Restriction map for
pFN21AE9602
(Linear) (Six-base) MAPSORT of: KIEE9602.seq Check: 913 from: 1 to: 591
KIEE9602.orf
With 182 enzymes: SgfI *
March 22, 2012 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 204 591
Size: 96 108 387
Fragments arranged by size:
387 108 96
AccI GT'mk_AC
Cuts at: 0 374 591
Size: 374 217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 289 310 591
Size: 244 45 21 281
Fragments arranged by size:
281 244 45 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 591
Size: 155 436
AlwNI CAG_nnn'CTG
Cuts at: 0 39 246 591
Size: 39 207 345
Fragments arranged by size:
345 207 39
ApaLI G'TGCA_C
Cuts at: 0 217 591
Size: 217 374
ApoI r'AATT_y
Cuts at: 0 547 591
Size: 547 44
BanI G'GyrC_C
Cuts at: 0 55 95 139 591
Size: 55 40 44 452
Fragments arranged by size:
452 55 44 40
BanII G_rGCy'C
Cuts at: 0 332 591
Size: 332 259
BbeI G_GCGC'C
Cuts at: 0 59 99 591
Size: 59 40 492
Fragments arranged by size:
492 59 40
BglI GCCn_nnn'nGGC
Cuts at: 0 53 591
Size: 53 538
BlpI GC'TnA_GC
Cuts at: 0 43 591
Size: 43 548
Bme1580I G_kGCm'C
Cuts at: 0 221 591
Size: 221 370
BmrI ACTGGGnnnn_n'
Cuts at: 0 494 591
Size: 494 97
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 591
Size: 181 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 525 588 591
Size: 373 152 63 3
Fragments arranged by size:
373 152 63 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 591
Size: 316 275
BsaAI yAC'GTr
Cuts at: 0 414 591
Size: 414 177
BsaHI Gr'CG_yC
Cuts at: 0 56 96 591
Size: 56 40 495
Fragments arranged by size:
495 56 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 289 591
Size: 37 252 302
Fragments arranged by size:
302 252 37
BseYI C'CCAG_C
Cuts at: 0 51 90 253 268 509 533 591
Size: 51 39 163 15 241 24 58
Fragments arranged by size:
241 163 58 51 39 24 15
BsiEI CG_ry'CG
Cuts at: 0 407 591
Size: 407 184
BsiHKAI G_wGCw'C
Cuts at: 0 221 332 591
Size: 221 111 259
Fragments arranged by size:
259 221 111
Bsp1286I G_dGCh'C
Cuts at: 0 221 332 591
Size: 221 111 259
Fragments arranged by size:
259 221 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 96 204 591
Size: 96 108 387
Fragments arranged by size:
387 108 96
BsrBI CCG'CTC
Cuts at: 0 117 591
Size: 117 474
BssSI C'ACGA_G
Cuts at: 0 100 591
Size: 100 491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 522 591
Size: 321 201 69
Fragments arranged by size:
321 201 69
BstYI r'GATC_y
Cuts at: 0 161 348 591
Size: 161 187 243
Fragments arranged by size:
243 187 161
BtgI C'CryG_G
Cuts at: 0 170 591
Size: 170 421
DraIII CAC_nnn'GTG
Cuts at: 0 587 591
Size: 587 4
DrdI GACnn_nn'nnGTC
Cuts at: 0 388 591
Size: 388 203
EaeI y'GGCC_r
Cuts at: 0 2 404 591
Size: 2 402 187
Fragments arranged by size:
402 187 2
EagI C'GGCC_G
Cuts at: 0 404 591
Size: 404 187
EarI CTCTTCn'nnn_
Cuts at: 0 320 591
Size: 320 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 361 591
Size: 361 230
EcoICRI GAG'CTC
Cuts at: 0 330 591
Size: 330 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 289 310 373 525 588 591
Size: 244 45 21 63 152 63 3
Fragments arranged by size:
244 152 63 63 45 21 3
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 274 591
Size: 274 317
HaeII r_GCGC'y
Cuts at: 0 59 99 591
Size: 59 40 492
Fragments arranged by size:
492 59 40
HincII GTy'rAC
Cuts at: 0 375 591
Size: 375 216
KasI G'GCGC_C
Cuts at: 0 55 95 591
Size: 55 40 496
Fragments arranged by size:
496 55 40
MspA1I CmG'CkG
Cuts at: 0 153 246 591
Size: 153 93 345
Fragments arranged by size:
345 153 93
NarI GG'CG_CC
Cuts at: 0 56 96 591
Size: 56 40 495
Fragments arranged by size:
495 56 40
NcoI C'CATG_G
Cuts at: 0 170 591
Size: 170 421
PfoI T'CCnGG_A
Cuts at: 0 541 591
Size: 541 50
PshAI GACnn'nnGTC
Cuts at: 0 284 591
Size: 284 307
PstI C_TGCA'G
Cuts at: 0 242 591
Size: 242 349
PvuII CAG'CTG
Cuts at: 0 153 246 591
Size: 153 93 345
Fragments arranged by size:
345 153 93
SacI G_AGCT'C
Cuts at: 0 332 591
Size: 332 259
SalI G'TCGA_C
Cuts at: 0 373 591
Size: 373 218
SapI GCTCTTCn'nnn_
Cuts at: 0 320 591
Size: 320 271
SfcI C'TryA_G
Cuts at: 0 238 591
Size: 238 353
SfoI GGC'GCC
Cuts at: 0 57 97 591
Size: 57 40 494
Fragments arranged by size:
494 57 40
SmlI C'TyrA_G
Cuts at: 0 331 591
Size: 331 260
StyI C'CwwG_G
Cuts at: 0 170 591
Size: 170 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 591
Size: 547 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 445 477 571 591
Size: 445 32 94 20
Fragments arranged by size:
445 94 32 20
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaLI ApoI BanI
BanII BbeI BglI BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BssSI BstXI BstYI BtgI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI FalI HaeII
HincII KasI MspA1I NarI NcoI PfoI PshAI PstI
PvuII SacI SalI SapI SfcI SfoI SmlI StyI
TaqII TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI Bpu10I BsaI BsaBI BsaWI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI