Restriction map for
pFN21AE9659
(Linear) (Six-base) MAPSORT of: KIEE9659.seq Check: 2869 from: 1 to: 666
KIEE9659.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 666
Size: 370 296
AflII C'TTAA_G
Cuts at: 0 646 666
Size: 646 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 666
Size: 27 639
AlwNI CAG_nnn'CTG
Cuts at: 0 501 666
Size: 501 165
ApaI G_GGCC'C
Cuts at: 0 63 666
Size: 63 603
BanI G'GyrC_C
Cuts at: 0 17 331 666
Size: 17 314 335
Fragments arranged by size:
335 314 17
BanII G_rGCy'C
Cuts at: 0 63 562 666
Size: 63 499 104
Fragments arranged by size:
499 104 63
BbeI G_GCGC'C
Cuts at: 0 21 666
Size: 21 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 666
Size: 31 635
BbvCI CC'TCA_GC
Cuts at: 0 606 666
Size: 606 60
Bme1580I G_kGCm'C
Cuts at: 0 63 334 666
Size: 63 271 332
Fragments arranged by size:
332 271 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 563 666
Size: 563 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 666
Size: 655 11
Bpu10I CC'TnA_GC
Cuts at: 0 606 666
Size: 606 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 262 666
Size: 247 15 404
Fragments arranged by size:
404 247 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 666
Size: 103 563
BsaHI Gr'CG_yC
Cuts at: 0 18 28 666
Size: 18 10 638
Fragments arranged by size:
638 18 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 433 487 666
Size: 49 384 54 179
Fragments arranged by size:
384 179 54 49
BseYI C'CCAG_C
Cuts at: 0 510 666
Size: 510 156
Bsp1286I G_dGCh'C
Cuts at: 0 63 334 562 666
Size: 63 271 228 104
Fragments arranged by size:
271 228 104 63
BspHI T'CATG_A
Cuts at: 0 374 666
Size: 374 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 666
Size: 662 4
BsrBI CCG'CTC
Cuts at: 0 390 666
Size: 390 276
BsrDI GCAATG_nn'
Cuts at: 0 128 666
Size: 128 538
BstXI CCAn_nnnn'nTGG
Cuts at: 0 327 666
Size: 327 339
BstYI r'GATC_y
Cuts at: 0 266 362 666
Size: 266 96 304
Fragments arranged by size:
304 266 96
BtgI C'CryG_G
Cuts at: 0 326 449 666
Size: 326 123 217
Fragments arranged by size:
326 217 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 279 666
Size: 279 387
EaeI y'GGCC_r
Cuts at: 0 149 666
Size: 149 517
EarI CTCTTCn'nnn_
Cuts at: 0 464 666
Size: 464 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 655 666
Size: 370 285 11
Fragments arranged by size:
370 285 11
EcoO109I rG'GnC_Cy
Cuts at: 0 59 60 666
Size: 59 1 606
Fragments arranged by size:
606 59 1
FspI TGC'GCA
Cuts at: 0 202 220 666
Size: 202 18 446
Fragments arranged by size:
446 202 18
FspAI rTGC'GCAy
Cuts at: 0 202 666
Size: 202 464
HaeII r_GCGC'y
Cuts at: 0 21 213 666
Size: 21 192 453
Fragments arranged by size:
453 192 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 520 552 666
Size: 520 32 114
Fragments arranged by size:
520 114 32
KasI G'GCGC_C
Cuts at: 0 17 666
Size: 17 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 666
Size: 308 358
MscI TGG'CCA
Cuts at: 0 151 666
Size: 151 515
MslI CAynn'nnrTG
Cuts at: 0 409 666
Size: 409 257
MspA1I CmG'CkG
Cuts at: 0 348 666
Size: 348 318
NarI GG'CG_CC
Cuts at: 0 18 666
Size: 18 648
NcoI C'CATG_G
Cuts at: 0 326 449 666
Size: 326 123 217
Fragments arranged by size:
326 217 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 666
Size: 501 165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 666
Size: 532 32 102
Fragments arranged by size:
532 102 32
PspOMI G'GGCC_C
Cuts at: 0 59 666
Size: 59 607
PvuII CAG'CTG
Cuts at: 0 348 666
Size: 348 318
SfoI GGC'GCC
Cuts at: 0 19 666
Size: 19 647
SmlI C'TyrA_G
Cuts at: 0 262 277 646 666
Size: 262 15 369 20
Fragments arranged by size:
369 262 20 15
StyI C'CwwG_G
Cuts at: 0 320 326 449 666
Size: 320 6 123 217
Fragments arranged by size:
320 217 123 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 666
Size: 442 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 291 666
Size: 81 210 375
Fragments arranged by size:
375 210 81
Enzymes that do cut:
AcuI AflII AloI AlwNI ApaI BanI BanII BbeI
BbsI BbvCI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI Bsp1286I BspHI BspMI BsrBI BsrDI
BstXI BstYI BtgI DrdI EaeI EarI Eco57MI EcoO109I
FspI FspAI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NarI NcoI PflMI PpiI PspOMI PvuII SfoI
SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI