Restriction map for
pFN21AE9695
(Linear) (Six-base) MAPSORT of: KIEE9695.seq Check: 8310 from: 1 to: 1965
KIEE9695.orf
With 182 enzymes: SgfI *
December 5, 2014 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1344 1952 1965
Size: 1344 608 13
Fragments arranged by size:
1344 608 13
AatII G_ACGT'C
Cuts at: 0 1570 1965
Size: 1570 395
AccI GT'mk_AC
Cuts at: 0 767 1965
Size: 767 1198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 1765 1965
Size: 1414 351 200
Fragments arranged by size:
1414 351 200
AflII C'TTAA_G
Cuts at: 0 1867 1965
Size: 1867 98
AflIII A'CryG_T
Cuts at: 0 65 907 1965
Size: 65 842 1058
Fragments arranged by size:
1058 842 65
AleI CACnn'nnGTG
Cuts at: 0 10 1965
Size: 10 1955
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 1965
Size: 1264 32 669
Fragments arranged by size:
1264 669 32
AlwNI CAG_nnn'CTG
Cuts at: 0 746 975 1965
Size: 746 229 990
Fragments arranged by size:
990 746 229
ApaI G_GGCC'C
Cuts at: 0 698 1965
Size: 698 1267
ApaLI G'TGCA_C
Cuts at: 0 1665 1965
Size: 1665 300
ApoI r'AATT_y
Cuts at: 0 287 1965
Size: 287 1678
AvaI C'yCGr_G
Cuts at: 0 220 935 1965
Size: 220 715 1030
Fragments arranged by size:
1030 715 220
BanI G'GyrC_C
Cuts at: 0 1287 1965
Size: 1287 678
BanII G_rGCy'C
Cuts at: 0 230 256 698 1205 1385 1965
Size: 230 26 442 507 180 580
Fragments arranged by size:
580 507 442 230 180 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 411 1965
Size: 411 1554
BglI GCCn_nnn'nGGC
Cuts at: 0 179 832 1389 1910 1919 1965
Size: 179 653 557 521 9 46
Fragments arranged by size:
653 557 521 179 46 9
BglII A'GATC_T
Cuts at: 0 1151 1965
Size: 1151 814
BlpI GC'TnA_GC
Cuts at: 0 1349 1965
Size: 1349 616
Bme1580I G_kGCm'C
Cuts at: 0 437 698 1669 1965
Size: 437 261 971 296
Fragments arranged by size:
971 437 296 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 187 822 1312 1965
Size: 187 635 490 653
Fragments arranged by size:
653 635 490 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1318 1965
Size: 673 645 647
Fragments arranged by size:
673 647 645
Bpu10I CC'TnA_GC
Cuts at: 0 782 1906 1965
Size: 782 1124 59
Fragments arranged by size:
1124 782 59
BsaAI yAC'GTr
Cuts at: 0 894 1965
Size: 894 1071
BsaHI Gr'CG_yC
Cuts at: 0 487 1183 1567 1965
Size: 487 696 384 398
Fragments arranged by size:
696 487 398 384
BsaWI w'CCGG_w
Cuts at: 0 458 1965
Size: 458 1507
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 871 901 1965
Size: 871 30 1064
Fragments arranged by size:
1064 871 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 876 1843 1965
Size: 238 638 967 122
Fragments arranged by size:
967 638 238 122
BseYI C'CCAG_C
Cuts at: 0 81 773 985 1018 1081 1258 1430 1965
Size: 81 692 212 33 63 177 172 535
Fragments arranged by size:
692 535 212 177 172 81 63 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1196 1297 1322 1719 1924 1965
Size: 967 229 101 25 397 205 41
Fragments arranged by size:
967 397 229 205 101 41 25
BsiEI CG_ry'CG
Cuts at: 0 494 1914 1965
Size: 494 1420 51
Fragments arranged by size:
1420 494 51
BsiHKAI G_wGCw'C
Cuts at: 0 230 1505 1669 1965
Size: 230 1275 164 296
Fragments arranged by size:
1275 296 230 164
BsiWI C'GTAC_G
Cuts at: 0 344 1965
Size: 344 1621
BsmBI CGTCTCn'nnnn_
Cuts at: 0 888 1965
Size: 888 1077
Bsp1286I G_dGCh'C
Cuts at: 0 230 256 437 698 1205 1385 1505 1669
Size: 230 26 181 261 507 180 120 164
Cuts at: 1669 1965
Size: 296
Fragments arranged by size:
507 296 261 230 181 180 164 120
26
BspHI T'CATG_A
Cuts at: 0 167 1965
Size: 167 1798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1036 1344 1952 1965
Size: 1036 308 608 13
Fragments arranged by size:
1036 608 308 13
BsrBI CCG'CTC
Cuts at: 0 786 1014 1910 1965
Size: 786 228 896 55
Fragments arranged by size:
896 786 228 55
BsrDI GCAATG_nn'
Cuts at: 0 849 1965
Size: 849 1116
BsrFI r'CCGG_y
Cuts at: 0 238 532 956 1847 1965
Size: 238 294 424 891 118
Fragments arranged by size:
891 424 294 238 118
BsrGI T'GTAC_A
Cuts at: 0 737 1965
Size: 737 1228
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1028 1965
Size: 1028 937
BstEII G'GTnAC_C
Cuts at: 0 15 1965
Size: 15 1950
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1497 1965
Size: 12 1485 468
Fragments arranged by size:
1485 468 12
BstYI r'GATC_y
Cuts at: 0 522 900 1124 1151 1424 1469 1965
Size: 522 378 224 27 273 45 496
Fragments arranged by size:
522 496 378 273 224 45 27
BtgI C'CryG_G
Cuts at: 0 1268 1550 1917 1965
Size: 1268 282 367 48
Fragments arranged by size:
1268 367 282 48
BtsI GCAGTG_nn'
Cuts at: 0 527 757 973 1965
Size: 527 230 216 992
Fragments arranged by size:
992 527 230 216
DraIII CAC_nnn'GTG
Cuts at: 0 510 763 1493 1965
Size: 510 253 730 472
Fragments arranged by size:
730 510 472 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 1965
Size: 130 1835
EaeI y'GGCC_r
Cuts at: 0 2 236 491 824 833 1586 1911 1952
Size: 2 234 255 333 9 753 325 41
Cuts at: 1952 1965
Size: 13
Fragments arranged by size:
753 333 325 255 234 41 13 9
2
EagI C'GGCC_G
Cuts at: 0 491 1911 1965
Size: 491 1420 54
Fragments arranged by size:
1420 491 54
EarI CTCTTCn'nnn_
Cuts at: 0 718 1965
Size: 718 1247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 1965
Size: 196 1769
EcoICRI GAG'CTC
Cuts at: 0 228 1965
Size: 228 1737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1318 1414 1765 1965
Size: 673 645 96 351 200
Fragments arranged by size:
673 645 351 200 96
EcoO109I rG'GnC_Cy
Cuts at: 0 270 568 648 939 1302 1965
Size: 270 298 80 291 363 663
Fragments arranged by size:
663 363 298 291 270 80
EcoRV GAT'ATC
Cuts at: 0 1818 1965
Size: 1818 147
HaeII r_GCGC'y
Cuts at: 0 992 1074 1965
Size: 992 82 891
Fragments arranged by size:
992 891 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1447 1479 1965
Size: 1096 32 319 32 486
Fragments arranged by size:
1096 486 319 32 32
HincII GTy'rAC
Cuts at: 0 135 576 810 1632 1965
Size: 135 441 234 822 333
Fragments arranged by size:
822 441 333 234 135
HindIII A'AGCT_T
Cuts at: 0 1693 1965
Size: 1693 272
MluI A'CGCG_T
Cuts at: 0 907 1965
Size: 907 1058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1427 1965
Size: 1159 268 538
Fragments arranged by size:
1159 538 268
MscI TGG'CCA
Cuts at: 0 4 835 1588 1954 1965
Size: 4 831 753 366 11
Fragments arranged by size:
831 753 366 11 4
MslI CAynn'nnrTG
Cuts at: 0 10 301 1273 1495 1783 1965
Size: 10 291 972 222 288 182
Fragments arranged by size:
972 291 288 222 182 10
MspA1I CmG'CkG
Cuts at: 0 743 1582 1761 1880 1965
Size: 743 839 179 119 85
Fragments arranged by size:
839 743 179 119 85
NaeI GCC'GGC
Cuts at: 0 240 534 1849 1965
Size: 240 294 1315 116
Fragments arranged by size:
1315 294 240 116
NcoI C'CATG_G
Cuts at: 0 1268 1550 1917 1965
Size: 1268 282 367 48
Fragments arranged by size:
1268 367 282 48
NgoMIV G'CCGG_C
Cuts at: 0 238 532 1847 1965
Size: 238 294 1315 118
Fragments arranged by size:
1315 294 238 118
NotI GC'GGCC_GC
Cuts at: 0 1911 1965
Size: 1911 54
NspI r_CATG'y
Cuts at: 0 58 1788 1965
Size: 58 1730 177
Fragments arranged by size:
1730 177 58
PpuMI rG'GwC_Cy
Cuts at: 0 270 648 939 1302 1965
Size: 270 378 291 363 663
Fragments arranged by size:
663 378 363 291 270
PshAI GACnn'nnGTC
Cuts at: 0 910 1965
Size: 910 1055
PspOMI G'GGCC_C
Cuts at: 0 694 1965
Size: 694 1271
PstI C_TGCA'G
Cuts at: 0 328 1033 1703 1760 1965
Size: 328 705 670 57 205
Fragments arranged by size:
705 670 328 205 57
SacI G_AGCT'C
Cuts at: 0 230 1965
Size: 230 1735
SanDI GG'GwC_CC
Cuts at: 0 939 1965
Size: 939 1026
SfcI C'TryA_G
Cuts at: 0 110 324 1029 1699 1756 1965
Size: 110 214 705 670 57 209
Fragments arranged by size:
705 670 214 209 110 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 832 1910 1965
Size: 832 1078 55
Fragments arranged by size:
1078 832 55
SmaI CCC'GGG
Cuts at: 0 937 1965
Size: 937 1028
SmlI C'TyrA_G
Cuts at: 0 1867 1965
Size: 1867 98
SphI G_CATG'C
Cuts at: 0 58 1965
Size: 58 1907
StuI AGG'CCT
Cuts at: 0 1399 1904 1965
Size: 1399 505 61
Fragments arranged by size:
1399 505 61
StyI C'CwwG_G
Cuts at: 0 1268 1343 1550 1917 1965
Size: 1268 75 207 367 48
Fragments arranged by size:
1268 367 207 75 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1356 1798 1965
Size: 1356 442 167
Fragments arranged by size:
1356 442 167
TatI w'GTAC_w
Cuts at: 0 737 1965
Size: 737 1228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 1965
Size: 399 32 1534
Fragments arranged by size:
1534 399 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1266 1965
Size: 1266 699
XmaI C'CCGG_G
Cuts at: 0 935 1965
Size: 935 1030
ZraI GAC'GTC
Cuts at: 0 1568 1965
Size: 1568 397
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV HaeII Hin4I HincII HindIII MluI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NotI NspI PpuMI
PshAI PspOMI PstI SacI SanDI SfcI SfiI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AlfI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsmI BspEI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EcoNI EcoRI FalI FseI FspI
FspAI HpaI KasI KpnI MfeI NarI NdeI NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI