Restriction map for
pFN21AE9618
(Linear) (Six-base) MAPSORT of: KIEE9618.seq Check: 3974 from: 1 to: 1197
KIEE9618.orf
With 182 enzymes: SgfI *
March 22, 2012 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 772 1197
Size: 772 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1197
Size: 453 744
AlwNI CAG_nnn'CTG
Cuts at: 0 407 1197
Size: 407 790
ApaI G_GGCC'C
Cuts at: 0 168 1049 1197
Size: 168 881 148
Fragments arranged by size:
881 168 148
AscI GG'CGCG_CC
Cuts at: 0 18 190 1197
Size: 18 172 1007
Fragments arranged by size:
1007 172 18
AvaI C'yCGr_G
Cuts at: 0 217 526 1197
Size: 217 309 671
Fragments arranged by size:
671 309 217
BanI G'GyrC_C
Cuts at: 0 222 1197
Size: 222 975
BanII G_rGCy'C
Cuts at: 0 168 179 1009 1049 1197
Size: 168 11 830 40 148
Fragments arranged by size:
830 168 148 40 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 155 1197
Size: 121 34 1042
Fragments arranged by size:
1042 121 34
BglII A'GATC_T
Cuts at: 0 936 1197
Size: 936 261
BlpI GC'TnA_GC
Cuts at: 0 386 1197
Size: 386 811
Bme1580I G_kGCm'C
Cuts at: 0 168 227 1049 1197
Size: 168 59 822 148
Fragments arranged by size:
822 168 148 59
BmgBI CAC'GTC
Cuts at: 0 813 1077 1095 1197
Size: 813 264 18 102
Fragments arranged by size:
813 264 102 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 257 446 1181 1197
Size: 257 189 735 16
Fragments arranged by size:
735 257 189 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 985 1197
Size: 487 498 212
Fragments arranged by size:
498 487 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1197
Size: 718 479
BsaWI w'CCGG_w
Cuts at: 0 236 1197
Size: 236 961
BseYI C'CCAG_C
Cuts at: 0 1082 1086 1197
Size: 1082 4 111
Fragments arranged by size:
1082 111 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 831 1180 1197
Size: 750 81 349 17
Fragments arranged by size:
750 349 81 17
BsiHKAI G_wGCw'C
Cuts at: 0 1009 1197
Size: 1009 188
BsmI GAATG_Cn'
Cuts at: 0 232 458 1197
Size: 232 226 739
Fragments arranged by size:
739 232 226
Bsp1286I G_dGCh'C
Cuts at: 0 168 179 227 1009 1049 1197
Size: 168 11 48 782 40 148
Fragments arranged by size:
782 168 148 48 40 11
BspEI T'CCGG_A
Cuts at: 0 236 1197
Size: 236 961
BspHI T'CATG_A
Cuts at: 0 648 1197
Size: 648 549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 772 1197
Size: 772 425
BsrFI r'CCGG_y
Cuts at: 0 436 850 1197
Size: 436 414 347
Fragments arranged by size:
436 414 347
BsrGI T'GTAC_A
Cuts at: 0 491 1197
Size: 491 706
BssHII G'CGCG_C
Cuts at: 0 18 190 1197
Size: 18 172 1007
Fragments arranged by size:
1007 172 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 773 1197
Size: 773 424
BstYI r'GATC_y
Cuts at: 0 936 1197
Size: 936 261
BtgI C'CryG_G
Cuts at: 0 23 159 168 929 1197
Size: 23 136 9 761 268
Fragments arranged by size:
761 268 136 23 9
BtsI GCAGTG_nn'
Cuts at: 0 357 1197
Size: 357 840
ClaI AT'CG_AT
Cuts at: 0 689 1197
Size: 689 508
DraIII CAC_nnn'GTG
Cuts at: 0 454 1107 1197
Size: 454 653 90
Fragments arranged by size:
653 454 90
EaeI y'GGCC_r
Cuts at: 0 584 1197
Size: 584 613
EarI CTCTTCn'nnn_
Cuts at: 0 1136 1197
Size: 1136 61
EcoICRI GAG'CTC
Cuts at: 0 1007 1197
Size: 1007 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 487 985 1197
Size: 453 34 498 212
Fragments arranged by size:
498 453 212 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 201 411 1197
Size: 201 210 786
Fragments arranged by size:
786 210 201
EcoO109I rG'GnC_Cy
Cuts at: 0 164 956 1045 1046 1152 1197
Size: 164 792 89 1 106 45
Fragments arranged by size:
792 164 106 89 45 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 691 723 1010 1042 1197
Size: 457 32 202 32 287 32 155
Fragments arranged by size:
457 287 202 155 32 32 32
HindIII A'AGCT_T
Cuts at: 0 364 1177 1197
Size: 364 813 20
Fragments arranged by size:
813 364 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1197
Size: 315 882
MscI TGG'CCA
Cuts at: 0 586 1197
Size: 586 611
MspA1I CmG'CkG
Cuts at: 0 25 170 836 891 1086 1164 1197
Size: 25 145 666 55 195 78 33
Fragments arranged by size:
666 195 145 78 55 33 25
NaeI GCC'GGC
Cuts at: 0 438 1197
Size: 438 759
NcoI C'CATG_G
Cuts at: 0 159 929 1197
Size: 159 770 268
Fragments arranged by size:
770 268 159
NgoMIV G'CCGG_C
Cuts at: 0 436 1197
Size: 436 761
NspI r_CATG'y
Cuts at: 0 916 1197
Size: 916 281
PasI CC'CwG_GG
Cuts at: 0 227 790 1197
Size: 227 563 407
Fragments arranged by size:
563 407 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 1197
Size: 417 780
PpuMI rG'GwC_Cy
Cuts at: 0 956 1152 1197
Size: 956 196 45
Fragments arranged by size:
956 196 45
PspOMI G'GGCC_C
Cuts at: 0 164 1045 1197
Size: 164 881 152
Fragments arranged by size:
881 164 152
PstI C_TGCA'G
Cuts at: 0 377 1197
Size: 377 820
PvuII CAG'CTG
Cuts at: 0 1086 1197
Size: 1086 111
SacI G_AGCT'C
Cuts at: 0 1009 1197
Size: 1009 188
SacII CC_GC'GG
Cuts at: 0 26 171 1197
Size: 26 145 1026
Fragments arranged by size:
1026 145 26
SbfI CC_TGCA'GG
Cuts at: 0 377 1197
Size: 377 820
SfcI C'TryA_G
Cuts at: 0 373 1197
Size: 373 824
SgrAI Cr'CCGG_yG
Cuts at: 0 850 1197
Size: 850 347
SmlI C'TyrA_G
Cuts at: 0 697 1197
Size: 697 500
StuI AGG'CCT
Cuts at: 0 97 207 1197
Size: 97 110 990
Fragments arranged by size:
990 110 97
StyI C'CwwG_G
Cuts at: 0 159 179 587 929 951 1049 1197
Size: 159 20 408 342 22 98 148
Fragments arranged by size:
408 342 159 148 98 22 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 465 1006 1197
Size: 307 158 541 191
Fragments arranged by size:
541 307 191 158
TatI w'GTAC_w
Cuts at: 0 491 1197
Size: 491 706
Enzymes that do cut:
AarI AcuI AlwNI ApaI AscI AvaI BanI BanII
BcgI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtsI
ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HindIII MmeI MscI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PpuMI PspOMI PstI PvuII SacI
SacII SbfI SfcI SgrAI SmlI StuI StyI TaqII
TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BsiEI BsiWI BsmBI BsrBI BsrDI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MslI NarI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI