Restriction map for
pFN21AA0001
(Circular) (Six-base) MAPSORT of: pFN21AA0001.seq Check: 6456 from: 1 to: 5719
pFN21AA0001 5719 bp
With 182 enzymes: SgfI *
May 18, 2009 16:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2657 820
Size: 197 1640 3882
Fragments arranged by size:
3882 1640 197
AflIII A'CryG_T
Cuts at: 1203 2274 2316 4618 5595 1203
Size: 1071 42 2302 977 1327
Fragments arranged by size:
2302 1327 1071 977 42
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2884 1184
Size: 1700 4019
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 2671 2837 3028 3254 3406 5515 1493
Size: 1178 166 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 1178 226 191 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 1870
Size: 73 1101 4545
Fragments arranged by size:
4545 1101 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3003 3036 3003
Size: 33 5686
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3040 4495 618
Size: 325 217 627 1253 1455 1842
Fragments arranged by size:
1842 1455 1253 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2346 3038 1391
Size: 955 692 4072
Fragments arranged by size:
4072 955 692
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2092 928
Size: 971 193 4555
Fragments arranged by size:
4555 971 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 2007 2061 1548
Size: 459 54 5206
Fragments arranged by size:
5206 459 54
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2157 3780 4936 1790
Size: 367 1623 1156 2573
Fragments arranged by size:
2573 1623 1156 367
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 3450 4408
Size: 416 53 83 399 24 1132 901 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1132 958 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 4379 1239
Size: 777 2363 2579
Fragments arranged by size:
2579 2363 777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3844 4709 5007 2168
Size: 1676 865 298 2880
Fragments arranged by size:
2880 1676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 4382 882
Size: 617 797 2086 2219
Fragments arranged by size:
2219 2086 797 617
BsaAI yAC'GTr
Cuts at: 493 1204 2152 3510 493
Size: 711 948 1358 2702
Fragments arranged by size:
2702 1358 948 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2014 2044 1178
Size: 30 165 30 611 30 4853
Fragments arranged by size:
4853 611 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4461
BseYI C'CCAG_C
Cuts at: 2156 4922 5418 2156
Size: 2766 496 2457
Fragments arranged by size:
2766 2457 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 1163
Size: 573 569 4577
Fragments arranged by size:
4577 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 3780 3865 4936 2304
Size: 1476 85 1071 3087
Fragments arranged by size:
3087 1476 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2304 2346 3038 3780 3865 4936
Size: 399 367 147 42 692 742 85 1071
Cuts at: 4936 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 692 399 367 147 85
42
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 4214 4388 65
Size: 1183 855 2111 174 1396
Fragments arranged by size:
2111 1396 1183 855 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2235 3049 3416 3801 3818 5270 5356 5368
Size: 263 814 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 814 385 367 346 263 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 3166 4055
Size: 54 1425 50 275 18 750 586 889
Cuts at: 4055 5684 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 586 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 3649 1353
Size: 956 1340 3423
Fragments arranged by size:
3423 1340 956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 3782 4379 5166 5434 1239
Size: 393 384 1766 597 787 268 1524
Fragments arranged by size:
1766 1524 787 597 393 384 268
EcoRI G'AATT_C
Cuts at: 2671 2837 3028 3406 2671
Size: 166 191 378 4984
Fragments arranged by size:
4984 378 191 166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 4866 1678
Size: 3188 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2776 2808 4360 4392 4434
Size: 32 135 32 1204 32 1552 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1552 1204 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2314 3063 3309 669
Size: 988 281 376 749 246 3079
Fragments arranged by size:
3079 988 749 376 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2648 4833 5017 1305
Size: 1343 2185 184 2007
Fragments arranged by size:
2185 2007 1343 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 3147
Fragments arranged by size:
3147 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2576 3677 4036 4195 518
Size: 648 915 495 1101 359 159 2042
Fragments arranged by size:
2042 1101 915 648 495 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2802 3135 3812 4960 5205 1606
Size: 663 533 333 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 533 333 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2320 3077 4622 1132
Size: 97 1091 757 1545 2229
Fragments arranged by size:
2229 1545 1091 757 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 2316 4618 2316
Size: 2302 3417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2589 1108
Size: 1481 4238
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 2367 3289 2367
Size: 922 4797
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2269 2802 2269
Size: 533 5186
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2309 2309
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 3067 4202 4883 5074 826
Size: 219 2022 1135 681 191 1471
Fragments arranged by size:
2022 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2657 3823 4724 4986 820
Size: 197 926 240 474 1166 901 262 1553
Fragments arranged by size:
1553 1166 926 901 474 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 2473 3643 5
Size: 47 2421 1170 2081
Fragments arranged by size:
2421 2081 1170 47
StuI AGG'CCT
Cuts at: 1517 2253 1517
Size: 736 4983
StyI C'CwwG_G
Cuts at: 513 1065 2239 2254 3158 513
Size: 552 1174 15 904 3074
Fragments arranged by size:
3074 1174 904 552 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2671 2801 3746 3763 3916 4101 4546
Size: 1255 1091 130 945 17 153 185 445
Cuts at: 4546 325
Size: 1498
Fragments arranged by size:
1498 1255 1091 945 445 185 153 130
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 3965 5677
Size: 274 80 33 51 1443 111 1877 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1877 1712 1443 274 138 111 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2014 2046 5679 5711 2014
Size: 32 3633 32 2022
Fragments arranged by size:
3633 2022 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 2837 3848 2837
Size: 1011 4708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI