arranged by size:
1842 1455 1365 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2026 2860 2871 3228 3377 1391
Size: 635 834 11 357 149 4072
Fragments arranged by size:
4072 834 635 357 149 11
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 6058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 928
Size: 971 809 4278
Fragments arranged by size:
4278 971 809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BfrBI ATG'CAT
Cuts at: 2594 2594
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2407 6053 2407
Size: 3646 2412
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 2026 4119 5275 1790
Size: 236 2093 1156 2573
Fragments arranged by size:
2573 2093 1156 236
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3789 4747 442
Size: 416 53 83 399 24 2372 958 1753
Fragments arranged by size:
2372 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2552 1056
Size: 1496 4562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 2492 2855 4718 1239
Size: 1053 200 363 1863 2579
Fragments arranged by size:
2579 1863 1053 363 200
Bpu10I CC'TnA_GC
Cuts at: 2433 3215 2433
Size: 782 5276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3084 3302 4183 5048 5346 3084
Size: 218 881 865 298 3796
Fragments arranged by size:
3796 881 865 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 4721 882
Size: 617 909 2313 2219
Fragments arranged by size:
2313 2219 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3865 4247 4882
Size: 53 83 186 936 482 1850 382 635
Cuts at: 4882 275
Size: 1451
Fragments arranged by size:
1850 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2228 4491 4889 5163 5310 5721 2228
Size: 2263 398 274 147 411 2565
Fragments arranged by size:
2565 2263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3044 3236 1186
Size: 1768 90 192 4008
Fragments arranged by size:
4008 1768 192 90
BseYI C'CCAG_C
Cuts at: 5261 5757 5261
Size: 496 5562
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 3508 4269 5297 1540
Size: 275 186 71 1436 761 1028 2301
Fragments arranged by size:
2301 1436 1028 761 275 186 71
BsiHKAI G_wGCw'C
Cuts at: 2860 2871 3008 4119 4204 5275 2860
Size: 11 137 1111 85 1071 3643
Fragments arranged by size:
3643 1111 1071 137 85 11
BsmI GAATG_Cn'
Cuts at: 2340 3569 3662 2340
Size: 1229 93 4736
Fragments arranged by size:
4736 1229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2860 2871 3008 3228 3377 4119
Size: 399 236 834 11 137 220 149 742
Cuts at: 4119 4204 5275 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 834 742 399 236 220 149
137 85 11
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3165 3947 5677 3165
Size: 782 1730 3546
Fragments arranged by size:
3546 1730 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3413 844
Size: 2569 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 3945 1394
Size: 429 235 1887 3507
Fragments arranged by size:
3507 1887 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 4702 4889 1700
Size: 110 418 2474 187 2869
Fragments arranged by size:
2869 2474 418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2654 2708 3257 794
Size: 1860 54 549 3595
Fragments arranged by size:
3595 1860 549 54
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 2407 3388 3755 4140 4157 5609 5695 5707
Size: 435 981 367 385 17 1452 86 12
Cuts at: 5707 6053 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 435 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2899 513
Size: 552 1834 3672
Fragments arranged by size:
3672 1834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 4368 4388 3574
Size: 794 20 5244
Fragments arranged by size:
5244 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 2149 2671 3362 2149
Size: 522 691 4845
Fragments arranged by size:
4845 691 522
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2092 5065 809
Size: 507 776 2973 1802
Fragments arranged by size:
2973 1802 776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 3505 4394
Size: 54 1425 50 275 18 239 1436 889
Cuts at: 4394 6023 8
Size: 1629 43
Fragments arranged by size:
1629 1436 1425 889 275 239 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2069 3505 1537
Size: 275 257 1436 4090
Fragments arranged by size:
4090 1436 275 257
EarI CTCTTCn'nnn_
Cuts at: 1353 3263 3988 1353
Size: 1910 725 3423
Fragments arranged by size:
3423 1910 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2858 2869 2858
Size: 11 6047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2492 2855 3149 3228 3342 3360
Size: 393 660 200 363 294 79 114 18
Cuts at: 3360 3366 4121 4718 5505 5773 1239
Size: 6 755 597 787 268 1524
Fragments arranged by size:
1524 787 755 660 597 393 363 294
268 200 114 79 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3218
Size: 6058
EcoO109I rG'GnC_Cy
Cuts at: 2022 2023 2022
Size: 1 6057
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
EcoRV GAT'ATC
Cuts at: 2695 2695
Size: 6058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2018 2040 2461 5205 1678
Size: 340 22 421 2744 2531
Fragments arranged by size:
2744 2531 421 340 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373
Size: 32 135 32 3127 32 42 32 2626
Fragments arranged by size:
3127 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 3402 3648 669
Size: 988 281 621 843 246 3079
Fragments arranged by size:
3079 988 843 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 5172 5356 1305
Size: 1209 2658 184 2007
Fragments arranged by size:
2658 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4236
Fragments arranged by size:
4236 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 4016 4375 4534 518
Size: 648 1155 1695 359 159 2042
Fragments arranged by size:
2042 1695 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3474 4151 5299 5544 1606
Size: 1868 677 1148 245 2120
Fragments arranged by size:
2120 1868 1148 677 245
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 6058
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5506
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 2548 1052
Size: 1496 4562
NotI GC'GGCC_GC
Cuts at: 2069 3505 2069
Size: 1436 4622
NsiI A_TGCA'T
Cuts at: 2596 2596
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3257 3416 4961 1132
Size: 97 2028 159 1545 2229
Fragments arranged by size:
2229 2028 1545 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 3253 4957 3253
Size: 1704 4354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2051 1613
Size: 438 5620
PsiI TTA'TAA
Cuts at: 2616 3201 3628 2616
Size: 585 427 5046
Fragments arranged by size:
5046 585 427
PspOMI G'GGCC_C
Cuts at: 2022 2022
Size: 6058
PstI C_TGCA'G
Cuts at: 830 3410 830
Size: 2580 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
SacI G_AGCT'C
Cuts at: 2860 2871 2860
Size: 11 6047
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 3406 4541 5222 5413 826
Size: 219 2361 1135 681 191 1471
Fragments arranged by size:
2361 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 6058
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 3385 1872
Size: 1513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3099 3281 4162 5063 5325 820
Size: 197 926 1156 182 881 901 262 1553
Fragments arranged by size:
1553 1156 926 901 881 262 197 182
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 2795 3982 5
Size: 47 2743 1187 2081
Fragments arranged by size:
2743 2081 1187 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 3497 513
Size: 552 2432 3074
Fragments arranged by size:
3074 2432 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 2410 3394 2410
Size: 984 5074
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 3383 1870
Size: 1513 4545
XmnI GAAnn'nnTTC
Cuts at: 2754 2832 3173 4187 2754
Size: 78 341 1014 4625
Fragments arranged by size:
4625 1014 341 78
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI