Restriction map for
pFN21AE9674
(Linear) (Six-base) MAPSORT of: KIEE9674.seq Check: 9639 from: 1 to: 2862
KIEE9674.orf
With 182 enzymes: SgfI *
December 27, 2011 16:43 ..
AatII G_ACGT'C
Cuts at: 0 1949 2862
Size: 1949 913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 1298 1918 2138 2717 2862
Size: 819 479 620 220 579 145
Fragments arranged by size:
819 620 579 479 220 145
AhdI GACnn_n'nnGTC
Cuts at: 0 2798 2862
Size: 2798 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2102 2862
Size: 2102 760
AlwNI CAG_nnn'CTG
Cuts at: 0 903 2862
Size: 903 1959
ApaLI G'TGCA_C
Cuts at: 0 2452 2862
Size: 2452 410
ApoI r'AATT_y
Cuts at: 0 165 2824 2862
Size: 165 2659 38
Fragments arranged by size:
2659 165 38
AvaI C'yCGr_G
Cuts at: 0 111 2862
Size: 111 2751
BanI G'GyrC_C
Cuts at: 0 115 1452 1931 2862
Size: 115 1337 479 931
Fragments arranged by size:
1337 931 479 115
BanII G_rGCy'C
Cuts at: 0 1516 1769 2696 2862
Size: 1516 253 927 166
Fragments arranged by size:
1516 927 253 166
BbeI G_GCGC'C
Cuts at: 0 1935 2862
Size: 1935 927
BbsI GAAGACnn'nnnn_
Cuts at: 0 666 1237 1396 2862
Size: 666 571 159 1466
Fragments arranged by size:
1466 666 571 159
BbvCI CC'TCA_GC
Cuts at: 0 2441 2862
Size: 2441 421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1512 1546 2862
Size: 1512 34 1316
Fragments arranged by size:
1512 1316 34
BclI T'GATC_A
Cuts at: 0 1568 2862
Size: 1568 1294
BfrBI ATG'CAT
Cuts at: 0 1004 2862
Size: 1004 1858
BglII A'GATC_T
Cuts at: 0 2642 2862
Size: 2642 220
BlpI GC'TnA_GC
Cuts at: 0 2285 2405 2862
Size: 2285 120 457
Fragments arranged by size:
2285 457 120
Bme1580I G_kGCm'C
Cuts at: 0 118 858 1741 2456 2509 2862
Size: 118 740 883 715 53 353
Fragments arranged by size:
883 740 715 353 118 53
BmgBI CAC'GTC
Cuts at: 0 827 2862
Size: 827 2035
BmrI ACTGGGnnnn_n'
Cuts at: 0 1550 1803 2847 2862
Size: 1550 253 1044 15
Fragments arranged by size:
1550 1044 253 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 951 2862
Size: 951 1911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 649 999 1346 1930 2862
Size: 374 275 350 347 584 932
Fragments arranged by size:
932 584 374 350 347 275
Bpu10I CC'TnA_GC
Cuts at: 0 443 2441 2862
Size: 443 1998 421
Fragments arranged by size:
1998 443 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 1210 1921 2254 2862
Size: 435 775 711 333 608
Fragments arranged by size:
775 711 608 435 333
BsaI GGTCTCn'nnnn_
Cuts at: 0 2572 2862
Size: 2572 290
BsaAI yAC'GTr
Cuts at: 0 340 2247 2862
Size: 340 1907 615
Fragments arranged by size:
1907 615 340
BsaHI Gr'CG_yC
Cuts at: 0 1932 1946 2862
Size: 1932 14 916
Fragments arranged by size:
1932 916 14
BsaWI w'CCGG_w
Cuts at: 0 1424 2862
Size: 1424 1438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2284 2314 2862
Size: 2284 30 548
Fragments arranged by size:
2284 548 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 359 2370 2862
Size: 34 325 2011 492
Fragments arranged by size:
2011 492 325 34
BseYI C'CCAG_C
Cuts at: 0 796 1955 2862
Size: 796 1159 907
Fragments arranged by size:
1159 907 796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2269 2862
Size: 2269 593
BsiEI CG_ry'CG
Cuts at: 0 738 2862
Size: 738 2124
BsiHKAI G_wGCw'C
Cuts at: 0 1136 1769 2456 2696 2862
Size: 1136 633 687 240 166
Fragments arranged by size:
1136 687 633 240 166
BsmI GAATG_Cn'
Cuts at: 0 2483 2862
Size: 2483 379
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2858 2862
Size: 2858 4
Bsp1286I G_dGCh'C
Cuts at: 0 118 858 1136 1516 1741 1769 2456 2509
Size: 118 740 278 380 225 28 687 53
Cuts at: 2509 2696 2862
Size: 187 166
Fragments arranged by size:
740 687 380 278 225 187 166 118
53 28
BspHI T'CATG_A
Cuts at: 0 258 2862
Size: 258 2604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 902 2862
Size: 902 1960
BsrBI CCG'CTC
Cuts at: 0 1440 2289 2862
Size: 1440 849 573
Fragments arranged by size:
1440 849 573
BsrDI GCAATG_nn'
Cuts at: 0 122 773 2862
Size: 122 651 2089
Fragments arranged by size:
2089 651 122
BsrFI r'CCGG_y
Cuts at: 0 958 1698 2862
Size: 958 740 1164
Fragments arranged by size:
1164 958 740
BssSI C'ACGA_G
Cuts at: 0 1294 2494 2862
Size: 1294 1200 368
Fragments arranged by size:
1294 1200 368
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2478 2862
Size: 2478 384
BstYI r'GATC_y
Cuts at: 0 2642 2862
Size: 2642 220
BtgI C'CryG_G
Cuts at: 0 198 884 2477 2862
Size: 198 686 1593 385
Fragments arranged by size:
1593 686 385 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1014 1395 1767 2862
Size: 1014 381 372 1095
Fragments arranged by size:
1095 1014 381 372
BtsI GCAGTG_nn'
Cuts at: 0 785 951 2862
Size: 785 166 1911
Fragments arranged by size:
1911 785 166
ClaI AT'CG_AT
Cuts at: 0 2264 2862
Size: 2264 598
DraI TTT'AAA
Cuts at: 0 1071 1629 2862
Size: 1071 558 1233
Fragments arranged by size:
1233 1071 558
EaeI y'GGCC_r
Cuts at: 0 500 1435 2336 2862
Size: 500 935 901 526
Fragments arranged by size:
935 901 526 500
EarI CTCTTCn'nnn_
Cuts at: 0 35 2104 2166 2862
Size: 35 2069 62 696
Fragments arranged by size:
2069 696 62 35
EcoICRI GAG'CTC
Cuts at: 0 1767 2694 2862
Size: 1767 927 168
Fragments arranged by size:
1767 927 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 649 819 999 1298 1346 1918 1930
Size: 374 275 170 180 299 48 572 12
Cuts at: 1930 2138 2717 2862
Size: 208 579 145
Fragments arranged by size:
579 572 374 299 275 208 180 170
145 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1909 2620 2862
Size: 1909 711 242
Fragments arranged by size:
1909 711 242
EcoO109I rG'GnC_Cy
Cuts at: 0 396 680 1019 1053 2295 2625 2862
Size: 396 284 339 34 1242 330 237
Fragments arranged by size:
1242 396 339 330 284 237 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 197 1134 2587 2862
Size: 197 937 1453 275
Fragments arranged by size:
1453 937 275 197
FseI GG_CCGG'CC
Cuts at: 0 962 2862
Size: 962 1900
HaeII r_GCGC'y
Cuts at: 0 1935 2862
Size: 1935 927
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 992 1024 1082 1114 1313 1345
Size: 516 32 444 32 58 32 199 32
Cuts at: 1345 2770 2784 2802 2816 2862
Size: 1425 14 18 14 46
Fragments arranged by size:
1425 516 444 199 58 46 32 32
32 32 18 14 14
HincII GTy'rAC
Cuts at: 0 891 2862
Size: 891 1971
KasI G'GCGC_C
Cuts at: 0 1931 2862
Size: 1931 931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1583 1907 1967 2300 2337 2862
Size: 1583 324 60 333 37 525
Fragments arranged by size:
1583 525 333 324 60 37
MscI TGG'CCA
Cuts at: 0 502 2862
Size: 502 2360
MslI CAynn'nnrTG
Cuts at: 0 778 2862
Size: 778 2084
MspA1I CmG'CkG
Cuts at: 0 1179 1561 1682 1959 2116 2284 2356 2512
Size: 1179 382 121 277 157 168 72 156
Cuts at: 2512 2862
Size: 350
Fragments arranged by size:
1179 382 350 277 168 157 156 121
72
NaeI GCC'GGC
Cuts at: 0 960 2862
Size: 960 1902
NarI GG'CG_CC
Cuts at: 0 1932 2862
Size: 1932 930
NcoI C'CATG_G
Cuts at: 0 198 2862
Size: 198 2664
NgoMIV G'CCGG_C
Cuts at: 0 958 2862
Size: 958 1904
NsiI A_TGCA'T
Cuts at: 0 1006 2862
Size: 1006 1856
NspI r_CATG'y
Cuts at: 0 1008 2332 2862
Size: 1008 1324 530
Fragments arranged by size:
1324 1008 530
PasI CC'CwG_GG
Cuts at: 0 554 2422 2862
Size: 554 1868 440
Fragments arranged by size:
1868 554 440
PflMI CCAn_nnn'nTGG
Cuts at: 0 827 1112 2205 2763 2862
Size: 827 285 1093 558 99
Fragments arranged by size:
1093 827 558 285 99
PfoI T'CCnGG_A
Cuts at: 0 2210 2696 2862
Size: 2210 486 166
Fragments arranged by size:
2210 486 166
PmlI CAC'GTG
Cuts at: 0 2247 2862
Size: 2247 615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 2862
Size: 448 32 2382
Fragments arranged by size:
2382 448 32
PpuMI rG'GwC_Cy
Cuts at: 0 396 680 1053 2295 2625 2862
Size: 396 284 373 1242 330 237
Fragments arranged by size:
1242 396 373 330 284 237
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 598 630 2862
Size: 598 32 2232
Fragments arranged by size:
2232 598 32
PstI C_TGCA'G
Cuts at: 0 899 2040 2862
Size: 899 1141 822
Fragments arranged by size:
1141 899 822
PvuI CG_AT'CG
Cuts at: 0 738 2862
Size: 738 2124
PvuII CAG'CTG
Cuts at: 0 1179 1561 1959 2116 2284 2512 2862
Size: 1179 382 398 157 168 228 350
Fragments arranged by size:
1179 398 382 350 228 168 157
SacI G_AGCT'C
Cuts at: 0 1769 2696 2862
Size: 1769 927 166
Fragments arranged by size:
1769 927 166
SexAI A'CCwGG_T
Cuts at: 0 2390 2862
Size: 2390 472
SfcI C'TryA_G
Cuts at: 0 849 895 2036 2862
Size: 849 46 1141 826
Fragments arranged by size:
1141 849 826 46
SfoI GGC'GCC
Cuts at: 0 1933 2862
Size: 1933 929
SmlI C'TyrA_G
Cuts at: 0 414 1225 1936 2233 2862
Size: 414 811 711 297 629
Fragments arranged by size:
811 711 629 414 297
SphI G_CATG'C
Cuts at: 0 1008 2332 2862
Size: 1008 1324 530
Fragments arranged by size:
1324 1008 530
StuI AGG'CCT
Cuts at: 0 1486 2862
Size: 1486 1376
StyI C'CwwG_G
Cuts at: 0 172 198 374 1855 2042 2057 2862
Size: 172 26 176 1481 187 15 805
Fragments arranged by size:
1481 805 187 176 172 26 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2284 2316 2862
Size: 2284 32 546
Fragments arranged by size:
2284 546 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 751 2025 2862
Size: 751 1274 837
Fragments arranged by size:
1274 837 751
XmnI GAAnn'nnTTC
Cuts at: 0 2730 2751 2862
Size: 2730 21 111
Fragments arranged by size:
2730 111 21
ZraI GAC'GTC
Cuts at: 0 1947 2862
Size: 1947 915
Enzymes that do cut:
AatII AcuI AhdI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII SacI SexAI SfcI SfoI SmlI
SphI StuI StyI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BglI BmtI BsaBI BsiWI BspEI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraIII
DrdI EagI EciI EcoRI EcoRV FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
PacI PciI PmeI PshAI PsiI PspOMI PspXI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI