Restriction map for
pFN21AE9670
(Linear) (Six-base) MAPSORT of: KIEE9670.seq Check: 5148 from: 1 to: 9690
KIEE9670.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3171 3339 8154 8568 9690
Size: 3171 168 4815 414 1122
Fragments arranged by size:
4815 3171 1122 414 168
AatII G_ACGT'C
Cuts at: 0 9570 9690
Size: 9570 120
AccI GT'mk_AC
Cuts at: 0 4906 9690
Size: 4906 4784
Acc65I G'GTAC_C
Cuts at: 0 4941 5172 9690
Size: 4941 231 4518
Fragments arranged by size:
4941 4518 231
AclI AA'CG_TT
Cuts at: 0 2061 9690
Size: 2061 7629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1115 1127 1604 2751 4145 4169 5333
Size: 382 733 12 477 1147 1394 24 1164
Cuts at: 5333 5780 6698 7328 7931 7975 8099 9542 9690
Size: 447 918 630 603 44 124 1443 148
Fragments arranged by size:
1443 1394 1164 1147 918 733 630 603
477 447 382 148 124 44 24 12
AfeI AGC'GCT
Cuts at: 0 2272 6940 9690
Size: 2272 4668 2750
Fragments arranged by size:
4668 2750 2272
AflII C'TTAA_G
Cuts at: 0 6102 9690
Size: 6102 3588
AflIII A'CryG_T
Cuts at: 0 4596 9690
Size: 4596 5094
AgeI A'CCGG_T
Cuts at: 0 2730 4938 9690
Size: 2730 2208 4752
Fragments arranged by size:
4752 2730 2208
AhdI GACnn_n'nnGTC
Cuts at: 0 738 4562 5904 9690
Size: 738 3824 1342 3786
Fragments arranged by size:
3824 3786 1342 738
AleI CACnn'nnGTG
Cuts at: 0 2029 3472 4108 8329 9690
Size: 2029 1443 636 4221 1361
Fragments arranged by size:
4221 2029 1443 1361 636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1367 6920 8347 9690
Size: 1367 5553 1427 1343
Fragments arranged by size:
5553 1427 1367 1343
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5696 5728 7042 7074 9690
Size: 5696 32 1314 32 2616
Fragments arranged by size:
5696 2616 1314 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1375 1495 1771 3088 4852 5040 5193 5235
Size: 1375 120 276 1317 1764 188 153 42
Cuts at: 5235 5361 6394 6416 7387 7765 7879 7978 8617
Size: 126 1033 22 971 378 114 99 639
Cuts at: 8617 8686 8716 9690
Size: 69 30 974
Fragments arranged by size:
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276 188 153 126 120 114 99 69
42 30 22
ApaI G_GGCC'C
Cuts at: 0 259 549 1445 1724 3061 3173 4408 5284
Size: 259 290 896 279 1337 112 1235 876
Cuts at: 5284 5398 5739 8423 8747 9429 9690
Size: 114 341 2684 324 682 261
Fragments arranged by size:
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290 279 261 259 114 112
ApaLI G'TGCA_C
Cuts at: 0 3715 9690
Size: 3715 5975
ApoI r'AATT_y
Cuts at: 0 6808 6952 9690
Size: 6808 144 2738
Fragments arranged by size:
6808 2738 144
AvaI C'yCGr_G
Cuts at: 0 1082 1310 3950 4082 5016 6047 6473 6887
Size: 1082 228 2640 132 934 1031 426 414
Cuts at: 6887 7205 8361 8521 9097 9690
Size: 318 1156 160 576 593
Fragments arranged by size:
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414 318 228 160 132
AvrII C'CTAG_G
Cuts at: 0 7321 9690
Size: 7321 2369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5495 5528 9690
Size: 5495 33 4162
Fragments arranged by size:
5495 4162 33
BanI G'GyrC_C
Cuts at: 0 196 378 512 2066 2897 3048 3658 3891
Size: 196 182 134 1554 831 151 610 233
Cuts at: 3891 4440 4479 4647 4836 4941 5172 5202 5485
Size: 549 39 168 189 105 231 30 283
Cuts at: 5485 5742 5952 7129 7869 8637 9690
Size: 257 210 1177 740 768 1053
Fragments arranged by size:
1554 1177 1053 831 768 740 610 549
283 257 233 231 210 196 189 182
168 151 134 105 39 30
BanII G_rGCy'C
Cuts at: 0 259 389 549 1118 1445 1724 2103 3061
Size: 259 130 160 569 327 279 379 958
Cuts at: 3061 3095 3173 3352 4006 4089 4408 5023 5284
Size: 34 78 179 654 83 319 615 261
Cuts at: 5284 5374 5398 5739 6041 6054 6995 7206 7961
Size: 90 24 341 302 13 941 211 755
Cuts at: 7961 8423 8747 9429 9690
Size: 462 324 682 261
Fragments arranged by size:
958 941 755 682 654 615 569 462
379 341 327 324 319 302 279 261
261 259 211 179 160 130 90 83
78 34 24 13
BbeI G_GCGC'C
Cuts at: 0 3052 9690
Size: 3052 6638
BbsI GAAGACnn'nnnn_
Cuts at: 0 1951 2379 4538 7186 9690
Size: 1951 428 2159 2648 2504
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 29 807 1022 1256 3865 4929 6869 7883
Size: 29 778 215 234 2609 1064 1940 1014
Cuts at: 7883 7968 8087 9690
Size: 85 119 1603
Fragments arranged by size:
2609 1940 1603 1064 1014 778 234 215
119 85 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9345 9379 9690
Size: 9345 34 311
Fragments arranged by size:
9345 311 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 5523 9690
Size: 1236 4287 4167
Fragments arranged by size:
4287 4167 1236
BglI GCCn_nnn'nGGC
Cuts at: 0 1978 3577 3769 5807 5869 6775 8256 9690
Size: 1978 1599 192 2038 62 906 1481 1434
Fragments arranged by size:
2038 1978 1599 1481 1434 906 192 62
BglII A'GATC_T
Cuts at: 0 7749 9690
Size: 7749 1941
BlpI GC'TnA_GC
Cuts at: 0 332 1297 6797 6893 9690
Size: 332 965 5500 96 2797
Fragments arranged by size:
5500 2797 965 332 96
Bme1580I G_kGCm'C
Cuts at: 0 209 259 549 1445 1724 2071 3061 3173
Size: 209 50 290 896 279 347 990 112
Cuts at: 3173 3663 3719 4408 5284 5398 5739 6668 7604
Size: 490 56 689 876 114 341 929 936
Cuts at: 7604 7665 7742 8423 8747 9429 9690
Size: 61 77 681 324 682 261
Fragments arranged by size:
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490 347 341 324 290 279 261 209
114 112 77 61 56 50
BmgBI CAC'GTC
Cuts at: 0 6004 9690
Size: 6004 3686
BmrI ACTGGGnnnn_n'
Cuts at: 0 31 417 2007 2052 3061 3112 3331 5227
Size: 31 386 1590 45 1009 51 219 1896
Cuts at: 5227 5374 6084 7375 7387 8674 9424 9690
Size: 147 710 1291 12 1287 750 266
Fragments arranged by size:
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266 219 147 51 45 31 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2436 7009 8560 8658 9690
Size: 2436 4573 1551 98 1032
Fragments arranged by size:
4573 2436 1551 1032 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 232 636 753 801 1700 1765 2797
Size: 164 68 404 117 48 899 65 1032
Cuts at: 2797 3065 3347 4682 4706 4787 4895 5141 5165
Size: 268 282 1335 24 81 108 246 24
Cuts at: 5165 5231 5249 5258 5297 5686 6661 6742 6979
Size: 66 18 9 39 389 975 81 237
Cuts at: 6979 6991 7322 7526 7664 7711 7975 8023 8037
Size: 12 331 204 138 47 264 48 14
Cuts at: 8037 8591 9690
Size: 554 1099
Fragments arranged by size:
1335 1099 1032 975 899 554 404 389
331 282 268 264 246 237 204 164
138 117 108 81 81 68 66 65
48 48 47 39 24 24 18 14
12 9
Bpu10I CC'TnA_GC
Cuts at: 0 29 455 807 1022 1256 2788 3005 3089
Size: 29 426 352 215 234 1532 217 84
Cuts at: 3089 3346 3865 4703 4929 6437 6869 7883 7968
Size: 257 519 838 226 1508 432 1014 85
Cuts at: 7968 8087 9690
Size: 119 1603
Fragments arranged by size:
1603 1532 1508 1014 838 519 432 426
352 257 234 226 217 215 119 85
84 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 682 2104 4108 5388 6148 7202 7659
Size: 140 542 1422 2004 1280 760 1054 457
Cuts at: 7659 9690
Size: 2031
Fragments arranged by size:
2031 2004 1422 1280 1054 760 542 457
140
BsaI GGTCTCn'nnnn_
Cuts at: 0 8346 8609 8689 9336 9380 9690
Size: 8346 263 80 647 44 310
Fragments arranged by size:
8346 647 310 263 80 44
BsaAI yAC'GTr
Cuts at: 0 4206 9690
Size: 4206 5484
BsaBI GATnn'nnATC
Cuts at: 0 2804 9690
Size: 2804 6886
BsaHI Gr'CG_yC
Cuts at: 0 1822 2307 3049 5619 8163 9567 9690
Size: 1822 485 742 2570 2544 1404 123
Fragments arranged by size:
2570 2544 1822 1404 742 485 123
BsaWI w'CCGG_w
Cuts at: 0 1505 2730 4827 4881 4938 9690
Size: 1505 1225 2097 54 57 4752
Fragments arranged by size:
4752 2097 1505 1225 57 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2038 2068 2297 2310 2327 2340 2341 2371
Size: 2038 30 229 13 17 13 1 30
Cuts at: 2371 3402 3432 5295 5325 6348 6378 7042 7072
Size: 1031 30 1863 30 1023 30 664 30
Cuts at: 7072 7488 7518 7524 7554 9690
Size: 416 30 6 30 2136
Fragments arranged by size:
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30 30 30 30 30 30 30 30
17 13 13 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 571 1348 1454 1552 1642 2275 3068
Size: 535 36 777 106 98 90 633 793
Cuts at: 3068 3360 4187 4248 4799 4802 4811 6532 6697
Size: 292 827 61 551 3 9 1721 165
Cuts at: 6697 6892 6988 7003 7066 7090 7093 7096 7442
Size: 195 96 15 63 24 3 3 346
Cuts at: 7442 7493 7565 7713 7933 8147 9523 9550 9553
Size: 51 72 148 220 214 1376 27 3
Cuts at: 9553 9690
Size: 137
Fragments arranged by size:
1721 1376 827 793 777 633 551 535
346 292 220 214 195 165 148 137
106 98 96 90 72 63 61 51
36 27 24 15 9 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 111 2071 3101 3351 3838 3984 4347 4407
Size: 111 1960 1030 250 487 146 363 60
Cuts at: 4407 4452 4626 5052 5283 5289 5343 5427 5445
Size: 45 174 426 231 6 54 84 18
Cuts at: 5445 5572 5827 5885 7096 7819 8462 8496 8617
Size: 127 255 58 1211 723 643 34 121
Cuts at: 8617 8747 8762 9055 9690
Size: 130 15 293 635
Fragments arranged by size:
1960 1211 1030 723 643 635 487 426
363 293 255 250 231 174 146 130
127 121 111 84 60 58 54 45
34 18 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3476 5374 6785 7479 7799 9690
Size: 3476 1898 1411 694 320 1891
Fragments arranged by size:
3476 1898 1891 1411 694 320
BsiEI CG_ry'CG
Cuts at: 0 2731 5402 9690
Size: 2731 2671 4288
Fragments arranged by size:
4288 2731 2671
BsiHKAI G_wGCw'C
Cuts at: 0 3095 3719 4006 6041 6188 6995 7961 8541
Size: 3095 624 287 2035 147 807 966 580
Cuts at: 8541 9690
Size: 1149
Fragments arranged by size:
3095 2035 1149 966 807 624 580 287
147
BsmI GAATG_Cn'
Cuts at: 0 1916 6215 9230 9690
Size: 1916 4299 3015 460
Fragments arranged by size:
4299 3015 1916 460
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2298 8525 9690
Size: 2298 6227 1165
Fragments arranged by size:
6227 2298 1165
Bsp1286I G_dGCh'C
Cuts at: 0 209 259 389 549 1118 1445 1724 2071
Size: 209 50 130 160 569 327 279 347
Cuts at: 2071 2103 3061 3095 3173 3352 3663 3719 4006
Size: 32 958 34 78 179 311 56 287
Cuts at: 4006 4089 4408 5023 5284 5374 5398 5739 6041
Size: 83 319 615 261 90 24 341 302
Cuts at: 6041 6054 6188 6668 6995 7206 7604 7665 7742
Size: 13 134 480 327 211 398 61 77
Cuts at: 7742 7961 8423 8541 8747 9429 9690
Size: 219 462 118 206 682 261
Fragments arranged by size:
958 682 615 569 480 462 398 347
341 327 327 319 311 302 287 279
261 261 219 211 209 206 179 160
134 130 118 90 83 78 77 61
56 50 34 32 24 13
BspHI T'CATG_A
Cuts at: 0 1068 2660 9690
Size: 1068 1592 7030
Fragments arranged by size:
7030 1592 1068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 698 3171 3339 6266 8154 8568 9690
Size: 698 2473 168 2927 1888 414 1122
Fragments arranged by size:
2927 2473 1888 1122 698 414 168
BsrBI CCG'CTC
Cuts at: 0 5491 9690
Size: 5491 4199
BsrDI GCAATG_nn'
Cuts at: 0 627 939 1757 5554 7953 8474 9690
Size: 627 312 818 3797 2399 521 1216
Fragments arranged by size:
3797 2399 1216 818 627 521 312
BsrFI r'CCGG_y
Cuts at: 0 2730 4052 4938 6857 7136 9581 9690
Size: 2730 1322 886 1919 279 2445 109
Fragments arranged by size:
2730 2445 1919 1322 886 279 109
BssSI C'ACGA_G
Cuts at: 0 517 3721 7178 8834 9690
Size: 517 3204 3457 1656 856
Fragments arranged by size:
3457 3204 1656 856 517
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4732 5485 6335 7363 9690
Size: 4732 753 850 1028 2327
Fragments arranged by size:
4732 2327 1028 850 753
BstBI TT'CG_AA
Cuts at: 0 8241 9690
Size: 8241 1449
BstEII G'GTnAC_C
Cuts at: 0 1887 2733 4564 9690
Size: 1887 846 1831 5126
Fragments arranged by size:
5126 1887 1831 846
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1870 4758 5990 8755 9258 9690
Size: 1870 2888 1232 2765 503 432
Fragments arranged by size:
2888 2765 1870 1232 503 432
BstYI r'GATC_y
Cuts at: 0 6271 7749 7772 8712 9690
Size: 6271 1478 23 940 978
Fragments arranged by size:
6271 1478 978 940 23
Bsu36I CC'TnA_GG
Cuts at: 0 374 1121 1466 3540 7646 8132 9494 9528
Size: 374 747 345 2074 4106 486 1362 34
Cuts at: 9528 9690
Size: 162
Fragments arranged by size:
4106 2074 1362 747 486 374 345 162
34
BtgI C'CryG_G
Cuts at: 0 590 1289 1328 1445 2795 3043 3449 3785
Size: 590 699 39 117 1350 248 406 336
Cuts at: 3785 3902 4619 5507 6243 6428 9251 9690
Size: 117 717 888 736 185 2823 439
Fragments arranged by size:
2823 1350 888 736 717 699 590 439
406 336 248 185 117 117 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2383 4826 9690
Size: 2383 2443 4864
Fragments arranged by size:
4864 2443 2383
BtsI GCAGTG_nn'
Cuts at: 0 439 1389 1707 3220 3479 5585 6152 8446
Size: 439 950 318 1513 259 2106 567 2294
Cuts at: 8446 8539 8938 8954 9690
Size: 93 399 16 736
Fragments arranged by size:
2294 2106 1513 950 736 567 439 399
318 259 93 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1963 1998 9690
Size: 1963 35 7692
Fragments arranged by size:
7692 1963 35
DraI TTT'AAA
Cuts at: 0 2079 9690
Size: 2079 7611
DraIII CAC_nnn'GTG
Cuts at: 0 3723 6183 9690
Size: 3723 2460 3507
Fragments arranged by size:
3723 3507 2460
EaeI y'GGCC_r
Cuts at: 0 593 1802 4050 4526 4664 5399 6246 6465
Size: 593 1209 2248 476 138 735 847 219
Cuts at: 6465 6566 6698 6855 7706 8209 9690
Size: 101 132 157 851 503 1481
Fragments arranged by size:
2248 1481 1209 851 847 735 593 503
476 219 157 138 132 101
EagI C'GGCC_G
Cuts at: 0 5399 9690
Size: 5399 4291
EarI CTCTTCn'nnn_
Cuts at: 0 265 358 419 789 807 881 992 1106
Size: 265 93 61 370 18 74 111 114
Cuts at: 1106 1196 1214 1232 1451 1580 1619 1670 4124
Size: 90 18 18 219 129 39 51 2454
Cuts at: 4124 4197 4239 4596 4829 6541 6674 6683 6746
Size: 73 42 357 233 1712 133 9 63
Cuts at: 6746 7001 8018 9010 9232 9690
Size: 255 1017 992 222 458
Fragments arranged by size:
2454 1712 1017 992 458 370 357 265
255 233 222 219 133 129 114 111
93 90 74 73 63 61 51 42
39 18 18 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7051 7236 9690
Size: 7051 185 2454
Fragments arranged by size:
7051 2454 185
EcoICRI GAG'CTC
Cuts at: 0 3093 4004 6039 6993 7959 9690
Size: 3093 911 2035 954 966 1731
Fragments arranged by size:
3093 2035 1731 966 954 911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 232 382 636 753 801 1115 1127
Size: 164 68 150 254 117 48 314 12
Cuts at: 1127 1604 1700 1765 2751 2797 3065 3347 4145
Size: 477 96 65 986 46 268 282 798
Cuts at: 4145 4169 4682 4706 4787 4895 5141 5165 5231
Size: 24 513 24 81 108 246 24 66
Cuts at: 5231 5249 5258 5297 5333 5686 5780 6661 6698
Size: 18 9 39 36 353 94 881 37
Cuts at: 6698 6742 6979 6991 7322 7328 7526 7664 7711
Size: 44 237 12 331 6 198 138 47
Cuts at: 7711 7931 7975 8023 8037 8099 8591 9542 9690
Size: 220 44 48 14 62 492 951 148
Fragments arranged by size:
986 951 881 798 513 492 477 353
331 314 282 268 254 246 237 220
198 164 150 148 138 117 108 96
94 81 68 66 65 62 48 48
47 46 44 44 39 37 36 24
24 24 18 14 12 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1097 1715 2488 3080 3778 6532 9690
Size: 1097 618 773 592 698 2754 3158
Fragments arranged by size:
3158 2754 1097 773 698 618 592
EcoO109I rG'GnC_Cy
Cuts at: 0 184 255 1036 1441 1720 2002 3058 3085
Size: 184 71 781 405 279 282 1056 27
Cuts at: 3085 3170 3273 3284 5125 5735 5736 8015 8137
Size: 85 103 11 1841 610 1 2279 122
Cuts at: 8137 8227 8420 8585 8744 9425 9426 9490 9554
Size: 90 193 165 159 681 1 64 64
Cuts at: 9554 9690
Size: 136
Fragments arranged by size:
2279 1841 1056 781 681 610 405 282
279 193 184 165 159 136 122 103
90 85 71 64 64 27 11 1
1
EcoRV GAT'ATC
Cuts at: 0 6759 9690
Size: 6759 2931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1339 9690
Size: 1339 8351
FseI GG_CCGG'CC
Cuts at: 0 6861 9690
Size: 6861 2829
FspI TGC'GCA
Cuts at: 0 499 6085 9625 9690
Size: 499 5586 3540 65
Fragments arranged by size:
5586 3540 499 65
FspAI rTGC'GCAy
Cuts at: 0 499 6085 9690
Size: 499 5586 3605
Fragments arranged by size:
5586 3605 499
HaeII r_GCGC'y
Cuts at: 0 465 2223 2274 3052 3504 5422 6942 9635
Size: 465 1758 51 778 452 1918 1520 2693
Cuts at: 9635 9690
Size: 55
Fragments arranged by size:
2693 1918 1758 1520 778 465 452 55
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 724 756 1588 1620 1947 1979
Size: 306 32 386 32 832 32 327 32
Cuts at: 1979 2297 2310 2329 2341 2342 2373 4548 4580
Size: 318 13 19 12 1 31 2175 32
Cuts at: 4580 5223 5255 5295 5327 5890 5922 6173 6205
Size: 643 32 40 32 563 32 251 32
Cuts at: 6205 6868 6900 8002 8034 8866 8898 9690
Size: 663 32 1102 32 832 32 792
Fragments arranged by size:
2175 1102 832 832 792 663 643 563
386 327 318 306 251 40 32 32
32 32 32 32 32 32 32 32
32 32 31 19 13 12 1
HincII GTy'rAC
Cuts at: 0 2593 2982 4043 4418 9690
Size: 2593 389 1061 375 5272
Fragments arranged by size:
5272 2593 1061 389 375
HindIII A'AGCT_T
Cuts at: 0 1906 9690
Size: 1906 7784
HpaI GTT'AAC
Cuts at: 0 4043 9690
Size: 4043 5647
KasI G'GCGC_C
Cuts at: 0 3048 9690
Size: 3048 6642
KpnI G_GTAC'C
Cuts at: 0 4945 5176 9690
Size: 4945 231 4514
Fragments arranged by size:
4945 4514 231
MfeI C'AATT_G
Cuts at: 0 6025 9690
Size: 6025 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 448 1010 1109 1247 3508 4975 5158
Size: 41 407 562 99 138 2261 1467 183
Cuts at: 5158 5248 5739 8554 8872 9290 9690
Size: 90 491 2815 318 418 400
Fragments arranged by size:
2815 2261 1467 562 491 418 407 400
318 183 138 99 90 41
MscI TGG'CCA
Cuts at: 0 595 1804 4528 4666 6248 6467 6568 6700
Size: 595 1209 2724 138 1582 219 101 132
Cuts at: 6700 7708 9690
Size: 1008 1982
Fragments arranged by size:
2724 1982 1582 1209 1008 595 219 138
132 101
MslI CAynn'nnrTG
Cuts at: 0 1073 1187 2029 2680 3472 4108 6199 8329
Size: 1073 114 842 651 792 636 2091 2130
Cuts at: 8329 9256 9690
Size: 927 434
Fragments arranged by size:
2130 2091 1073 927 842 792 651 636
434 114
MspA1I CmG'CkG
Cuts at: 0 528 811 1026 1255 1771 2573 2688 2817
Size: 528 283 215 229 516 802 115 129
Cuts at: 2817 3699 4018 4456 5688 5904 6452 6924 7283
Size: 882 319 438 1232 216 548 472 359
Cuts at: 7283 8086 8310 9690
Size: 803 224 1380
Fragments arranged by size:
1380 1232 882 803 802 548 528 516
472 438 359 319 283 229 224 216
215 129 115
NaeI GCC'GGC
Cuts at: 0 4054 6859 9583 9690
Size: 4054 2805 2724 107
Fragments arranged by size:
4054 2805 2724 107
NarI GG'CG_CC
Cuts at: 0 3049 9690
Size: 3049 6641
NcoI C'CATG_G
Cuts at: 0 590 1289 1328 3902 5507 6243 6428 9251
Size: 590 699 39 2574 1605 736 185 2823
Cuts at: 9251 9690
Size: 439
Fragments arranged by size:
2823 2574 1605 736 699 590 439 185
39
NgoMIV G'CCGG_C
Cuts at: 0 4052 6857 9581 9690
Size: 4052 2805 2724 109
Fragments arranged by size:
4052 2805 2724 109
NruI TCG'CGA
Cuts at: 0 2402 9690
Size: 2402 7288
NspI r_CATG'y
Cuts at: 0 2566 4600 4634 5974 6084 8388 9690
Size: 2566 2034 34 1340 110 2304 1302
Fragments arranged by size:
2566 2304 2034 1340 1302 110 34
PasI CC'CwG_GG
Cuts at: 0 3054 3267 4222 5121 5391 8686 8851 9132
Size: 3054 213 955 899 270 3295 165 281
Cuts at: 9132 9486 9690
Size: 354 204
Fragments arranged by size:
3295 3054 955 899 354 281 270 213
204 165
PciI A'CATG_T
Cuts at: 0 4596 9690
Size: 4596 5094
PflMI CCAn_nnn'nTGG
Cuts at: 0 1375 3786 5843 7671 8686 9436 9690
Size: 1375 2411 2057 1828 1015 750 254
Fragments arranged by size:
2411 2057 1828 1375 1015 750 254
PfoI T'CCnGG_A
Cuts at: 0 1853 4461 4911 5765 6017 6956 8720 9690
Size: 1853 2608 450 854 252 939 1764 970
Fragments arranged by size:
2608 1853 1764 970 939 854 450 252
PmlI CAC'GTG
Cuts at: 0 4206 9690
Size: 4206 5484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7042 7074 9690
Size: 7042 32 2616
Fragments arranged by size:
7042 2616 32
PpuMI rG'GwC_Cy
Cuts at: 0 2002 3085 3273 3284 5125 8015 8137 9554
Size: 2002 1083 188 11 1841 2890 122 1417
Cuts at: 9554 9690
Size: 136
Fragments arranged by size:
2890 2002 1841 1417 1083 188 136 122
11
PshAI GACnn'nnGTC
Cuts at: 0 2828 5761 6452 7180 9690
Size: 2828 2933 691 728 2510
Fragments arranged by size:
2933 2828 2510 728 691
PspOMI G'GGCC_C
Cuts at: 0 255 545 1441 1720 3057 3169 4404 5280
Size: 255 290 896 279 1337 112 1235 876
Cuts at: 5280 5394 5735 8419 8743 9425 9690
Size: 114 341 2684 324 682 265
Fragments arranged by size:
2684 1337 1235 896 876 682 341 324
290 279 265 255 114 112
PspXI vC'TCGA_Gb
Cuts at: 0 4082 9690
Size: 4082 5608
PstI C_TGCA'G
Cuts at: 0 1767 2189 2578 2996 3227 7288 8871 9690
Size: 1767 422 389 418 231 4061 1583 819
Fragments arranged by size:
4061 1767 1583 819 422 418 389 231
PvuII CAG'CTG
Cuts at: 0 811 1026 1255 1771 2688 4018 5904 6924
Size: 811 215 229 516 917 1330 1886 1020
Cuts at: 6924 8086 9690
Size: 1162 1604
Fragments arranged by size:
1886 1604 1330 1162 1020 917 811 516
229 215
SacI G_AGCT'C
Cuts at: 0 3095 4006 6041 6995 7961 9690
Size: 3095 911 2035 954 966 1729
Fragments arranged by size:
3095 2035 1729 966 954 911
SanDI GG'GwC_CC
Cuts at: 0 2002 5125 9554 9690
Size: 2002 3123 4429 136
Fragments arranged by size:
4429 3123 2002 136
SapI GCTCTTCn'nnn_
Cuts at: 0 265 789 807 992 1106 1451 1670 7001
Size: 265 524 18 185 114 345 219 5331
Cuts at: 7001 8018 9690
Size: 1017 1672
Fragments arranged by size:
5331 1672 1017 524 345 265 219 185
114 18
ScaI AGT'ACT
Cuts at: 0 727 1657 9690
Size: 727 930 8033
Fragments arranged by size:
8033 930 727
SexAI A'CCwGG_T
Cuts at: 0 3887 4560 9690
Size: 3887 673 5130
Fragments arranged by size:
5130 3887 673
SfcI C'TryA_G
Cuts at: 0 43 1247 1763 2185 2574 2992 3223 4286
Size: 43 1204 516 422 389 418 231 1063
Cuts at: 4286 7284 8867 9469 9690
Size: 2998 1583 602 221
Fragments arranged by size:
2998 1583 1204 1063 602 516 422 418
389 231 221 43
SfoI GGC'GCC
Cuts at: 0 3050 9690
Size: 3050 6640
SgrAI Cr'CCGG_yG
Cuts at: 0 7136 9690
Size: 7136 2554
SmaI CCC'GGG
Cuts at: 0 6049 6475 6889 7207 8363 9690
Size: 6049 426 414 318 1156 1327
Fragments arranged by size:
6049 1327 1156 426 414 318
SmlI C'TyrA_G
Cuts at: 0 155 661 1310 2119 4082 4123 5367 6102
Size: 155 506 649 809 1963 41 1244 735
Cuts at: 6102 6163 7217 7638 9097 9690
Size: 61 1054 421 1459 593
Fragments arranged by size:
1963 1459 1244 1054 809 735 649 593
506 421 155 61 41
SpeI A'CTAG_T
Cuts at: 0 8350 9690
Size: 8350 1340
SphI G_CATG'C
Cuts at: 0 4634 5974 9690
Size: 4634 1340 3716
Fragments arranged by size:
4634 3716 1340
SrfI GCCC'GGGC
Cuts at: 0 7207 9690
Size: 7207 2483
StuI AGG'CCT
Cuts at: 0 2200 2502 4116 6298 6964 7326 7644 9690
Size: 2200 302 1614 2182 666 362 318 2046
Fragments arranged by size:
2200 2182 2046 1614 666 362 318 302
StyI C'CwwG_G
Cuts at: 0 590 596 1177 1289 1328 1787 3902 4475
Size: 590 6 581 112 39 459 2115 573
Cuts at: 4475 5414 5507 5531 5989 6243 6428 6779 7046
Size: 939 93 24 458 254 185 351 267
Cuts at: 7046 7321 7631 7829 9138 9251 9603 9690
Size: 275 310 198 1309 113 352 87
Fragments arranged by size:
2115 1309 939 590 581 573 459 458
352 351 310 275 267 254 198 185
113 112 93 87 39 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4116 5079 5996 6103 6211 6787 7147 7833
Size: 4116 963 917 107 108 576 360 686
Cuts at: 7833 8927 9021 9690
Size: 1094 94 669
Fragments arranged by size:
4116 1094 963 917 686 669 576 360
108 107 94
TatI w'GTAC_w
Cuts at: 0 725 1655 5874 9690
Size: 725 930 4219 3816
Fragments arranged by size:
4219 3816 930 725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 403 435 462 494 3402 3434 4195 4227
Size: 403 32 27 32 2908 32 761 32
Cuts at: 4227 4323 4352 4355 4384 4433 4465 6348 6380
Size: 96 29 3 29 49 32 1883 32
Cuts at: 6380 7488 7520 9690
Size: 1108 32 2170
Fragments arranged by size:
2908 2170 1883 1108 761 403 96 49
32 32 32 32 32 32 32 29
29 27 3
Tth111I GACn'n_nGTC
Cuts at: 0 5145 5193 5235 8680 9690
Size: 5145 48 42 3445 1010
Fragments arranged by size:
5145 3445 1010 48 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 3109 4068 4104 6954 7827 8178 9690
Size: 207 2902 959 36 2850 873 351 1512
Fragments arranged by size:
2902 2850 1512 959 873 351 207 36
XhoI C'TCGA_G
Cuts at: 0 1310 4082 9097 9690
Size: 1310 2772 5015 593
Fragments arranged by size:
5015 2772 1310 593
XmaI C'CCGG_G
Cuts at: 0 6047 6473 6887 7205 8361 9690
Size: 6047 426 414 318 1156 1329
Fragments arranged by size:
6047 1329 1156 426 414 318
XmnI GAAnn'nnTTC
Cuts at: 0 582 9690
Size: 582 9108
ZraI GAC'GTC
Cuts at: 0 9568 9690
Size: 9568 122
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AseI AsiSI BamHI BclI BfrBI BmtI BsiWI
BspEI BsrGI BssHII BstZ17I ClaI DrdI EcoRI MluI
NdeI NheI NotI NsiI PacI PmeI PsiI PsrI
PvuI RsrII SacII SalI SbfI SfiI SgfI SnaBI
SspI SwaI XbaI