(Linear) (Six-base) MAPSORT of: KIEE9682.seq  Check: 4155  from: 1  to: 969



KIEE9682.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     788     969 
   Size:        541     247     181

  Fragments arranged by size: 

                 541     247     181
 
ApoI r'AATT_y

Cuts at:      0     341     413     969 
   Size:        341      72     556

  Fragments arranged by size: 

                 556     341      72
 
AvaI C'yCGr_G

Cuts at:      0      14     969 
   Size:         14     955
 
BamHI G'GATC_C

Cuts at:      0     579     969 
   Size:        579     390
 
BanII G_rGCy'C

Cuts at:      0     112     377     969 
   Size:        112     265     592

  Fragments arranged by size: 

                 592     265     112
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     161     671     783     969 
   Size:        161     510     112     186

  Fragments arranged by size: 

                 510     186     161     112
 
BglII A'GATC_T

Cuts at:      0     648     763     969 
   Size:        648     115     206

  Fragments arranged by size: 

                 648     206     115
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      25     123     969 
   Size:         25      98     846

  Fragments arranged by size: 

                 846      98      25
 
BsaAI yAC'GTr

Cuts at:      0     808     969 
   Size:        808     161
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     499     969 
   Size:        499     470
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     969 
   Size:        736     233
 
BsiHKAI G_wGCw'C

Cuts at:      0     112     377     969 
   Size:        112     265     592

  Fragments arranged by size: 

                 592     265     112
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     377     969 
   Size:        112     265     592

  Fragments arranged by size: 

                 592     265     112
 
BsrDI GCAATG_nn'

Cuts at:      0     695     969 
   Size:        695     274
 
BstYI r'GATC_y

Cuts at:      0     579     648     763     969 
   Size:        579      69     115     206

  Fragments arranged by size: 

                 579     206     115      69
 
DraIII CAC_nnn'GTG

Cuts at:      0     714     969 
   Size:        714     255
 
EarI CTCTTCn'nnn_

Cuts at:      0     500     764     969 
   Size:        500     264     205

  Fragments arranged by size: 

                 500     264     205
 
EcoICRI GAG'CTC

Cuts at:      0     110     375     969 
   Size:        110     265     594

  Fragments arranged by size: 

                 594     265     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     788     969 
   Size:        541     247     181

  Fragments arranged by size: 

                 541     247     181
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     250     969 
   Size:        250     719
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56      97     129     178     210     517     544 
   Size:         24      32      41      32      49      32     307      27

Cuts at:    544     549     564     576     596     969 
   Size:          5      15      12      20     373

  Fragments arranged by size: 

                 373     307      49      41      32      32      32      27
                  24      20      15      12       5
 
HindIII A'AGCT_T

Cuts at:      0     419     919     969 
   Size:        419     500      50

  Fragments arranged by size: 

                 500     419      50
 
MslI CAynn'nnrTG

Cuts at:      0      44     969 
   Size:         44     925
 
MspA1I CmG'CkG

Cuts at:      0     315     969 
   Size:        315     654
 
PsiI TTA'TAA

Cuts at:      0     239     969 
   Size:        239     730
 
SacI G_AGCT'C

Cuts at:      0     112     377     969 
   Size:        112     265     592

  Fragments arranged by size: 

                 592     265     112
 
SfcI C'TryA_G

Cuts at:      0     366     937     969 
   Size:        366     571      32

  Fragments arranged by size: 

                 571     366      32
 
SspI AAT'ATT

Cuts at:      0     952     969 
   Size:        952      17
 
StuI AGG'CCT

Cuts at:      0     900     969 
   Size:        900      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     105     969 
   Size:        105     864
 
XmnI GAAnn'nnTTC

Cuts at:      0     951     969 
   Size:        951      18
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BamHI    BanII     BbsI    BglII     BsaI
    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I    BsrDI    BstYI   DraIII
     EarI  EcoICRI  Eco57MI     FalI    Hin4I  HindIII     MslI   MspA1I
     PsiI     SacI     SfcI     SspI     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI