Result of FASTA (omim) for
pFN21AE1945
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE1945, 500 aa
1>>>pF1KE1945 500 - 500 aa - 500 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 4.9889+/-0.000383; mu= 20.1778+/- 0.024
mean_var=74.6576+/-15.631, 0's: 0 Z-trim(113.1): 103 B-trim: 179 in 1/56
Lambda= 0.148435
statistics sampled from 22225 (22329) to 22225 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.63), E-opt: 0.2 (0.262), width: 16
Scan time: 8.680
The best scores are: opt bits E(85289)
NP_000952 (OMIM: 145500,601699) prostacyclin synth ( 500) 3345 726.1 5.8e-209
NP_004382 (OMIM: 602172) 7-alpha-hydroxycholest-4- ( 501) 1303 288.8 2.5e-77
NP_000771 (OMIM: 118455) cholesterol 7-alpha-monoo ( 504) 923 207.4 7.9e-53
NP_004811 (OMIM: 270800,603711,613812) 25-hydroxyc ( 506) 829 187.3 9.1e-47
XP_016869491 (OMIM: 270800,603711,613812) PREDICTE ( 528) 782 177.2 1e-43
NP_001311041 (OMIM: 270800,603711,613812) 25-hydro ( 460) 620 142.5 2.5e-33
XP_016866414 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 335) 182 48.6 3.4e-05
XP_011512960 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 371) 182 48.6 3.7e-05
XP_005249228 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 420) 182 48.6 4.1e-05
XP_016866413 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 433) 182 48.7 4.2e-05
XP_016866412 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 435) 182 48.7 4.2e-05
NP_057677 (OMIM: 605994) 24-hydroxycholesterol 7-a ( 469) 182 48.7 4.4e-05
XP_016866410 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 471) 182 48.7 4.4e-05
NP_000777 (OMIM: 601637) lanosterol 14-alpha demet ( 509) 180 48.3 6.3e-05
NP_085125 (OMIM: 611529) cytochrome P450 2S1 precu ( 504) 177 47.6 9.8e-05
NP_001265667 (OMIM: 605994) 24-hydroxycholesterol ( 449) 173 46.7 0.00016
XP_016866411 (OMIM: 605994) PREDICTED: 24-hydroxyc ( 451) 173 46.7 0.00016
NP_001265668 (OMIM: 605994) 24-hydroxycholesterol ( 297) 163 44.4 0.00053
NP_000488 (OMIM: 103900,202010,610613) cytochrome ( 503) 148 41.4 0.0072
>>NP_000952 (OMIM: 145500,601699) prostacyclin synthase (500 aa)
initn: 3345 init1: 3345 opt: 3345 Z-score: 3872.5 bits: 726.1 E(85289): 5.8e-209
Smith-Waterman score: 3345; 100.0% identity (100.0% similar) in 500 aa overlap (1-500:1-500)
10 20 30 40 50 60
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE1 HGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 HGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDE
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE1 KARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 KARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTL
130 140 150 160 170 180
190 200 210 220 230 240
pF1KE1 YGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 YGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKL
190 200 210 220 230 240
250 260 270 280 290 300
pF1KE1 LSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 LSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLL
250 260 270 280 290 300
310 320 330 340 350 360
pF1KE1 KNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITRE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 KNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITRE
310 320 330 340 350 360
370 380 390 400 410 420
pF1KE1 VVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDF
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 VVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDF
370 380 390 400 410 420
430 440 450 460 470 480
pF1KE1 YKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSR
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_000 YKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSR
430 440 450 460 470 480
490 500
pF1KE1 YGFGLMQPEHDVPVRYRIRP
::::::::::::::::::::
NP_000 YGFGLMQPEHDVPVRYRIRP
490 500
>>NP_004382 (OMIM: 602172) 7-alpha-hydroxycholest-4-en-3 (501 aa)
initn: 1292 init1: 907 opt: 1303 Z-score: 1509.2 bits: 288.8 E(85289): 2.5e-77
Smith-Waterman score: 1303; 40.9% identity (69.4% similar) in 506 aa overlap (3-500:4-499)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALL--LLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRM
:. .:: : ... : : . : ::: ::::: :..::::.:. : :. :: ::
NP_004 MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRM
10 20 30 40 50 60
60 70 80 90 100 110
pF1KE1 KEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIF---DVQLPH
. ::::.::. .::.: : ..:: :. ... . . .::: :: :. ..: .:: :
NP_004 RTKHGDVFTVQLGGQYFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDH
70 80 90 100 110 120
120 130 140 150 160 170
pF1KE1 YSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLL---GDATEAGSGWHEMGLLDFSYSF
. ... : :. :.:.: .: :.: : . .: : ::: .:. : : .
NP_004 EMIHSASTKH---LRGDGLKDLNETMLDSLSFVMLTSKGWSLDA-SCWHEDSLFRFCYYI
130 140 150 160 170
180 190 200 210 220 230
pF1KE1 LLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMC
:. ::::.:.: ... :: ......: ::..: :.:... . : . ..
NP_004 LFTAGYLSLFGYT------KDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVG
180 190 200 210 220 230
240 250 260 270 280 290
pF1KE1 SVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPA
.. . :.:: .. .. :.:: ..: :.:.:: :: . ..:::.::: ::.
NP_004 RLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPT
240 250 260 270 280 290
300 310 320 330 340 350
pF1KE1 AFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRL
.:: ::.:::.:::. ::: : ..: .:. ..:. .. .:. :::::::. :.:::
NP_004 SFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRL
300 310 320 330 340 350
360 370 380 390 400 410
pF1KE1 TAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLN
::: . : : : .. :..:.:. .:.:: : :::.:: . ::.:. .: ::::.::::
NP_004 RAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLN
360 370 380 390 400 410
420 430 440 450 460 470
pF1KE1 PDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADV
:.::.: ::.: ::....:.::::.: . : :: .:.. .: :..:...:.::::.. :.
NP_004 PNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDT
420 430 440 450 460 470
480 490 500
pF1KE1 EIPEFDLSRYGFGLMQPEHDVPVRYRIRP
.:. : .:.::: ::: ::: :::..:
NP_004 PLPHVDPQRWGFGTMQPSHDVRFRYRLHPTE
480 490 500
>>NP_000771 (OMIM: 118455) cholesterol 7-alpha-monooxyge (504 aa)
initn: 675 init1: 349 opt: 923 Z-score: 1069.4 bits: 207.4 E(85289): 7.9e-53
Smith-Waterman score: 923; 33.0% identity (64.2% similar) in 509 aa overlap (1-499:6-502)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLT
. :. .. : :.: . ::.: :::::. : ::.:: ::.:: . ::
NP_000 MMTTSLIWGIAIAACCCLWLILGIRRRQT---GEPPLENGLIPYLGCALQFGANPLEFLR
10 20 30 40 50
60 70 80 90 100 110
pF1KE1 RMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPR----TRLDFHAYAIFLMERIFDVQ
..::: .:: . :.:: . .: :: :. . . .. : . : . .: .: .
NP_000 ANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPM
60 70 80 90 100 110
120 130 140 150 160
pF1KE1 LPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAG--SGWHEMGLLDFSY
. . . . . .: :: . :..:::.:. ::. .. .. . ..: :. .: :
NP_000 DGNTTENINDTFIK-TLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCY
120 130 140 150 160 170
170 180 190 200 210 220
pF1KE1 SFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDH
...:::::..: . : : ...: ..: . . .:.:.:...: :. : : .
NP_000 RVMFEAGYLTIFGRD-LTR-RDTQ---KAHILNNLDNFKQFDKVFPALVAG-LPIHMFRT
180 190 200 210 220 230
230 240 250 260 270 280
pF1KE1 MCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQ-ARALVLQLWATQGNM
... .: . : : .: :. . .. . ... .... :.. .. :::.:.:
NP_000 AHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANT
240 250 260 270 280 290
290 300 310 320 330 340
pF1KE1 GPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVS---QTTTLPQKVLDSTPVLDSVL
::.:: :. ...::::. :. :.. : .: : :: . : : :.. :::::..
NP_000 IPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSII
300 310 320 330 340 350
350 360 370 380 390 400
pF1KE1 SESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFK
.:::::..: . : . :... . :: .:.:. : . :.: : . ::::: :: .::
NP_000 KESLRLSSASLNIRTAKEDFTLHLEDGS-YNIRKDDIIALYPQLM-HLDPEIYPDPLTFK
360 370 380 390 400
410 420 430 440 450 460
pF1KE1 YNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLE
:.:.:. .:. : :: .: .:: : ::.:.: . : :: .:.. ::::..:.: ...::
NP_000 YDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELE
410 420 430 440 450 460
470 480 490 500
pF1KE1 LINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
::..... : .: :: :.:.. : .:. .:...
NP_000 LIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
470 480 490 500
>>NP_004811 (OMIM: 270800,603711,613812) 25-hydroxychole (506 aa)
initn: 695 init1: 277 opt: 829 Z-score: 960.6 bits: 187.3 E(85289): 9.1e-47
Smith-Waterman score: 882; 34.1% identity (63.9% similar) in 499 aa overlap (7-499:22-505)
10 20 30 40
pF1KE1 MAWAALLGLLAALLLLLL-LSRRRTRRPGEPPLDLGSIPWLGYAL
:.: :::::: : : ::::::::::: : .:.:: .:
NP_004 MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVL
10 20 30 40 50 60
50 60 70 80 90 100
pF1KE1 DFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLM
.. :: :. ....::: ::.:.::.:.: .::: .:. :. . . .:.:.... :.
NP_004 NLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVI-KNHKQLSFRVFSNKLL
70 80 90 100 110
110 120 130 140 150 160
pF1KE1 ERIFDV-QLPHYSPSDEKARMKLTLLH-RELQALTEAMYTNLHAVLLGDATEAGSGWHEM
:. :.. :: . ... .. .:. . :. : :.:. ::. :. . .. : :
NP_004 EKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTS-WDTA
120 130 140 150 160 170
170 180 190 200 210 220
pF1KE1 GLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSL
: : :.... . :.:: . ... .. : : ..: . :. ...
NP_004 ELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELR-------DDFLKFDDKFAYLV-SNI
180 190 200 210 220 230
230 240 250 260 270 280
pF1KE1 SVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ-L
. .. :.. .. : .: .::. :. ..: ::.. : :... : : :
NP_004 PIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFL
240 250 260 270 280 290
290 300 310 320 330
pF1KE1 WATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQ--TTTLPQKVLDSTP
::. .: :. :: . .::..:::.:::: :.. .: .. : .. : .. :::
NP_004 WASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLI
300 310 320 330 340 350
340 350 360 370 380 390
pF1KE1 VLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYT
:.: . :.:::.. : : ::.. : .. .:.:: . .:: . . ::::.
NP_004 CLESSIFEALRLSSYSTTIRFVEEDLTLSSETG-DYCVRKGDLVAIFPPVL-HGDPEIFE
360 370 380 390 400
400 410 420 430 440 450
pF1KE1 DPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVL
:: :.:.::.. ::..: :.: ::.:: : ::.:.: ..: :: .:. :::.. ..:
NP_004 APEEFRYDRFIE-DGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILL
410 420 430 440 450 460
460 470 480 490 500
pF1KE1 VHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
...:::.:. : : .. :: ::.. :. :: ::...
NP_004 TYFDLEIID-DKPIG-LNYSRLLFGIQYPDSDVLFRYKVKS
470 480 490 500
>>XP_016869491 (OMIM: 270800,603711,613812) PREDICTED: 2 (528 aa)
initn: 723 init1: 240 opt: 782 Z-score: 905.9 bits: 177.2 E(85289): 1e-43
Smith-Waterman score: 835; 33.1% identity (63.5% similar) in 480 aa overlap (25-499:63-527)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFL
:::::::: : .:.:: .:.. :: :.
XP_016 EDIQLMLTDCNPLRQDLDIYTLQLPEKTCLRRPGEPPLIKGWLPYLGVVLNLRKDPLRFM
40 50 60 70 80 90
60 70 80 90 100 110
pF1KE1 TRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDV-QLP
....::: ::.:.::.:.: .::: .:. :. . . .:.:.... :.:. :.. ::
XP_016 KTLQKQHGDTFTVLLGGKYITFILDPFQYQLVI-KNHKQLSFRVFSNKLLEKAFSISQLQ
100 110 120 130 140 150
120 130 140 150 160 170
pF1KE1 HYSPSDEKARMKLTLLH-RELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFL
. ... .. .:. . :. : :.:. ::. :. . .. : : : : :..
XP_016 KNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTS-WDTAELYPFCSSII
160 170 180 190 200 210
180 190 200 210 220 230
pF1KE1 LRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCS
.. . :.:: . ... .. : : : : : . :. ... . .. :
XP_016 FEITFTTIYGKVIVCDNNKFISELR----DDFLKF---DDKFAYLV-SNIPIELLGNVKS
220 230 240 250 260
240 250 260 270 280 290
pF1KE1 VKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ-LWATQGNMGPA
.. .. : .: .::. :. ..: ::.. : :... : : :::. .: :.
XP_016 IREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPT
270 280 290 300 310 320
300 310 320 330 340
pF1KE1 AFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQ--TTTLPQKVLDSTPVLDSVLSESL
:: . .::..:::.:::: :.. .: .. : .. : .. ::: :.: . :.:
XP_016 MFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEAL
330 340 350 360 370 380
350 360 370 380 390 400
pF1KE1 RLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRF
::.. : : ::.. : .. .:.:: . .:: . . ::::. :: :.:.::
XP_016 RLSSYSTTIRFVEEDLTLSSETG-DYCVRKGDLVAIFPPVL-HGDPEIFEAPEEFRYDRF
390 400 410 420 430 440
410 420 430 440 450 460
pF1KE1 LNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINA
.. ::..: :.: ::.:: : ::.:.: ..: :: .:. :::.. ..:...:::.:.
XP_016 IE-DGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIID-
450 460 470 480 490
470 480 490 500
pF1KE1 DVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
: : .. :: ::.. :. :: ::...
XP_016 DKPIG-LNYSRLLFGIQYPDSDVLFRYKVKS
500 510 520
>>NP_001311041 (OMIM: 270800,603711,613812) 25-hydroxych (460 aa)
initn: 483 init1: 277 opt: 620 Z-score: 719.2 bits: 142.5 E(85289): 2.5e-33
Smith-Waterman score: 620; 32.1% identity (61.4% similar) in 402 aa overlap (7-402:22-411)
10 20 30 40
pF1KE1 MAWAALLGLLAALLLLLL-LSRRRTRRPGEPPLDLGSIPWLGYAL
:.: :::::: : : ::::::::::: : .:.:: .:
NP_001 MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVL
10 20 30 40 50 60
50 60 70 80 90 100
pF1KE1 DFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLM
.. :: :. ....::: ::.:.::.:.: .::: .:. :. . . .:.:.... :.
NP_001 NLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVI-KNHKQLSFRVFSNKLL
70 80 90 100 110
110 120 130 140 150 160
pF1KE1 ERIFDV-QLPHYSPSDEKARMKLTLLH-RELQALTEAMYTNLHAVLLGDATEAGSGWHEM
:. :.. :: . ... .. .:. . :. : :.:. ::. :. . .. : :
NP_001 EKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTS-WDTA
120 130 140 150 160 170
170 180 190 200 210 220
pF1KE1 GLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSL
: : :.... . :.:: . ... .. : : ..: . :. ...
NP_001 ELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELR-------DDFLKFDDKFAYLV-SNI
180 190 200 210 220 230
230 240 250 260 270 280
pF1KE1 SVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ-L
. .. :.. .. : .: .::. :. ..: ::.. : :... : : :
NP_001 PIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFL
240 250 260 270 280 290
290 300 310 320 330
pF1KE1 WATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQ--TTTLPQKVLDSTP
::. .: :. :: . .::..:::.:::: :.. .: .. : .. : .. :::
NP_001 WASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLI
300 310 320 330 340 350
340 350 360 370 380 390
pF1KE1 VLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYT
:.: . :.:::.. : : ::.. : .. .:.:: . .:: . . ::::.
NP_001 CLESSIFEALRLSSYSTTIRFVEEDLTLSSETG-DYCVRKGDLVAIFPPVL-HGDPEIFE
360 370 380 390 400
400 410 420 430 440 450
pF1KE1 DPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVL
::
NP_001 APEQTVLTGETRGMEITELSNLYFCTAYSREKWRRERRKIHTHILVYLIILQ
410 420 430 440 450 460
>>XP_016866414 (OMIM: 605994) PREDICTED: 24-hydroxychole (335 aa)
initn: 207 init1: 132 opt: 182 Z-score: 214.2 bits: 48.6 E(85289): 3.4e-05
Smith-Waterman score: 211; 24.2% identity (50.0% similar) in 360 aa overlap (9-362:11-313)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMK
.:. : :.:::.:. ::: : : :::.: ...::: :. . .
XP_016 MELISPTVIIILGCLALFLLLQRKNLRRP---PCIKGWIPWIGVGFEFGKAPLEFIEKAR
10 20 30 40 50
60 70 80 90 100 110
pF1KE1 EKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPS
:.: :::... : .: . . .. . :. ..::. . .. : .. .
XP_016 IKYGPIFTVFAMGNRMTFVTEEEGIN--VFLKSKKVDFELAVQNIVYRTASIPKNVFLAL
60 70 80 90 100 110
120 130 140 150 160 170
pF1KE1 DEK------ARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFL
:: ..: . ::. ::: .. .:. . : : : .:.. :
XP_016 HEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENL----------GTH--GTMDLNN--L
120 130 140 150 160
180 190 200 210 220 230
pF1KE1 LRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCS
.: .: :: . . ..: . .. :: . : : :.:
XP_016 VR--HL-LYPVTVNMLFNKSLFSTNKKKIKEFHQYFQ--------------VYDEDF--E
170 180 190 200
240 250 260 270 280 290
pF1KE1 VKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAA
:.: : : : .:: ...:.: : .. . .. : ...: .:
XP_016 YGSQL-----PECLLRNWSKSK---KWFLELFEKNIPDIKACKS------AKDNSM--VA
210 220 230 240
300 310 320 330 340 350
pF1KE1 FWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLT
:: : ..:..:. :. . :.. .: . ... . :.. .. . :..::
XP_016 FWTLAYVLSHPDIHKAIMEGISSVFGKAGKDKIKVS---EDDLENLLLIKWCVLETIRLK
250 260 270 280 290 300
360 370 380 390 400 410
pF1KE1 AAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNP
: :::.::
XP_016 APGVITRKVVKPVEILVVCSVRGSDVYYFNTL
310 320 330
>>XP_011512960 (OMIM: 605994) PREDICTED: 24-hydroxychole (371 aa)
initn: 244 init1: 132 opt: 182 Z-score: 213.6 bits: 48.6 E(85289): 3.7e-05
Smith-Waterman score: 270; 24.7% identity (51.1% similar) in 372 aa overlap (9-362:11-349)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMK
.:. : :.:::.:. ::: : : :::.: ...::: :. . .
XP_011 MELISPTVIIILGCLALFLLLQRKNLRRP---PCIKGWIPWIGVGFEFGKAPLEFIEKAR
10 20 30 40 50
60 70 80 90 100 110
pF1KE1 EKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPS
:.: :::... : .: . . .. . :. ..::. . .. : .. .
XP_011 IKYGPIFTVFAMGNRMTFVTEEEGIN--VFLKSKKVDFELAVQNIVYRTASIPKNVFLAL
60 70 80 90 100 110
120 130 140 150 160 170
pF1KE1 DEK------ARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFL
:: ..: . ::. ::: .. .:. . : : : .:.. :
XP_011 HEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENL----------GTH--GTMDLNN--L
120 130 140 150 160
180 190 200 210 220 230
pF1KE1 LRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQL-DRLLPKLARGSLSVGDKDHMC
.: .: :: . . ..: . .. :: . :. :. .. :.. :
XP_011 VR--HL-LYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDE--------DFEYGSQLPEC
170 180 190 200 210
240 250 260 270 280
pF1KE1 SV----KSRLW------KLLSPARLARRAH-RSKWLESYLLHLEEMGVSEEMQARALVLQ
. ::. : : . . . :. : : . : . : .:.: . .:
XP_011 LLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLL
220 230 240 250 260 270
290 300 310 320 330 340
pF1KE1 LWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPV
:::. .: :.::: : ..:..:. :. . :.. .: . ... . :.. .
XP_011 LWASLSNAVPVAFWTLAYVLSHPDIHKAIMEGISSVFGKAGKDKIKVS---EDDLENLLL
280 290 300 310 320
350 360 370 380 390 400
pF1KE1 LDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTD
. . :..:: : :::.::
XP_011 IKWCVLETIRLKAPGVITRKVVKPVEILVVCSVRGSDVYYFNTL
330 340 350 360 370
>>XP_005249228 (OMIM: 605994) PREDICTED: 24-hydroxychole (420 aa)
initn: 347 init1: 132 opt: 182 Z-score: 212.9 bits: 48.6 E(85289): 4.1e-05
Smith-Waterman score: 341; 25.1% identity (52.0% similar) in 454 aa overlap (9-444:11-417)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMK
.:. : :.:::.:. ::: : : :::.: ...::: :. . .
XP_005 MELISPTVIIILGCLALFLLLQRKNLRRP---PCIKGWIPWIGVGFEFGKAPLEFIEKAR
10 20 30 40 50
60 70 80 90 100 110
pF1KE1 EKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPS
:.: :::... : .: . . .. . :. ..::. . .. : .. .
XP_005 IKYGPIFTVFAMGNRMTFVTEEEGIN--VFLKSKKVDFELAVQNIVYRTASIPKNVFLAL
60 70 80 90 100 110
120 130 140 150 160 170
pF1KE1 DEK------ARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFL
:: ..: . ::. ::: .. .:. . : : : .:.. :
XP_005 HEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENL----------GTH--GTMDLNN--L
120 130 140 150 160
180 190 200 210 220 230
pF1KE1 LRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQL-DRLLPKLARGSLSVGDKDHMC
.: .: :: . . ..: . .. :: . :. :. .. :.. :
XP_005 VR--HL-LYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDE--------DFEYGSQLPEC
170 180 190 200 210
240 250 260 270 280
pF1KE1 SV----KSRLW------KLLSPARLARRAH-RSKWLESYLLHLEEMGVSEEMQARALVLQ
. ::. : : . . . :. : : . : . : .:.: . .:
XP_005 LLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLL
220 230 240 250 260 270
290 300 310 320 330 340
pF1KE1 LWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPV
:::. .: :.::: : ..:..:. :. . :.. .: . ... . :.. .
XP_005 LWASLSNAVPVAFWTLAYVLSHPDIHKAIMEGISSVFGKAGKDKIKVS---EDDLENLLL
280 290 300 310 320
350 360 370 380 390 400
pF1KE1 LDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTD
. . :..:: : :::.:: . . .. . :: :.: :: .:.:. . .
XP_005 IKWCVLETIRLKAPGVITRKVVKPVEI-----LNYIIPSGDLLMLSPFWL-HRNPKYFPE
330 340 350 360 370 380
410 420 430 440 450 460
pF1KE1 PEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLV
::.:: .:. . . ::..: : . : .:.:. .: .:
XP_005 PELFKPERWKKAN-LEKHSF------LDCF-MAFGSGKFQCPARKGF
390 400 410 420
470 480 490 500
pF1KE1 HLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
>>XP_016866413 (OMIM: 605994) PREDICTED: 24-hydroxychole (433 aa)
initn: 244 init1: 132 opt: 182 Z-score: 212.7 bits: 48.7 E(85289): 4.2e-05
Smith-Waterman score: 333; 24.1% identity (51.3% similar) in 497 aa overlap (9-499:11-432)
10 20 30 40 50
pF1KE1 MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMK
.:. : :.:::.:. ::: : : :::.: ...::: :. . .
XP_016 MELISPTVIIILGCLALFLLLQRKNLRRP---PCIKGWIPWIGVGFEFGKAPLEFIEKAR
10 20 30 40 50
60 70 80 90 100 110
pF1KE1 EKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPS
:.: :::... : .: . . .. . :. ..::. . .. : .. .
XP_016 IKYGPIFTVFAMGNRMTFVTEEEGIN--VFLKSKKVDFELAVQNIVYRTASIPKNVFLAL
60 70 80 90 100 110
120 130 140 150 160 170
pF1KE1 DEK------ARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFL
:: ..: . ::. ::: .. .:. . : : : .:.. :
XP_016 HEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENL----------GTH--GTMDLNN--L
120 130 140 150 160
180 190 200 210 220 230
pF1KE1 LRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCS
.: :: . . ..: . .. :: . : : :.:
XP_016 VRH---LLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQ--------------VYDEDF--E
170 180 190 200
240 250 260 270 280 290
pF1KE1 VKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAA
:.: : : : .:: ...:.: : .. . .. : ...: .:
XP_016 YGSQL-----PECLLRNWSKSK---KWFLELFEKNIPDIKACKS------AKDNSM--VA
210 220 230 240
300 310 320 330 340 350
pF1KE1 FWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLT
:: : ..:..:. :. . :.. .: . . . . :.. .. . :..::
XP_016 FWTLAYVLSHPDIHKAIMEGISSVFGKAGK---DKIKVSEDDLENLLLIKWCVLETIRLK
250 260 270 280 290 300
360 370 380 390 400 410
pF1KE1 AAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNP
: :::.:: . . .. . :: :.: :: .:.:. . .::.:: .:. .
XP_016 APGVITRKVVKPVEI-----LNYIIPSGDLLMLSPFWL-HRNPKYFPEPELFKPERWKKA
310 320 330 340 350
420 430 440 450 460 470
pF1KE1 DGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVE
. ::..: : . : .:.:. .: .: .:. ... ..:.: . : :..
XP_016 N-LEKHSF------LDCF-MAFGSGKFQCPARWFALLEVQMCIILILYKYDCSLLD---P
360 370 380 390 400
480 490 500
pF1KE1 IPEFDLSRYGFGLMQPEHDVPVRYRIRP
.:. . . :. ::: . ..:. :
XP_016 LPKQSYLHL-VGVPQPEGQCRIEYKQRI
410 420 430
500 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Mon Nov 7 15:50:05 2016 done: Mon Nov 7 15:50:06 2016
Total Scan time: 8.680 Total Display time: 0.090
Function used was FASTA [36.3.4 Apr, 2011]