Result of FASTA (omim) for
pFN21AE2524
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE2524, 457 aa
1>>>pF1KE2524 457 - 457 aa - 457 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 11.1948+/-0.000408; mu= -7.8084+/- 0.026
mean_var=376.6241+/-76.482, 0's: 0 Z-trim(123.2): 14 B-trim: 0 in 0/57
Lambda= 0.066088
statistics sampled from 42529 (42543) to 42529 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.783), E-opt: 0.2 (0.499), width: 16
Scan time: 11.220
The best scores are: opt bits E(85289)
NP_001268 (OMIM: 118491) choline kinase alpha isof ( 457) 3124 311.6 2.9e-84
NP_997634 (OMIM: 118491) choline kinase alpha isof ( 439) 1912 196.0 1.7e-49
XP_016872637 (OMIM: 118491) PREDICTED: choline kin ( 281) 1878 192.6 1.2e-48
XP_016872636 (OMIM: 118491) PREDICTED: choline kin ( 419) 1666 172.5 1.9e-42
NP_005189 (OMIM: 602541,612395) choline/ethanolami ( 395) 1227 130.6 7.2e-30
XP_016875069 (OMIM: 609858) PREDICTED: ethanolamin ( 425) 295 41.8 0.0043
>>NP_001268 (OMIM: 118491) choline kinase alpha isoform (457 aa)
initn: 3124 init1: 3124 opt: 3124 Z-score: 1633.9 bits: 311.6 E(85289): 2.9e-84
Smith-Waterman score: 3124; 100.0% identity (100.0% similar) in 457 aa overlap (1-457:1-457)
10 20 30 40 50 60
pF1KE2 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE2 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE2 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLE
130 140 150 160 170 180
190 200 210 220 230 240
pF1KE2 SVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 SVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM
190 200 210 220 230 240
250 260 270 280 290 300
pF1KE2 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
250 260 270 280 290 300
310 320 330 340 350 360
pF1KE2 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
310 320 330 340 350 360
370 380 390 400 410 420
pF1KE2 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
370 380 390 400 410 420
430 440 450
pF1KE2 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
:::::::::::::::::::::::::::::::::::::
NP_001 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
430 440 450
>>NP_997634 (OMIM: 118491) choline kinase alpha isoform (439 aa)
initn: 2988 init1: 1902 opt: 1912 Z-score: 1009.6 bits: 196.0 E(85289): 1.7e-49
Smith-Waterman score: 2956; 96.1% identity (96.1% similar) in 457 aa overlap (1-457:1-439)
10 20 30 40 50 60
pF1KE2 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE2 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE2 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLE
:::::::::::::::::::::::::::::::::: ::::::::
NP_997 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQM------------------GAEAMVLE
130 140 150 160
190 200 210 220 230 240
pF1KE2 SVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 SVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM
170 180 190 200 210 220
250 260 270 280 290 300
pF1KE2 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
230 240 250 260 270 280
310 320 330 340 350 360
pF1KE2 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
290 300 310 320 330 340
370 380 390 400 410 420
pF1KE2 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_997 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
350 360 370 380 390 400
430 440 450
pF1KE2 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
:::::::::::::::::::::::::::::::::::::
NP_997 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
410 420 430
>>XP_016872637 (OMIM: 118491) PREDICTED: choline kinase (281 aa)
initn: 1878 init1: 1878 opt: 1878 Z-score: 994.6 bits: 192.6 E(85289): 1.2e-48
Smith-Waterman score: 1878; 100.0% identity (100.0% similar) in 281 aa overlap (177-457:1-281)
150 160 170 180 190 200
pF1KE2 LYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLE
::::::::::::::::::::::::::::::
XP_016 MVLESVMFAILAERSLGPKLYGIFPQGRLE
10 20 30
210 220 230 240 250 260
pF1KE2 QFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 QFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFT
40 50 60 70 80 90
270 280 290 300 310 320
pF1KE2 EESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 EESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKL
100 110 120 130 140 150
330 340 350 360 370 380
pF1KE2 MLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAF
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 MLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAF
160 170 180 190 200 210
390 400 410 420 430 440
pF1KE2 QNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 QNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFD
220 230 240 250 260 270
450
pF1KE2 AYFHQKRKLGV
:::::::::::
XP_016 AYFHQKRKLGV
280
>>XP_016872636 (OMIM: 118491) PREDICTED: choline kinase (419 aa)
initn: 1663 init1: 1663 opt: 1666 Z-score: 883.1 bits: 172.5 E(85289): 1.9e-42
Smith-Waterman score: 2792; 91.7% identity (91.7% similar) in 457 aa overlap (1-457:1-419)
10 20 30 40 50 60
pF1KE2 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPP
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE2 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGL
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE2 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLE
::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 SNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQ--------
130 140 150 160 170
190 200 210 220 230 240
pF1KE2 SVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM
::::::::::::::::::::::::::::::
XP_016 ------------------------------SRRLDTEELSLPDISAEIAEKMATFHGMKM
180 190 200
250 260 270 280 290 300
pF1KE2 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV
210 220 230 240 250 260
310 320 330 340 350 360
pF1KE2 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYP
270 280 290 300 310 320
370 380 390 400 410 420
pF1KE2 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 FFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLW
330 340 350 360 370 380
430 440 450
pF1KE2 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
:::::::::::::::::::::::::::::::::::::
XP_016 GLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
390 400 410
>>NP_005189 (OMIM: 602541,612395) choline/ethanolamine k (395 aa)
initn: 1047 init1: 456 opt: 1227 Z-score: 657.2 bits: 130.6 E(85289): 7.2e-30
Smith-Waterman score: 1475; 57.9% identity (77.7% similar) in 385 aa overlap (74-455:29-388)
50 60 70 80 90 100
pF1KE2 ESKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTR---RRAYLWCKEFLP
: : .. .: :::: ::.:.:
NP_005 MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLG
10 20 30 40 50
110 120 130 140 150 160
pF1KE2 GAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKE
:::: .. .:... . :::::.::.::::: ..:.:::.:::::::::::
NP_005 GAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQ-------
60 70 80 90 100 110
170 180 190 200 210 220
pF1KE2 GSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELS
:....:::::::::::::::::.:::.::.:::::.:::: : :.::
NP_005 ------------GVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELR
120 130 140 150
230 240 250 260 270 280
pF1KE2 LPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSY
: .:: :: ::: ::::.:::.:::.:::::::.:::.. . : . ... : :
NP_005 EPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPT---GLPEMNLLEMY
160 170 180 190 200 210
290 300 310 320 330 340
pF1KE2 NLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRG
.: :. :::.::::::::::::::: :::::::: ::... :::.:::::::::::
NP_005 SLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADS--LMLVDFEYSSYNYRG
220 230 240 250 260 270
350 360 370 380 390 400
pF1KE2 FDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSI
::::::::::.:::..:..::..: :::..:::::: :: .. :.:: ::.
NP_005 FDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKG-ETLSQEEQRK
280 290 300 310 320 330
410 420 430 440 450
pF1KE2 IKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV
..:..:.::.:.::::::.::::::.::..:.:::::.::::.::. ::.:: .:
NP_005 LEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHS
340 350 360 370 380 390
NP_005 SS
>>XP_016875069 (OMIM: 609858) PREDICTED: ethanolamine ki (425 aa)
initn: 322 init1: 111 opt: 295 Z-score: 176.5 bits: 41.8 E(85289): 0.0043
Smith-Waterman score: 358; 26.9% identity (55.7% similar) in 368 aa overlap (65-425:96-415)
40 50 60 70 80 90
pF1KE2 QQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLW
.: :. : . ...: : : :. :
XP_016 LSAPAVLVVAVAVVVVVVSAVAWAMANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSL
70 80 90 100 110 120
100 110 120 130 140 150
pF1KE2 CKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQM
... : : .: .... :..: :. : . .: . ::.:.:: ..
XP_016 LQHLRPH-W---DPQEVTLQLFTDGITNKLIGCYVGNTMEDV------VLVRIYGNKTEL
130 140 150 160 170
160 170 180 190 200 210
pF1KE2 RSCNKEGSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRL
.....: .. : .: .. .:.:: : .: .:: .. :
XP_016 ---------LVDRDEEVKS----------FRVLQAHGCAPQLYCTFNNGLCYEFIQGEAL
180 190 200 210
220 230 240 250 260 270
pF1KE2 DTEELSLPDISAEIAEKMATFHGMKMPFNKEPK---WLFGTMEKYLKEVLRIKFTEESRI
: ... : : ::...: .:... . :: :: : ::.. .. :..:.
XP_016 DPKHVCNPAIFRLIARQLAKIHAIHAHNGWIPKSNLWL--KMGKYFS-LIPTGFADEDIN
220 230 240 250 260 270
280 290 300 310 320
pF1KE2 KKLHKLL--SYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLI
:.. . . : : :. .. .: . ::::.:::: ::. : .. ...:
XP_016 KRFLSDIPSSQILQEEMTWMKEILSNLGSPVVLCHNDLLCKNIIY-----NEKQGDVQFI
280 290 300 310 320
330 340 350 360 370 380
pF1KE2 DFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQND
:.:::.::: ..:::::: :. : : . :: .. : ... .:: :.. .
XP_016 DYEYSGYNYLAYDIGNHFNEFA-GVSDVDYSL-------YPDRELQSQWLRAYLEAYK-E
330 340 350 360 370
390 400 410 420 430 440
pF1KE2 FENLSTE--EKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDA
:....:: :: . : ....::.:::. . ..: ..
XP_016 FKGFGTEVTEKEV--EILFIQVNQFALTLQSAFSLTAVKRKSGKAYQS
380 390 400 410 420
450
pF1KE2 YFHQKRKLGV
457 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Tue Nov 8 03:26:38 2016 done: Tue Nov 8 03:26:39 2016
Total Scan time: 11.220 Total Display time: 0.020
Function used was FASTA [36.3.4 Apr, 2011]