Restriction map for
pFN21AE9621
(Linear) (Six-base) MAPSORT of: KIEE9621.seq Check: 4317 from: 1 to: 1668
KIEE9621.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 836 1444 1668
Size: 149 687 608 224
Fragments arranged by size:
687 608 224 149
AflIII A'CryG_T
Cuts at: 0 12 1668
Size: 12 1656
AleI CACnn'nnGTG
Cuts at: 0 13 1668
Size: 13 1655
ApoI r'AATT_y
Cuts at: 0 128 297 337 435 488 531 704 1561
Size: 128 169 40 98 53 43 173 857
Cuts at: 1561 1668
Size: 107
Fragments arranged by size:
857 173 169 128 107 98 53 43
40
BanII G_rGCy'C
Cuts at: 0 917 1668
Size: 917 751
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 689 1668
Size: 504 185 979
Fragments arranged by size:
979 504 185
BbvCI CC'TCA_GC
Cuts at: 0 1340 1668
Size: 1340 328
BciVI GTATCCnnnnn_n'
Cuts at: 0 1275 1668
Size: 1275 393
BclI T'GATC_A
Cuts at: 0 663 1668
Size: 663 1005
BfrBI ATG'CAT
Cuts at: 0 690 1668
Size: 690 978
BlpI GC'TnA_GC
Cuts at: 0 1606 1668
Size: 1606 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 1668
Size: 75 1593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1668
Size: 596 1072
Bpu10I CC'TnA_GC
Cuts at: 0 1340 1668
Size: 1340 328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 900 1037 1668
Size: 900 137 631
Fragments arranged by size:
900 631 137
BsiHKAI G_wGCw'C
Cuts at: 0 917 1620 1668
Size: 917 703 48
Fragments arranged by size:
917 703 48
BsmI GAATG_Cn'
Cuts at: 0 372 1505 1668
Size: 372 1133 163
Fragments arranged by size:
1133 372 163
Bsp1286I G_dGCh'C
Cuts at: 0 917 1620 1668
Size: 917 703 48
Fragments arranged by size:
917 703 48
BspHI T'CATG_A
Cuts at: 0 342 1275 1668
Size: 342 933 393
Fragments arranged by size:
933 393 342
BsrDI GCAATG_nn'
Cuts at: 0 325 857 1422 1668
Size: 325 532 565 246
Fragments arranged by size:
565 532 325 246
BstBI TT'CG_AA
Cuts at: 0 159 192 1668
Size: 159 33 1476
Fragments arranged by size:
1476 159 33
BstYI r'GATC_y
Cuts at: 0 900 1258 1668
Size: 900 358 410
Fragments arranged by size:
900 410 358
BtsI GCAGTG_nn'
Cuts at: 0 240 1668
Size: 240 1428
DraI TTT'AAA
Cuts at: 0 1116 1668
Size: 1116 552
EaeI y'GGCC_r
Cuts at: 0 41 1668
Size: 41 1627
EarI CTCTTCn'nnn_
Cuts at: 0 812 1009 1668
Size: 812 197 659
Fragments arranged by size:
812 659 197
EcoICRI GAG'CTC
Cuts at: 0 915 1668
Size: 915 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 596 836 1444 1668
Size: 149 447 240 608 224
Fragments arranged by size:
608 447 240 224 149
EcoRI G'AATT_C
Cuts at: 0 531 1668
Size: 531 1137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 262 1668
Size: 262 1406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 633 665 1333 1365 1455 1487 1668
Size: 633 32 668 32 90 32 181
Fragments arranged by size:
668 633 181 90 32 32 32
HincII GTy'rAC
Cuts at: 0 282 1043 1668
Size: 282 761 625
Fragments arranged by size:
761 625 282
MfeI C'AATT_G
Cuts at: 0 842 1668
Size: 842 826
MscI TGG'CCA
Cuts at: 0 43 1668
Size: 43 1625
MslI CAynn'nnrTG
Cuts at: 0 13 964 1668
Size: 13 951 704
Fragments arranged by size:
951 704 13
MspA1I CmG'CkG
Cuts at: 0 1555 1593 1668
Size: 1555 38 75
Fragments arranged by size:
1555 75 38
NsiI A_TGCA'T
Cuts at: 0 692 1668
Size: 692 976
NspI r_CATG'y
Cuts at: 0 16 690 1668
Size: 16 674 978
Fragments arranged by size:
978 674 16
PciI A'CATG_T
Cuts at: 0 12 1668
Size: 12 1656
PflMI CCAn_nnn'nTGG
Cuts at: 0 50 1668
Size: 50 1618
PsiI TTA'TAA
Cuts at: 0 1661 1668
Size: 1661 7
PstI C_TGCA'G
Cuts at: 0 633 1668
Size: 633 1035
PvuII CAG'CTG
Cuts at: 0 1555 1593 1668
Size: 1555 38 75
Fragments arranged by size:
1555 75 38
SacI G_AGCT'C
Cuts at: 0 917 1668
Size: 917 751
SfcI C'TryA_G
Cuts at: 0 629 1527 1668
Size: 629 898 141
Fragments arranged by size:
898 629 141
SspI AAT'ATT
Cuts at: 0 803 1296 1428 1668
Size: 803 493 132 240
Fragments arranged by size:
803 493 240 132
StyI C'CwwG_G
Cuts at: 0 140 1668
Size: 140 1528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 370 1556 1668
Size: 153 217 1186 112
Fragments arranged by size:
1186 217 153 112
XbaI T'CTAG_A
Cuts at: 0 108 1668
Size: 108 1560
XmnI GAAnn'nnTTC
Cuts at: 0 128 242 338 1668
Size: 128 114 96 1330
Fragments arranged by size:
1330 128 114 96
Enzymes that do cut:
AcuI AflIII AleI ApoI BanII BbsI BbvCI BciVI
BclI BfrBI BlpI BmrI BpmI Bpu10I BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BstBI BstYI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoRI FalI Hin4I HincII
MfeI MscI MslI MspA1I NsiI NspI PciI PflMI
PsiI PstI PvuII SacI SfcI SspI StyI TaqII
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BglI BglII Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI Tth111I XcmI XhoI
XmaI ZraI