Restriction map for
pFN21AE9640
(Linear) (Six-base) MAPSORT of: KIEE9640.seq Check: 276 from: 1 to: 5715
KIEE9640.orf
With 182 enzymes: SgfI *
March 22, 2012 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3525 4662 5715
Size: 3525 1137 1053
Fragments arranged by size:
3525 1137 1053
AclI AA'CG_TT
Cuts at: 0 4116 5061 5715
Size: 4116 945 654
Fragments arranged by size:
4116 945 654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1492 2632 3794 4070 4679 4984 5715
Size: 504 988 1140 1162 276 609 305 731
Fragments arranged by size:
1162 1140 988 731 609 504 305 276
AflII C'TTAA_G
Cuts at: 0 3022 3163 5715
Size: 3022 141 2552
Fragments arranged by size:
3022 2552 141
AflIII A'CryG_T
Cuts at: 0 473 1459 2378 2837 4509 5396 5715
Size: 473 986 919 459 1672 887 319
Fragments arranged by size:
1672 986 919 887 473 459 319
AgeI A'CCGG_T
Cuts at: 0 3494 5715
Size: 3494 2221
AhdI GACnn_n'nnGTC
Cuts at: 0 4053 4065 5715
Size: 4053 12 1650
Fragments arranged by size:
4053 1650 12
AleI CACnn'nnGTG
Cuts at: 0 1531 3535 5715
Size: 1531 2004 2180
Fragments arranged by size:
2180 2004 1531
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2863 2895 5715
Size: 2863 32 2820
Fragments arranged by size:
2863 2820 32
AlwNI CAG_nnn'CTG
Cuts at: 0 441 709 1552 4741 5481 5623 5715
Size: 441 268 843 3189 740 142 92
Fragments arranged by size:
3189 843 740 441 268 142 92
ApoI r'AATT_y
Cuts at: 0 1327 1789 2509 2955 3637 5317 5609 5715
Size: 1327 462 720 446 682 1680 292 106
Fragments arranged by size:
1680 1327 720 682 462 446 292 106
AvaI C'yCGr_G
Cuts at: 0 179 903 1192 1834 2740 2975 3356 4427
Size: 179 724 289 642 906 235 381 1071
Cuts at: 4427 5715
Size: 1288
Fragments arranged by size:
1288 1071 906 724 642 381 289 235
179
BamHI G'GATC_C
Cuts at: 0 1170 3906 5715
Size: 1170 2736 1809
Fragments arranged by size:
2736 1809 1170
BanI G'GyrC_C
Cuts at: 0 741 3935 5676 5715
Size: 741 3194 1741 39
Fragments arranged by size:
3194 1741 741 39
BanII G_rGCy'C
Cuts at: 0 19 2205 2373 2569 2917 3459 3592 3814
Size: 19 2186 168 196 348 542 133 222
Cuts at: 3814 4883 5715
Size: 1069 832
Fragments arranged by size:
2186 1069 832 542 348 222 196 168
133 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 452 467 2259 2456 3782 4109 4958 5155
Size: 452 15 1792 197 1326 327 849 197
Cuts at: 5155 5411 5715
Size: 256 304
Fragments arranged by size:
1792 1326 849 452 327 304 256 197
197 15
BbvCI CC'TCA_GC
Cuts at: 0 4544 5447 5715
Size: 4544 903 268
Fragments arranged by size:
4544 903 268
BclI T'GATC_A
Cuts at: 0 1901 2540 5715
Size: 1901 639 3175
Fragments arranged by size:
3175 1901 639
BglI GCCn_nnn'nGGC
Cuts at: 0 1112 1599 2033 5715
Size: 1112 487 434 3682
Fragments arranged by size:
3682 1112 487 434
BglII A'GATC_T
Cuts at: 0 3960 4929 5159 5715
Size: 3960 969 230 556
Fragments arranged by size:
3960 969 556 230
BlpI GC'TnA_GC
Cuts at: 0 434 2911 3329 5715
Size: 434 2477 418 2386
Fragments arranged by size:
2477 2386 434 418
Bme1580I G_kGCm'C
Cuts at: 0 2212 3111 5679 5715
Size: 2212 899 2568 36
Fragments arranged by size:
2568 2212 899 36
BmgBI CAC'GTC
Cuts at: 0 1890 5715
Size: 1890 3825
BmrI ACTGGGnnnn_n'
Cuts at: 0 459 2355 2690 3290 3822 3927 4133 4296
Size: 459 1896 335 600 532 105 206 163
Cuts at: 4296 5611 5715
Size: 1315 104
Fragments arranged by size:
1896 1315 600 532 459 335 206 163
105 104
BmtI G_CTAG'C
Cuts at: 0 5194 5715
Size: 5194 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2342 5121 5715
Size: 2342 2779 594
Fragments arranged by size:
2779 2342 594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1529 1687 2063 2357 2644 4545 4598 5715
Size: 1529 158 376 294 287 1901 53 1117
Fragments arranged by size:
1901 1529 1117 376 294 287 158 53
Bpu10I CC'TnA_GC
Cuts at: 0 2204 4544 5447 5715
Size: 2204 2340 903 268
Fragments arranged by size:
2340 2204 903 268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 2029 3139 5715
Size: 136 1893 1110 2576
Fragments arranged by size:
2576 1893 1110 136
BsaI GGTCTCn'nnnn_
Cuts at: 0 1289 4310 4399 5005 5715
Size: 1289 3021 89 606 710
Fragments arranged by size:
3021 1289 710 606 89
BsaAI yAC'GTr
Cuts at: 0 474 1460 5715
Size: 474 986 4255
Fragments arranged by size:
4255 986 474
BsaBI GATnn'nnATC
Cuts at: 0 1495 1553 1846 5715
Size: 1495 58 293 3869
Fragments arranged by size:
3869 1495 293 58
BsaHI Gr'CG_yC
Cuts at: 0 4 5715
Size: 4 5711
BsaWI w'CCGG_w
Cuts at: 0 3461 3494 5715
Size: 3461 33 2221
Fragments arranged by size:
3461 2221 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 126 156 1552 1582 2341 2371 2863 2893
Size: 126 30 1396 30 759 30 492 30
Cuts at: 2893 4903 4933 5715
Size: 2010 30 782
Fragments arranged by size:
2010 1396 782 759 492 126 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 64 274 277 394 397 400 403
Size: 61 3 210 3 117 3 3 3
Cuts at: 403 499 1060 1063 1285 1321 2788 3535 3695
Size: 96 561 3 222 36 1467 747 160
Cuts at: 3695 3698 3880 4172 4837 5715
Size: 3 182 292 665 878
Fragments arranged by size:
1467 878 747 665 561 292 222 210
182 160 117 96 61 36 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 222 870 2826 3361 3585 4571 5592 5705
Size: 222 648 1956 535 224 986 1021 113
Cuts at: 5705 5715
Size: 10
Fragments arranged by size:
1956 1021 986 648 535 224 222 113
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1504 1657 2140 4625 5512 5715
Size: 1504 153 483 2485 887 203
Fragments arranged by size:
2485 1504 887 483 203 153
BsiHKAI G_wGCw'C
Cuts at: 0 3330 3459 3814 5715
Size: 3330 129 355 1901
Fragments arranged by size:
3330 1901 355 129
Bsp1286I G_dGCh'C
Cuts at: 0 19 2205 2212 2373 2569 2917 3111 3330
Size: 19 2186 7 161 196 348 194 219
Cuts at: 3330 3459 3592 3814 4883 5679 5715
Size: 129 133 222 1069 796 36
Fragments arranged by size:
2186 1069 796 348 222 219 196 194
161 133 129 36 19 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3227 3525 4328 4662 5688 5715
Size: 3227 298 803 334 1026 27
Fragments arranged by size:
3227 1026 803 334 298 27
BsrBI CCG'CTC
Cuts at: 0 1599 2352 5127 5715
Size: 1599 753 2775 588
Fragments arranged by size:
2775 1599 753 588
BsrDI GCAATG_nn'
Cuts at: 0 542 2786 3071 4174 5075 5600 5715
Size: 542 2244 285 1103 901 525 115
Fragments arranged by size:
2244 1103 901 542 525 285 115
BsrFI r'CCGG_y
Cuts at: 0 3494 5715
Size: 3494 2221
BsrGI T'GTAC_A
Cuts at: 0 2253 4759 5715
Size: 2253 2506 956
Fragments arranged by size:
2506 2253 956
BssSI C'ACGA_G
Cuts at: 0 812 5715
Size: 812 4903
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2823 2841 2971 3150 3325 5715
Size: 2823 18 130 179 175 2390
Fragments arranged by size:
2823 2390 179 175 130 18
BstEII G'GTnAC_C
Cuts at: 0 4580 5715
Size: 4580 1135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2553 5715
Size: 2553 3162
BstYI r'GATC_y
Cuts at: 0 898 1170 1274 1424 1490 1958 3018 3601
Size: 898 272 104 150 66 468 1060 583
Cuts at: 3601 3906 3960 4929 5159 5715
Size: 305 54 969 230 556
Fragments arranged by size:
1060 969 898 583 556 468 305 272
230 150 104 66 54
Bsu36I CC'TnA_GG
Cuts at: 0 4283 4814 5715
Size: 4283 531 901
Fragments arranged by size:
4283 901 531
BtgI C'CryG_G
Cuts at: 0 5208 5289 5423 5715
Size: 5208 81 134 292
Fragments arranged by size:
5208 292 134 81
BtsI GCAGTG_nn'
Cuts at: 0 48 1215 1398 1638 3111 3386 3717 5715
Size: 48 1167 183 240 1473 275 331 1998
Fragments arranged by size:
1998 1473 1167 331 275 240 183 48
ClaI AT'CG_AT
Cuts at: 0 3266 5715
Size: 3266 2449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 696 731 1332 1367 5715
Size: 696 35 601 35 4348
Fragments arranged by size:
4348 696 601 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1872 3325 5715
Size: 1872 1453 2390
Fragments arranged by size:
2390 1872 1453
DrdI GACnn_nn'nnGTC
Cuts at: 0 2383 3510 5715
Size: 2383 1127 2205
Fragments arranged by size:
2383 2205 1127
EaeI y'GGCC_r
Cuts at: 0 1591 3383 5715
Size: 1591 1792 2332
Fragments arranged by size:
2332 1792 1591
EarI CTCTTCn'nnn_
Cuts at: 0 101 113 263 352 1277 1298 1748 2702
Size: 101 12 150 89 925 21 450 954
Cuts at: 2702 3055 3857 4694 4955 4973 5117 5715
Size: 353 802 837 261 18 144 598
Fragments arranged by size:
954 925 837 802 598 450 353 261
150 144 101 89 21 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5479 5715
Size: 5479 236
EcoICRI GAG'CTC
Cuts at: 0 3457 3812 5715
Size: 3457 355 1903
Fragments arranged by size:
3457 1903 355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1492 1529 1687 2063 2357 2632 2644
Size: 504 988 37 158 376 294 275 12
Cuts at: 2644 3794 4070 4545 4598 4679 4984 5715
Size: 1150 276 475 53 81 305 731
Fragments arranged by size:
1150 988 731 504 475 376 305 294
276 275 158 81 53 37 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1282 1303 2284 3166 3223 5485 5715
Size: 1282 21 981 882 57 2262 230
Fragments arranged by size:
2262 1282 981 882 230 57 21
EcoO109I rG'GnC_Cy
Cuts at: 0 111 183 778 1782 2175 2305 3366 4149
Size: 111 72 595 1004 393 130 1061 783
Cuts at: 4149 4767 5022 5715
Size: 618 255 693
Fragments arranged by size:
1061 1004 783 693 618 595 393 255
130 111 72
EcoRI G'AATT_C
Cuts at: 0 1327 5609 5715
Size: 1327 4282 106
Fragments arranged by size:
4282 1327 106
EcoRV GAT'ATC
Cuts at: 0 3630 5715
Size: 3630 2085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4267 5715
Size: 4267 1448
HaeII r_GCGC'y
Cuts at: 0 210 2324 4524 5715
Size: 210 2114 2200 1191
Fragments arranged by size:
2200 2114 1191 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 409 441 916 948 4039 4051
Size: 112 32 265 32 475 32 3091 12
Cuts at: 4051 4071 4083 4714 4746 4903 4935 5151 5183
Size: 20 12 631 32 157 32 216 32
Cuts at: 5183 5715
Size: 532
Fragments arranged by size:
3091 631 532 475 265 216 157 112
32 32 32 32 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 4008 5715
Size: 4008 1707
HindIII A'AGCT_T
Cuts at: 0 334 2056 5715
Size: 334 1722 3659
Fragments arranged by size:
3659 1722 334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2048 2125 4082 5715
Size: 2048 77 1957 1633
Fragments arranged by size:
2048 1957 1633 77
MscI TGG'CCA
Cuts at: 0 3385 5715
Size: 3385 2330
MslI CAynn'nnrTG
Cuts at: 0 1531 2551 3535 3725 3941 4379 4514 5213
Size: 1531 1020 984 190 216 438 135 699
Cuts at: 5213 5715
Size: 502
Fragments arranged by size:
1531 1020 984 699 502 438 216 190
135
MspA1I CmG'CkG
Cuts at: 0 661 676 1665 1704 5373 5547 5715
Size: 661 15 989 39 3669 174 168
Fragments arranged by size:
3669 989 661 174 168 39 15
NcoI C'CATG_G
Cuts at: 0 5208 5423 5715
Size: 5208 215 292
Fragments arranged by size:
5208 292 215
NdeI CA'TA_TG
Cuts at: 0 1322 4353 5715
Size: 1322 3031 1362
Fragments arranged by size:
3031 1362 1322
NheI G'CTAG_C
Cuts at: 0 5190 5715
Size: 5190 525
NspI r_CATG'y
Cuts at: 0 1629 2382 2826 2841 2877 3096 3291 4453
Size: 1629 753 444 15 36 219 195 1162
Cuts at: 4453 4513 4533 5400 5715
Size: 60 20 867 315
Fragments arranged by size:
1629 1162 867 753 444 315 219 195
60 36 20 15
PciI A'CATG_T
Cuts at: 0 2378 2837 4509 5396 5715
Size: 2378 459 1672 887 319
Fragments arranged by size:
2378 1672 887 459 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 1323 1732 1872 2757 3834 5715
Size: 1323 409 140 885 1077 1881
Fragments arranged by size:
1881 1323 1077 885 409 140
PfoI T'CCnGG_A
Cuts at: 0 440 1277 1961 5715
Size: 440 837 684 3754
Fragments arranged by size:
3754 837 684 440
PmlI CAC'GTG
Cuts at: 0 474 5715
Size: 474 5241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1588 1620 2422 2454 2863 2895 4327 4359
Size: 1588 32 802 32 409 32 1432 32
Cuts at: 4359 5715
Size: 1356
Fragments arranged by size:
1588 1432 1356 802 409 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 111 183 778 1782 5022 5715
Size: 111 72 595 1004 3240 693
Fragments arranged by size:
3240 1004 693 595 111 72
PshAI GACnn'nnGTC
Cuts at: 0 2263 5715
Size: 2263 3452
PsiI TTA'TAA
Cuts at: 0 3251 5203 5715
Size: 3251 1952 512
Fragments arranged by size:
3251 1952 512
PspXI vC'TCGA_Gb
Cuts at: 0 903 3356 5715
Size: 903 2453 2359
Fragments arranged by size:
2453 2359 903
PstI C_TGCA'G
Cuts at: 0 1664 3146 5715
Size: 1664 1482 2569
Fragments arranged by size:
2569 1664 1482
PvuII CAG'CTG
Cuts at: 0 661 1665 5373 5547 5715
Size: 661 1004 3708 174 168
Fragments arranged by size:
3708 1004 661 174 168
SacI G_AGCT'C
Cuts at: 0 3459 3814 5715
Size: 3459 355 1901
Fragments arranged by size:
3459 1901 355
SanDI GG'GwC_CC
Cuts at: 0 183 5715
Size: 183 5532
SapI GCTCTTCn'nnn_
Cuts at: 0 352 5117 5715
Size: 352 4765 598
Fragments arranged by size:
4765 598 352
ScaI AGT'ACT
Cuts at: 0 4217 5715
Size: 4217 1498
SexAI A'CCwGG_T
Cuts at: 0 2990 5715
Size: 2990 2725
SfcI C'TryA_G
Cuts at: 0 1660 2141 3142 4733 5715
Size: 1660 481 1001 1591 982
Fragments arranged by size:
1660 1591 1001 982 481
SmlI C'TyrA_G
Cuts at: 0 151 903 2044 3022 3154 3163 3356 5715
Size: 151 752 1141 978 132 9 193 2359
Fragments arranged by size:
2359 1141 978 752 193 151 132 9
SphI G_CATG'C
Cuts at: 0 4533 5715
Size: 4533 1182
SspI AAT'ATT
Cuts at: 0 3805 4209 5052 5715
Size: 3805 404 843 663
Fragments arranged by size:
3805 843 663 404
StuI AGG'CCT
Cuts at: 0 517 2076 2479 3445 4436 5715
Size: 517 1559 403 966 991 1279
Fragments arranged by size:
1559 1279 991 966 517 403
StyI C'CwwG_G
Cuts at: 0 764 800 1580 2241 2792 3369 5018 5208
Size: 764 36 780 661 551 577 1649 190
Cuts at: 5208 5423 5486 5715
Size: 215 63 229
Fragments arranged by size:
1649 780 764 661 577 551 229 215
190 63 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 583 2389 4049 5715
Size: 110 473 1806 1660 1666
Fragments arranged by size:
1806 1666 1660 473 110
TatI w'GTAC_w
Cuts at: 0 2253 2939 3183 4215 4759 5715
Size: 2253 686 244 1032 544 956
Fragments arranged by size:
2253 1032 956 686 544 244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 5493 5715
Size: 582 4911 222
Fragments arranged by size:
4911 582 222
XhoI C'TCGA_G
Cuts at: 0 903 3356 5715
Size: 903 2453 2359
Fragments arranged by size:
2453 2359 903
XmnI GAAnn'nnTTC
Cuts at: 0 2437 4342 5715
Size: 2437 1905 1373
Fragments arranged by size:
2437 1905 1373
Enzymes that do cut:
AarI AclI AcuI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NdeI NheI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuII SacI SanDI SapI ScaI SexAI SfcI
SmlI SphI SspI StuI StyI TaqII TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AlfI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI
BstZ17I BtgZI DraI EagI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PmeI PspOMI PsrI PvuI
RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI
XmaI ZraI