Restriction map for
pFN21AE9577
(Linear) (Six-base) MAPSORT of: KIEE9577.seq Check: 3351 from: 1 to: 1629
KIEE9577.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1013 1386 1629
Size: 1013 373 243
Fragments arranged by size:
1013 373 243
AccI GT'mk_AC
Cuts at: 0 1364 1629
Size: 1364 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 1030 1342 1629
Size: 597 433 312 287
Fragments arranged by size:
597 433 312 287
AflIII A'CryG_T
Cuts at: 0 854 1629
Size: 854 775
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1558 1590 1629
Size: 1189 32 337 32 39
Fragments arranged by size:
1189 337 39 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1128 1425 1629
Size: 1128 297 204
Fragments arranged by size:
1128 297 204
AvaI C'yCGr_G
Cuts at: 0 293 421 1629
Size: 293 128 1208
Fragments arranged by size:
1208 293 128
BanI G'GyrC_C
Cuts at: 0 1015 1171 1231 1629
Size: 1015 156 60 398
Fragments arranged by size:
1015 398 156 60
BanII G_rGCy'C
Cuts at: 0 26 40 428 458 1450 1629
Size: 26 14 388 30 992 179
Fragments arranged by size:
992 388 179 30 26 14
BbeI G_GCGC'C
Cuts at: 0 1019 1175 1629
Size: 1019 156 454
Fragments arranged by size:
1019 454 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 409 858 1629
Size: 409 449 771
Fragments arranged by size:
771 449 409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 995 1629
Size: 961 34 634
Fragments arranged by size:
961 634 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1138 1629
Size: 1138 491
Bme1580I G_kGCm'C
Cuts at: 0 1409 1629
Size: 1409 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 932 1629
Size: 932 697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 301 1255 1629
Size: 199 102 954 374
Fragments arranged by size:
954 374 199 102
Bpu10I CC'TnA_GC
Cuts at: 0 586 1050 1629
Size: 586 464 579
Fragments arranged by size:
586 579 464
BsaAI yAC'GTr
Cuts at: 0 624 857 1185 1629
Size: 624 233 328 444
Fragments arranged by size:
624 444 328 233
BsaBI GATnn'nnATC
Cuts at: 0 1111 1629
Size: 1111 518
BsaHI Gr'CG_yC
Cuts at: 0 1016 1172 1629
Size: 1016 156 457
Fragments arranged by size:
1016 457 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 440 470 1189 1219 1629
Size: 172 30 238 30 719 30 410
Fragments arranged by size:
719 410 238 172 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 418 910 1012 1549 1629
Size: 30 388 492 102 537 80
Fragments arranged by size:
537 492 388 102 80 30
BseYI C'CCAG_C
Cuts at: 0 204 1055 1629
Size: 204 851 574
Fragments arranged by size:
851 574 204
BsiEI CG_ry'CG
Cuts at: 0 30 1629
Size: 30 1599
BsiHKAI G_wGCw'C
Cuts at: 0 1472 1629
Size: 1472 157
Bsp1286I G_dGCh'C
Cuts at: 0 26 40 428 458 1409 1450 1472 1629
Size: 26 14 388 30 951 41 22 157
Fragments arranged by size:
951 388 157 41 30 26 22 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 1013 1113 1386 1520 1629
Size: 363 650 100 273 134 109
Fragments arranged by size:
650 363 273 134 109 100
BsrBI CCG'CTC
Cuts at: 0 133 1629
Size: 133 1496
BsrFI r'CCGG_y
Cuts at: 0 104 1629
Size: 104 1525
BstYI r'GATC_y
Cuts at: 0 366 872 1629
Size: 366 506 757
Fragments arranged by size:
757 506 366
BtgI C'CryG_G
Cuts at: 0 30 41 1629
Size: 30 11 1588
Fragments arranged by size:
1588 30 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 981 1020 1365 1629
Size: 981 39 345 264
Fragments arranged by size:
981 345 264 39
BtsI GCAGTG_nn'
Cuts at: 0 11 545 1310 1426 1629
Size: 11 534 765 116 203
Fragments arranged by size:
765 534 203 116 11
DraIII CAC_nnn'GTG
Cuts at: 0 627 1629
Size: 627 1002
EaeI y'GGCC_r
Cuts at: 0 27 815 1130 1313 1629
Size: 27 788 315 183 316
Fragments arranged by size:
788 316 315 183 27
EagI C'GGCC_G
Cuts at: 0 27 1629
Size: 27 1602
EarI CTCTTCn'nnn_
Cuts at: 0 218 928 1030 1629
Size: 218 710 102 599
Fragments arranged by size:
710 599 218 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 512 658 792 1629
Size: 512 146 134 837
Fragments arranged by size:
837 512 146 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 301 597 1030 1255 1342 1629
Size: 199 102 296 433 225 87 287
Fragments arranged by size:
433 296 287 225 199 102 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 1009 1393 1629
Size: 214 795 384 236
Fragments arranged by size:
795 384 236 214
EcoO109I rG'GnC_Cy
Cuts at: 0 503 550 570 1125 1629
Size: 503 47 20 555 504
Fragments arranged by size:
555 504 503 47 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1116 1629
Size: 1116 513
HaeII r_GCGC'y
Cuts at: 0 115 1019 1175 1629
Size: 115 904 156 454
Fragments arranged by size:
904 454 156 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 534 566 621 653 1629
Size: 122 32 380 32 55 32 976
Fragments arranged by size:
976 380 122 55 32 32 32
HincII GTy'rAC
Cuts at: 0 530 1599 1629
Size: 530 1069 30
Fragments arranged by size:
1069 530 30
KasI G'GCGC_C
Cuts at: 0 1015 1171 1629
Size: 1015 156 458
Fragments arranged by size:
1015 458 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1629
Size: 1061 568
MscI TGG'CCA
Cuts at: 0 1132 1315 1629
Size: 1132 183 314
Fragments arranged by size:
1132 314 183
MslI CAynn'nnrTG
Cuts at: 0 721 724 796 1340 1629
Size: 721 3 72 544 289
Fragments arranged by size:
721 544 289 72 3
MspA1I CmG'CkG
Cuts at: 0 32 43 57 144 766 820 1518 1613
Size: 32 11 14 87 622 54 698 95
Cuts at: 1613 1629
Size: 16
Fragments arranged by size:
698 622 95 87 54 32 16 14
11
NarI GG'CG_CC
Cuts at: 0 1016 1172 1629
Size: 1016 156 457
Fragments arranged by size:
1016 457 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1437 1629
Size: 108 1329 192
Fragments arranged by size:
1329 192 108
PfoI T'CCnGG_A
Cuts at: 0 212 302 431 731 1271 1577 1629
Size: 212 90 129 300 540 306 52
Fragments arranged by size:
540 306 300 212 129 90 52
PmlI CAC'GTG
Cuts at: 0 624 1629
Size: 624 1005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1629
Size: 1189 32 408
Fragments arranged by size:
1189 408 32
PpuMI rG'GwC_Cy
Cuts at: 0 503 550 570 1125 1629
Size: 503 47 20 555 504
Fragments arranged by size:
555 504 503 47 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 1629
Size: 1492 32 105
Fragments arranged by size:
1492 105 32
PstI C_TGCA'G
Cuts at: 0 374 513 850 1397 1517 1629
Size: 374 139 337 547 120 112
Fragments arranged by size:
547 374 337 139 120 112
PvuII CAG'CTG
Cuts at: 0 144 1518 1629
Size: 144 1374 111
Fragments arranged by size:
1374 144 111
SacII CC_GC'GG
Cuts at: 0 33 44 1629
Size: 33 11 1585
Fragments arranged by size:
1585 33 11
SexAI A'CCwGG_T
Cuts at: 0 572 1629
Size: 572 1057
SfcI C'TryA_G
Cuts at: 0 370 509 846 1393 1513 1629
Size: 370 139 337 547 120 116
Fragments arranged by size:
547 370 337 139 120 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1138 1629
Size: 1138 491
SfoI GGC'GCC
Cuts at: 0 1017 1173 1629
Size: 1017 156 456
Fragments arranged by size:
1017 456 156
SmaI CCC'GGG
Cuts at: 0 295 423 1629
Size: 295 128 1206
Fragments arranged by size:
1206 295 128
StuI AGG'CCT
Cuts at: 0 977 1586 1629
Size: 977 609 43
Fragments arranged by size:
977 609 43
StyI C'CwwG_G
Cuts at: 0 362 893 1629
Size: 362 531 736
Fragments arranged by size:
736 531 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 254 822 1629
Size: 70 184 568 807
Fragments arranged by size:
807 568 184 70
TatI w'GTAC_w
Cuts at: 0 656 989 1629
Size: 656 333 640
Fragments arranged by size:
656 640 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1125 1279 1629
Size: 174 951 154 350
Fragments arranged by size:
951 350 174 154
XmaI C'CCGG_G
Cuts at: 0 293 421 1629
Size: 293 128 1208
Fragments arranged by size:
1208 293 128
XmnI GAAnn'nnTTC
Cuts at: 0 151 1327 1629
Size: 151 1176 302
Fragments arranged by size:
1176 302 151
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI AvaI BanI
BanII BbeI BbsI BcgI BglI Bme1580I BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI PflMI PfoI PmlI PpiI PpuMI
PsrI PstI PvuII SacII SexAI SfcI SfiI SfoI
SmaI StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI ZraI