Restriction map for
pFN21AE9692
(Linear) (Six-base) MAPSORT of: KIEE9692.seq Check: 1572 from: 1 to: 1911
KIEE9692.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1110 1553 1911
Size: 1110 443 358
Fragments arranged by size:
1110 443 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1911
Size: 746 1165
AhdI GACnn_n'nnGTC
Cuts at: 0 633 1911
Size: 633 1278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 1911
Size: 1106 805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1586 1618 1911
Size: 1586 32 293
Fragments arranged by size:
1586 293 32
AlwNI CAG_nnn'CTG
Cuts at: 0 787 862 1360 1911
Size: 787 75 498 551
Fragments arranged by size:
787 551 498 75
ApaI G_GGCC'C
Cuts at: 0 771 806 880 1076 1243 1696 1711 1911
Size: 771 35 74 196 167 453 15 200
Fragments arranged by size:
771 453 200 196 167 74 35 15
AvaI C'yCGr_G
Cuts at: 0 901 1000 1408 1486 1883 1911
Size: 901 99 408 78 397 28
Fragments arranged by size:
901 408 397 99 78 28
BanI G'GyrC_C
Cuts at: 0 546 890 1263 1567 1777 1911
Size: 546 344 373 304 210 134
Fragments arranged by size:
546 373 344 304 210 134
BanII G_rGCy'C
Cuts at: 0 535 753 771 806 880 922 1059 1076
Size: 535 218 18 35 74 42 137 17
Cuts at: 1076 1243 1487 1494 1696 1711 1911
Size: 167 244 7 202 15 200
Fragments arranged by size:
535 244 218 202 200 167 137 74
42 35 18 17 15 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 1828 1911
Size: 337 1491 83
Fragments arranged by size:
1491 337 83
BclI T'GATC_A
Cuts at: 0 1769 1911
Size: 1769 142
BfrBI ATG'CAT
Cuts at: 0 1312 1911
Size: 1312 599
BglI GCCn_nnn'nGGC
Cuts at: 0 801 1080 1255 1764 1911
Size: 801 279 175 509 147
Fragments arranged by size:
801 509 279 175 147
BlpI GC'TnA_GC
Cuts at: 0 1493 1911
Size: 1493 418
Bme1580I G_kGCm'C
Cuts at: 0 510 551 771 806 880 1076 1243 1570
Size: 510 41 220 35 74 196 167 327
Cuts at: 1570 1696 1711 1780 1911
Size: 126 15 69 131
Fragments arranged by size:
510 327 220 196 167 131 126 74
69 41 35 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 883 1181 1911
Size: 306 577 298 730
Fragments arranged by size:
730 577 306 298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 1911
Size: 55 1856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 448 566 818 851 1081 1316 1462
Size: 186 262 118 252 33 230 235 146
Cuts at: 1462 1911
Size: 449
Fragments arranged by size:
449 262 252 235 230 186 146 118
33
Bpu10I CC'TnA_GC
Cuts at: 0 515 1024 1661 1911
Size: 515 509 637 250
Fragments arranged by size:
637 515 509 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1911
Size: 1349 562
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 1397 1532 1724 1911
Size: 439 958 135 192 187
Fragments arranged by size:
958 439 192 187 135
BsaHI Gr'CG_yC
Cuts at: 0 1637 1911
Size: 1637 274
BsaWI w'CCGG_w
Cuts at: 0 1303 1817 1911
Size: 1303 514 94
Fragments arranged by size:
1303 514 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 809 839 1721 1751 1911
Size: 213 30 566 30 882 30 160
Fragments arranged by size:
882 566 213 160 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 232 761 826 984 1911
Size: 145 87 529 65 158 927
Fragments arranged by size:
927 529 158 145 87 65
BseYI C'CCAG_C
Cuts at: 0 480 837 932 943 1069 1711 1754 1830
Size: 480 357 95 11 126 642 43 76
Cuts at: 1830 1911
Size: 81
Fragments arranged by size:
642 480 357 126 95 81 76 43
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1143 1911
Size: 559 584 768
Fragments arranged by size:
768 584 559
BsiHKAI G_wGCw'C
Cuts at: 0 1030 1911
Size: 1030 881
BsmI GAATG_Cn'
Cuts at: 0 1502 1911
Size: 1502 409
BsmBI CGTCTCn'nnnn_
Cuts at: 0 204 812 1911
Size: 204 608 1099
Fragments arranged by size:
1099 608 204
Bsp1286I G_dGCh'C
Cuts at: 0 510 535 551 753 771 806 880 922
Size: 510 25 16 202 18 35 74 42
Cuts at: 922 1030 1059 1076 1243 1487 1494 1570 1696
Size: 108 29 17 167 244 7 76 126
Cuts at: 1696 1711 1780 1911
Size: 15 69 131
Fragments arranged by size:
510 244 202 167 131 126 108 76
74 69 42 35 29 25 18 17
16 15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1110 1553 1911
Size: 1110 443 358
Fragments arranged by size:
1110 443 358
BsrBI CCG'CTC
Cuts at: 0 1274 1911
Size: 1274 637
BsrFI r'CCGG_y
Cuts at: 0 236 1763 1911
Size: 236 1527 148
Fragments arranged by size:
1527 236 148
BstAPI GCAn_nnn'nTGC
Cuts at: 0 505 546 1911
Size: 505 41 1365
Fragments arranged by size:
1365 505 41
BstBI TT'CG_AA
Cuts at: 0 1649 1911
Size: 1649 262
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 951 1044 1911
Size: 480 471 93 867
Fragments arranged by size:
867 480 471 93
BstYI r'GATC_y
Cuts at: 0 226 1911
Size: 226 1685
Bsu36I CC'TnA_GG
Cuts at: 0 27 128 855 1911
Size: 27 101 727 1056
Fragments arranged by size:
1056 727 101 27
BtgI C'CryG_G
Cuts at: 0 369 473 1911
Size: 369 104 1438
Fragments arranged by size:
1438 369 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 999 1241 1911
Size: 999 242 670
Fragments arranged by size:
999 670 242
BtsI GCAGTG_nn'
Cuts at: 0 561 1748 1911
Size: 561 1187 163
Fragments arranged by size:
1187 561 163
DrdI GACnn_nn'nnGTC
Cuts at: 0 600 1911
Size: 600 1311
EaeI y'GGCC_r
Cuts at: 0 693 1386 1702 1745 1911
Size: 693 693 316 43 166
Fragments arranged by size:
693 693 316 166 43
EarI CTCTTCn'nnn_
Cuts at: 0 344 1043 1911
Size: 344 699 868
Fragments arranged by size:
868 699 344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 594 1911
Size: 594 1317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 448 566 746 818 851 1081 1316
Size: 186 262 118 180 72 33 230 235
Cuts at: 1316 1462 1911
Size: 146 449
Fragments arranged by size:
449 262 235 230 186 180 146 118
72 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 612 854 866 1110 1911
Size: 466 146 242 12 244 801
Fragments arranged by size:
801 466 244 242 146 12
EcoO109I rG'GnC_Cy
Cuts at: 0 90 151 380 456 486 767 802 803
Size: 90 61 229 76 30 281 35 1
Cuts at: 803 859 877 1145 1240 1357 1693 1708 1788
Size: 56 18 268 95 117 336 15 80
Cuts at: 1788 1867 1890 1911
Size: 79 23 21
Fragments arranged by size:
336 281 268 229 117 95 90 80
79 76 61 56 35 30 23 21
18 15 1
HaeII r_GCGC'y
Cuts at: 0 416 1809 1911
Size: 416 1393 102
Fragments arranged by size:
1393 416 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 809 841 1501 1533 1911
Size: 619 32 158 32 660 32 378
Fragments arranged by size:
660 619 378 158 32 32 32
HincII GTy'rAC
Cuts at: 0 250 1911
Size: 250 1661
HindIII A'AGCT_T
Cuts at: 0 1796 1911
Size: 1796 115
MscI TGG'CCA
Cuts at: 0 695 1388 1747 1911
Size: 695 693 359 164
Fragments arranged by size:
695 693 359 164
MslI CAynn'nnrTG
Cuts at: 0 1724 1911
Size: 1724 187
MspA1I CmG'CkG
Cuts at: 0 435 1126 1911
Size: 435 691 785
Fragments arranged by size:
785 691 435
NaeI GCC'GGC
Cuts at: 0 238 1765 1911
Size: 238 1527 146
Fragments arranged by size:
1527 238 146
NgoMIV G'CCGG_C
Cuts at: 0 236 1763 1911
Size: 236 1527 148
Fragments arranged by size:
1527 236 148
NsiI A_TGCA'T
Cuts at: 0 1314 1911
Size: 1314 597
NspI r_CATG'y
Cuts at: 0 1723 1911
Size: 1723 188
PasI CC'CwG_GG
Cuts at: 0 450 915 1911
Size: 450 465 996
Fragments arranged by size:
996 465 450
PfoI T'CCnGG_A
Cuts at: 0 325 423 464 867 1478 1911
Size: 325 98 41 403 611 433
Fragments arranged by size:
611 433 403 325 98 41
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1391 1423 1911
Size: 1391 32 488
Fragments arranged by size:
1391 488 32
PpuMI rG'GwC_Cy
Cuts at: 0 90 151 456 486 1357 1788 1890 1911
Size: 90 61 305 30 871 431 102 21
Fragments arranged by size:
871 431 305 102 90 61 30 21
PshAI GACnn'nnGTC
Cuts at: 0 1325 1911
Size: 1325 586
PspOMI G'GGCC_C
Cuts at: 0 767 802 876 1072 1239 1692 1707 1911
Size: 767 35 74 196 167 453 15 204
Fragments arranged by size:
767 453 204 196 167 74 35 15
PspXI vC'TCGA_Gb
Cuts at: 0 1408 1883 1911
Size: 1408 475 28
Fragments arranged by size:
1408 475 28
PstI C_TGCA'G
Cuts at: 0 1560 1911
Size: 1560 351
PvuII CAG'CTG
Cuts at: 0 435 1911
Size: 435 1476
SanDI GG'GwC_CC
Cuts at: 0 90 456 486 1890 1911
Size: 90 366 30 1404 21
Fragments arranged by size:
1404 366 90 30 21
SfcI C'TryA_G
Cuts at: 0 1244 1556 1911
Size: 1244 312 355
Fragments arranged by size:
1244 355 312
SmaI CCC'GGG
Cuts at: 0 903 1488 1911
Size: 903 585 423
Fragments arranged by size:
903 585 423
SmlI C'TyrA_G
Cuts at: 0 1364 1408 1883 1911
Size: 1364 44 475 28
Fragments arranged by size:
1364 475 44 28
StyI C'CwwG_G
Cuts at: 0 67 1748 1862 1911
Size: 67 1681 114 49
Fragments arranged by size:
1681 114 67 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 1795 1911
Size: 1279 516 116
Fragments arranged by size:
1279 516 116
Tth111I GACn'n_nGTC
Cuts at: 0 41 823 1911
Size: 41 782 1088
Fragments arranged by size:
1088 782 41
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1521 1911
Size: 1521 390
XhoI C'TCGA_G
Cuts at: 0 1408 1883 1911
Size: 1408 475 28
Fragments arranged by size:
1408 475 28
XmaI C'CCGG_G
Cuts at: 0 901 1486 1911
Size: 901 585 425
Fragments arranged by size:
901 585 425
XmnI GAAnn'nnTTC
Cuts at: 0 292 1911
Size: 292 1619
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI AvaI
BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII MscI MslI
MspA1I NaeI NgoMIV NsiI NspI PasI PfoI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SanDI SfcI
SmaI SmlI StyI TaqII Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BglII BmgBI BmtI
BsaAI BsaBI BsiEI BsiWI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DraIII
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI PacI PciI PflMI PmeI
PmlI PsiI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI ZraI