Restriction map for
pFN21AE9571
(Linear) (Six-base) MAPSORT of: KIEE9571.seq Check: 9222 from: 1 to: 1224
KIEE9571.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AccI GT'mk_AC
Cuts at: 0 668 1224
Size: 668 556
AfeI AGC'GCT
Cuts at: 0 454 562 1224
Size: 454 108 662
Fragments arranged by size:
662 454 108
AflIII A'CryG_T
Cuts at: 0 218 1224
Size: 218 1006
ApaI G_GGCC'C
Cuts at: 0 126 870 958 976 1224
Size: 126 744 88 18 248
Fragments arranged by size:
744 248 126 88 18
ApaLI G'TGCA_C
Cuts at: 0 873 1224
Size: 873 351
AvaI C'yCGr_G
Cuts at: 0 739 969 1143 1224
Size: 739 230 174 81
Fragments arranged by size:
739 230 174 81
BanI G'GyrC_C
Cuts at: 0 60 270 316 756 813 895 1224
Size: 60 210 46 440 57 82 329
Fragments arranged by size:
440 329 210 82 60 57 46
BanII G_rGCy'C
Cuts at: 0 126 870 958 976 1112 1224
Size: 126 744 88 18 136 112
Fragments arranged by size:
744 136 126 112 88 18
BbeI G_GCGC'C
Cuts at: 0 64 320 760 1224
Size: 64 256 440 464
Fragments arranged by size:
464 440 256 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 1066 1224
Size: 1066 158
BglI GCCn_nnn'nGGC
Cuts at: 0 130 289 1088 1129 1224
Size: 130 159 799 41 95
Fragments arranged by size:
799 159 130 95 41
Bme1580I G_kGCm'C
Cuts at: 0 126 818 870 877 898 958 976 1224
Size: 126 692 52 7 21 60 18 248
Fragments arranged by size:
692 248 126 60 52 21 18 7
BmgBI CAC'GTC
Cuts at: 0 351 794 1224
Size: 351 443 430
Fragments arranged by size:
443 430 351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1224
Size: 42 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1224
Size: 187 1037
BsaI GGTCTCn'nnnn_
Cuts at: 0 889 1224
Size: 889 335
BsaAI yAC'GTr
Cuts at: 0 612 1224
Size: 612 612
BsaHI Gr'CG_yC
Cuts at: 0 61 317 548 757 1224
Size: 61 256 231 209 467
Fragments arranged by size:
467 256 231 209 61
BsaWI w'CCGG_w
Cuts at: 0 1104 1224
Size: 1104 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 783 813 1224
Size: 783 30 411
Fragments arranged by size:
783 411 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 757 1224
Size: 615 142 467
Fragments arranged by size:
615 467 142
BseYI C'CCAG_C
Cuts at: 0 723 1170 1224
Size: 723 447 54
Fragments arranged by size:
723 447 54
BsiEI CG_ry'CG
Cuts at: 0 497 828 1092 1224
Size: 497 331 264 132
Fragments arranged by size:
497 331 264 132
BsiHKAI G_wGCw'C
Cuts at: 0 877 1224
Size: 877 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 636 1224
Size: 636 588
Bsp1286I G_dGCh'C
Cuts at: 0 126 818 870 877 898 958 976 1112
Size: 126 692 52 7 21 60 18 136
Cuts at: 1112 1224
Size: 112
Fragments arranged by size:
692 136 126 112 60 52 21 18
7
BspEI T'CCGG_A
Cuts at: 0 1104 1224
Size: 1104 120
BspHI T'CATG_A
Cuts at: 0 518 1224
Size: 518 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 1224
Size: 173 1051
BsrBI CCG'CTC
Cuts at: 0 1029 1224
Size: 1029 195
BsrFI r'CCGG_y
Cuts at: 0 276 753 827 1224
Size: 276 477 74 397
Fragments arranged by size:
477 397 276 74
BssHII G'CGCG_C
Cuts at: 0 838 942 1224
Size: 838 104 282
Fragments arranged by size:
838 282 104
BssSI C'ACGA_G
Cuts at: 0 16 652 1224
Size: 16 636 572
Fragments arranged by size:
636 572 16
BstEII G'GTnAC_C
Cuts at: 0 444 1224
Size: 444 780
BtgI C'CryG_G
Cuts at: 0 7 37 100 152 395 497 1224
Size: 7 30 63 52 243 102 727
Fragments arranged by size:
727 243 102 63 52 30 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 168 1224
Size: 168 1056
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 1224
Size: 63 35 1126
Fragments arranged by size:
1126 63 35
EaeI y'GGCC_r
Cuts at: 0 40 146 179 236 281 300 307 392
Size: 40 106 33 57 45 19 7 85
Cuts at: 392 494 825 929 1089 1224
Size: 102 331 104 160 135
Fragments arranged by size:
331 160 135 106 104 102 85 57
45 40 33 19 7
EagI C'GGCC_G
Cuts at: 0 494 825 1089 1224
Size: 494 331 264 135
Fragments arranged by size:
494 331 264 135
EarI CTCTTCn'nnn_
Cuts at: 0 653 667 1141 1224
Size: 653 14 474 83
Fragments arranged by size:
653 474 83 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 725 740 791 1057 1224
Size: 725 15 51 266 167
Fragments arranged by size:
725 266 167 51 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1224
Size: 187 1037
EcoO109I rG'GnC_Cy
Cuts at: 0 122 123 867 954 955 1224
Size: 122 1 744 87 1 269
Fragments arranged by size:
744 269 122 87 1 1
HaeII r_GCGC'y
Cuts at: 0 64 136 295 320 456 564 760 1064
Size: 64 72 159 25 136 108 196 304
Cuts at: 1064 1224
Size: 160
Fragments arranged by size:
304 196 160 159 136 108 72 64
25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 783 815 1224
Size: 783 32 409
Fragments arranged by size:
783 409 32
KasI G'GCGC_C
Cuts at: 0 60 316 756 1224
Size: 60 256 440 468
Fragments arranged by size:
468 440 256 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 623 1224
Size: 596 27 601
Fragments arranged by size:
601 596 27
MscI TGG'CCA
Cuts at: 0 148 181 302 931 1224
Size: 148 33 121 629 293
Fragments arranged by size:
629 293 148 121 33
MspA1I CmG'CkG
Cuts at: 0 426 469 499 702 1085 1224
Size: 426 43 30 203 383 139
Fragments arranged by size:
426 383 203 139 43 30
NaeI GCC'GGC
Cuts at: 0 278 755 829 1224
Size: 278 477 74 395
Fragments arranged by size:
477 395 278 74
NarI GG'CG_CC
Cuts at: 0 61 317 757 1224
Size: 61 256 440 467
Fragments arranged by size:
467 440 256 61
NcoI C'CATG_G
Cuts at: 0 37 1224
Size: 37 1187
NgoMIV G'CCGG_C
Cuts at: 0 276 753 827 1224
Size: 276 477 74 397
Fragments arranged by size:
477 397 276 74
NspI r_CATG'y
Cuts at: 0 606 1224
Size: 606 618
PasI CC'CwG_GG
Cuts at: 0 948 1224
Size: 948 276
PflMI CCAn_nnn'nTGG
Cuts at: 0 188 1224
Size: 188 1036
PfoI T'CCnGG_A
Cuts at: 0 854 1224
Size: 854 370
PspOMI G'GGCC_C
Cuts at: 0 122 866 954 972 1224
Size: 122 744 88 18 252
Fragments arranged by size:
744 252 122 88 18
PvuII CAG'CTG
Cuts at: 0 469 702 1224
Size: 469 233 522
Fragments arranged by size:
522 469 233
SacII CC_GC'GG
Cuts at: 0 500 1224
Size: 500 724
SapI GCTCTTCn'nnn_
Cuts at: 0 667 1224
Size: 667 557
SexAI A'CCwGG_T
Cuts at: 0 248 1224
Size: 248 976
SfoI GGC'GCC
Cuts at: 0 62 318 758 1224
Size: 62 256 440 466
Fragments arranged by size:
466 440 256 62
SgrAI Cr'CCGG_yG
Cuts at: 0 276 753 1224
Size: 276 477 471
Fragments arranged by size:
477 471 276
SmaI CCC'GGG
Cuts at: 0 971 1224
Size: 971 253
StyI C'CwwG_G
Cuts at: 0 37 287 878 1224
Size: 37 250 591 346
Fragments arranged by size:
591 346 250 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 251 283 340 372 1224
Size: 251 32 57 32 852
Fragments arranged by size:
852 251 57 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 98 1224
Size: 98 1126
XmaI C'CCGG_G
Cuts at: 0 969 1224
Size: 969 255
Enzymes that do cut:
AccI AfeI AflIII ApaI ApaLI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BmgBI BplI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstEII BtgI BtgZI CspCI EaeI EagI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PspOMI PvuII SacII SapI SexAI SfoI SgrAI
SmaI StyI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI Bpu10I BpuEI BsaBI BsgI
BsiWI BsmI BsrDI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI ZraI