(Linear) (Six-base) MAPSORT of: KIEE9657.seq  Check: 3236  from: 1  to: 507



KIEE9657.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:42  ..

BanI G'GyrC_C

Cuts at:      0     240     507 
   Size:        240     267
 
BanII G_rGCy'C

Cuts at:      0     431     507 
   Size:        431      76
 
BmtI G_CTAG'C

Cuts at:      0     136     507 
   Size:        136     371
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     507 
   Size:        139     368
 
BseYI C'CCAG_C

Cuts at:      0      21     507 
   Size:         21     486
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     507 
   Size:        191     316
 
BsmI GAATG_Cn'

Cuts at:      0     486     507 
   Size:        486      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     431     507 
   Size:        431      76
 
BspHI T'CATG_A

Cuts at:      0     221     507 
   Size:        221     286
 
BsrDI GCAATG_nn'

Cuts at:      0     354     507 
   Size:        354     153
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     142     507 
   Size:        142     365
 
EarI CTCTTCn'nnn_

Cuts at:      0     202     507 
   Size:        202     305
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     507 
   Size:        139     368
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     399     507 
   Size:        399     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     203     235     507 
   Size:        203      32     272

  Fragments arranged by size: 

                 272     203      32
 
MfeI C'AATT_G

Cuts at:      0     359     507 
   Size:        359     148
 
MslI CAynn'nnrTG

Cuts at:      0     231     507 
   Size:        231     276
 
NheI G'CTAG_C

Cuts at:      0     132     507 
   Size:        132     375
 
NspI r_CATG'y

Cuts at:      0     337     507 
   Size:        337     170
 
PstI C_TGCA'G

Cuts at:      0     452     507 
   Size:        452      55
 
SfcI C'TryA_G

Cuts at:      0     164     180     369     448     507 
   Size:        164      16     189      79      59

  Fragments arranged by size: 

                 189     164      79      59      16
 
SspI AAT'ATT

Cuts at:      0      45     507 
   Size:         45     462
 

 Enzymes that do cut:

     BanI    BanII     BmtI     BpmI    BseYI     BsgI     BsmI Bsp1286I
    BspHI    BsrDI   BstAPI     EarI  Eco57MI     FalI    Hin4I     MfeI
     MslI     NheI     NspI     PstI     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI