(Linear) (Six-base) MAPSORT of: KIEE9655.seq  Check: 7667  from: 1  to: 384



KIEE9655.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 19:03  ..

AflIII A'CryG_T

Cuts at:      0     135     384 
   Size:        135     249
 
AhdI GACnn_n'nnGTC

Cuts at:      0     125     384 
   Size:        125     259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     382     384 
   Size:        382       2
 
BfrBI ATG'CAT

Cuts at:      0     217     384 
   Size:        217     167
 
BmgBI CAC'GTC

Cuts at:      0     136     384 
   Size:        136     248
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     384 
   Size:         79     305
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     384 
   Size:        196     188
 
BsaWI w'CCGG_w

Cuts at:      0      28     384 
   Size:         28     356
 
BspEI T'CCGG_A

Cuts at:      0      28     384 
   Size:         28     356
 
BsrBI CCG'CTC

Cuts at:      0      59     384 
   Size:         59     325
 
BsrFI r'CCGG_y

Cuts at:      0      70     384 
   Size:         70     314
 
BsrGI T'GTAC_A

Cuts at:      0     158     384 
   Size:        158     226
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     214     384 
   Size:        214     170
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     384 
   Size:        196     188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     143     384 
   Size:        111      32     241

  Fragments arranged by size: 

                 241     111      32
 
MfeI C'AATT_G

Cuts at:      0     311     384 
   Size:        311      73
 
MslI CAynn'nnrTG

Cuts at:      0     134     384 
   Size:        134     250
 
MspA1I CmG'CkG

Cuts at:      0     154     384 
   Size:        154     230
 
NaeI GCC'GGC

Cuts at:      0      72     384 
   Size:         72     312
 
NgoMIV G'CCGG_C

Cuts at:      0      70     384 
   Size:         70     314
 
NsiI A_TGCA'T

Cuts at:      0     219     384 
   Size:        219     165
 
PfoI T'CCnGG_A

Cuts at:      0     173     384 
   Size:        173     211
 
TatI w'GTAC_w

Cuts at:      0     158     384 
   Size:        158     226
 

 Enzymes that do cut:

   AflIII     AhdI     BbsI    BfrBI    BmgBI     BmrI     BpmI    BsaWI
    BspEI    BsrBI    BsrFI    BsrGI   BstAPI  Eco57MI    Hin4I     MfeI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     PfoI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI