Restriction map for
pFN21AE9633
(Linear) (Six-base) MAPSORT of: KIEE9633.seq Check: 7177 from: 1 to: 4008
KIEE9633.orf
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1398 4008
Size: 1398 2610
AatII G_ACGT'C
Cuts at: 0 383 4008
Size: 383 3625
AccI GT'mk_AC
Cuts at: 0 578 947 959 1463 4008
Size: 578 369 12 504 2545
Fragments arranged by size:
2545 578 504 369 12
Acc65I G'GTAC_C
Cuts at: 0 752 4008
Size: 752 3256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 640 919 1561 1675 2973 3204 4008
Size: 186 454 279 642 114 1298 231 804
Fragments arranged by size:
1298 804 642 454 279 231 186 114
AfeI AGC'GCT
Cuts at: 0 1129 4008
Size: 1129 2879
AflII C'TTAA_G
Cuts at: 0 2098 4008
Size: 2098 1910
AflIII A'CryG_T
Cuts at: 0 602 1109 4008
Size: 602 507 2899
Fragments arranged by size:
2899 602 507
AhdI GACnn_n'nnGTC
Cuts at: 0 3376 4008
Size: 3376 632
AleI CACnn'nnGTG
Cuts at: 0 1978 4008
Size: 1978 2030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2303 4008
Size: 2303 1705
AlwNI CAG_nnn'CTG
Cuts at: 0 618 2433 2528 3272 3740 3855 4008
Size: 618 1815 95 744 468 115 153
Fragments arranged by size:
1815 744 618 468 153 115 95
ApaI G_GGCC'C
Cuts at: 0 1771 1915 2779 3041 4008
Size: 1771 144 864 262 967
Fragments arranged by size:
1771 967 864 262 144
ApaLI G'TGCA_C
Cuts at: 0 250 2392 4008
Size: 250 2142 1616
Fragments arranged by size:
2142 1616 250
ApoI r'AATT_y
Cuts at: 0 106 1057 4008
Size: 106 951 2951
Fragments arranged by size:
2951 951 106
AvaI C'yCGr_G
Cuts at: 0 487 1179 1369 1603 1915 2677 2692 2961
Size: 487 692 190 234 312 762 15 269
Cuts at: 2961 3014 3797 4008
Size: 53 783 211
Fragments arranged by size:
783 762 692 487 312 269 234 211
190 53 15
BamHI G'GATC_C
Cuts at: 0 935 4008
Size: 935 3073
BanI G'GyrC_C
Cuts at: 0 752 1302 1823 2316 2352 2697 3007 3026
Size: 752 550 521 493 36 345 310 19
Cuts at: 3026 3787 4008
Size: 761 221
Fragments arranged by size:
761 752 550 521 493 345 310 221
36 19
BanII G_rGCy'C
Cuts at: 0 1771 1818 1915 2779 2861 2951 3002 3041
Size: 1771 47 97 864 82 90 51 39
Cuts at: 3041 3452 4008
Size: 411 556
Fragments arranged by size:
1771 864 556 411 97 90 82 51
47 39
BbeI G_GCGC'C
Cuts at: 0 1827 2320 2701 4008
Size: 1827 493 381 1307
Fragments arranged by size:
1827 1307 493 381
BbsI GAAGACnn'nnnn_
Cuts at: 0 1729 2018 3210 4008
Size: 1729 289 1192 798
Fragments arranged by size:
1729 1192 798 289
BbvCI CC'TCA_GC
Cuts at: 0 2521 3229 3391 4008
Size: 2521 708 162 617
Fragments arranged by size:
2521 708 617 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2334 2368 4008
Size: 2334 34 1640
Fragments arranged by size:
2334 1640 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3115 4008
Size: 3115 893
BclI T'GATC_A
Cuts at: 0 1245 3846 4008
Size: 1245 2601 162
Fragments arranged by size:
2601 1245 162
BglI GCCn_nnn'nGGC
Cuts at: 0 2658 2955 3662 4008
Size: 2658 297 707 346
Fragments arranged by size:
2658 707 346 297
BlpI GC'TnA_GC
Cuts at: 0 2836 2848 3421 4008
Size: 2836 12 573 587
Fragments arranged by size:
2836 587 573 12
Bme1580I G_kGCm'C
Cuts at: 0 254 445 1007 1305 1771 1915 2396 2779
Size: 254 191 562 298 466 144 481 383
Cuts at: 2779 3041 4008
Size: 262 967
Fragments arranged by size:
967 562 481 466 383 298 262 254
191 144
BmgBI CAC'GTC
Cuts at: 0 174 417 2391 3189 3750 4008
Size: 174 243 1974 798 561 258
Fragments arranged by size:
1974 798 561 258 243 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 456 600 1703 4008
Size: 349 107 144 1103 2305
Fragments arranged by size:
2305 1103 349 144 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 317 319 2522 4008
Size: 317 2 2203 1486
Fragments arranged by size:
2203 1486 317 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 568 1120 1243 1654 2718 3492 4008
Size: 229 339 552 123 411 1064 774 516
Fragments arranged by size:
1064 774 552 516 411 339 229 123
Bpu10I CC'TnA_GC
Cuts at: 0 2521 3229 3391 4008
Size: 2521 708 162 617
Fragments arranged by size:
2521 708 617 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2227 4008
Size: 2227 1781
BsaI GGTCTCn'nnnn_
Cuts at: 0 860 1509 3495 4008
Size: 860 649 1986 513
Fragments arranged by size:
1986 860 649 513
BsaAI yAC'GTr
Cuts at: 0 288 1110 4008
Size: 288 822 2898
Fragments arranged by size:
2898 822 288
BsaHI Gr'CG_yC
Cuts at: 0 380 484 1824 2317 2698 4008
Size: 380 104 1340 493 381 1310
Fragments arranged by size:
1340 1310 493 381 380 104
BsaWI w'CCGG_w
Cuts at: 0 928 1799 3524 3635 4008
Size: 928 871 1725 111 373
Fragments arranged by size:
1725 928 871 373 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 1473 1503 2292 2322 2526 2556
Size: 857 30 586 30 789 30 204 30
Cuts at: 2556 2716 2746 4008
Size: 160 30 1262
Fragments arranged by size:
1262 857 789 586 204 160 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 226 838 1246 1249 1276 1279 1282
Size: 193 33 612 408 3 27 3 3
Cuts at: 1282 1294 2041 2313 2326 2472 2797 2959 4008
Size: 12 747 272 13 146 325 162 1049
Fragments arranged by size:
1049 747 612 408 325 272 193 162
146 33 27 13 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 494 611 1124 1189 1764 2147 2755 3033
Size: 494 117 513 65 575 383 608 278
Cuts at: 3033 3040 4008
Size: 7 968
Fragments arranged by size:
968 608 575 513 494 383 278 117
65 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 973 1069 4008
Size: 787 186 96 2939
Fragments arranged by size:
2939 787 186 96
BsiHKAI G_wGCw'C
Cuts at: 0 254 554 1664 1818 2065 2342 2396 2861
Size: 254 300 1110 154 247 277 54 465
Cuts at: 2861 4008
Size: 1147
Fragments arranged by size:
1147 1110 465 300 277 254 247 154
54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 371 475 3384 4008
Size: 371 104 2909 624
Fragments arranged by size:
2909 624 371 104
Bsp1286I G_dGCh'C
Cuts at: 0 254 445 554 1007 1305 1664 1771 1818
Size: 254 191 109 453 298 359 107 47
Cuts at: 1818 1915 2065 2342 2396 2779 2861 2951 3002
Size: 97 150 277 54 383 82 90 51
Cuts at: 3002 3041 3452 4008
Size: 39 411 556
Fragments arranged by size:
556 453 411 383 359 298 277 254
191 150 109 107 97 90 82 54
51 47 39
BspEI T'CCGG_A
Cuts at: 0 3524 3635 4008
Size: 3524 111 373
Fragments arranged by size:
3524 373 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1398 2234 2295 2318 3779 4008
Size: 1398 836 61 23 1461 229
Fragments arranged by size:
1461 1398 836 229 61 23
BsrBI CCG'CTC
Cuts at: 0 2732 4008
Size: 2732 1276
BsrDI GCAATG_nn'
Cuts at: 0 650 2079 4008
Size: 650 1429 1929
Fragments arranged by size:
1929 1429 650
BsrFI r'CCGG_y
Cuts at: 0 401 1483 2173 3437 4008
Size: 401 1082 690 1264 571
Fragments arranged by size:
1264 1082 690 571 401
BsrGI T'GTAC_A
Cuts at: 0 509 4008
Size: 509 3499
BssSI C'ACGA_G
Cuts at: 0 2866 4008
Size: 2866 1142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1455 2147 4008
Size: 1455 692 1861
Fragments arranged by size:
1861 1455 692
BstYI r'GATC_y
Cuts at: 0 22 935 1019 2536 2870 3576 4008
Size: 22 913 84 1517 334 706 432
Fragments arranged by size:
1517 913 706 432 334 84 22
Bsu36I CC'TnA_GG
Cuts at: 0 1256 2398 3898 4008
Size: 1256 1142 1500 110
Fragments arranged by size:
1500 1256 1142 110
BtgI C'CryG_G
Cuts at: 0 195 257 1496 1778 2747 4008
Size: 195 62 1239 282 969 1261
Fragments arranged by size:
1261 1239 969 282 195 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1848 1938 2441 4008
Size: 1848 90 503 1567
Fragments arranged by size:
1848 1567 503 90
BtsI GCAGTG_nn'
Cuts at: 0 1954 3324 3776 4008
Size: 1954 1370 452 232
Fragments arranged by size:
1954 1370 452 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3855 3890 4008
Size: 3855 35 118
Fragments arranged by size:
3855 118 35
EaeI y'GGCC_r
Cuts at: 0 116 1457 1685 1714 1790 2642 2894 3130
Size: 116 1341 228 29 76 852 252 236
Cuts at: 3130 3212 4008
Size: 82 796
Fragments arranged by size:
1341 852 796 252 236 228 116 82
76 29
EarI CTCTTCn'nnn_
Cuts at: 0 1212 2018 4008
Size: 1212 806 1990
Fragments arranged by size:
1990 1212 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 992 3691 4008
Size: 992 2699 317
Fragments arranged by size:
2699 992 317
EcoICRI GAG'CTC
Cuts at: 0 1816 2859 4008
Size: 1816 1043 1149
Fragments arranged by size:
1816 1149 1043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 229 568 640 919 1120 1243 1561
Size: 186 43 339 72 279 201 123 318
Cuts at: 1561 1654 1675 2718 2973 3204 3492 4008
Size: 93 21 1043 255 231 288 516
Fragments arranged by size:
1043 516 339 318 288 279 255 231
201 186 123 93 72 43 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1141 2242 4008
Size: 1141 1101 1766
Fragments arranged by size:
1766 1141 1101
EcoO109I rG'GnC_Cy
Cuts at: 0 1365 1545 1617 1698 1911 1912 2501 2752
Size: 1365 180 72 81 213 1 589 251
Cuts at: 2752 2775 3037 3269 4008
Size: 23 262 232 739
Fragments arranged by size:
1365 739 589 262 251 232 213 180
81 72 23 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2605 4008
Size: 2605 1403
FspI TGC'GCA
Cuts at: 0 2374 3049 4008
Size: 2374 675 959
Fragments arranged by size:
2374 959 675
HaeII r_GCGC'y
Cuts at: 0 129 817 1131 1827 2320 2641 2664 2701
Size: 129 688 314 696 493 321 23 37
Cuts at: 2701 3120 4008
Size: 419 888
Fragments arranged by size:
888 696 688 493 419 321 314 129
37 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 1189 1221 2326 2358 2526 2528
Size: 151 32 1006 32 1105 32 168 2
Cuts at: 2528 2558 2560 3362 3394 3568 3600 3606 3638
Size: 30 2 802 32 174 32 6 32
Cuts at: 3638 4008
Size: 370
Fragments arranged by size:
1105 1006 802 370 174 168 151 32
32 32 32 32 32 30 6 2
2
HincII GTy'rAC
Cuts at: 0 2127 4008
Size: 2127 1881
HindIII A'AGCT_T
Cuts at: 0 217 2587 4008
Size: 217 2370 1421
Fragments arranged by size:
2370 1421 217
KasI G'GCGC_C
Cuts at: 0 1823 2316 2697 4008
Size: 1823 493 381 1311
Fragments arranged by size:
1823 1311 493 381
KpnI G_GTAC'C
Cuts at: 0 756 4008
Size: 756 3252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 592 820 913 1409 2246 2312 2903
Size: 149 443 228 93 496 837 66 591
Cuts at: 2903 4008
Size: 1105
Fragments arranged by size:
1105 837 591 496 443 228 149 93
66
MscI TGG'CCA
Cuts at: 0 1687 1716 2644 2896 4008
Size: 1687 29 928 252 1112
Fragments arranged by size:
1687 1112 928 252 29
MslI CAynn'nnrTG
Cuts at: 0 448 1667 1978 2053 4008
Size: 448 1219 311 75 1955
Fragments arranged by size:
1955 1219 448 311 75
MspA1I CmG'CkG
Cuts at: 0 197 231 498 1014 1780 2749 3033 3281
Size: 197 34 267 516 766 969 284 248
Cuts at: 3281 3395 3420 3519 4008
Size: 114 25 99 489
Fragments arranged by size:
969 766 516 489 284 267 248 197
114 99 34 25
NaeI GCC'GGC
Cuts at: 0 403 2175 4008
Size: 403 1772 1833
Fragments arranged by size:
1833 1772 403
NarI GG'CG_CC
Cuts at: 0 1824 2317 2698 4008
Size: 1824 493 381 1310
Fragments arranged by size:
1824 1310 493 381
NcoI C'CATG_G
Cuts at: 0 257 4008
Size: 257 3751
NgoMIV G'CCGG_C
Cuts at: 0 401 2173 4008
Size: 401 1772 1835
Fragments arranged by size:
1835 1772 401
NspI r_CATG'y
Cuts at: 0 58 459 606 3258 3711 4008
Size: 58 401 147 2652 453 297
Fragments arranged by size:
2652 453 401 297 147 58
PciI A'CATG_T
Cuts at: 0 602 4008
Size: 602 3406
PflMI CCAn_nnn'nTGG
Cuts at: 0 3546 4008
Size: 3546 462
PfoI T'CCnGG_A
Cuts at: 0 1097 1580 1631 2611 3452 4008
Size: 1097 483 51 980 841 556
Fragments arranged by size:
1097 980 841 556 483 51
PpuMI rG'GwC_Cy
Cuts at: 0 1617 1698 2752 3269 4008
Size: 1617 81 1054 517 739
Fragments arranged by size:
1617 1054 739 517 81
PshAI GACnn'nnGTC
Cuts at: 0 1679 4008
Size: 1679 2329
PspOMI G'GGCC_C
Cuts at: 0 1767 1911 2775 3037 4008
Size: 1767 144 864 262 971
Fragments arranged by size:
1767 971 864 262 144
PstI C_TGCA'G
Cuts at: 0 30 3268 4008
Size: 30 3238 740
Fragments arranged by size:
3238 740 30
PvuII CAG'CTG
Cuts at: 0 231 1014 3033 3395 3420 4008
Size: 231 783 2019 362 25 588
Fragments arranged by size:
2019 783 588 362 231 25
RsrII CG'GwC_CG
Cuts at: 0 1862 4008
Size: 1862 2146
SacI G_AGCT'C
Cuts at: 0 1818 2861 4008
Size: 1818 1043 1147
Fragments arranged by size:
1818 1147 1043
SacII CC_GC'GG
Cuts at: 0 198 1781 2750 4008
Size: 198 1583 969 1258
Fragments arranged by size:
1583 1258 969 198
SanDI GG'GwC_CC
Cuts at: 0 1698 2752 4008
Size: 1698 1054 1256
Fragments arranged by size:
1698 1256 1054
ScaI AGT'ACT
Cuts at: 0 685 4008
Size: 685 3323
SexAI A'CCwGG_T
Cuts at: 0 581 4008
Size: 581 3427
SfcI C'TryA_G
Cuts at: 0 26 956 3264 4008
Size: 26 930 2308 744
Fragments arranged by size:
2308 930 744 26
SfoI GGC'GCC
Cuts at: 0 1825 2318 2699 4008
Size: 1825 493 381 1309
Fragments arranged by size:
1825 1309 493 381
SmaI CCC'GGG
Cuts at: 0 1917 2963 3016 4008
Size: 1917 1046 53 992
Fragments arranged by size:
1917 1046 992 53
SmlI C'TyrA_G
Cuts at: 0 2098 2242 4008
Size: 2098 144 1766
Fragments arranged by size:
2098 1766 144
SnaBI TAC'GTA
Cuts at: 0 288 4008
Size: 288 3720
SphI G_CATG'C
Cuts at: 0 3258 4008
Size: 3258 750
StuI AGG'CCT
Cuts at: 0 1240 1852 3665 4008
Size: 1240 612 1813 343
Fragments arranged by size:
1813 1240 612 343
StyI C'CwwG_G
Cuts at: 0 257 1754 3920 4008
Size: 257 1497 2166 88
Fragments arranged by size:
2166 1497 257 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 582 819 1320 1350 1879 3534 4008
Size: 448 134 237 501 30 529 1655 474
Fragments arranged by size:
1655 529 501 474 448 237 134 30
TatI w'GTAC_w
Cuts at: 0 509 683 1112 4008
Size: 509 174 429 2896
Fragments arranged by size:
2896 509 429 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 589 621 857 889 1473 1505 2292 2324
Size: 589 32 236 32 584 32 787 32
Cuts at: 2324 2716 2748 3526 3558 4008
Size: 392 32 778 32 450
Fragments arranged by size:
787 778 589 584 450 392 236 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1416 4008
Size: 1416 2592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 1761 2434 4008
Size: 730 1031 673 1574
Fragments arranged by size:
1574 1031 730 673
XmaI C'CCGG_G
Cuts at: 0 1915 2961 3014 4008
Size: 1915 1046 53 994
Fragments arranged by size:
1915 1046 994 53
XmnI GAAnn'nnTTC
Cuts at: 0 1903 4008
Size: 1903 2105
ZraI GAC'GTC
Cuts at: 0 381 4008
Size: 381 3627
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII RsrII SacI SacII SanDI ScaI
SexAI SfcI SfoI SmaI SmlI SnaBI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AgeI AloI AscI AseI AsiSI AvrII BaeI
BfrBI BglII BmtI BsaBI BsiEI BsiWI BsmI BspHI
BssHII BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspAI HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI SalI
SapI SbfI SfiI SgfI SgrAI SpeI SrfI SspI
SwaI XbaI XhoI