Restriction map for
pFN21AE9664
(Linear) (Six-base) MAPSORT of: KIEE9664.seq Check: 1382 from: 1 to: 1392
KIEE9664.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 233 372 1012 1028 1270 1384 1392
Size: 146 87 139 640 16 242 114 8
Fragments arranged by size:
640 242 146 139 114 87 16 8
AlwNI CAG_nnn'CTG
Cuts at: 0 76 1392
Size: 76 1316
ApoI r'AATT_y
Cuts at: 0 173 418 557 624 1014 1211 1392
Size: 173 245 139 67 390 197 181
Fragments arranged by size:
390 245 197 181 173 139 67
AseI AT'TA_AT
Cuts at: 0 487 1181 1392
Size: 487 694 211
Fragments arranged by size:
694 487 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 1009 1357 1392
Size: 1009 348 35
Fragments arranged by size:
1009 348 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 1392
Size: 594 34 764
Fragments arranged by size:
764 594 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 233 1392
Size: 233 1159
BglII A'GATC_T
Cuts at: 0 62 1392
Size: 62 1330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 725 1392
Size: 517 208 667
Fragments arranged by size:
667 517 208
BsaBI GATnn'nnATC
Cuts at: 0 107 593 1392
Size: 107 486 799
Fragments arranged by size:
799 486 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 830 860 1392
Size: 830 30 532
Fragments arranged by size:
830 532 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1392
Size: 898 494
BstYI r'GATC_y
Cuts at: 0 62 1392
Size: 62 1330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 713 748 1392
Size: 713 35 644
Fragments arranged by size:
713 644 35
EarI CTCTTCn'nnn_
Cuts at: 0 518 527 1360 1392
Size: 518 9 833 32
Fragments arranged by size:
833 518 32 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 233 372 517 725 1012 1028 1270
Size: 146 87 139 145 208 287 16 242
Cuts at: 1270 1384 1392
Size: 114 8
Fragments arranged by size:
287 242 208 146 145 139 114 87
16 8
EcoO109I rG'GnC_Cy
Cuts at: 0 903 1392
Size: 903 489
EcoRI G'AATT_C
Cuts at: 0 173 557 1211 1392
Size: 173 384 654 181
Fragments arranged by size:
654 384 181 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1392
Size: 1142 32 218
Fragments arranged by size:
1142 218 32
HincII GTy'rAC
Cuts at: 0 849 1388 1392
Size: 849 539 4
Fragments arranged by size:
849 539 4
HindIII A'AGCT_T
Cuts at: 0 790 1392
Size: 790 602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 1392
Size: 851 541
MslI CAynn'nnrTG
Cuts at: 0 1091 1392
Size: 1091 301
MspA1I CmG'CkG
Cuts at: 0 1357 1392
Size: 1357 35
NspI r_CATG'y
Cuts at: 0 401 1392
Size: 401 991
PpuMI rG'GwC_Cy
Cuts at: 0 903 1392
Size: 903 489
PsiI TTA'TAA
Cuts at: 0 761 1392
Size: 761 631
PvuII CAG'CTG
Cuts at: 0 1357 1392
Size: 1357 35
SapI GCTCTTCn'nnn_
Cuts at: 0 518 1392
Size: 518 874
SphI G_CATG'C
Cuts at: 0 401 1392
Size: 401 991
SspI AAT'ATT
Cuts at: 0 645 1265 1392
Size: 645 620 127
Fragments arranged by size:
645 620 127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 810 1392
Size: 810 582
XmnI GAAnn'nnTTC
Cuts at: 0 1265 1375 1392
Size: 1265 110 17
Fragments arranged by size:
1265 110 17
Enzymes that do cut:
AcuI AlwNI ApoI AseI BbsI BcgI BciVI BglII
BpmI BsaBI BsaXI BsgI BstYI CspCI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI MspA1I
NspI PpuMI PsiI PvuII SapI SphI SspI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI ZraI