Result of FASTA (omim) for
pFN21AE5409
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE5409, 349 aa
1>>>pF1KE5409 349 - 349 aa - 349 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 6.0207+/-0.000373; mu= 13.1887+/- 0.023
mean_var=83.1375+/-16.720, 0's: 0 Z-trim(114.6): 39 B-trim: 357 in 1/55
Lambda= 0.140662
statistics sampled from 24511 (24549) to 24511 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.652), E-opt: 0.2 (0.288), width: 16
Scan time: 7.680
The best scores are: opt bits E(85289)
NP_631908 (OMIM: 610726) probable tRNA pseudouridi ( 349) 2208 457.8 1.6e-128
NP_001316790 (OMIM: 610727) probable tRNA pseudour ( 287) 420 94.9 2.3e-19
NP_001316791 (OMIM: 610727) probable tRNA pseudour ( 275) 382 87.2 4.7e-17
NP_056494 (OMIM: 610727) probable tRNA pseudouridi ( 331) 382 87.2 5.5e-17
NP_001316792 (OMIM: 610727) probable tRNA pseudour ( 227) 326 75.8 1.1e-13
>>NP_631908 (OMIM: 610726) probable tRNA pseudouridine s (349 aa)
initn: 2208 init1: 2208 opt: 2208 Z-score: 2428.2 bits: 457.8 E(85289): 1.6e-128
Smith-Waterman score: 2208; 100.0% identity (100.0% similar) in 349 aa overlap (1-349:1-349)
10 20 30 40 50 60
pF1KE5 MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAA
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE5 LATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 LATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTL
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE5 DSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 DSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQE
130 140 150 160 170 180
190 200 210 220 230 240
pF1KE5 DIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 DIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQP
190 200 210 220 230 240
250 260 270 280 290 300
pF1KE5 PFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDI
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 PFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDI
250 260 270 280 290 300
310 320 330 340
pF1KE5 AQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC
:::::::::::::::::::::::::::::::::::::::::::::::::
NP_631 AQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC
310 320 330 340
>>NP_001316790 (OMIM: 610727) probable tRNA pseudouridin (287 aa)
initn: 256 init1: 146 opt: 420 Z-score: 468.5 bits: 94.9 E(85289): 2.3e-19
Smith-Waterman score: 420; 33.3% identity (59.3% similar) in 270 aa overlap (65-330:6-265)
40 50 60 70 80 90
pF1KE5 PSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLL
: : :.:::.:: : .: . .. .::
NP_001 MGSAGLSRLHGLFAVYKPPGLKWKHLRDTVELQLL
10 20 30
100 110 120 130 140 150
pF1KE5 AEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSG--SKRYTAI
: : ... ::.: : ::. : ::::.:.: : ..::.: .. .: ::.
NP_001 KVCG---PAFAH-----LKVGVGHRLDAQASGVLVLGVGHGCRLLTDMYNAHLTKDYTVR
40 50 60 70 80
160 170 180 190 200 210
pF1KE5 GELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLST
: :::::: . ::..:. ::..:.: .. :: . :. ... .:: : :.
NP_001 GLLGKATDDFREDGRLVEKTTYDHVTREKLDRILAVIQGSHQKALVMYSNLDLKTQEAYE
90 100 110 120 130 140
220 230 240 250 260 270
pF1KE5 LMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFY--IRSLVSDIGKELSSCA
. :: ... :. . .: : :: : :.:.: .:.:: .:: ::.. :
NP_001 MAVRG-LIRPMNKSPMLITGIRCLYFAPPEFLLEVQCMHETQKELRKLVHEIGLELKTTA
150 160 170 180 190 200
280 290 300 310 320 330
pF1KE5 NVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLS
.. ::..: :::. :: . .: . .: .... . ::: . : ...:. :
NP_001 VCTQVRRTRDGFFTLDS-ALLRTQWDLTNIQDAIRAATPQVAAELEKSLSPGLDTKQLPS
210 220 230 240 250 260
340
pF1KE5 CEYITLNEPKREDDVIKTC
NP_001 PGWSWDSQGPSSTLGLERGAGQ
270 280
>>NP_001316791 (OMIM: 610727) probable tRNA pseudouridin (275 aa)
initn: 206 init1: 146 opt: 382 Z-score: 427.1 bits: 87.2 E(85289): 4.7e-17
Smith-Waterman score: 382; 34.1% identity (61.0% similar) in 223 aa overlap (112-330:33-253)
90 100 110 120 130 140
pF1KE5 SAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTS
::.: : ::. : ::::.:.: : ..::.
NP_001 GSEEKELTLTATSVPSFINHPLVCGPAFAHLKVGVGHRLDAQASGVLVLGVGHGCRLLTD
10 20 30 40 50 60
150 160 170 180 190
pF1KE5 MLSG--SKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPL
: .. .: ::. : :::::: . ::..:. ::..:.: .. :: . :. ... .
NP_001 MYNAHLTKDYTVRGLLGKATDDFREDGRLVEKTTYDHVTREKLDRILAVIQGSHQKALVM
70 80 90 100 110 120
200 210 220 230 240 250
pF1KE5 YSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFY--IRS
:: : :. . :: ... :. . .: : :: : :.:.: .:.
NP_001 YSNLDLKTQEAYEMAVRG-LIRPMNKSPMLITGIRCLYFAPPEFLLEVQCMHETQKELRK
130 140 150 160 170 180
260 270 280 290 300 310
pF1KE5 LVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELAL
:: .:: ::.. : .. ::..: :::. :: . .: . .: .... . :::
NP_001 LVHEIGLELKTTAVCTQVRRTRDGFFTLDS-ALLRTQWDLTNIQDAIRAATPQVAAELEK
190 200 210 220 230 240
320 330 340
pF1KE5 KKSKPESNEQVLSCEYITLNEPKREDDVIKTC
. : ...:. :
NP_001 SLSPGLDTKQLPSPGWSWDSQGPSSTLGLERGAGQ
250 260 270
>>NP_056494 (OMIM: 610727) probable tRNA pseudouridine s (331 aa)
initn: 253 init1: 146 opt: 382 Z-score: 425.9 bits: 87.2 E(85289): 5.5e-17
Smith-Waterman score: 382; 34.1% identity (61.0% similar) in 223 aa overlap (112-330:89-309)
90 100 110 120 130 140
pF1KE5 SAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTS
::.: : ::. : ::::.:.: : ..::.
NP_056 GSEEKELTLTATSVPSFINHPLVCGPAFAHLKVGVGHRLDAQASGVLVLGVGHGCRLLTD
60 70 80 90 100 110
150 160 170 180 190
pF1KE5 MLSG--SKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPL
: .. .: ::. : :::::: . ::..:. ::..:.: .. :: . :. ... .
NP_056 MYNAHLTKDYTVRGLLGKATDDFREDGRLVEKTTYDHVTREKLDRILAVIQGSHQKALVM
120 130 140 150 160 170
200 210 220 230 240 250
pF1KE5 YSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFY--IRS
:: : :. . :: ... :. . .: : :: : :.:.: .:.
NP_056 YSNLDLKTQEAYEMAVRG-LIRPMNKSPMLITGIRCLYFAPPEFLLEVQCMHETQKELRK
180 190 200 210 220 230
260 270 280 290 300 310
pF1KE5 LVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELAL
:: .:: ::.. : .. ::..: :::. :: . .: . .: .... . :::
NP_056 LVHEIGLELKTTAVCTQVRRTRDGFFTLDS-ALLRTQWDLTNIQDAIRAATPQVAAELEK
240 250 260 270 280 290
320 330 340
pF1KE5 KKSKPESNEQVLSCEYITLNEPKREDDVIKTC
. : ...:. :
NP_056 SLSPGLDTKQLPSPGWSWDSQGPSSTLGLERGAGQ
300 310 320 330
>>NP_001316792 (OMIM: 610727) probable tRNA pseudouridin (227 aa)
initn: 201 init1: 141 opt: 326 Z-score: 367.0 bits: 75.8 E(85289): 1.1e-13
Smith-Waterman score: 326; 31.9% identity (60.4% similar) in 207 aa overlap (128-330:1-205)
100 110 120 130 140 150
pF1KE5 GMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSG--SKRYTAIGEL
.:.:.: : ..::.: .. .: ::. : :
NP_001 MVLGVGHGCRLLTDMYNAHLTKDYTVRGLL
10 20 30
160 170 180 190 200 210
pF1KE5 GKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMK
::::: . ::..:. ::..:.: .. :: . :. ... .:: : :. .
NP_001 GKATDDFREDGRLVEKTTYDHVTREKLDRILAVIQGSHQKALVMYSNLDLKTQEAYEMAV
40 50 60 70 80 90
220 230 240 250 260 270
pF1KE5 RGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFY--IRSLVSDIGKELSSCANVL
:: ... :. . .: : :: : :.:.: .:.:: .:: ::.. :
NP_001 RG-LIRPMNKSPMLITGIRCLYFAPPEFLLEVQCMHETQKELRKLVHEIGLELKTTAVCT
100 110 120 130 140
280 290 300 310 320 330
pF1KE5 ELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEY
.. ::..: :::. :: . .: . .: .... . ::: . : ...:. :
NP_001 QVRRTRDGFFTLDS-ALLRTQWDLTNIQDAIRAATPQVAAELEKSLSPGLDTKQLPSPGW
150 160 170 180 190 200
340
pF1KE5 ITLNEPKREDDVIKTC
NP_001 SWDSQGPSSTLGLERGAGQ
210 220
349 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Tue Nov 8 00:27:49 2016 done: Tue Nov 8 00:27:50 2016
Total Scan time: 7.680 Total Display time: -0.010
Function used was FASTA [36.3.4 Apr, 2011]