Restriction map for
pFN21AE9638
(Linear) (Six-base) MAPSORT of: KIEE9638.seq Check: 9465 from: 1 to: 5484
KIEE9638.orf
With 182 enzymes: SgfI *
March 22, 2012 18:36 ..
AclI AA'CG_TT
Cuts at: 0 686 5484
Size: 686 4798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1091 1094 1402 1930 2155 2333 2615
Size: 95 996 3 308 528 225 178 282
Cuts at: 2615 3529 3740 4878 5484
Size: 914 211 1138 606
Fragments arranged by size:
1138 996 914 606 528 308 282 225
211 178 95 3
AflIII A'CryG_T
Cuts at: 0 5078 5484
Size: 5078 406
AhdI GACnn_n'nnGTC
Cuts at: 0 3267 5484
Size: 3267 2217
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4918 4950 5484
Size: 4918 32 534
Fragments arranged by size:
4918 534 32
AlwNI CAG_nnn'CTG
Cuts at: 0 73 1104 2508 2547 5484
Size: 73 1031 1404 39 2937
Fragments arranged by size:
2937 1404 1031 73 39
ApoI r'AATT_y
Cuts at: 0 1306 1950 2005 2917 2990 3197 3215 3884
Size: 1306 644 55 912 73 207 18 669
Cuts at: 3884 4624 5188 5484
Size: 740 564 296
Fragments arranged by size:
1306 912 740 669 644 564 296 207
73 55 18
AvaI C'yCGr_G
Cuts at: 0 2696 3935 4031 5484
Size: 2696 1239 96 1453
Fragments arranged by size:
2696 1453 1239 96
AvrII C'CTAG_G
Cuts at: 0 1536 4096 5484
Size: 1536 2560 1388
Fragments arranged by size:
2560 1536 1388
BanI G'GyrC_C
Cuts at: 0 487 1215 1665 4982 5153 5484
Size: 487 728 450 3317 171 331
Fragments arranged by size:
3317 728 487 450 331 171
BanII G_rGCy'C
Cuts at: 0 409 1232 1967 4478 5484
Size: 409 823 735 2511 1006
Fragments arranged by size:
2511 1006 823 735 409
BbsI GAAGACnn'nnnn_
Cuts at: 0 540 592 1165 2033 2056 3323 5484
Size: 540 52 573 868 23 1267 2161
Fragments arranged by size:
2161 1267 868 573 540 52 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 683 3348 3382 5484
Size: 649 34 2665 34 2102
Fragments arranged by size:
2665 2102 649 34 34
BclI T'GATC_A
Cuts at: 0 5235 5484
Size: 5235 249
BfrBI ATG'CAT
Cuts at: 0 55 4848 5484
Size: 55 4793 636
Fragments arranged by size:
4793 636 55
BglII A'GATC_T
Cuts at: 0 3504 5484
Size: 3504 1980
BlpI GC'TnA_GC
Cuts at: 0 3479 5484
Size: 3479 2005
Bme1580I G_kGCm'C
Cuts at: 0 560 1668 4058 5484
Size: 560 1108 2390 1426
Fragments arranged by size:
2390 1426 1108 560
BmgBI CAC'GTC
Cuts at: 0 4713 5484
Size: 4713 771
BmrI ACTGGGnnnn_n'
Cuts at: 0 820 1839 3135 3849 4727 5317 5484
Size: 820 1019 1296 714 878 590 167
Fragments arranged by size:
1296 1019 878 820 714 590 167
BmtI G_CTAG'C
Cuts at: 0 1486 5484
Size: 1486 3998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 738 5484
Size: 738 4746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 851 3478 3994 4605 4740 5484
Size: 767 84 2627 516 611 135 744
Fragments arranged by size:
2627 767 744 611 516 135 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4664 5484
Size: 4664 820
BsaI GGTCTCn'nnnn_
Cuts at: 0 1383 3445 4523 4682 5114 5423 5484
Size: 1383 2062 1078 159 432 309 61
Fragments arranged by size:
2062 1383 1078 432 309 159 61
BsaAI yAC'GTr
Cuts at: 0 3472 5484
Size: 3472 2012
BsaBI GATnn'nnATC
Cuts at: 0 4385 5484
Size: 4385 1099
BsaHI Gr'CG_yC
Cuts at: 0 3354 5484
Size: 3354 2130
BsaWI w'CCGG_w
Cuts at: 0 2093 3637 4532 5056 5484
Size: 2093 1544 895 524 428
Fragments arranged by size:
2093 1544 895 524 428
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 740 770 3632 3662 5484
Size: 740 30 2862 30 1822
Fragments arranged by size:
2862 1822 740 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 2779 2782 3181 3375 3694 4084 4602
Size: 43 2736 3 399 194 319 390 518
Cuts at: 4602 4705 4925 5190 5375 5484
Size: 103 220 265 185 109
Fragments arranged by size:
2736 518 399 390 319 265 220 194
185 109 103 43 3
BseYI C'CCAG_C
Cuts at: 0 224 1277 1815 3810 5484
Size: 224 1053 538 1995 1674
Fragments arranged by size:
1995 1674 1053 538 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1963 3746 5484
Size: 515 1448 1783 1738
Fragments arranged by size:
1783 1738 1448 515
BsiHKAI G_wGCw'C
Cuts at: 0 1967 4574 5484
Size: 1967 2607 910
Fragments arranged by size:
2607 1967 910
BsmI GAATG_Cn'
Cuts at: 0 1375 4101 5484
Size: 1375 2726 1383
Fragments arranged by size:
2726 1383 1375
Bsp1286I G_dGCh'C
Cuts at: 0 409 560 1232 1668 1967 4058 4478 4574
Size: 409 151 672 436 299 2091 420 96
Cuts at: 4574 5484
Size: 910
Fragments arranged by size:
2091 910 672 436 420 409 299 151
96
BspEI T'CCGG_A
Cuts at: 0 2093 3637 5056 5484
Size: 2093 1544 1419 428
Fragments arranged by size:
2093 1544 1419 428
BspHI T'CATG_A
Cuts at: 0 5160 5484
Size: 5160 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 1428 2715 5484
Size: 202 1226 1287 2769
Fragments arranged by size:
2769 1287 1226 202
BsrBI CCG'CTC
Cuts at: 0 676 3177 3940 5288 5484
Size: 676 2501 763 1348 196
Fragments arranged by size:
2501 1348 763 676 196
BsrDI GCAATG_nn'
Cuts at: 0 57 3557 5335 5484
Size: 57 3500 1778 149
Fragments arranged by size:
3500 1778 149 57
BsrFI r'CCGG_y
Cuts at: 0 1212 3891 5484
Size: 1212 2679 1593
Fragments arranged by size:
2679 1593 1212
BssSI C'ACGA_G
Cuts at: 0 566 3379 5484
Size: 566 2813 2105
Fragments arranged by size:
2813 2105 566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2291 3475 5484
Size: 2291 1184 2009
Fragments arranged by size:
2291 2009 1184
BstEII G'GTnAC_C
Cuts at: 0 1696 5484
Size: 1696 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1597 5023 5484
Size: 1597 3426 461
Fragments arranged by size:
3426 1597 461
BstYI r'GATC_y
Cuts at: 0 1790 2931 3289 3404 3504 5179 5355 5484
Size: 1790 1141 358 115 100 1675 176 129
Fragments arranged by size:
1790 1675 1141 358 176 129 115 100
Bsu36I CC'TnA_GG
Cuts at: 0 2969 3914 4749 5484
Size: 2969 945 835 735
Fragments arranged by size:
2969 945 835 735
BtgI C'CryG_G
Cuts at: 0 2031 4833 4966 5259 5484
Size: 2031 2802 133 293 225
Fragments arranged by size:
2802 2031 293 225 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 341 5484
Size: 341 5143
BtsI GCAGTG_nn'
Cuts at: 0 2289 2373 5118 5484
Size: 2289 84 2745 366
Fragments arranged by size:
2745 2289 366 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4926 4961 5484
Size: 4926 35 523
Fragments arranged by size:
4926 523 35
DraI TTT'AAA
Cuts at: 0 1409 5484
Size: 1409 4075
DrdI GACnn_nn'nnGTC
Cuts at: 0 2644 5484
Size: 2644 2840
EaeI y'GGCC_r
Cuts at: 0 2838 5262 5484
Size: 2838 2424 222
Fragments arranged by size:
2838 2424 222
EarI CTCTTCn'nnn_
Cuts at: 0 371 411 666 1949 2891 2941 3092 3101
Size: 371 40 255 1283 942 50 151 9
Cuts at: 3101 3167 3560 3671 3998 4115 4682 5484
Size: 66 393 111 327 117 567 802
Fragments arranged by size:
1283 942 802 567 393 371 327 255
151 117 111 66 50 40 9
EcoICRI GAG'CTC
Cuts at: 0 1965 5484
Size: 1965 3519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 767 851 1091 1094 1402 1930 2155
Size: 95 672 84 240 3 308 528 225
Cuts at: 2155 2333 2615 3478 3529 3740 3994 4605 4740
Size: 178 282 863 51 211 254 611 135
Cuts at: 4740 4878 5484
Size: 138 606
Fragments arranged by size:
863 672 611 606 528 308 282 254
240 225 211 178 138 135 95 84
51 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3382 5484
Size: 3382 2102
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1377 1937 3375 4327 5269 5484
Size: 999 378 560 1438 952 942 215
Fragments arranged by size:
1438 999 952 942 560 378 215
EcoRI G'AATT_C
Cuts at: 0 1950 3215 5484
Size: 1950 1265 2269
Fragments arranged by size:
2269 1950 1265
EcoRV GAT'ATC
Cuts at: 0 2463 3635 4775 5484
Size: 2463 1172 1140 709
Fragments arranged by size:
2463 1172 1140 709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4908 5484
Size: 4908 576
HaeII r_GCGC'y
Cuts at: 0 3516 5484
Size: 3516 1968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 190 204 222 1894 1926 1954 1986
Size: 172 18 14 18 1672 32 28 32
Cuts at: 1986 2417 2449 3241 3253 3273 3285 4075 4099
Size: 431 32 792 12 20 12 790 24
Cuts at: 4099 4107 4131 4282 4314 5347 5379 5383 5401
Size: 8 24 151 32 1033 32 4 18
Cuts at: 5401 5415 5433 5484
Size: 14 18 51
Fragments arranged by size:
1672 1033 792 790 431 172 151 51
32 32 32 32 32 28 24 24
20 18 18 18 18 14 14 12
12 8 4
HincII GTy'rAC
Cuts at: 0 2399 3029 5484
Size: 2399 630 2455
Fragments arranged by size:
2455 2399 630
HindIII A'AGCT_T
Cuts at: 0 80 5484
Size: 80 5404
MfeI C'AATT_G
Cuts at: 0 1089 5484
Size: 1089 4395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 170 1186 1937 2438 2897 3646 3693
Size: 89 81 1016 751 501 459 749 47
Cuts at: 3693 5247 5484
Size: 1554 237
Fragments arranged by size:
1554 1016 751 749 501 459 237 89
81 47
MscI TGG'CCA
Cuts at: 0 5264 5484
Size: 5264 220
MslI CAynn'nnrTG
Cuts at: 0 50 739 2558 3770 3830 4991 5021 5042
Size: 50 689 1819 1212 60 1161 30 21
Cuts at: 5042 5159 5484
Size: 117 325
Fragments arranged by size:
1819 1212 1161 689 325 117 60 50
30 21
MspA1I CmG'CkG
Cuts at: 0 432 1593 2635 3539 3573 4494 5484
Size: 432 1161 1042 904 34 921 990
Fragments arranged by size:
1161 1042 990 921 904 432 34
NaeI GCC'GGC
Cuts at: 0 1214 5484
Size: 1214 4270
NcoI C'CATG_G
Cuts at: 0 2031 4833 4966 5259 5484
Size: 2031 2802 133 293 225
Fragments arranged by size:
2802 2031 293 225 133
NdeI CA'TA_TG
Cuts at: 0 1785 4411 5039 5484
Size: 1785 2626 628 445
Fragments arranged by size:
2626 1785 628 445
NgoMIV G'CCGG_C
Cuts at: 0 1212 5484
Size: 1212 4272
NheI G'CTAG_C
Cuts at: 0 1482 5484
Size: 1482 4002
NruI TCG'CGA
Cuts at: 0 360 5484
Size: 360 5124
NsiI A_TGCA'T
Cuts at: 0 57 4850 5484
Size: 57 4793 634
Fragments arranged by size:
4793 634 57
NspI r_CATG'y
Cuts at: 0 5082 5484
Size: 5082 402
PacI TTA_AT'TAA
Cuts at: 0 3851 5484
Size: 3851 1633
PciI A'CATG_T
Cuts at: 0 5078 5484
Size: 5078 406
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 2508 5484
Size: 139 2369 2976
Fragments arranged by size:
2976 2369 139
PfoI T'CCnGG_A
Cuts at: 0 132 2849 5484
Size: 132 2717 2635
Fragments arranged by size:
2717 2635 132
PmlI CAC'GTG
Cuts at: 0 3472 5484
Size: 3472 2012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 2132 2164 5484
Size: 151 32 1949 32 3320
Fragments arranged by size:
3320 1949 151 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1937 3375 4327 5269 5484
Size: 1937 1438 952 942 215
Fragments arranged by size:
1937 1438 952 942 215
PshAI GACnn'nnGTC
Cuts at: 0 5229 5484
Size: 5229 255
PsiI TTA'TAA
Cuts at: 0 1777 3428 5462 5484
Size: 1777 1651 2034 22
Fragments arranged by size:
2034 1777 1651 22
PstI C_TGCA'G
Cuts at: 0 5349 5484
Size: 5349 135
PvuII CAG'CTG
Cuts at: 0 432 1593 3539 3573 4494 5484
Size: 432 1161 1946 34 921 990
Fragments arranged by size:
1946 1161 990 921 432 34
SacI G_AGCT'C
Cuts at: 0 1967 5484
Size: 1967 3517
SanDI GG'GwC_CC
Cuts at: 0 1937 5269 5484
Size: 1937 3332 215
Fragments arranged by size:
3332 1937 215
SapI GCTCTTCn'nnn_
Cuts at: 0 666 2891 3092 3101 3167 3560 5484
Size: 666 2225 201 9 66 393 1924
Fragments arranged by size:
2225 1924 666 393 201 66 9
SfcI C'TryA_G
Cuts at: 0 3928 5345 5484
Size: 3928 1417 139
Fragments arranged by size:
3928 1417 139
SmlI C'TyrA_G
Cuts at: 0 4679 5484
Size: 4679 805
SspI AAT'ATT
Cuts at: 0 369 1084 2259 2733 5484
Size: 369 715 1175 474 2751
Fragments arranged by size:
2751 1175 715 474 369
StuI AGG'CCT
Cuts at: 0 4747 5094 5196 5484
Size: 4747 347 102 288
Fragments arranged by size:
4747 347 288 102
StyI C'CwwG_G
Cuts at: 0 1536 2031 4096 4833 4966 5259 5484
Size: 1536 495 2065 737 133 293 225
Fragments arranged by size:
2065 1536 737 495 293 225 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3508 4871 5132 5484
Size: 3508 1363 261 352
Fragments arranged by size:
3508 1363 352 261
TatI w'GTAC_w
Cuts at: 0 5105 5484
Size: 5105 379
Tth111I GACn'n_nGTC
Cuts at: 0 2641 5484
Size: 2641 2843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2509 2793 5403 5462 5484
Size: 2509 284 2610 59 22
Fragments arranged by size:
2610 2509 284 59 22
XmnI GAAnn'nnTTC
Cuts at: 0 1736 2144 5484
Size: 1736 408 3340
Fragments arranged by size:
3340 1736 408
Enzymes that do cut:
AclI AcuI AflIII AhdI AloI AlwNI ApoI AvaI
AvrII BanI BanII BbsI BcgI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PacI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PstI PvuII SacI SanDI SapI SfcI SmlI
SspI StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BciVI BglI Bpu10I BsiEI BsiWI BsmBI
BsrGI BssHII BstBI BstZ17I ClaI DraIII EagI EciI
FseI FspI FspAI HpaI KasI KpnI MluI NarI
NotI PasI PmeI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI
XbaI XhoI XmaI ZraI