Result of FASTA (omim) for
pFN21AE3984
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE3984, 204 aa
1>>>pF1KE3984 204 - 204 aa - 204 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 4.7325+/-0.000308; mu= 17.2554+/- 0.019
mean_var=72.2150+/-14.542, 0's: 0 Z-trim(118.9): 173 B-trim: 807 in 1/51
Lambda= 0.150925
statistics sampled from 32126 (32347) to 32126 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.765), E-opt: 0.2 (0.379), width: 16
Scan time: 5.880
The best scores are: opt bits E(85289)
NP_001654 (OMIM: 600464) ADP-ribosylation factor 6 ( 175) 337 81.7 8e-16
NP_001658 (OMIM: 601175) ADP-ribosylation factor-l ( 184) 323 78.6 6.9e-15
NP_001650 (OMIM: 103190) ADP-ribosylation factor 3 ( 181) 322 78.4 7.9e-15
XP_005268913 (OMIM: 103190) PREDICTED: ADP-ribosyl ( 181) 322 78.4 7.9e-15
NP_001019399 (OMIM: 103180) ADP-ribosylation facto ( 181) 315 76.9 2.3e-14
NP_001019398 (OMIM: 103180) ADP-ribosylation facto ( 181) 315 76.9 2.3e-14
NP_001019397 (OMIM: 103180) ADP-ribosylation facto ( 181) 315 76.9 2.3e-14
NP_001649 (OMIM: 103180) ADP-ribosylation factor 1 ( 181) 315 76.9 2.3e-14
NP_001653 (OMIM: 103188) ADP-ribosylation factor 5 ( 180) 314 76.7 2.6e-14
NP_004302 (OMIM: 604695) ADP-ribosylation factor-l ( 182) 314 76.7 2.6e-14
XP_016871749 (OMIM: 604695) PREDICTED: ADP-ribosyl ( 182) 314 76.7 2.6e-14
XP_005248554 (OMIM: 601747) PREDICTED: E3 ubiquiti ( 398) 314 77.0 4.6e-14
NP_079323 (OMIM: 614439) ADP-ribosylation factor-l ( 192) 310 75.8 5e-14
XP_016864933 (OMIM: 601747) PREDICTED: E3 ubiquiti ( 569) 314 77.1 6e-14
NP_150230 (OMIM: 601747) E3 ubiquitin-protein liga ( 569) 314 77.1 6e-14
NP_001647 (OMIM: 601747) E3 ubiquitin-protein liga ( 574) 314 77.1 6e-14
NP_001651 (OMIM: 601177) ADP-ribosylation factor 4 ( 180) 299 73.4 2.5e-13
NP_878899 (OMIM: 608922,612291) ADP-ribosylation f ( 428) 292 72.2 1.3e-12
NP_001167621 (OMIM: 608922,612291) ADP-ribosylatio ( 428) 292 72.2 1.3e-12
NP_060654 (OMIM: 616596) ADP-ribosylation factor-l ( 186) 286 70.6 1.8e-12
NP_620150 (OMIM: 616597) ADP-ribosylation factor-l ( 186) 282 69.7 3.4e-12
XP_011523084 (OMIM: 600732) PREDICTED: ADP-ribosyl ( 201) 281 69.5 4.1e-12
NP_001652 (OMIM: 600732) ADP-ribosylation factor-l ( 201) 281 69.5 4.1e-12
NP_150231 (OMIM: 601747) E3 ubiquitin-protein liga ( 546) 284 70.6 5.4e-12
NP_612459 (OMIM: 609351) ADP-ribosylation factor-l ( 196) 275 68.2 1e-11
XP_005266310 (OMIM: 609351) PREDICTED: ADP-ribosyl ( 196) 275 68.2 1e-11
XP_011510837 (OMIM: 608922,612291) PREDICTED: ADP- ( 403) 278 69.2 1.1e-11
NP_005729 (OMIM: 604786) ADP-ribosylation factor-l ( 200) 273 67.8 1.4e-11
NP_001032241 (OMIM: 604786) ADP-ribosylation facto ( 200) 273 67.8 1.4e-11
NP_001182325 (OMIM: 604786) ADP-ribosylation facto ( 200) 273 67.8 1.4e-11
NP_997625 (OMIM: 604786) ADP-ribosylation factor-l ( 200) 273 67.8 1.4e-11
NP_001168 (OMIM: 603425) ADP-ribosylation factor-l ( 181) 267 66.4 3.2e-11
NP_001310442 (OMIM: 209900,268000,600151,608845,61 ( 193) 264 65.8 5.2e-11
NP_005728 (OMIM: 604787) ADP-ribosylation factor-l ( 192) 262 65.4 7e-11
NP_001269360 (OMIM: 604787) ADP-ribosylation facto ( 201) 262 65.4 7.3e-11
XP_016862800 (OMIM: 209900,268000,600151,608845,61 ( 186) 256 64.0 1.7e-10
NP_115522 (OMIM: 209900,268000,600151,608845,61357 ( 186) 256 64.0 1.7e-10
NP_001265222 (OMIM: 209900,268000,600151,608845,61 ( 186) 256 64.0 1.7e-10
NP_816931 (OMIM: 209900,268000,600151,608845,61357 ( 186) 256 64.0 1.7e-10
XP_016856722 (OMIM: 103180) PREDICTED: ADP-ribosyl ( 212) 253 63.4 2.9e-10
XP_016862801 (OMIM: 209900,268000,600151,608845,61 ( 159) 251 62.9 3.2e-10
NP_001310443 (OMIM: 209900,268000,600151,608845,61 ( 159) 251 62.9 3.2e-10
XP_011526784 (OMIM: 604699) PREDICTED: ADP-ribosyl ( 201) 251 63.0 3.8e-10
NP_001254476 (OMIM: 604699) ADP-ribosylation facto ( 201) 251 63.0 3.8e-10
XP_016883065 (OMIM: 604699) PREDICTED: ADP-ribosyl ( 201) 251 63.0 3.8e-10
NP_001254477 (OMIM: 604699) ADP-ribosylation facto ( 201) 251 63.0 3.8e-10
NP_003215 (OMIM: 604699) ADP-ribosylation factor-r ( 201) 251 63.0 3.8e-10
XP_011526785 (OMIM: 604699) PREDICTED: ADP-ribosyl ( 201) 251 63.0 3.8e-10
XP_006719454 (OMIM: 103190) PREDICTED: ADP-ribosyl ( 137) 248 62.2 4.6e-10
NP_036229 (OMIM: 608960) ADP-ribosylation factor-l ( 179) 248 62.3 5.5e-10
>>NP_001654 (OMIM: 600464) ADP-ribosylation factor 6 [Ho (175 aa)
initn: 290 init1: 221 opt: 337 Z-score: 405.1 bits: 81.7 E(85289): 8e-16
Smith-Waterman score: 337; 35.2% identity (69.2% similar) in 159 aa overlap (32-190:13-169)
10 20 30 40 50 60
pF1KE3 SDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNVV
:. .. .:: ..:::..: :: . ...
NP_001 MGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTI
10 20 30 40
70 80 90 100 110 120
pF1KE3 STTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAARN
:.::....: ..:. .:: ..:: :.:: : .:: :.::.:::.: :. .: .. ::.
NP_001 PTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCAD-RDRIDEARQ
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 ELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDMD
::: .. .. .::.::.:: : : . .::.. . : . : . : .:: . :
NP_001 ELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRI-RDRNWYVQPSCATSGD
110 120 130 140 150 160
190 200
pF1KE3 ALKDSFSQLINLLEEKDHEAVRM
.: .... :
NP_001 GLYEGLTWLTSNYKS
170
>>NP_001658 (OMIM: 601175) ADP-ribosylation factor-like (184 aa)
initn: 307 init1: 201 opt: 323 Z-score: 388.3 bits: 78.6 E(85289): 6.9e-15
Smith-Waterman score: 323; 34.7% identity (68.9% similar) in 167 aa overlap (32-191:16-180)
10 20 30 40 50 60
pF1KE3 SDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNVV
: :. .:: ..:::..:.:. .:. :..
NP_001 MGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTIS
10 20 30 40
70 80 90 100 110 120
pF1KE3 STTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAARN
: ::.::.. .. ::. ..:: ..:.:: :.....:.:.:.:::. . .. .
NP_001 PTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQR-MQDCQR
50 60 70 80 90 100
130 140 150 160 170
pF1KE3 ELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCS-----
::.: : . .: .::.::.:: :.: : . :.. .::. . :...: .: ::
NP_001 ELQSLLVEERLAGATLLIFANKQDLPGALSSNAIREVLELDSI-RSHHWCIQGCSAVTGE
110 120 130 140 150 160
180 190 200
pF1KE3 --LDDMDALKDSFSQLINLLEEKDHEAVRM
: .: : :..:. :
NP_001 NLLPGIDWLLDDISSRIFTAD
170 180
>>NP_001650 (OMIM: 103190) ADP-ribosylation factor 3 [Ho (181 aa)
initn: 213 init1: 213 opt: 322 Z-score: 387.2 bits: 78.4 E(85289): 7.9e-15
Smith-Waterman score: 322; 33.9% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
NP_001 MGNIFGNLLKSLIGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
NP_001 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
NP_001 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :..:
NP_001 DGLYEGLDWLANQLKNKK
170 180
>>XP_005268913 (OMIM: 103190) PREDICTED: ADP-ribosylatio (181 aa)
initn: 213 init1: 213 opt: 322 Z-score: 387.2 bits: 78.4 E(85289): 7.9e-15
Smith-Waterman score: 322; 33.9% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
XP_005 MGNIFGNLLKSLIGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
XP_005 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
XP_005 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :..:
XP_005 DGLYEGLDWLANQLKNKK
170 180
>>NP_001019399 (OMIM: 103180) ADP-ribosylation factor 1 (181 aa)
initn: 213 init1: 213 opt: 315 Z-score: 379.0 bits: 76.9 E(85289): 2.3e-14
Smith-Waterman score: 315; 33.3% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
NP_001 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
NP_001 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
NP_001 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :...
NP_001 DGLYEGLDWLSNQLRNQK
170 180
>>NP_001019398 (OMIM: 103180) ADP-ribosylation factor 1 (181 aa)
initn: 213 init1: 213 opt: 315 Z-score: 379.0 bits: 76.9 E(85289): 2.3e-14
Smith-Waterman score: 315; 33.3% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
NP_001 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
NP_001 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
NP_001 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :...
NP_001 DGLYEGLDWLSNQLRNQK
170 180
>>NP_001019397 (OMIM: 103180) ADP-ribosylation factor 1 (181 aa)
initn: 213 init1: 213 opt: 315 Z-score: 379.0 bits: 76.9 E(85289): 2.3e-14
Smith-Waterman score: 315; 33.3% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
NP_001 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
NP_001 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
NP_001 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :...
NP_001 DGLYEGLDWLSNQLRNQK
170 180
>>NP_001649 (OMIM: 103180) ADP-ribosylation factor 1 [Ho (181 aa)
initn: 213 init1: 213 opt: 315 Z-score: 379.0 bits: 76.9 E(85289): 2.3e-14
Smith-Waterman score: 315; 33.3% identity (65.5% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. :. .. .:: ..:::..: :: ..
NP_001 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : .. ::
NP_001 IPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRER-VNEAR
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.:: : . .: .:..::.:: : : .. :: . :. : : . : .: .
NP_001 EELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL-RHRNWYIQATCATSG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
:.: .... : : :...
NP_001 DGLYEGLDWLSNQLRNQK
170 180
>>NP_001653 (OMIM: 103188) ADP-ribosylation factor 5 [Ho (180 aa)
initn: 287 init1: 202 opt: 314 Z-score: 377.8 bits: 76.7 E(85289): 2.6e-14
Smith-Waterman score: 314; 32.7% identity (66.1% similar) in 168 aa overlap (30-197:15-180)
10 20 30 40 50 60
pF1KE3 MSDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNV
. .. .. .:: ..:::..: :: ..
NP_001 MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTT
10 20 30 40
70 80 90 100 110 120
pF1KE3 VSTTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAAR
. : ::....: ..: ..: ..:: :.:: : .:.:..::.:::.:: . : :.:
NP_001 IPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESA-
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 NELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDM
.::.. ::. .: .:..::.:: : : :.:. . :. : :.. : .: .
NP_001 DELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHL-RSRTWYVQATCATQG
110 120 130 140 150 160
190 200
pF1KE3 DALKDSFSQLINLLEEKDHEAVRM
.: :... : . : ..
NP_001 TGLYDGLDWLSHELSKR
170 180
>>NP_004302 (OMIM: 604695) ADP-ribosylation factor-like (182 aa)
initn: 275 init1: 214 opt: 314 Z-score: 377.8 bits: 76.7 E(85289): 2.6e-14
Smith-Waterman score: 314; 35.0% identity (70.7% similar) in 157 aa overlap (32-188:17-171)
10 20 30 40 50 60
pF1KE3 SDLRITEAFLYMDYLCFRALCCKGPPPARPEYDLVCIGLTGSGKTSLLSKLCSESPDNVV
: .. .:: ..:::.::..: ::. ....
NP_004 MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHIT
10 20 30 40
70 80 90 100 110 120
pF1KE3 STTGFSIKAVPFQNAILNVKELGGADNIRKYWSRYYQGSQGVIFVLDSASSEDDLEAARN
: ::.::.: :. ::: ..:: .:: ::. :..... .:.:.:::. . .: . .
NP_004 PTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWKNYFENTDILIYVIDSADRKR-FEETGQ
50 60 70 80 90 100
130 140 150 160 170 180
pF1KE3 ELHSALQHPQLCTLPFLILANHQDKPAARSVQEIKKYFELEPLARGKRWILQPCSLDDMD
:: :.. .: .: ::.::.:: .: ..:: . ..:. . : . : .: :: .
NP_004 ELAELLEEEKLSCVPVLIFANKQDLLTAAPASEIAEGLNLHTI-RDRVWQIQSCSALTGE
110 120 130 140 150 160
190 200
pF1KE3 ALKDSFSQLINLLEEKDHEAVRM
...:...
NP_004 GVQDGMNWVCKNVNAKKK
170 180
204 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Sun Nov 6 08:21:18 2016 done: Sun Nov 6 08:21:19 2016
Total Scan time: 5.880 Total Display time: 0.000
Function used was FASTA [36.3.4 Apr, 2011]