Restriction map for
pFN21AE9684
(Linear) (Six-base) MAPSORT of: KIEE9684.seq Check: 4909 from: 1 to: 2514
KIEE9684.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1970 2514
Size: 1970 544
AatII G_ACGT'C
Cuts at: 0 1782 2514
Size: 1782 732
AccI GT'mk_AC
Cuts at: 0 1716 2514
Size: 1716 798
Acc65I G'GTAC_C
Cuts at: 0 2288 2514
Size: 2288 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1557 2257 2320 2514
Size: 1557 700 63 194
Fragments arranged by size:
1557 700 194 63
AhdI GACnn_n'nnGTC
Cuts at: 0 947 1266 2514
Size: 947 319 1248
Fragments arranged by size:
1248 947 319
AleI CACnn'nnGTG
Cuts at: 0 844 1417 2514
Size: 844 573 1097
Fragments arranged by size:
1097 844 573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 2514
Size: 862 1652
AlwNI CAG_nnn'CTG
Cuts at: 0 801 1330 1389 2427 2480 2514
Size: 801 529 59 1038 53 34
Fragments arranged by size:
1038 801 529 59 53 34
ApaI G_GGCC'C
Cuts at: 0 1876 2514
Size: 1876 638
ApaLI G'TGCA_C
Cuts at: 0 878 2514
Size: 878 1636
ApoI r'AATT_y
Cuts at: 0 347 1015 2514
Size: 347 668 1499
Fragments arranged by size:
1499 668 347
AvaI C'yCGr_G
Cuts at: 0 990 1196 2514
Size: 990 206 1318
Fragments arranged by size:
1318 990 206
BamHI G'GATC_C
Cuts at: 0 186 2514
Size: 186 2328
BanI G'GyrC_C
Cuts at: 0 439 838 1033 1050 2288 2514
Size: 439 399 195 17 1238 226
Fragments arranged by size:
1238 439 399 226 195 17
BanII G_rGCy'C
Cuts at: 0 481 1204 1876 1994 2514
Size: 481 723 672 118 520
Fragments arranged by size:
723 672 520 481 118
BbeI G_GCGC'C
Cuts at: 0 1037 2514
Size: 1037 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 614 2440 2455 2514
Size: 614 1826 15 59
Fragments arranged by size:
1826 614 59 15
BbvCI CC'TCA_GC
Cuts at: 0 1401 1586 1972 2514
Size: 1401 185 386 542
Fragments arranged by size:
1401 542 386 185
BglI GCCn_nnn'nGGC
Cuts at: 0 2302 2389 2514
Size: 2302 87 125
Fragments arranged by size:
2302 125 87
BglII A'GATC_T
Cuts at: 0 2139 2514
Size: 2139 375
Bme1580I G_kGCm'C
Cuts at: 0 444 841 882 1053 1526 1727 1876 2514
Size: 444 397 41 171 473 201 149 638
Fragments arranged by size:
638 473 444 397 201 171 149 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 1078 1227 2023 2514
Size: 1078 149 796 491
Fragments arranged by size:
1078 796 491 149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1788 2514
Size: 1788 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 494 1642 1894 2371 2449 2514
Size: 405 89 1148 252 477 78 65
Fragments arranged by size:
1148 477 405 252 89 78 65
Bpu10I CC'TnA_GC
Cuts at: 0 533 1401 1586 1972 2514
Size: 533 868 185 386 542
Fragments arranged by size:
868 542 533 386 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 447 1072 1119 1666 1680 2514
Size: 253 194 625 47 547 14 834
Fragments arranged by size:
834 625 547 253 194 47 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1300 1432 1571 1732 2514
Size: 592 708 132 139 161 782
Fragments arranged by size:
782 708 592 161 139 132
BsaBI GATnn'nnATC
Cuts at: 0 2416 2514
Size: 2416 98
BsaHI Gr'CG_yC
Cuts at: 0 1034 1779 2514
Size: 1034 745 735
Fragments arranged by size:
1034 745 735
BsaWI w'CCGG_w
Cuts at: 0 88 1888 2354 2514
Size: 88 1800 466 160
Fragments arranged by size:
1800 466 160 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 735 765 876 906 1968 1998 2479 2509
Size: 735 30 111 30 1062 30 481 30
Cuts at: 2509 2514
Size: 5
Fragments arranged by size:
1062 735 481 111 30 30 30 30
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 797 978 1008 1159 1375 1579 1972
Size: 712 85 181 30 151 216 204 393
Cuts at: 1972 1989 2002 2329 2353 2479 2514
Size: 17 13 327 24 126 35
Fragments arranged by size:
712 393 327 216 204 181 151 126
85 35 30 24 17 13
BseYI C'CCAG_C
Cuts at: 0 399 629 1286 1314 1345 2514
Size: 399 230 657 28 31 1169
Fragments arranged by size:
1169 657 399 230 31 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 2514
Size: 1444 1070
BsiHKAI G_wGCw'C
Cuts at: 0 729 789 882 904 1824 1994 2514
Size: 729 60 93 22 920 170 520
Fragments arranged by size:
920 729 520 170 93 60 22
BsmI GAATG_Cn'
Cuts at: 0 673 709 1392 2514
Size: 673 36 683 1122
Fragments arranged by size:
1122 683 673 36
Bsp1286I G_dGCh'C
Cuts at: 0 444 481 729 789 841 882 904 1053
Size: 444 37 248 60 52 41 22 149
Cuts at: 1053 1204 1526 1727 1824 1876 1994 2514
Size: 151 322 201 97 52 118 520
Fragments arranged by size:
520 444 322 248 201 151 149 118
97 60 52 52 41 37 22
BspEI T'CCGG_A
Cuts at: 0 2354 2514
Size: 2354 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1913 1970 2514
Size: 1913 57 544
Fragments arranged by size:
1913 544 57
BsrBI CCG'CTC
Cuts at: 0 998 1163 1168 2514
Size: 998 165 5 1346
Fragments arranged by size:
1346 998 165 5
BsrDI GCAATG_nn'
Cuts at: 0 42 2514
Size: 42 2472
BsrFI r'CCGG_y
Cuts at: 0 707 2514
Size: 707 1807
BstAPI GCAn_nnn'nTGC
Cuts at: 0 801 2514
Size: 801 1713
BstBI TT'CG_AA
Cuts at: 0 2036 2514
Size: 2036 478
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 921 1800 2514
Size: 144 777 879 714
Fragments arranged by size:
879 777 714 144
BstYI r'GATC_y
Cuts at: 0 186 2139 2477 2514
Size: 186 1953 338 37
Fragments arranged by size:
1953 338 186 37
Bsu36I CC'TnA_GG
Cuts at: 0 767 953 1699 1773 2398 2514
Size: 767 186 746 74 625 116
Fragments arranged by size:
767 746 625 186 116 74
BtgI C'CryG_G
Cuts at: 0 542 1355 1727 1783 1995 2057 2150 2514
Size: 542 813 372 56 212 62 93 364
Fragments arranged by size:
813 542 372 364 212 93 62 56
BtsI GCAGTG_nn'
Cuts at: 0 743 1118 1284 1518 1943 2514
Size: 743 375 166 234 425 571
Fragments arranged by size:
743 571 425 375 234 166
DraIII CAC_nnn'GTG
Cuts at: 0 924 2514
Size: 924 1590
DrdI GACnn_nn'nnGTC
Cuts at: 0 28 2514
Size: 28 2486
EaeI y'GGCC_r
Cuts at: 0 146 221 740 980 1730 2514
Size: 146 75 519 240 750 784
Fragments arranged by size:
784 750 519 240 146 75
EarI CTCTTCn'nnn_
Cuts at: 0 474 573 2033 2179 2222 2514
Size: 474 99 1460 146 43 292
Fragments arranged by size:
1460 474 292 146 99 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2386 2514
Size: 2386 128
EcoICRI GAG'CTC
Cuts at: 0 1992 2514
Size: 1992 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 494 1557 1642 1894 2257 2320 2371
Size: 405 89 1063 85 252 363 63 51
Cuts at: 2371 2449 2514
Size: 78 65
Fragments arranged by size:
1063 405 363 252 89 85 78 65
63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1838 2401 2514
Size: 1838 563 113
Fragments arranged by size:
1838 563 113
EcoO109I rG'GnC_Cy
Cuts at: 0 1128 1176 1369 1872 1926 2514
Size: 1128 48 193 503 54 588
Fragments arranged by size:
1128 588 503 193 54 48
EcoRI G'AATT_C
Cuts at: 0 347 2514
Size: 347 2167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1113 1689 1813 2146 2514
Size: 1113 576 124 333 368
Fragments arranged by size:
1113 576 368 333 124
FspI TGC'GCA
Cuts at: 0 2416 2514
Size: 2416 98
FspAI rTGC'GCAy
Cuts at: 0 2416 2514
Size: 2416 98
HaeII r_GCGC'y
Cuts at: 0 1037 2514
Size: 1037 1477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 195 227 933 965 1252 1284 1692 1724
Size: 195 32 706 32 287 32 408 32
Cuts at: 1724 2131 2163 2479 2511 2514
Size: 407 32 316 32 3
Fragments arranged by size:
706 408 407 316 287 195 32 32
32 32 32 32 3
HindIII A'AGCT_T
Cuts at: 0 1795 2514
Size: 1795 719
KasI G'GCGC_C
Cuts at: 0 1033 2514
Size: 1033 1481
KpnI G_GTAC'C
Cuts at: 0 2292 2514
Size: 2292 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2192 2514
Size: 2192 322
MscI TGG'CCA
Cuts at: 0 148 223 742 982 1732 2514
Size: 148 75 519 240 750 782
Fragments arranged by size:
782 750 519 240 148 75
MslI CAynn'nnrTG
Cuts at: 0 844 1417 1834 2514
Size: 844 573 417 680
Fragments arranged by size:
844 680 573 417
MspA1I CmG'CkG
Cuts at: 0 1327 1389 1405 1585 1785 2328 2514
Size: 1327 62 16 180 200 543 186
Fragments arranged by size:
1327 543 200 186 180 62 16
NaeI GCC'GGC
Cuts at: 0 709 2514
Size: 709 1805
NarI GG'CG_CC
Cuts at: 0 1034 2514
Size: 1034 1480
NcoI C'CATG_G
Cuts at: 0 2057 2150 2514
Size: 2057 93 364
Fragments arranged by size:
2057 364 93
NgoMIV G'CCGG_C
Cuts at: 0 707 2514
Size: 707 1807
NspI r_CATG'y
Cuts at: 0 1345 1752 2514
Size: 1345 407 762
Fragments arranged by size:
1345 762 407
PasI CC'CwG_GG
Cuts at: 0 816 2514
Size: 816 1698
PflMI CCAn_nnn'nTGG
Cuts at: 0 406 924 2058 2427 2514
Size: 406 518 1134 369 87
Fragments arranged by size:
1134 518 406 369 87
PfoI T'CCnGG_A
Cuts at: 0 510 2514
Size: 510 2004
PpuMI rG'GwC_Cy
Cuts at: 0 1176 1369 1926 2514
Size: 1176 193 557 588
Fragments arranged by size:
1176 588 557 193
PspOMI G'GGCC_C
Cuts at: 0 1872 2514
Size: 1872 642
PspXI vC'TCGA_Gb
Cuts at: 0 990 2514
Size: 990 1524
PstI C_TGCA'G
Cuts at: 0 11 797 972 2327 2514
Size: 11 786 175 1355 187
Fragments arranged by size:
1355 786 187 175 11
PvuII CAG'CTG
Cuts at: 0 1389 2328 2514
Size: 1389 939 186
Fragments arranged by size:
1389 939 186
SacI G_AGCT'C
Cuts at: 0 1994 2514
Size: 1994 520
SacII CC_GC'GG
Cuts at: 0 1786 2514
Size: 1786 728
SapI GCTCTTCn'nnn_
Cuts at: 0 474 2514
Size: 474 2040
SbfI CC_TGCA'GG
Cuts at: 0 797 2514
Size: 797 1717
SexAI A'CCwGG_T
Cuts at: 0 920 2514
Size: 920 1594
SfcI C'TryA_G
Cuts at: 0 7 352 793 968 1895 2323 2514
Size: 7 345 441 175 927 428 191
Fragments arranged by size:
927 441 428 345 191 175 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2389 2514
Size: 2389 125
SfoI GGC'GCC
Cuts at: 0 1035 2514
Size: 1035 1479
SmlI C'TyrA_G
Cuts at: 0 232 462 990 1087 1098 1659 1681 2514
Size: 232 230 528 97 11 561 22 833
Fragments arranged by size:
833 561 528 232 230 97 22 11
SphI G_CATG'C
Cuts at: 0 1345 1752 2514
Size: 1345 407 762
Fragments arranged by size:
1345 762 407
StyI C'CwwG_G
Cuts at: 0 261 1060 1550 2057 2150 2514
Size: 261 799 490 507 93 364
Fragments arranged by size:
799 507 490 364 261 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 1543 2287 2514
Size: 150 1393 744 227
Fragments arranged by size:
1393 744 227 150
TatI w'GTAC_w
Cuts at: 0 1913 2514
Size: 1913 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 735 767 876 908 1476 1488 1508 1520
Size: 735 32 109 32 568 12 20 12
Cuts at: 1520 1883 1915 1968 2000 2514
Size: 363 32 53 32 514
Fragments arranged by size:
735 568 514 363 109 53 32 32
32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 0 624 2514
Size: 624 1890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 925 2514
Size: 921 4 1589
Fragments arranged by size:
1589 921 4
XhoI C'TCGA_G
Cuts at: 0 990 2514
Size: 990 1524
XmnI GAAnn'nnTTC
Cuts at: 0 2161 2514
Size: 2161 353
ZraI GAC'GTC
Cuts at: 0 1780 2514
Size: 1780 734
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI
SbfI SexAI SfcI SfiI SfoI SmlI SphI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AloI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BsaAI BsiEI BsiWI BsmBI BspHI BsrGI
BssHII BssSI BstEII BstZ17I BtgZI ClaI CspCI DraI
EagI EcoRV FseI HincII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI
ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI XbaI XmaI