(Linear) (Six-base) MAPSORT of: KIEE9607.seq  Check: 2677  from: 1  to: 804



KIEE9607.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:33  ..

AccI GT'mk_AC

Cuts at:      0     417     804 
   Size:        417     387
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     358     655     804 
   Size:        173     185     297     149

  Fragments arranged by size: 

                 297     185     173     149
 
AlwNI CAG_nnn'CTG

Cuts at:      0     555     804 
   Size:        555     249
 
ApoI r'AATT_y

Cuts at:      0     545     680     804 
   Size:        545     135     124

  Fragments arranged by size: 

                 545     135     124
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     392     425     804 
   Size:        392      33     379

  Fragments arranged by size: 

                 392     379      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     140     804 
   Size:        140     664
 
BfrBI ATG'CAT

Cuts at:      0     600     799     804 
   Size:        600     199       5

  Fragments arranged by size: 

                 600     199       5
 
BglII A'GATC_T

Cuts at:      0     141     804 
   Size:        141     663
 
Bme1580I G_kGCm'C

Cuts at:      0     438     804 
   Size:        438     366
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527     804 
   Size:        527     277
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     236     493     804 
   Size:        236     257     311

  Fragments arranged by size: 

                 311     257     236
 
BsaWI w'CCGG_w

Cuts at:      0     633     804 
   Size:        633     171
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     595     804 
   Size:        595     209
 
BsmI GAATG_Cn'

Cuts at:      0     711     804 
   Size:        711      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     438     804 
   Size:        438     366
 
BspEI T'CCGG_A

Cuts at:      0     633     804 
   Size:        633     171
 
BssSI C'ACGA_G

Cuts at:      0     437     804 
   Size:        437     367
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     390     804 
   Size:        390     414
 
BstYI r'GATC_y

Cuts at:      0     141     804 
   Size:        141     663
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     548     583     804 
   Size:        548      35     221

  Fragments arranged by size: 

                 548     221      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     572     635     716     748     804 
   Size:        572      63      81      32      56

  Fragments arranged by size: 

                 572      81      63      56      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     358     655     804 
   Size:        173     185     297     149

  Fragments arranged by size: 

                 297     185     173     149
 
HaeII r_GCGC'y

Cuts at:      0     691     804 
   Size:        691     113
 
HincII GTy'rAC

Cuts at:      0     764     804 
   Size:        764      40
 
NsiI A_TGCA'T

Cuts at:      0     602     801     804 
   Size:        602     199       3

  Fragments arranged by size: 

                 602     199       3
 
NspI r_CATG'y

Cuts at:      0     762     804 
   Size:        762      42
 
SapI GCTCTTCn'nnn_

Cuts at:      0     716     748     804 
   Size:        716      32      56

  Fragments arranged by size: 

                 716      56      32
 
ScaI AGT'ACT

Cuts at:      0     623     804 
   Size:        623     181
 
SfcI C'TryA_G

Cuts at:      0     472     804 
   Size:        472     332
 
SspI AAT'ATT

Cuts at:      0     525     804 
   Size:        525     279
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     678     804 
   Size:        678     126
 
TatI w'GTAC_w

Cuts at:      0     621     804 
   Size:        621     183
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     BaeI     BbsI    BfrBI    BglII
 Bme1580I     BmrI     BsaI    BsaWI    BseRI     BsmI Bsp1286I    BspEI
    BssSI    BstXI    BstYI    CspCI     EarI  Eco57MI    HaeII   HincII
     NsiI     NspI     SapI     ScaI     SfcI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI