(Linear) (Six-base) MAPSORT of: KIEE9601.seq  Check: 1140  from: 1  to: 561



KIEE9601.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 19:02  ..

AflIII A'CryG_T

Cuts at:      0     494     561 
   Size:        494      67
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279     561 
   Size:        279     282
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     302     561 
   Size:        302     259
 
BglII A'GATC_T

Cuts at:      0     141     561 
   Size:        141     420
 
BlpI GC'TnA_GC

Cuts at:      0     280     561 
   Size:        280     281
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     245     561 
   Size:        245     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     342     561 
   Size:        342     219
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     206     561 
   Size:        206     355
 
BseYI C'CCAG_C

Cuts at:      0     481     561 
   Size:        481      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     561 
   Size:        272     289
 
BsrGI T'GTAC_A

Cuts at:      0     497     561 
   Size:        497      64
 
BstYI r'GATC_y

Cuts at:      0     141     561 
   Size:        141     420
 
BtsI GCAGTG_nn'

Cuts at:      0     260     561 
   Size:        260     301
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     439     561 
   Size:        439     122
 
HaeII r_GCGC'y

Cuts at:      0     329     561 
   Size:        329     232
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260     561 
   Size:        228      32     301

  Fragments arranged by size: 

                 301     228      32
 
HincII GTy'rAC

Cuts at:      0     236     561 
   Size:        236     325
 
MslI CAynn'nnrTG

Cuts at:      0     248     505     561 
   Size:        248     257      56

  Fragments arranged by size: 

                 257     248      56
 
MspA1I CmG'CkG

Cuts at:      0     276     561 
   Size:        276     285
 
NspI r_CATG'y

Cuts at:      0     498     561 
   Size:        498      63
 
PciI A'CATG_T

Cuts at:      0     494     561 
   Size:        494      67
 
PvuII CAG'CTG

Cuts at:      0     276     561 
   Size:        276     285
 
SfcI C'TryA_G

Cuts at:      0     155     561 
   Size:        155     406
 
SmlI C'TyrA_G

Cuts at:      0     321     561 
   Size:        321     240
 
StuI AGG'CCT

Cuts at:      0     136     561 
   Size:        136     425
 
TatI w'GTAC_w

Cuts at:      0     497     536     561 
   Size:        497      39      25

  Fragments arranged by size: 

                 497      39      25
 

 Enzymes that do cut:

   AflIII    AlwNI     BbsI    BglII     BlpI     BplI    BpuEI    BseRI
    BseYI     BsgI    BsrGI    BstYI     BtsI     FalI    HaeII    Hin4I
   HincII     MslI   MspA1I     NspI     PciI    PvuII     SfcI     SmlI
     StuI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI