Restriction map for
pFN21AE9679
(Linear) (Six-base) MAPSORT of: KIEE9679.seq Check: 198 from: 1 to: 8646
KIEE9679.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AccI GT'mk_AC
Cuts at: 0 855 2075 2324 3908 8646
Size: 855 1220 249 1584 4738
Fragments arranged by size:
4738 1584 1220 855 249
Acc65I G'GTAC_C
Cuts at: 0 4983 8646
Size: 4983 3663
AclI AA'CG_TT
Cuts at: 0 2801 8646
Size: 2801 5845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 844 1213 1655 1817 1982 2847 3193
Size: 695 149 369 442 162 165 865 346
Cuts at: 3193 3841 4424 4700 6197 6500 6830 7954 8646
Size: 648 583 276 1497 303 330 1124 692
Fragments arranged by size:
1497 1124 865 695 692 648 583 442
369 346 330 303 276 165 162 149
AflII C'TTAA_G
Cuts at: 0 3135 7492 8646
Size: 3135 4357 1154
Fragments arranged by size:
4357 3135 1154
AflIII A'CryG_T
Cuts at: 0 273 1750 3814 5347 7632 8646
Size: 273 1477 2064 1533 2285 1014
Fragments arranged by size:
2285 2064 1533 1477 1014 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7061 8033 8646
Size: 7061 972 613
Fragments arranged by size:
7061 972 613
AlwNI CAG_nnn'CTG
Cuts at: 0 94 1090 4729 5155 6412 6730 7891 7894
Size: 94 996 3639 426 1257 318 1161 3
Cuts at: 7894 8007 8646
Size: 113 639
Fragments arranged by size:
3639 1257 1161 996 639 426 318 113
94 3
ApaLI G'TGCA_C
Cuts at: 0 1069 8646
Size: 1069 7577
ApoI r'AATT_y
Cuts at: 0 117 130 232 570 591 750 996 1164
Size: 117 13 102 338 21 159 246 168
Cuts at: 1164 1998 2081 2143 2776 3169 3478 4117 5399
Size: 834 83 62 633 393 309 639 1282
Cuts at: 5399 5602 7391 7722 8646
Size: 203 1789 331 924
Fragments arranged by size:
1789 1282 924 834 639 633 393 338
331 309 246 203 168 159 117 102
83 62 21 13
AseI AT'TA_AT
Cuts at: 0 7424 8646
Size: 7424 1222
AvaI C'yCGr_G
Cuts at: 0 1216 3773 4819 7503 8646
Size: 1216 2557 1046 2684 1143
Fragments arranged by size:
2684 2557 1216 1143 1046
AvrII C'CTAG_G
Cuts at: 0 6094 8646
Size: 6094 2552
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3371 3404 8646
Size: 3371 33 5242
Fragments arranged by size:
5242 3371 33
BamHI G'GATC_C
Cuts at: 0 2222 5721 8646
Size: 2222 3499 2925
Fragments arranged by size:
3499 2925 2222
BanI G'GyrC_C
Cuts at: 0 3076 3270 4983 5427 6961 7201 8646
Size: 3076 194 1713 444 1534 240 1445
Fragments arranged by size:
3076 1713 1534 1445 444 240 194
BanII G_rGCy'C
Cuts at: 0 1651 3419 4602 6941 8646
Size: 1651 1768 1183 2339 1705
Fragments arranged by size:
2339 1768 1705 1651 1183
BbsI GAAGACnn'nnnn_
Cuts at: 0 1231 1777 1823 3106 5689 6164 6185 7079
Size: 1231 546 46 1283 2583 475 21 894
Cuts at: 7079 7566 8180 8519 8646
Size: 487 614 339 127
Fragments arranged by size:
2583 1283 1231 894 614 546 487 475
339 127 46 21
BbvCI CC'TCA_GC
Cuts at: 0 3458 8646
Size: 3458 5188
BciVI GTATCCnnnnn_n'
Cuts at: 0 2355 8646
Size: 2355 6291
BclI T'GATC_A
Cuts at: 0 1125 6794 6984 8646
Size: 1125 5669 190 1662
Fragments arranged by size:
5669 1662 1125 190
BfrBI ATG'CAT
Cuts at: 0 76 450 655 8646
Size: 76 374 205 7991
Fragments arranged by size:
7991 374 205 76
BglII A'GATC_T
Cuts at: 0 2448 4059 5268 8646
Size: 2448 1611 1209 3378
Fragments arranged by size:
3378 2448 1611 1209
Bme1580I G_kGCm'C
Cuts at: 0 1073 8646
Size: 1073 7573
BmgBI CAC'GTC
Cuts at: 0 4189 8646
Size: 4189 4457
BmrI ACTGGGnnnn_n'
Cuts at: 0 2775 3979 6935 8409 8646
Size: 2775 1204 2956 1474 237
Fragments arranged by size:
2956 2775 1474 1204 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2141 3661 6287 6935 7643 8075 8366 8646
Size: 2141 1520 2626 648 708 432 291 280
Fragments arranged by size:
2626 2141 1520 708 648 432 291 280
Bpu10I CC'TnA_GC
Cuts at: 0 3418 3458 8646
Size: 3418 40 5188
Fragments arranged by size:
5188 3418 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7370 8646
Size: 7370 1276
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 4437 6263 7129 8646
Size: 43 4394 1826 866 1517
Fragments arranged by size:
4394 1826 1517 866 43
BsaAI yAC'GTr
Cuts at: 0 2786 8646
Size: 2786 5860
BsaBI GATnn'nnATC
Cuts at: 0 35 2447 3515 3839 4136 6384 8646
Size: 35 2412 1068 324 297 2248 2262
Fragments arranged by size:
2412 2262 2248 1068 324 297 35
BsaHI Gr'CG_yC
Cuts at: 0 5358 8646
Size: 5358 3288
BsaWI w'CCGG_w
Cuts at: 0 4232 4439 8646
Size: 4232 207 4207
Fragments arranged by size:
4232 4207 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4434 4464 5258 5288 5676 5706 6083 6113
Size: 4434 30 794 30 388 30 377 30
Cuts at: 6113 6154 6184 7745 7775 8646
Size: 41 30 1561 30 871
Fragments arranged by size:
4434 1561 871 794 388 377 41 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2906 3475 4646 6068 6142 6175 6543 6546
Size: 2906 569 1171 1422 74 33 368 3
Cuts at: 6546 6549 6552 7326 8072 8646
Size: 3 3 774 746 574
Fragments arranged by size:
2906 1422 1171 774 746 574 569 368
74 33 3 3 3
BseYI C'CCAG_C
Cuts at: 0 264 5103 5220 6345 8020 8646
Size: 264 4839 117 1125 1675 626
Fragments arranged by size:
4839 1675 1125 626 264 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 8646
Size: 146 8500
BsiHKAI G_wGCw'C
Cuts at: 0 1073 2271 2404 3419 3605 4602 6676 7083
Size: 1073 1198 133 1015 186 997 2074 407
Cuts at: 7083 7502 8646
Size: 419 1144
Fragments arranged by size:
2074 1198 1144 1073 1015 997 419 407
186 133
BsiWI C'GTAC_G
Cuts at: 0 2782 8646
Size: 2782 5864
BsmI GAATG_Cn'
Cuts at: 0 1020 1389 4486 4901 5665 7547 8039 8646
Size: 1020 369 3097 415 764 1882 492 607
Fragments arranged by size:
3097 1882 1020 764 607 492 415 369
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3231 5365 7842 8646
Size: 3231 2134 2477 804
Fragments arranged by size:
3231 2477 2134 804
Bsp1286I G_dGCh'C
Cuts at: 0 1073 1651 2271 2404 3419 3605 4602 6676
Size: 1073 578 620 133 1015 186 997 2074
Cuts at: 6676 6941 7083 7502 8646
Size: 265 142 419 1144
Fragments arranged by size:
2074 1144 1073 1015 997 620 578 419
265 186 142 133
BspEI T'CCGG_A
Cuts at: 0 4439 8646
Size: 4439 4207
BspHI T'CATG_A
Cuts at: 0 1299 3044 5895 7551 8646
Size: 1299 1745 2851 1656 1095
Fragments arranged by size:
2851 1745 1656 1299 1095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4511 5223 8646
Size: 4511 712 3423
Fragments arranged by size:
4511 3423 712
BsrDI GCAATG_nn'
Cuts at: 0 2404 3533 8646
Size: 2404 1129 5113
Fragments arranged by size:
5113 2404 1129
BsrFI r'CCGG_y
Cuts at: 0 6964 8041 8646
Size: 6964 1077 605
Fragments arranged by size:
6964 1077 605
BsrGI T'GTAC_A
Cuts at: 0 81 8433 8646
Size: 81 8352 213
Fragments arranged by size:
8352 213 81
BssSI C'ACGA_G
Cuts at: 0 1637 4694 8646
Size: 1637 3057 3952
Fragments arranged by size:
3952 3057 1637
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 5155 6070 7891 7894 8007 8646
Size: 877 4278 915 1821 3 113 639
Fragments arranged by size:
4278 1821 915 877 639 113 3
BstBI TT'CG_AA
Cuts at: 0 4068 4806 8646
Size: 4068 738 3840
Fragments arranged by size:
4068 3840 738
BstXI CCAn_nnnn'nTGG
Cuts at: 0 334 3541 3883 5879 6280 8646
Size: 334 3207 342 1996 401 2366
Fragments arranged by size:
3207 2366 1996 401 342 334
BstYI r'GATC_y
Cuts at: 0 6 412 1086 2222 2448 3177 4059 4662
Size: 6 406 674 1136 226 729 882 603
Cuts at: 4662 5199 5268 5721 7956 8593 8646
Size: 537 69 453 2235 637 53
Fragments arranged by size:
2235 1136 882 729 674 637 603 537
453 406 226 69 53 6
Bsu36I CC'TnA_GG
Cuts at: 0 1034 8646
Size: 1034 7612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1487 2988 8646
Size: 1487 1501 5658
Fragments arranged by size:
5658 1501 1487
BtsI GCAGTG_nn'
Cuts at: 0 5366 8005 8646
Size: 5366 2639 641
Fragments arranged by size:
5366 2639 641
ClaI AT'CG_AT
Cuts at: 0 4136 8646
Size: 4136 4510
DraI TTT'AAA
Cuts at: 0 5064 5232 8646
Size: 5064 168 3414
Fragments arranged by size:
5064 3414 168
DraIII CAC_nnn'GTG
Cuts at: 0 3063 5934 8646
Size: 3063 2871 2712
Fragments arranged by size:
3063 2871 2712
DrdI GACnn_nn'nnGTC
Cuts at: 0 6726 8646
Size: 6726 1920
EaeI y'GGCC_r
Cuts at: 0 4548 6775 8646
Size: 4548 2227 1871
Fragments arranged by size:
4548 2227 1871
EarI CTCTTCn'nnn_
Cuts at: 0 1154 1462 1961 2192 2459 4002 4065 4121
Size: 1154 308 499 231 267 1543 63 56
Cuts at: 4121 5514 6465 6540 6552 6570 7973 8646
Size: 1393 951 75 12 18 1403 673
Fragments arranged by size:
1543 1403 1393 1154 951 673 499 308
267 231 75 63 56 18 12
EcoICRI GAG'CTC
Cuts at: 0 3417 4600 8646
Size: 3417 1183 4046
Fragments arranged by size:
4046 3417 1183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 844 1213 1655 1817 1982 2141 2847
Size: 695 149 369 442 162 165 159 706
Cuts at: 2847 3193 3661 3841 4424 4700 6197 6287 6500
Size: 346 468 180 583 276 1497 90 213
Cuts at: 6500 6830 6935 7643 7954 8075 8366 8646
Size: 330 105 708 311 121 291 280
Fragments arranged by size:
1497 708 706 695 583 468 442 369
346 330 311 291 280 276 213 180
165 162 159 149 121 105 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 941 1228 1231 5579 6157 8054 8646
Size: 941 287 3 4348 578 1897 592
Fragments arranged by size:
4348 1897 941 592 578 287 3
EcoO109I rG'GnC_Cy
Cuts at: 0 91 1221 2725 3033 5811 8646
Size: 91 1130 1504 308 2778 2835
Fragments arranged by size:
2835 2778 1504 1130 308 91
EcoRI G'AATT_C
Cuts at: 0 570 996 1998 7391 8646
Size: 570 426 1002 5393 1255
Fragments arranged by size:
5393 1255 1002 570 426
EcoRV GAT'ATC
Cuts at: 0 4134 8646
Size: 4134 4512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2221 2744 3161 3319 4081 4094 5485 6082
Size: 2221 523 417 158 762 13 1391 597
Cuts at: 6082 8291 8646
Size: 2209 355
Fragments arranged by size:
2221 2209 1391 762 597 523 417 355
158 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1828 1860 1873 1905 2727 2759 4096 4128
Size: 1828 32 13 32 822 32 1337 32
Cuts at: 4128 4525 4527 4557 4559 5242 5274 5676 5708
Size: 397 2 30 2 683 32 402 32
Cuts at: 5708 5760 5792 6154 6186 6262 6294 6388 6406
Size: 52 32 362 32 76 32 94 18
Cuts at: 6406 6420 6438 8443 8475 8572 8590 8604 8622
Size: 14 18 2005 32 97 18 14 18
Cuts at: 8622 8646
Size: 24
Fragments arranged by size:
2005 1828 1337 822 683 402 397 362
97 94 76 52 32 32 32 32
32 32 32 32 32 32 30 24
18 18 18 18 14 14 13 2
2
HincII GTy'rAC
Cuts at: 0 630 1095 1262 4196 5296 6992 7025 7278
Size: 630 465 167 2934 1100 1696 33 253
Cuts at: 7278 8646
Size: 1368
Fragments arranged by size:
2934 1696 1368 1100 630 465 253 167
33
HindIII A'AGCT_T
Cuts at: 0 462 2483 5845 8646
Size: 462 2021 3362 2801
Fragments arranged by size:
3362 2801 2021 462
HpaI GTT'AAC
Cuts at: 0 630 8646
Size: 630 8016
KpnI G_GTAC'C
Cuts at: 0 4987 8646
Size: 4987 3659
MfeI C'AATT_G
Cuts at: 0 2032 2069 2619 2963 4172 8646
Size: 2032 37 550 344 1209 4474
Fragments arranged by size:
4474 2032 1209 550 344 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 4699 5662 6785 7650 8428 8646
Size: 202 4497 963 1123 865 778 218
Fragments arranged by size:
4497 1123 963 865 778 218 202
MscI TGG'CCA
Cuts at: 0 6777 8646
Size: 6777 1869
MslI CAynn'nnrTG
Cuts at: 0 1121 1253 1418 5072 5674 8646
Size: 1121 132 165 3654 602 2972
Fragments arranged by size:
3654 2972 1121 602 165 132
MspA1I CmG'CkG
Cuts at: 0 268 3502 3808 7891 8308 8314 8646
Size: 268 3234 306 4083 417 6 332
Fragments arranged by size:
4083 3234 417 332 306 268 6
NdeI CA'TA_TG
Cuts at: 0 2412 3933 4657 8646
Size: 2412 1521 724 3989
Fragments arranged by size:
3989 2412 1521 724
NsiI A_TGCA'T
Cuts at: 0 78 452 657 8646
Size: 78 374 205 7989
Fragments arranged by size:
7989 374 205 78
NspI r_CATG'y
Cuts at: 0 277 1754 3818 7794 8646
Size: 277 1477 2064 3976 852
Fragments arranged by size:
3976 2064 1477 852 277
PasI CC'CwG_GG
Cuts at: 0 562 4736 8646
Size: 562 4174 3910
Fragments arranged by size:
4174 3910 562
PciI A'CATG_T
Cuts at: 0 273 1750 3814 8646
Size: 273 1477 2064 4832
Fragments arranged by size:
4832 2064 1477 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 4265 6244 7066 7198 8646
Size: 4265 1979 822 132 1448
Fragments arranged by size:
4265 1979 1448 822 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1461 1493 7790 7822 8220 8252 8646
Size: 1461 32 6297 32 398 32 394
Fragments arranged by size:
6297 1461 398 394 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 91 1221 2725 8646
Size: 91 1130 1504 5921
Fragments arranged by size:
5921 1504 1130 91
PshAI GACnn'nnGTC
Cuts at: 0 4191 8646
Size: 4191 4455
PsiI TTA'TAA
Cuts at: 0 3448 8646
Size: 3448 5198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2012 2044 8646
Size: 2012 32 6602
Fragments arranged by size:
6602 2012 32
PstI C_TGCA'G
Cuts at: 0 873 3807 8313 8646
Size: 873 2934 4506 333
Fragments arranged by size:
4506 2934 873 333
PvuII CAG'CTG
Cuts at: 0 268 3502 3808 7891 8308 8314 8646
Size: 268 3234 306 4083 417 6 332
Fragments arranged by size:
4083 3234 417 332 306 268 6
SacI G_AGCT'C
Cuts at: 0 3419 4602 8646
Size: 3419 1183 4044
Fragments arranged by size:
4044 3419 1183
SapI GCTCTTCn'nnn_
Cuts at: 0 1462 2192 8646
Size: 1462 730 6454
Fragments arranged by size:
6454 1462 730
ScaI AGT'ACT
Cuts at: 0 2424 6627 8646
Size: 2424 4203 2019
Fragments arranged by size:
4203 2424 2019
SexAI A'CCwGG_T
Cuts at: 0 2013 8646
Size: 2013 6633
SfcI C'TryA_G
Cuts at: 0 869 3719 3803 3909 4702 6000 6046 6923
Size: 869 2850 84 106 793 1298 46 877
Cuts at: 6923 7232 8309 8471 8646
Size: 309 1077 162 175
Fragments arranged by size:
2850 1298 1077 877 869 793 309 175
162 106 84 46
SmaI CCC'GGG
Cuts at: 0 7505 8646
Size: 7505 1141
SmlI C'TyrA_G
Cuts at: 0 3135 7385 7492 8646
Size: 3135 4250 107 1154
Fragments arranged by size:
4250 3135 1154 107
SpeI A'CTAG_T
Cuts at: 0 102 8646
Size: 102 8544
SspI AAT'ATT
Cuts at: 0 1016 1632 2529 3312 5656 5730 8646
Size: 1016 616 897 783 2344 74 2916
Fragments arranged by size:
2916 2344 1016 897 783 616 74
StuI AGG'CCT
Cuts at: 0 2493 4718 7975 8387 8646
Size: 2493 2225 3257 412 259
Fragments arranged by size:
3257 2493 2225 412 259
StyI C'CwwG_G
Cuts at: 0 4205 6094 7197 8646
Size: 4205 1889 1103 1449
Fragments arranged by size:
4205 1889 1449 1103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 650 3968 4505 8646
Size: 112 538 3318 537 4141
Fragments arranged by size:
4141 3318 538 537 112
TatI w'GTAC_w
Cuts at: 0 81 520 2422 2858 6625 8433 8602 8646
Size: 81 439 1902 436 3767 1808 169 44
Fragments arranged by size:
3767 1902 1808 439 436 169 81 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4434 4466 5457 5489 8646
Size: 4434 32 991 32 3157
Fragments arranged by size:
4434 3157 991 32 32
XbaI T'CTAG_A
Cuts at: 0 2092 6123 8646
Size: 2092 4031 2523
Fragments arranged by size:
4031 2523 2092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3966 5712 6280 6900 8646
Size: 3966 1746 568 620 1746
Fragments arranged by size:
3966 1746 1746 620 568
XmaI C'CCGG_G
Cuts at: 0 7503 8646
Size: 7503 1143
XmnI GAAnn'nnTTC
Cuts at: 0 924 1658 2705 2933 4802 7634 7802 8646
Size: 924 734 1047 228 1869 2832 168 844
Fragments arranged by size:
2832 1869 1047 924 844 734 228 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BciVI BclI BfrBI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I
NdeI NsiI NspI PasI PciI PflMI PpiI PpuMI
PshAI PsiI PsrI PstI PvuII SacI SapI ScaI
SexAI SfcI SmaI SmlI SpeI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AsiSI BbeI BcgI BglI BlpI BmtI BplI
BsiEI BsrBI BssHII BstEII BstZ17I BtgI CspCI EagI
EciI FseI FspI FspAI HaeII KasI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PfoI
PmeI PmlI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI SwaI Tth111I XhoI ZraI