Restriction map for
pFN21AE9606
(Linear) (Six-base) MAPSORT of: KIEE9606.seq Check: 7286 from: 1 to: 699
KIEE9606.orf
With 182 enzymes: SgfI *
March 22, 2012 18:33 ..
AflIII A'CryG_T
Cuts at: 0 173 449 699
Size: 173 276 250
Fragments arranged by size:
276 250 173
AleI CACnn'nnGTG
Cuts at: 0 21 699
Size: 21 678
AvaI C'yCGr_G
Cuts at: 0 114 699
Size: 114 585
BanII G_rGCy'C
Cuts at: 0 160 699
Size: 160 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 176 699
Size: 116 60 523
Fragments arranged by size:
523 116 60
BlpI GC'TnA_GC
Cuts at: 0 286 699
Size: 286 413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 699
Size: 226 473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 699
Size: 427 272
BsaAI yAC'GTr
Cuts at: 0 450 699
Size: 450 249
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 223 226 244 699
Size: 72 151 3 18 455
Fragments arranged by size:
455 151 72 18 3
BseYI C'CCAG_C
Cuts at: 0 273 290 699
Size: 273 17 409
Fragments arranged by size:
409 273 17
BsiEI CG_ry'CG
Cuts at: 0 271 699
Size: 271 428
BsiHKAI G_wGCw'C
Cuts at: 0 160 256 433 699
Size: 160 96 177 266
Fragments arranged by size:
266 177 160 96
Bsp1286I G_dGCh'C
Cuts at: 0 160 256 433 699
Size: 160 96 177 266
Fragments arranged by size:
266 177 160 96
BsrFI r'CCGG_y
Cuts at: 0 263 699
Size: 263 436
BsrGI T'GTAC_A
Cuts at: 0 452 699
Size: 452 247
BstAPI GCAn_nnn'nTGC
Cuts at: 0 35 699
Size: 35 664
EaeI y'GGCC_r
Cuts at: 0 182 268 296 699
Size: 182 86 28 403
Fragments arranged by size:
403 182 86 28
EagI C'GGCC_G
Cuts at: 0 268 699
Size: 268 431
EcoICRI GAG'CTC
Cuts at: 0 158 699
Size: 158 541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 699
Size: 226 473
EcoO109I rG'GnC_Cy
Cuts at: 0 536 699
Size: 536 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 699
Size: 507 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 699
Size: 652 32 15
Fragments arranged by size:
652 32 15
MluI A'CGCG_T
Cuts at: 0 173 699
Size: 173 526
MscI TGG'CCA
Cuts at: 0 298 699
Size: 298 401
MslI CAynn'nnrTG
Cuts at: 0 21 699
Size: 21 678
MspA1I CmG'CkG
Cuts at: 0 129 204 240 273 699
Size: 129 75 36 33 426
Fragments arranged by size:
426 129 75 36 33
NaeI GCC'GGC
Cuts at: 0 265 699
Size: 265 434
NgoMIV G'CCGG_C
Cuts at: 0 263 699
Size: 263 436
NotI GC'GGCC_GC
Cuts at: 0 268 699
Size: 268 431
NspI r_CATG'y
Cuts at: 0 153 699
Size: 153 546
PpuMI rG'GwC_Cy
Cuts at: 0 536 699
Size: 536 163
PspXI vC'TCGA_Gb
Cuts at: 0 114 699
Size: 114 585
PvuII CAG'CTG
Cuts at: 0 129 204 699
Size: 129 75 495
Fragments arranged by size:
495 129 75
SacI G_AGCT'C
Cuts at: 0 160 699
Size: 160 539
SmlI C'TyrA_G
Cuts at: 0 114 406 699
Size: 114 292 293
Fragments arranged by size:
293 292 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2 282 699
Size: 2 280 417
Fragments arranged by size:
417 280 2
TatI w'GTAC_w
Cuts at: 0 452 699
Size: 452 247
XhoI C'TCGA_G
Cuts at: 0 114 699
Size: 114 585
Enzymes that do cut:
AflIII AleI AvaI BanII BbsI BlpI BpmI BpuEI
BsaAI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrFI BsrGI
BstAPI EaeI EagI EcoICRI Eco57MI EcoO109I FalI Hin4I
MluI MscI MslI MspA1I NaeI NgoMIV NotI NspI
PpuMI PspXI PvuII SacI SmlI TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MmeI NarI NcoI NdeI NheI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI