Restriction map for
pFN21AE9660
(Linear) (Six-base) MAPSORT of: KIEE9660.seq Check: 6951 from: 1 to: 741
KIEE9660.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 741
Size: 485 256
AflII C'TTAA_G
Cuts at: 0 697 741
Size: 697 44
AflIII A'CryG_T
Cuts at: 0 639 741
Size: 639 102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 741
Size: 139 602
AseI AT'TA_AT
Cuts at: 0 415 741
Size: 415 326
BamHI G'GATC_C
Cuts at: 0 687 741
Size: 687 54
BanI G'GyrC_C
Cuts at: 0 88 741
Size: 88 653
BanII G_rGCy'C
Cuts at: 0 38 741
Size: 38 703
BbeI G_GCGC'C
Cuts at: 0 92 741
Size: 92 649
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 741
Size: 254 487
BglI GCCn_nnn'nGGC
Cuts at: 0 65 97 741
Size: 65 32 644
Fragments arranged by size:
644 65 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 367 741
Size: 188 179 374
Fragments arranged by size:
374 188 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 741
Size: 739 2
BsaBI GATnn'nnATC
Cuts at: 0 586 741
Size: 586 155
BsaHI Gr'CG_yC
Cuts at: 0 26 89 741
Size: 26 63 652
Fragments arranged by size:
652 63 26
BsaWI w'CCGG_w
Cuts at: 0 46 741
Size: 46 695
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 118 741
Size: 19 99 623
Fragments arranged by size:
623 99 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 314 741
Size: 123 191 427
Fragments arranged by size:
427 191 123
BsiHKAI G_wGCw'C
Cuts at: 0 144 377 741
Size: 144 233 364
Fragments arranged by size:
364 233 144
Bsp1286I G_dGCh'C
Cuts at: 0 38 144 377 741
Size: 38 106 233 364
Fragments arranged by size:
364 233 106 38
BspEI T'CCGG_A
Cuts at: 0 46 741
Size: 46 695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 741
Size: 69 672
BsrFI r'CCGG_y
Cuts at: 0 85 741
Size: 85 656
BstYI r'GATC_y
Cuts at: 0 687 741
Size: 687 54
Bsu36I CC'TnA_GG
Cuts at: 0 130 741
Size: 130 611
BtgI C'CryG_G
Cuts at: 0 38 741
Size: 38 703
EarI CTCTTCn'nnn_
Cuts at: 0 263 472 741
Size: 263 209 269
Fragments arranged by size:
269 263 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 367 485 741
Size: 188 179 118 256
Fragments arranged by size:
256 188 179 118
HaeII r_GCGC'y
Cuts at: 0 92 741
Size: 92 649
HincII GTy'rAC
Cuts at: 0 732 741
Size: 732 9
HindIII A'AGCT_T
Cuts at: 0 298 497 511 741
Size: 298 199 14 230
Fragments arranged by size:
298 230 199 14
KasI G'GCGC_C
Cuts at: 0 88 741
Size: 88 653
MfeI C'AATT_G
Cuts at: 0 675 741
Size: 675 66
MspA1I CmG'CkG
Cuts at: 0 40 741
Size: 40 701
NaeI GCC'GGC
Cuts at: 0 87 741
Size: 87 654
NarI GG'CG_CC
Cuts at: 0 89 741
Size: 89 652
NgoMIV G'CCGG_C
Cuts at: 0 85 741
Size: 85 656
NspI r_CATG'y
Cuts at: 0 643 741
Size: 643 98
PciI A'CATG_T
Cuts at: 0 639 741
Size: 639 102
PstI C_TGCA'G
Cuts at: 0 716 741
Size: 716 25
SacII CC_GC'GG
Cuts at: 0 41 741
Size: 41 700
SfcI C'TryA_G
Cuts at: 0 604 712 741
Size: 604 108 29
Fragments arranged by size:
604 108 29
SfoI GGC'GCC
Cuts at: 0 90 741
Size: 90 651
SgrAI Cr'CCGG_yG
Cuts at: 0 85 741
Size: 85 656
SmlI C'TyrA_G
Cuts at: 0 697 718 741
Size: 697 21 23
Fragments arranged by size:
697 23 21
XmnI GAAnn'nnTTC
Cuts at: 0 707 741
Size: 707 34
Enzymes that do cut:
AcuI AflII AflIII AlfI AseI BamHI BanI BanII
BbeI BbsI BglI BpmI BpuEI BsaBI BsaHI BsaWI
BseRI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstYI
Bsu36I BtgI EarI Eco57MI HaeII HincII HindIII KasI
MfeI MspA1I NaeI NarI NgoMIV NspI PciI PstI
SacII SfcI SfoI SgrAI SmlI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HpaI
KpnI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI