Restriction map for
pFN21AE9641
(Linear) (Six-base) MAPSORT of: KIEE9641.seq Check: 3280 from: 1 to: 1188
KIEE9641.orf
With 182 enzymes: SgfI *
March 22, 2012 18:36 ..
AatII G_ACGT'C
Cuts at: 0 862 1188
Size: 862 326
AccI GT'mk_AC
Cuts at: 0 338 1016 1188
Size: 338 678 172
Fragments arranged by size:
678 338 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 706 1188
Size: 194 512 482
Fragments arranged by size:
512 482 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 1188
Size: 221 967
AlwNI CAG_nnn'CTG
Cuts at: 0 684 769 1188
Size: 684 85 419
Fragments arranged by size:
684 419 85
BglII A'GATC_T
Cuts at: 0 135 1188
Size: 135 1053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1056 1188
Size: 902 154 132
Fragments arranged by size:
902 154 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1070 1143 1188
Size: 274 796 73 45
Fragments arranged by size:
796 274 73 45
BsaAI yAC'GTr
Cuts at: 0 984 1188
Size: 984 204
BsaHI Gr'CG_yC
Cuts at: 0 859 1188
Size: 859 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 1054 1084 1188
Size: 112 30 912 30 104
Fragments arranged by size:
912 112 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 834 967 983 1105 1188
Size: 598 236 133 16 122 83
Fragments arranged by size:
598 236 133 122 83 16
BseYI C'CCAG_C
Cuts at: 0 342 1188
Size: 342 846
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1188
Size: 191 997
BsmI GAATG_Cn'
Cuts at: 0 491 1188
Size: 491 697
BspHI T'CATG_A
Cuts at: 0 1077 1188
Size: 1077 111
BsrGI T'GTAC_A
Cuts at: 0 108 1170 1188
Size: 108 1062 18
Fragments arranged by size:
1062 108 18
BstYI r'GATC_y
Cuts at: 0 135 1188
Size: 135 1053
BtgI C'CryG_G
Cuts at: 0 1025 1188
Size: 1025 163
BtsI GCAGTG_nn'
Cuts at: 0 1038 1106 1188
Size: 1038 68 82
Fragments arranged by size:
1038 82 68
DrdI GACnn_nn'nnGTC
Cuts at: 0 864 1188
Size: 864 324
EarI CTCTTCn'nnn_
Cuts at: 0 23 1188
Size: 23 1165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 706 902 1056 1188
Size: 194 512 196 154 132
Fragments arranged by size:
512 196 194 154 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 34 1188
Size: 34 1154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 1054 1086 1188
Size: 127 32 895 32 102
Fragments arranged by size:
895 127 102 32 32
HindIII A'AGCT_T
Cuts at: 0 113 1188
Size: 113 1075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1188
Size: 965 223
MspA1I CmG'CkG
Cuts at: 0 522 684 1188
Size: 522 162 504
Fragments arranged by size:
522 504 162
NdeI CA'TA_TG
Cuts at: 0 105 1188
Size: 105 1083
PasI CC'CwG_GG
Cuts at: 0 528 1188
Size: 528 660
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 835 925 1188
Size: 705 130 90 263
Fragments arranged by size:
705 263 130 90
PfoI T'CCnGG_A
Cuts at: 0 494 1188
Size: 494 694
PshAI GACnn'nnGTC
Cuts at: 0 809 1073 1188
Size: 809 264 115
Fragments arranged by size:
809 264 115
PstI C_TGCA'G
Cuts at: 0 677 811 1188
Size: 677 134 377
Fragments arranged by size:
677 377 134
PvuII CAG'CTG
Cuts at: 0 522 684 1188
Size: 522 162 504
Fragments arranged by size:
522 504 162
SfcI C'TryA_G
Cuts at: 0 673 761 776 807 1188
Size: 673 88 15 31 381
Fragments arranged by size:
673 381 88 31 15
SmlI C'TyrA_G
Cuts at: 0 289 1085 1158 1188
Size: 289 796 73 30
Fragments arranged by size:
796 289 73 30
SnaBI TAC'GTA
Cuts at: 0 984 1188
Size: 984 204
StuI AGG'CCT
Cuts at: 0 736 1188
Size: 736 452
StyI C'CwwG_G
Cuts at: 0 245 1188
Size: 245 943
TatI w'GTAC_w
Cuts at: 0 108 128 268 1170 1188
Size: 108 20 140 902 18
Fragments arranged by size:
902 140 108 20 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 859 1144 1188
Size: 859 285 44
Fragments arranged by size:
859 285 44
XmnI GAAnn'nnTTC
Cuts at: 0 17 616 1188
Size: 17 599 572
Fragments arranged by size:
599 572 17
ZraI GAC'GTC
Cuts at: 0 860 1188
Size: 860 328
Enzymes that do cut:
AatII AccI AcuI AlfI AlwNI BglII BpmI BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsmI BspHI
BsrGI BstYI BtgI BtsI DrdI EarI Eco57MI FalI
Hin4I HindIII MmeI MspA1I NdeI PasI PflMI PfoI
PshAI PstI PvuII SfcI SmlI SnaBI StuI StyI
TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI