Restriction map for
pFN21AE9665
(Linear) (Six-base) MAPSORT of: KIEE9665.seq Check: 2480 from: 1 to: 2016
KIEE9665.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1697 1709 2016
Size: 523 1174 12 307
Fragments arranged by size:
1174 523 307 12
AfeI AGC'GCT
Cuts at: 0 1703 2016
Size: 1703 313
AleI CACnn'nnGTG
Cuts at: 0 364 394 997 1267 2016
Size: 364 30 603 270 749
Fragments arranged by size:
749 603 364 270 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1688 2016
Size: 1688 328
AlwNI CAG_nnn'CTG
Cuts at: 0 84 531 2016
Size: 84 447 1485
Fragments arranged by size:
1485 447 84
ApaLI G'TGCA_C
Cuts at: 0 1497 2016
Size: 1497 519
ApoI r'AATT_y
Cuts at: 0 206 298 778 945 1085 1309 1709 2016
Size: 206 92 480 167 140 224 400 307
Fragments arranged by size:
480 400 307 224 206 167 140 92
BamHI G'GATC_C
Cuts at: 0 1517 1538 2016
Size: 1517 21 478
Fragments arranged by size:
1517 478 21
BanI G'GyrC_C
Cuts at: 0 48 1456 2016
Size: 48 1408 560
Fragments arranged by size:
1408 560 48
BanII G_rGCy'C
Cuts at: 0 494 2016
Size: 494 1522
BbeI G_GCGC'C
Cuts at: 0 52 1460 2016
Size: 52 1408 556
Fragments arranged by size:
1408 556 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 2016
Size: 713 1303
BglI GCCn_nnn'nGGC
Cuts at: 0 415 2016
Size: 415 1601
Bme1580I G_kGCm'C
Cuts at: 0 1501 2016
Size: 1501 515
BmrI ACTGGGnnnn_n'
Cuts at: 0 1915 2016
Size: 1915 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 115 2016
Size: 52 63 1901
Fragments arranged by size:
1901 63 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 440 2016
Size: 349 91 1576
Fragments arranged by size:
1576 349 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 1008 1030 2016
Size: 1008 22 986
Fragments arranged by size:
1008 986 22
BsaHI Gr'CG_yC
Cuts at: 0 49 88 1457 2016
Size: 49 39 1369 559
Fragments arranged by size:
1369 559 49 39
BsaWI w'CCGG_w
Cuts at: 0 1399 2016
Size: 1399 617
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 499 529 1394 1424 2016
Size: 73 30 396 30 865 30 592
Fragments arranged by size:
865 592 396 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 633 652 778 996 2016
Size: 633 19 126 218 1020
Fragments arranged by size:
1020 633 218 126 19
BseYI C'CCAG_C
Cuts at: 0 65 2016
Size: 65 1951
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1260 2016
Size: 1260 756
BsiEI CG_ry'CG
Cuts at: 0 1400 2016
Size: 1400 616
BsiHKAI G_wGCw'C
Cuts at: 0 466 494 1501 2016
Size: 466 28 1007 515
Fragments arranged by size:
1007 515 466 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 95 1387 2016
Size: 95 1292 629
Fragments arranged by size:
1292 629 95
Bsp1286I G_dGCh'C
Cuts at: 0 466 494 1501 2016
Size: 466 28 1007 515
Fragments arranged by size:
1007 515 466 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1670 2016
Size: 1670 346
BsrDI GCAATG_nn'
Cuts at: 0 1187 2016
Size: 1187 829
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 2016
Size: 516 1500
BstBI TT'CG_AA
Cuts at: 0 1130 2016
Size: 1130 886
BstXI CCAn_nnnn'nTGG
Cuts at: 0 341 1017 1066 1206 2016
Size: 341 676 49 140 810
Fragments arranged by size:
810 676 341 140 49
BstYI r'GATC_y
Cuts at: 0 1517 1538 1749 2016
Size: 1517 21 211 267
Fragments arranged by size:
1517 267 211 21
BtgI C'CryG_G
Cuts at: 0 44 473 842 1353 1589 2016
Size: 44 429 369 511 236 427
Fragments arranged by size:
511 429 427 369 236 44
BtsI GCAGTG_nn'
Cuts at: 0 135 425 1393 1524 2016
Size: 135 290 968 131 492
Fragments arranged by size:
968 492 290 135 131
DraI TTT'AAA
Cuts at: 0 785 2016
Size: 785 1231
EaeI y'GGCC_r
Cuts at: 0 41 1158 2016
Size: 41 1117 858
Fragments arranged by size:
1117 858 41
EarI CTCTTCn'nnn_
Cuts at: 0 629 1751 2016
Size: 629 1122 265
Fragments arranged by size:
1122 629 265
EcoICRI GAG'CTC
Cuts at: 0 492 2016
Size: 492 1524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 115 523 1697 1709 2016
Size: 52 63 408 1174 12 307
Fragments arranged by size:
1174 408 307 63 52 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1546 2016
Size: 1546 470
EcoO109I rG'GnC_Cy
Cuts at: 0 324 2016
Size: 324 1692
EcoRI G'AATT_C
Cuts at: 0 298 945 2016
Size: 298 647 1071
Fragments arranged by size:
1071 647 298
EcoRV GAT'ATC
Cuts at: 0 1586 2016
Size: 1586 430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1572 2016
Size: 1572 444
HaeII r_GCGC'y
Cuts at: 0 52 1460 1705 2016
Size: 52 1408 245 311
Fragments arranged by size:
1408 311 245 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 1008 1040 1093 1125 2016
Size: 73 32 903 32 53 32 891
Fragments arranged by size:
903 891 73 53 32 32 32
HincII GTy'rAC
Cuts at: 0 735 2016
Size: 735 1281
KasI G'GCGC_C
Cuts at: 0 48 1456 2016
Size: 48 1408 560
Fragments arranged by size:
1408 560 48
MfeI C'AATT_G
Cuts at: 0 581 2016
Size: 581 1435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 2016
Size: 1187 829
MscI TGG'CCA
Cuts at: 0 1160 2016
Size: 1160 856
MslI CAynn'nnrTG
Cuts at: 0 339 362 364 394 997 1015 1267 1613
Size: 339 23 2 30 603 18 252 346
Cuts at: 1613 2016
Size: 403
Fragments arranged by size:
603 403 346 339 252 30 23 18
2
MspA1I CmG'CkG
Cuts at: 0 40 307 980 2016
Size: 40 267 673 1036
Fragments arranged by size:
1036 673 267 40
NarI GG'CG_CC
Cuts at: 0 49 1457 2016
Size: 49 1408 559
Fragments arranged by size:
1408 559 49
NcoI C'CATG_G
Cuts at: 0 44 473 842 1589 2016
Size: 44 429 369 747 427
Fragments arranged by size:
747 429 427 369 44
NspI r_CATG'y
Cuts at: 0 592 2016
Size: 592 1424
PasI CC'CwG_GG
Cuts at: 0 1486 2016
Size: 1486 530
PflMI CCAn_nnn'nTGG
Cuts at: 0 1167 2016
Size: 1167 849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 428 460 2016
Size: 428 32 1556
Fragments arranged by size:
1556 428 32
PpuMI rG'GwC_Cy
Cuts at: 0 324 2016
Size: 324 1692
PshAI GACnn'nnGTC
Cuts at: 0 1402 2016
Size: 1402 614
PstI C_TGCA'G
Cuts at: 0 527 1388 2016
Size: 527 861 628
Fragments arranged by size:
861 628 527
PvuII CAG'CTG
Cuts at: 0 307 2016
Size: 307 1709
SacI G_AGCT'C
Cuts at: 0 494 2016
Size: 494 1522
SapI GCTCTTCn'nnn_
Cuts at: 0 1751 2016
Size: 1751 265
SbfI CC_TGCA'GG
Cuts at: 0 527 2016
Size: 527 1489
SexAI A'CCwGG_T
Cuts at: 0 153 2016
Size: 153 1863
SfcI C'TryA_G
Cuts at: 0 523 1328 1384 2016
Size: 523 805 56 632
Fragments arranged by size:
805 632 523 56
SfoI GGC'GCC
Cuts at: 0 50 1458 2016
Size: 50 1408 558
Fragments arranged by size:
1408 558 50
SmlI C'TyrA_G
Cuts at: 0 328 455 2016
Size: 328 127 1561
Fragments arranged by size:
1561 328 127
StuI AGG'CCT
Cuts at: 0 283 2016
Size: 283 1733
StyI C'CwwG_G
Cuts at: 0 44 473 842 1460 1589 2016
Size: 44 429 369 618 129 427
Fragments arranged by size:
618 429 427 369 129 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 525 725 2016
Size: 525 200 1291
Fragments arranged by size:
1291 525 200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1394 1426 2016
Size: 1394 32 590
Fragments arranged by size:
1394 590 32
XbaI T'CTAG_A
Cuts at: 0 1752 2016
Size: 1752 264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1587 2016
Size: 1587 429
XmnI GAAnn'nnTTC
Cuts at: 0 1972 2016
Size: 1972 44
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaLI ApoI BamHI
BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstAPI BstBI
BstXI BstYI BtgI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PflMI PpiI PpuMI PshAI PstI
PvuII SacI SapI SbfI SexAI SfcI SfoI SmlI
StuI StyI TaqII TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EciI FseI FspI FspAI HindIII HpaI KpnI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XhoI XmaI ZraI