Restriction map for
pFN21AE9604
(Linear) (Six-base) MAPSORT of: KIEE9604.seq Check: 9201 from: 1 to: 657
KIEE9604.orf
With 182 enzymes: SgfI *
March 22, 2012 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 73 657
Size: 73 584
AfeI AGC'GCT
Cuts at: 0 194 657
Size: 194 463
AleI CACnn'nnGTG
Cuts at: 0 277 657
Size: 277 380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 657
Size: 295 362
AlwNI CAG_nnn'CTG
Cuts at: 0 205 493 657
Size: 205 288 164
Fragments arranged by size:
288 205 164
ApaI G_GGCC'C
Cuts at: 0 113 657
Size: 113 544
BanI G'GyrC_C
Cuts at: 0 271 292 549 657
Size: 271 21 257 108
Fragments arranged by size:
271 257 108 21
BanII G_rGCy'C
Cuts at: 0 113 128 562 657
Size: 113 15 434 95
Fragments arranged by size:
434 113 95 15
BbeI G_GCGC'C
Cuts at: 0 553 657
Size: 553 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 463 657
Size: 55 408 194
Fragments arranged by size:
408 194 55
Bme1580I G_kGCm'C
Cuts at: 0 113 274 295 657
Size: 113 161 21 362
Fragments arranged by size:
362 161 113 21
BmgBI CAC'GTC
Cuts at: 0 216 657
Size: 216 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 657
Size: 515 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 235 657
Size: 169 66 422
Fragments arranged by size:
422 169 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 657
Size: 253 404
BsaHI Gr'CG_yC
Cuts at: 0 550 657
Size: 550 107
BseYI C'CCAG_C
Cuts at: 0 243 561 657
Size: 243 318 96
Fragments arranged by size:
318 243 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 657
Size: 301 356
BsiHKAI G_wGCw'C
Cuts at: 0 128 657
Size: 128 529
Bsp1286I G_dGCh'C
Cuts at: 0 113 128 274 295 562 657
Size: 113 15 146 21 267 95
Fragments arranged by size:
267 146 113 95 21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 657
Size: 73 584
BsrBI CCG'CTC
Cuts at: 0 181 400 657
Size: 181 219 257
Fragments arranged by size:
257 219 181
BsrFI r'CCGG_y
Cuts at: 0 295 480 657
Size: 295 185 177
Fragments arranged by size:
295 185 177
Bsu36I CC'TnA_GG
Cuts at: 0 353 657
Size: 353 304
EaeI y'GGCC_r
Cuts at: 0 89 176 374 431 657
Size: 89 87 198 57 226
Fragments arranged by size:
226 198 89 87 57
EarI CTCTTCn'nnn_
Cuts at: 0 439 657
Size: 439 218
EcoICRI GAG'CTC
Cuts at: 0 126 657
Size: 126 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 657
Size: 515 142
EcoO109I rG'GnC_Cy
Cuts at: 0 110 657
Size: 110 547
HaeII r_GCGC'y
Cuts at: 0 196 452 553 657
Size: 196 256 101 104
Fragments arranged by size:
256 196 104 101
KasI G'GCGC_C
Cuts at: 0 549 657
Size: 549 108
MslI CAynn'nnrTG
Cuts at: 0 277 657
Size: 277 380
MspA1I CmG'CkG
Cuts at: 0 28 164 205 490 657
Size: 28 136 41 285 167
Fragments arranged by size:
285 167 136 41 28
NaeI GCC'GGC
Cuts at: 0 482 657
Size: 482 175
NarI GG'CG_CC
Cuts at: 0 550 657
Size: 550 107
NgoMIV G'CCGG_C
Cuts at: 0 480 657
Size: 480 177
PspOMI G'GGCC_C
Cuts at: 0 109 657
Size: 109 548
PstI C_TGCA'G
Cuts at: 0 84 489 657
Size: 84 405 168
Fragments arranged by size:
405 168 84
PvuII CAG'CTG
Cuts at: 0 490 657
Size: 490 167
SacI G_AGCT'C
Cuts at: 0 128 657
Size: 128 529
SapI GCTCTTCn'nnn_
Cuts at: 0 439 657
Size: 439 218
SfcI C'TryA_G
Cuts at: 0 80 485 657
Size: 80 405 172
Fragments arranged by size:
405 172 80
SfoI GGC'GCC
Cuts at: 0 551 657
Size: 551 106
SgrAI Cr'CCGG_yG
Cuts at: 0 295 657
Size: 295 362
SmlI C'TyrA_G
Cuts at: 0 184 250 657
Size: 184 66 407
Fragments arranged by size:
407 184 66
StyI C'CwwG_G
Cuts at: 0 152 287 377 587 617 657
Size: 152 135 90 210 30 40
Fragments arranged by size:
210 152 135 90 40 30
Enzymes that do cut:
AarI AfeI AleI AlfI AlwNI ApaI BanI BanII
BbeI BbsI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI Bsu36I
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII KasI MslI
MspA1I NaeI NarI NgoMIV PspOMI PstI PvuII SacI
SapI SfcI SfoI SgrAI SmlI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI