Restriction map for
pFN21AE9617
(Linear) (Six-base) MAPSORT of: KIEE9617.seq Check: 8688 from: 1 to: 1032
KIEE9617.orf
With 182 enzymes: SgfI *
March 22, 2012 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 920 1032
Size: 71 849 112
Fragments arranged by size:
849 112 71
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 96 128 1032
Size: 96 32 904
Fragments arranged by size:
904 96 32
ApaLI G'TGCA_C
Cuts at: 0 14 1032
Size: 14 1018
ApoI r'AATT_y
Cuts at: 0 143 244 712 887 1032
Size: 143 101 468 175 145
Fragments arranged by size:
468 175 145 143 101
AvrII C'CTAG_G
Cuts at: 0 372 1032
Size: 372 660
BanI G'GyrC_C
Cuts at: 0 67 1032
Size: 67 965
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 499 1032
Size: 344 155 533
Fragments arranged by size:
533 344 155
BfrBI ATG'CAT
Cuts at: 0 643 1032
Size: 643 389
Bme1580I G_kGCm'C
Cuts at: 0 18 1032
Size: 18 1014
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1032
Size: 144 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1032
Size: 532 500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 1032
Size: 27 1005
BsaBI GATnn'nnATC
Cuts at: 0 93 1032
Size: 93 939
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 1032
Size: 65 967
BsiHKAI G_wGCw'C
Cuts at: 0 18 1032
Size: 18 1014
BsmI GAATG_Cn'
Cuts at: 0 193 1032
Size: 193 839
Bsp1286I G_dGCh'C
Cuts at: 0 18 1032
Size: 18 1014
BstAPI GCAn_nnn'nTGC
Cuts at: 0 640 1032
Size: 640 392
BstEII G'GTnAC_C
Cuts at: 0 822 1032
Size: 822 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 532 920 1032
Size: 71 461 388 112
Fragments arranged by size:
461 388 112 71
EcoO109I rG'GnC_Cy
Cuts at: 0 396 1032
Size: 396 636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 427 1032
Size: 427 605
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 954 986 1032
Size: 117 32 805 32 46
Fragments arranged by size:
805 117 46 32 32
HincII GTy'rAC
Cuts at: 0 26 177 840 1032
Size: 26 151 663 192
Fragments arranged by size:
663 192 151 26
MfeI C'AATT_G
Cuts at: 0 436 1032
Size: 436 596
MslI CAynn'nnrTG
Cuts at: 0 352 947 1032
Size: 352 595 85
Fragments arranged by size:
595 352 85
NsiI A_TGCA'T
Cuts at: 0 645 1032
Size: 645 387
NspI r_CATG'y
Cuts at: 0 683 1032
Size: 683 349
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1032
Size: 699 333
PfoI T'CCnGG_A
Cuts at: 0 655 1032
Size: 655 377
PpuMI rG'GwC_Cy
Cuts at: 0 396 1032
Size: 396 636
PstI C_TGCA'G
Cuts at: 0 83 1032
Size: 83 949
SanDI GG'GwC_CC
Cuts at: 0 396 1032
Size: 396 636
SfcI C'TryA_G
Cuts at: 0 79 252 732 929 1032
Size: 79 173 480 197 103
Fragments arranged by size:
480 197 173 103 79
SmlI C'TyrA_G
Cuts at: 0 42 1032
Size: 42 990
SphI G_CATG'C
Cuts at: 0 683 1032
Size: 683 349
StyI C'CwwG_G
Cuts at: 0 372 1032
Size: 372 660
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1032
Size: 87 945
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 1032
Size: 308 32 692
Fragments arranged by size:
692 308 32
Tth111I GACn'n_nGTC
Cuts at: 0 799 1032
Size: 799 233
XbaI T'CTAG_A
Cuts at: 0 274 1032
Size: 274 758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 811 1032
Size: 811 221
XmnI GAAnn'nnTTC
Cuts at: 0 407 1032
Size: 407 625
Enzymes that do cut:
AcuI AloI ApaLI ApoI AvrII BanI BbsI BfrBI
Bme1580I BmrI BpmI BpuEI BsaBI BseRI BsiHKAI BsmI
Bsp1286I BstAPI BstEII Eco57MI EcoO109I FalI Hin4I HincII
MfeI MslI NsiI NspI PflMI PfoI PpuMI PstI
SanDI SfcI SmlI SphI StyI TaqII TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI AscI AseI
AsiSI AvaI BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI XhoI XmaI
ZraI