Restriction map for
pFN21AE9613
(Linear) (Six-base) MAPSORT of: KIEE9613.seq Check: 658 from: 1 to: 942
KIEE9613.orf
With 182 enzymes: SgfI *
March 22, 2012 18:34 ..
AatII G_ACGT'C
Cuts at: 0 614 942
Size: 614 328
AccI GT'mk_AC
Cuts at: 0 794 942
Size: 794 148
AfeI AGC'GCT
Cuts at: 0 145 942
Size: 145 797
AhdI GACnn_n'nnGTC
Cuts at: 0 222 942
Size: 222 720
AleI CACnn'nnGTG
Cuts at: 0 743 942
Size: 743 199
ApaI G_GGCC'C
Cuts at: 0 866 942
Size: 866 76
ApaLI G'TGCA_C
Cuts at: 0 736 942
Size: 736 206
ApoI r'AATT_y
Cuts at: 0 900 942
Size: 900 42
AvaI C'yCGr_G
Cuts at: 0 530 942
Size: 530 412
BanI G'GyrC_C
Cuts at: 0 464 942
Size: 464 478
BanII G_rGCy'C
Cuts at: 0 479 866 942
Size: 479 387 76
Fragments arranged by size:
479 387 76
BbeI G_GCGC'C
Cuts at: 0 468 942
Size: 468 474
BbvCI CC'TCA_GC
Cuts at: 0 581 942
Size: 581 361
BciVI GTATCCnnnnn_n'
Cuts at: 0 203 942
Size: 203 739
BfrBI ATG'CAT
Cuts at: 0 488 942
Size: 488 454
Bme1580I G_kGCm'C
Cuts at: 0 297 740 866 942
Size: 297 443 126 76
Fragments arranged by size:
443 297 126 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 324 384 942
Size: 191 133 60 558
Fragments arranged by size:
558 191 133 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 942
Size: 594 348
Bpu10I CC'TnA_GC
Cuts at: 0 581 942
Size: 581 361
BsaHI Gr'CG_yC
Cuts at: 0 465 611 942
Size: 465 146 331
Fragments arranged by size:
465 331 146
BsaWI w'CCGG_w
Cuts at: 0 287 635 942
Size: 287 348 307
Fragments arranged by size:
348 307 287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 101 339 640 942
Size: 101 238 301 302
Fragments arranged by size:
302 301 238 101
BseYI C'CCAG_C
Cuts at: 0 58 942
Size: 58 884
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 942
Size: 353 589
BsiEI CG_ry'CG
Cuts at: 0 874 942
Size: 874 68
BsiHKAI G_wGCw'C
Cuts at: 0 73 388 479 740 942
Size: 73 315 91 261 202
Fragments arranged by size:
315 261 202 91 73
Bsp1286I G_dGCh'C
Cuts at: 0 73 297 388 479 740 866 942
Size: 73 224 91 91 261 126 76
Fragments arranged by size:
261 224 126 91 91 76 73
BspEI T'CCGG_A
Cuts at: 0 287 635 942
Size: 287 348 307
Fragments arranged by size:
348 307 287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 664 780 942
Size: 664 116 162
Fragments arranged by size:
664 162 116
BstEII G'GTnAC_C
Cuts at: 0 420 942
Size: 420 522
BstZ17I GTA'TAC
Cuts at: 0 795 942
Size: 795 147
BtgI C'CryG_G
Cuts at: 0 480 588 942
Size: 480 108 354
Fragments arranged by size:
480 354 108
BtsI GCAGTG_nn'
Cuts at: 0 818 942
Size: 818 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 942
Size: 27 915
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 292 942
Size: 292 650
EcoICRI GAG'CTC
Cuts at: 0 477 942
Size: 477 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 942
Size: 594 348
EcoO109I rG'GnC_Cy
Cuts at: 0 498 648 942
Size: 498 150 294
Fragments arranged by size:
498 294 150
EcoRI G'AATT_C
Cuts at: 0 900 942
Size: 900 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 256 942
Size: 27 229 686
Fragments arranged by size:
686 229 27
HaeII r_GCGC'y
Cuts at: 0 147 468 942
Size: 147 321 474
Fragments arranged by size:
474 321 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 942
Size: 208 32 702
Fragments arranged by size:
702 208 32
HincII GTy'rAC
Cuts at: 0 20 942
Size: 20 922
KasI G'GCGC_C
Cuts at: 0 464 942
Size: 464 478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 854 942
Size: 25 829 88
Fragments arranged by size:
829 88 25
MslI CAynn'nnrTG
Cuts at: 0 743 942
Size: 743 199
MspA1I CmG'CkG
Cuts at: 0 309 942
Size: 309 633
NarI GG'CG_CC
Cuts at: 0 465 942
Size: 465 477
NcoI C'CATG_G
Cuts at: 0 588 942
Size: 588 354
NsiI A_TGCA'T
Cuts at: 0 490 942
Size: 490 452
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 594 942
Size: 432 162 348
Fragments arranged by size:
432 348 162
PpuMI rG'GwC_Cy
Cuts at: 0 498 648 942
Size: 498 150 294
Fragments arranged by size:
498 294 150
PspOMI G'GGCC_C
Cuts at: 0 862 942
Size: 862 80
SacI G_AGCT'C
Cuts at: 0 479 942
Size: 479 463
SfcI C'TryA_G
Cuts at: 0 219 942
Size: 219 723
SfoI GGC'GCC
Cuts at: 0 466 942
Size: 466 476
SmaI CCC'GGG
Cuts at: 0 532 942
Size: 532 410
StyI C'CwwG_G
Cuts at: 0 449 588 942
Size: 449 139 354
Fragments arranged by size:
449 354 139
TatI w'GTAC_w
Cuts at: 0 410 942
Size: 410 532
Tth111I GACn'n_nGTC
Cuts at: 0 496 942
Size: 496 446
XmaI C'CCGG_G
Cuts at: 0 530 942
Size: 530 412
ZraI GAC'GTC
Cuts at: 0 612 942
Size: 612 330
Enzymes that do cut:
AatII AccI AfeI AhdI AleI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbvCI BciVI BfrBI Bme1580I
BmrI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BstEII BstZ17I BtgI
BtsI DrdI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI
NcoI NsiI PflMI PpuMI PspOMI SacI SfcI SfoI
SmaI StyI TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BclI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI ClaI CspCI
DraI DraIII EaeI EagI EarI EcoNI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XcmI XhoI XmnI