Restriction map for
pFN21AE9634
(Linear) (Six-base) MAPSORT of: KIEE9634.seq Check: 2071 from: 1 to: 4122
KIEE9634.orf
With 182 enzymes: SgfI *
January 14, 2011 16:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1517 2152 3778 4122
Size: 1517 635 1626 344
Fragments arranged by size:
1626 1517 635 344
AfeI AGC'GCT
Cuts at: 0 3680 4122
Size: 3680 442
AflII C'TTAA_G
Cuts at: 0 1222 4122
Size: 1222 2900
AflIII A'CryG_T
Cuts at: 0 16 4122
Size: 16 4106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2213 2223 4122
Size: 2213 10 1899
Fragments arranged by size:
2213 1899 10
AlwNI CAG_nnn'CTG
Cuts at: 0 397 1475 1495 2451 4122
Size: 397 1078 20 956 1671
Fragments arranged by size:
1671 1078 956 397 20
ApaI G_GGCC'C
Cuts at: 0 1035 3874 4122
Size: 1035 2839 248
Fragments arranged by size:
2839 1035 248
ApoI r'AATT_y
Cuts at: 0 1339 1914 2712 2742 2762 2905 2929 3439
Size: 1339 575 798 30 20 143 24 510
Cuts at: 3439 4122
Size: 683
Fragments arranged by size:
1339 798 683 575 510 143 30 24
20
AvaI C'yCGr_G
Cuts at: 0 39 56 1174 2877 4122
Size: 39 17 1118 1703 1245
Fragments arranged by size:
1703 1245 1118 39 17
AvrII C'CTAG_G
Cuts at: 0 3417 3444 4122
Size: 3417 27 678
Fragments arranged by size:
3417 678 27
BanI G'GyrC_C
Cuts at: 0 323 402 695 2139 4122
Size: 323 79 293 1444 1983
Fragments arranged by size:
1983 1444 323 293 79
BanII G_rGCy'C
Cuts at: 0 1035 1089 1175 3874 4122
Size: 1035 54 86 2699 248
Fragments arranged by size:
2699 1035 248 86 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 783 2015 4115 4122
Size: 783 1232 2100 7
Fragments arranged by size:
2100 1232 783 7
BbvCI CC'TCA_GC
Cuts at: 0 87 131 4122
Size: 87 44 3991
Fragments arranged by size:
3991 87 44
BciVI GTATCCnnnnn_n'
Cuts at: 0 2321 4122
Size: 2321 1801
BclI T'GATC_A
Cuts at: 0 1785 2730 4122
Size: 1785 945 1392
Fragments arranged by size:
1785 1392 945
BfrBI ATG'CAT
Cuts at: 0 2631 4122
Size: 2631 1491
BglII A'GATC_T
Cuts at: 0 796 937 4122
Size: 796 141 3185
Fragments arranged by size:
3185 796 141
Bme1580I G_kGCm'C
Cuts at: 0 174 405 1035 3874 4122
Size: 174 231 630 2839 248
Fragments arranged by size:
2839 630 248 231 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 328 385 533 575 641 4122
Size: 328 57 148 42 66 3481
Fragments arranged by size:
3481 328 148 66 57 42
BmtI G_CTAG'C
Cuts at: 0 3037 4122
Size: 3037 1085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 455 1063 1251 1679 2203 3190 3287
Size: 182 273 608 188 428 524 987 97
Cuts at: 3287 4122
Size: 835
Fragments arranged by size:
987 835 608 524 428 273 188 182
97
Bpu10I CC'TnA_GC
Cuts at: 0 87 131 4122
Size: 87 44 3991
Fragments arranged by size:
3991 87 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 1174 2351 4122
Size: 1174 1177 1771
Fragments arranged by size:
1771 1177 1174
BsaBI GATnn'nnATC
Cuts at: 0 900 2839 4122
Size: 900 1939 1283
Fragments arranged by size:
1939 1283 900
BsaWI w'CCGG_w
Cuts at: 0 1721 3213 4122
Size: 1721 1492 909
Fragments arranged by size:
1721 1492 909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 939 969 1249 1279 2170 2200 4122
Size: 939 30 280 30 891 30 1922
Fragments arranged by size:
1922 939 891 280 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 1192 1216 1360 1363 2161 2164 2191
Size: 80 1112 24 144 3 798 3 27
Cuts at: 2191 3589 4122
Size: 1398 533
Fragments arranged by size:
1398 1112 798 533 144 80 27 24
3 3
BseYI C'CCAG_C
Cuts at: 0 3579 3788 4122
Size: 3579 209 334
Fragments arranged by size:
3579 334 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2145 2321 4122
Size: 2145 176 1801
Fragments arranged by size:
2145 1801 176
BsiHKAI G_wGCw'C
Cuts at: 0 1089 4122
Size: 1089 3033
BsmI GAATG_Cn'
Cuts at: 0 2212 3379 4122
Size: 2212 1167 743
Fragments arranged by size:
2212 1167 743
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2496 4122
Size: 2496 1626
Bsp1286I G_dGCh'C
Cuts at: 0 174 405 1035 1089 1175 3874 4122
Size: 174 231 630 54 86 2699 248
Fragments arranged by size:
2699 630 248 231 174 86 54
BspEI T'CCGG_A
Cuts at: 0 3213 4122
Size: 3213 909
BspHI T'CATG_A
Cuts at: 0 431 1554 3507 3719 4122
Size: 431 1123 1953 212 403
Fragments arranged by size:
1953 1123 431 403 212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 984 3227 4122
Size: 477 507 2243 895
Fragments arranged by size:
2243 895 507 477
BsrDI GCAATG_nn'
Cuts at: 0 2585 3122 3980 4122
Size: 2585 537 858 142
Fragments arranged by size:
2585 858 537 142
BsrFI r'CCGG_y
Cuts at: 0 788 4122
Size: 788 3334
BsrGI T'GTAC_A
Cuts at: 0 3683 3848 4122
Size: 3683 165 274
Fragments arranged by size:
3683 274 165
BssSI C'ACGA_G
Cuts at: 0 1789 4122
Size: 1789 2333
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3979 4122
Size: 3979 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1132 3648 4122
Size: 1132 2516 474
Fragments arranged by size:
2516 1132 474
BstYI r'GATC_y
Cuts at: 0 796 937 4122
Size: 796 141 3185
Fragments arranged by size:
3185 796 141
Bsu36I CC'TnA_GG
Cuts at: 0 2144 4122
Size: 2144 1978
BtgI C'CryG_G
Cuts at: 0 1277 1393 4122
Size: 1277 116 2729
Fragments arranged by size:
2729 1277 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1042 4122
Size: 1042 3080
BtsI GCAGTG_nn'
Cuts at: 0 3805 4122
Size: 3805 317
ClaI AT'CG_AT
Cuts at: 0 2261 3057 4122
Size: 2261 796 1065
Fragments arranged by size:
2261 1065 796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2359 2394 4122
Size: 2359 35 1728
Fragments arranged by size:
2359 1728 35
DraI TTT'AAA
Cuts at: 0 2711 4122
Size: 2711 1411
DrdI GACnn_nn'nnGTC
Cuts at: 0 50 4122
Size: 50 4072
EaeI y'GGCC_r
Cuts at: 0 2684 2856 3119 4122
Size: 2684 172 263 1003
Fragments arranged by size:
2684 1003 263 172
EarI CTCTTCn'nnn_
Cuts at: 0 281 1075 1658 1892 2377 4122
Size: 281 794 583 234 485 1745
Fragments arranged by size:
1745 794 583 485 281 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3667 4122
Size: 3667 455
EcoICRI GAG'CTC
Cuts at: 0 1087 4122
Size: 1087 3035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 455 1063 1251 1517 1679 2152 2203
Size: 182 273 608 188 266 162 473 51
Cuts at: 2203 3190 3287 3778 4122
Size: 987 97 491 344
Fragments arranged by size:
987 608 491 473 344 273 266 188
182 162 97 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 413 4122
Size: 413 3709
EcoO109I rG'GnC_Cy
Cuts at: 0 104 271 659 914 1032 1996 4122
Size: 104 167 388 255 118 964 2126
Fragments arranged by size:
2126 964 388 255 167 118 104
EcoRI G'AATT_C
Cuts at: 0 2742 2762 2905 3439 4122
Size: 2742 20 143 534 683
Fragments arranged by size:
2742 683 534 143 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 191 292 1234 1237 4122
Size: 191 101 942 3 2885
Fragments arranged by size:
2885 942 191 101 3
HaeII r_GCGC'y
Cuts at: 0 3634 3682 4122
Size: 3634 48 440
Fragments arranged by size:
3634 440 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 643 675 1038 1070 1441 1473
Size: 426 32 185 32 363 32 371 32
Cuts at: 1473 3185 3217 3769 3801 3999 4031 4122
Size: 1712 32 552 32 198 32 91
Fragments arranged by size:
1712 552 426 371 363 198 185 91
32 32 32 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 3123 3338 4122
Size: 3123 215 784
Fragments arranged by size:
3123 784 215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1287 2882 3282 4122
Size: 1093 194 1595 400 840
Fragments arranged by size:
1595 1093 840 400 194
MscI TGG'CCA
Cuts at: 0 2686 2858 3121 4122
Size: 2686 172 263 1001
Fragments arranged by size:
2686 1001 263 172
MslI CAynn'nnrTG
Cuts at: 0 683 710 2806 4122
Size: 683 27 2096 1316
Fragments arranged by size:
2096 1316 683 27
MspA1I CmG'CkG
Cuts at: 0 2682 4122
Size: 2682 1440
NcoI C'CATG_G
Cuts at: 0 1277 4122
Size: 1277 2845
NheI G'CTAG_C
Cuts at: 0 3033 4122
Size: 3033 1089
NsiI A_TGCA'T
Cuts at: 0 2633 4122
Size: 2633 1489
NspI r_CATG'y
Cuts at: 0 20 1828 2578 2805 2811 3115 3588 4122
Size: 20 1808 750 227 6 304 473 534
Fragments arranged by size:
1808 750 534 473 304 227 20 6
PasI CC'CwG_GG
Cuts at: 0 397 2233 4122
Size: 397 1836 1889
Fragments arranged by size:
1889 1836 397
PciI A'CATG_T
Cuts at: 0 16 4122
Size: 16 4106
PflMI CCAn_nnn'nTGG
Cuts at: 0 397 4122
Size: 397 3725
PfoI T'CCnGG_A
Cuts at: 0 408 1656 4122
Size: 408 1248 2466
Fragments arranged by size:
2466 1248 408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 810 842 1138 1170 4122
Size: 810 32 296 32 2952
Fragments arranged by size:
2952 810 296 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 659 914 1996 4122
Size: 659 255 1082 2126
Fragments arranged by size:
2126 1082 659 255
PshAI GACnn'nnGTC
Cuts at: 0 2941 4122
Size: 2941 1181
PspOMI G'GGCC_C
Cuts at: 0 1031 3870 4122
Size: 1031 2839 252
Fragments arranged by size:
2839 1031 252
PspXI vC'TCGA_Gb
Cuts at: 0 2877 4122
Size: 2877 1245
PstI C_TGCA'G
Cuts at: 0 3863 4122
Size: 3863 259
PvuII CAG'CTG
Cuts at: 0 2682 4122
Size: 2682 1440
SacI G_AGCT'C
Cuts at: 0 1089 4122
Size: 1089 3033
ScaI AGT'ACT
Cuts at: 0 3481 4122
Size: 3481 641
SfcI C'TryA_G
Cuts at: 0 1298 3859 4122
Size: 1298 2561 263
Fragments arranged by size:
2561 1298 263
SmaI CCC'GGG
Cuts at: 0 41 4122
Size: 41 4081
SmlI C'TyrA_G
Cuts at: 0 1222 2877 4122
Size: 1222 1655 1245
Fragments arranged by size:
1655 1245 1222
SphI G_CATG'C
Cuts at: 0 2578 3588 4122
Size: 2578 1010 534
Fragments arranged by size:
2578 1010 534
SspI AAT'ATT
Cuts at: 0 1842 4122
Size: 1842 2280
StuI AGG'CCT
Cuts at: 0 3624 4122
Size: 3624 498
StyI C'CwwG_G
Cuts at: 0 1277 2532 2853 3417 3444 3563 3647 4122
Size: 1277 1255 321 564 27 119 84 475
Fragments arranged by size:
1277 1255 564 475 321 119 84 27
SwaI ATTT'AAAT
Cuts at: 0 2711 4122
Size: 2711 1411
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 722 824 1562 2902 3266 4122
Size: 264 458 102 738 1340 364 856
Fragments arranged by size:
1340 856 738 458 364 264 102
TatI w'GTAC_w
Cuts at: 0 2111 2951 3479 3683 3848 4040 4122
Size: 2111 840 528 204 165 192 82
Fragments arranged by size:
2111 840 528 204 192 165 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2069 2101 2170 2202 4122
Size: 2069 32 69 32 1920
Fragments arranged by size:
2069 1920 69 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 1284 1591 3462 3624 4122
Size: 1275 9 307 1871 162 498
Fragments arranged by size:
1871 1275 498 307 162 9
XhoI C'TCGA_G
Cuts at: 0 2877 4122
Size: 2877 1245
XmaI C'CCGG_G
Cuts at: 0 39 4122
Size: 39 4083
XmnI GAAnn'nnTTC
Cuts at: 0 1217 3707 3751 4024 4122
Size: 1217 2490 44 273 98
Fragments arranged by size:
2490 1217 273 98 44
Enzymes that do cut:
AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI
MmeI MscI MslI MspA1I NcoI NheI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI ScaI SfcI SmaI SmlI
SphI SspI StuI StyI SwaI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BcgI BglI BlpI BmgBI BplI BpuEI BsaAI BsaHI
BsiEI BsiWI BsrBI BssHII BstBI BstEII BstZ17I DraIII
EagI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI
NruI PacI PmeI PmlI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI Tth111I XbaI ZraI