Restriction map for
pFN21AE9694
(Linear) (Six-base) MAPSORT of: KIEE9694.seq Check: 2830 from: 1 to: 948
KIEE9694.orf
With 182 enzymes: SgfI *
December 5, 2014 13:31 ..
AccI GT'mk_AC
Cuts at: 0 55 948
Size: 55 893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 796 948
Size: 619 177 152
Fragments arranged by size:
619 177 152
AflII C'TTAA_G
Cuts at: 0 919 948
Size: 919 29
AlwNI CAG_nnn'CTG
Cuts at: 0 148 948
Size: 148 800
AvaI C'yCGr_G
Cuts at: 0 314 948
Size: 314 634
BanII G_rGCy'C
Cuts at: 0 204 524 948
Size: 204 320 424
Fragments arranged by size:
424 320 204
BclI T'GATC_A
Cuts at: 0 729 948
Size: 729 219
BlpI GC'TnA_GC
Cuts at: 0 283 948
Size: 283 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 859 948
Size: 649 210 89
Fragments arranged by size:
649 210 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 948
Size: 508 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 691 948
Size: 691 257
BsaWI w'CCGG_w
Cuts at: 0 92 948
Size: 92 856
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 264 784 826 948
Size: 214 50 520 42 122
Fragments arranged by size:
520 214 122 50 42
BsiHKAI G_wGCw'C
Cuts at: 0 524 948
Size: 524 424
BsmBI CGTCTCn'nnnn_
Cuts at: 0 417 948
Size: 417 531
Bsp1286I G_dGCh'C
Cuts at: 0 204 524 948
Size: 204 320 424
Fragments arranged by size:
424 320 204
BsrBI CCG'CTC
Cuts at: 0 287 948
Size: 287 661
BsrFI r'CCGG_y
Cuts at: 0 130 194 297 948
Size: 130 64 103 651
Fragments arranged by size:
651 130 103 64
BtsI GCAGTG_nn'
Cuts at: 0 455 948
Size: 455 493
DraI TTT'AAA
Cuts at: 0 164 948
Size: 164 784
EaeI y'GGCC_r
Cuts at: 0 291 948
Size: 291 657
EarI CTCTTCn'nnn_
Cuts at: 0 512 948
Size: 512 436
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 71 948
Size: 71 877
EcoICRI GAG'CTC
Cuts at: 0 522 948
Size: 522 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 649 796 859 948
Size: 619 30 147 63 89
Fragments arranged by size:
619 147 89 63 30
EcoO109I rG'GnC_Cy
Cuts at: 0 603 672 948
Size: 603 69 276
Fragments arranged by size:
603 276 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 351 948
Size: 351 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 570 602 948
Size: 570 32 346
Fragments arranged by size:
570 346 32
HincII GTy'rAC
Cuts at: 0 555 578 948
Size: 555 23 370
Fragments arranged by size:
555 370 23
HindIII A'AGCT_T
Cuts at: 0 778 948
Size: 778 170
MspA1I CmG'CkG
Cuts at: 0 540 948
Size: 540 408
NaeI GCC'GGC
Cuts at: 0 299 948
Size: 299 649
NgoMIV G'CCGG_C
Cuts at: 0 297 948
Size: 297 651
PpuMI rG'GwC_Cy
Cuts at: 0 603 948
Size: 603 345
PstI C_TGCA'G
Cuts at: 0 153 269 948
Size: 153 116 679
Fragments arranged by size:
679 153 116
PvuII CAG'CTG
Cuts at: 0 540 948
Size: 540 408
SacI G_AGCT'C
Cuts at: 0 524 948
Size: 524 424
SapI GCTCTTCn'nnn_
Cuts at: 0 512 948
Size: 512 436
SfcI C'TryA_G
Cuts at: 0 56 149 265 948
Size: 56 93 116 683
Fragments arranged by size:
683 116 93 56
SmlI C'TyrA_G
Cuts at: 0 523 919 948
Size: 523 396 29
Fragments arranged by size:
523 396 29
StuI AGG'CCT
Cuts at: 0 823 948
Size: 823 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 89 948
Size: 89 859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 948
Size: 804 144
Enzymes that do cut:
AccI AcuI AflII AlwNI AvaI BanII BclI BlpI
BpmI BpuEI BsaI BsaWI BseRI BsiHKAI BsmBI Bsp1286I
BsrBI BsrFI BtsI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI Hin4I HincII HindIII MspA1I NaeI
NgoMIV PpuMI PstI PvuII SacI SapI SfcI SmlI
StuI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI