Restriction map for
pFN21AE9611
(Linear) (Six-base) MAPSORT of: KIEE9611.seq Check: 1481 from: 1 to: 900
KIEE9611.orf
With 182 enzymes: SgfI *
March 22, 2012 18:33 ..
AatII G_ACGT'C
Cuts at: 0 749 900
Size: 749 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 574 900
Size: 125 449 326
Fragments arranged by size:
449 326 125
AflIII A'CryG_T
Cuts at: 0 757 900
Size: 757 143
AlwNI CAG_nnn'CTG
Cuts at: 0 539 900
Size: 539 361
ApaI G_GGCC'C
Cuts at: 0 623 900
Size: 623 277
BamHI G'GATC_C
Cuts at: 0 672 900
Size: 672 228
BanI G'GyrC_C
Cuts at: 0 200 900
Size: 200 700
BanII G_rGCy'C
Cuts at: 0 623 900
Size: 623 277
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 900
Size: 462 438
BbvCI CC'TCA_GC
Cuts at: 0 184 900
Size: 184 716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 900
Size: 819 34 47
Fragments arranged by size:
819 47 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 440 900
Size: 440 460
Bme1580I G_kGCm'C
Cuts at: 0 623 900
Size: 623 277
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 626 900
Size: 460 166 274
Fragments arranged by size:
460 274 166
Bpu10I CC'TnA_GC
Cuts at: 0 184 900
Size: 184 716
BsaI GGTCTCn'nnnn_
Cuts at: 0 67 900
Size: 67 833
BsaHI Gr'CG_yC
Cuts at: 0 168 746 900
Size: 168 578 154
Fragments arranged by size:
578 168 154
BsaWI w'CCGG_w
Cuts at: 0 443 900
Size: 443 457
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 758 788 900
Size: 353 30 375 30 112
Fragments arranged by size:
375 353 112 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 808 900
Size: 374 434 92
Fragments arranged by size:
434 374 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 900
Size: 142 758
BsiHKAI G_wGCw'C
Cuts at: 0 141 900
Size: 141 759
BsmI GAATG_Cn'
Cuts at: 0 558 900
Size: 558 342
Bsp1286I G_dGCh'C
Cuts at: 0 141 623 900
Size: 141 482 277
Fragments arranged by size:
482 277 141
BspEI T'CCGG_A
Cuts at: 0 443 900
Size: 443 457
BspHI T'CATG_A
Cuts at: 0 810 900
Size: 810 90
BsrFI r'CCGG_y
Cuts at: 0 203 584 900
Size: 203 381 316
Fragments arranged by size:
381 316 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 120 229 900
Size: 120 109 671
Fragments arranged by size:
671 120 109
BstYI r'GATC_y
Cuts at: 0 88 672 900
Size: 88 584 228
Fragments arranged by size:
584 228 88
EarI CTCTTCn'nnn_
Cuts at: 0 101 278 479 740 900
Size: 101 177 201 261 160
Fragments arranged by size:
261 201 177 160 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 574 900
Size: 125 449 326
Fragments arranged by size:
449 326 125
EcoO109I rG'GnC_Cy
Cuts at: 0 620 720 900
Size: 620 100 180
Fragments arranged by size:
620 180 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 157 900
Size: 157 743
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 353 385 900
Size: 19 32 302 32 515
Fragments arranged by size:
515 302 32 32 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 900
Size: 860 40
NspI r_CATG'y
Cuts at: 0 227 761 900
Size: 227 534 139
Fragments arranged by size:
534 227 139
PciI A'CATG_T
Cuts at: 0 757 900
Size: 757 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 788 900
Size: 255 533 112
Fragments arranged by size:
533 255 112
PpuMI rG'GwC_Cy
Cuts at: 0 720 900
Size: 720 180
PspOMI G'GGCC_C
Cuts at: 0 619 900
Size: 619 281
PstI C_TGCA'G
Cuts at: 0 116 317 900
Size: 116 201 583
Fragments arranged by size:
583 201 116
SapI GCTCTTCn'nnn_
Cuts at: 0 101 900
Size: 101 799
ScaI AGT'ACT
Cuts at: 0 381 900
Size: 381 519
SfcI C'TryA_G
Cuts at: 0 112 313 900
Size: 112 201 587
Fragments arranged by size:
587 201 112
SgrAI Cr'CCGG_yG
Cuts at: 0 203 900
Size: 203 697
SspI AAT'ATT
Cuts at: 0 502 900
Size: 502 398
StuI AGG'CCT
Cuts at: 0 335 900
Size: 335 565
TatI w'GTAC_w
Cuts at: 0 379 900
Size: 379 521
Tth111I GACn'n_nGTC
Cuts at: 0 424 900
Size: 424 476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 900
Size: 252 648
ZraI GAC'GTC
Cuts at: 0 747 900
Size: 747 153
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI Bme1580I BmrI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrFI BstAPI BstYI EarI Eco57MI EcoO109I
FalI Hin4I MmeI NspI PciI PflMI PpuMI PspOMI
PstI SapI ScaI SfcI SgrAI SspI StuI TatI
Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI
BseYI BsiEI BsiWI BsmBI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StyI SwaI TaqII TstI XbaI XhoI XmaI
XmnI